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Search Results: 1 - 10 of 461645 matches for " A;Irian "
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Bee venom treatment reduced C-reactive protein and improved follicle quality in a rat model of estradiol valerate-induced polycystic ovarian syndrome
Karimzadeh, L;Nabiuni, M;Sheikholeslami, A;Irian, S;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2012, DOI: 10.1590/S1678-91992012000400006
Abstract: polycystic ovarian syndrome (pcos) is a low grade inflammatory disease characterized by hyperandrogenemia and chronic anovulation. c-reactive protein (crp), released by adipocytes, plays a key role in pcos. apis mellifera honeybee venom (hbv) contains a variety of biologically active components with various pharmaceutical properties. this study was designed to assess the possibility of hbv application as an anti-inflammatory therapeutic agent. to induce pcos, 1 mg/100 g body weight estradiol valerate (ev) was subcutaneously (sc) injected into eight-week-old rats. after 60 days, 0.5 mg/kg hbv was administered sc for 14 consecutive days, and the results of pcos treatment were investigated. rats were then anesthetized with chloroform, and their ovaries and livers were surgically removed to determine histomorphometrical changes. testosterone and 17-β-estradiol were detected by chemiluminescence immunoassay. in order to detect serum crp, elisa kit was used in three groups of ev-induced pcos, hbv-treated pcos and control animals. thickness of the theca layer, number of cysts and the level of serum crp significantly decreased in hbv group in comparison with pcos group. moreover, corpus luteum, as a sign of ovulation, was observed in hbv-treated ovaries which were absent in pcos group. our results suggest that the beneficial effect of hbv may be mediated through its inhibitory effect on serum crp levels.
ALGUNAS CONSIDERACIONES PARA EL SACRIFICIO SANITARIO MASIVO DE AVES DE CORRAL EN BROTES DE INFLUENZA AVIAR
Alfonso,P; Percedo,María Irian; Abeledo,María Antonia; Fernández,A;
Revista de Salud Animal , 2007,
Abstract: in severe disease outbreaks, large numbers of animals may need to be killed for control purposes, which is complex. this task results in sanitary, environmental and ethical problems no completely solved worldwide. in the case of poultry industry, it is very difficult because individuals are raised at high stocking densities. in addition, the zoonotic potential of avian influenza is increased, so their slaughter on premises suspected to be ai infected, must be completed with special concern. this paper summarizes the main considerations for taking decisions regarding slaughter of poultry infected by ai virus, the methods for killing them in such cases according to the safeguarding personnel involved, the impact on the environment, animal welfare aspects as well as the proper disposal of dead birds and contaminated waste.
LA PESTE PORCINA CLáSICA EN LAS AMéRICAS Y EL CARIBE: ACTUALIDAD Y PERSPECTIVAS DE CONTROL Y ERRADICACIóN
Ferrer,E; Fonseca,O; Percedo,María Irian; Abeledo,María Antonia;
Revista de Salud Animal , 2010,
Abstract: this paper aims to analyze the current situation and plans of control and eradication of classical swine fever in the americas and the caribbean as well as the main causes that lead to disease transmission. at present the classical swine fever (csf) is recognized as a transboundary disease, remains endemic in many countries of latin america and the caribbean. in the americas region remain as affected countries: nicaragua, honduras, brazil, colombia, ecuador, bolivia, peru and venezuela. in the caribbean countries the affected ones are: cuba, haiti, and dominican republic. there are several causes which hinder the control of csf, playing a key role the political will of each country and its veterinary service. the control and eradication plans are hampered by the low availability of vaccines, inadequate control of the animal movement and the illegal trade of pigs and their products. these elements contribute to the maintenance of infection and spread of the disease from affected into free areas.
In vitro effects of fetal rat cerebrospinal fluid on viability and neuronal differentiation of PC12 cells
Mohammad Nabiuni, Javad Rasouli, Kazem Parivar, Homa M Kochesfehani, Saeid Irian, Jaleel A Miyan
Fluids and Barriers of the CNS , 2012, DOI: 10.1186/2045-8118-9-8
Abstract: In vitro assays of viability, proliferation and differentiation were carried out after incubation of PC12 cells in media with and without addition of fetal rat CSF. An MTT tetrazolium assay was used to assess cell viability and/or cell proliferation. Expression of neural differentiation markers (MAP-2 and β-III tubulin) was determined by immunocytochemistry. Formation and growth of neurites was measured by image analysis.PC12 cells differentiate into neuronal cell types when exposed to bFGF. Viability and cell proliferation of PC12 cells cultured in CSF-supplemented medium from E18 rat fetuses were significantly elevated relative to the control group. Neuronal-like outgrowths from cells appeared following the application of bFGF or CSF from E17 and E19 fetuses but not E18 or E20 CSF. Beta-III tubulin was expressed in PC12 cells cultured in any media except that supplemented with E18 CSF. MAP-2 expression was found in control cultures and in those with E17 and E19 CSF. MAP2 was located in neurites except in E17 CSF when the whole cell was positive.Fetal rat CSF supports viability and stimulates proliferation and neurogenic differentiation of PC12 cells in an age-dependent way, suggesting that CSF composition changes with age. This feature may be important in vivo for the promotion of normal brain development. There were significant differences in the effects on PC12 cells compared to primary cortical cells. This suggests there is an interaction in vivo between developmental stage of cells and the composition of CSF. The data presented here support an important, perhaps driving role for CSF composition, specifically neurotrophic factors, in neuronal survival, proliferation and differentiation. The effects of CSF on PC12 cells can thus be used to further investigate the role of CSF in driving development without the confounding issues of using primary cells.The central nervous system develops around a fluid filled tube, the neural tube. Initially the tube forms around
Laparoscopia y exploración del lado opuesto de la hernia inguinal en el ni?o
Prada-Silvy,Carlos E; Brice?o Iragorry,Leopoldo; Ordaz-Humbría,Irian;
Gaceta Médica de Caracas , 2006,
Abstract: la hernia inguinal es la patología quirúrgica más frecuente de la infancia. la incidencia de conducto peritoneo vaginal permeable del lado opuesto puede ser tan alta como el 25 % de los casos. se realizó un estudio en 75 pacientes con hernia inguinal unilateral utilizando la laparoscopia a través del saco herniario para la exploración del lado opuesto, en edades comprendidas entre 1 mes y 10 a?os. el 26 % de los casos fueron positivos para la exploración, independientemente de la edad. la exploración laparoscópica de la hernia inguinal opuesta es un método confiable, seguro, económico y de fácil realización, justificado para realizarse en los pacientes pediátricos con clínica de hernia inguinal unilateral independientemente de la edad.
SISTEMA INFORMáTICO PARA EL CONTROL DE UN BANCO DE SUEROS DE ANIMALES EN áREAS DE RIESGO DE ENTRADA DE ENFERMEDADES EXóTICAS
Sánchez,O.E; Pérez Ruano,M; Percedo,María Irian;
Revista de Salud Animal , 2007,
Abstract: a software that supports a historical database for retrospective epidemiological research was designed and implemented for managing a sera bank, given the necessity to rapidly elucidate any suspicion of introduction or dissemination of an exotic disease. for the software design, the standard procedures of operation for taking, documenting and transporting serological sera of animals were reviewed. the system was developed in microsoft access xp and structured in three main parts: a) regional structure and catalogues, to obtain the primary data; b) forms, to update the official register; c) reports, to consolidate data for information analysis and decision making.
ALGUNAS PAUTAS PARA ESTABLECER UNA ESTRATEGIA DE VIGILANCIA EPIDEMIOLóGICA DE LA INFLUENZA AVIAR
Alfonso Zamora,P; Percedo,María Irian; Abeledo,Ma. Antonia; Noda,Julia;
Revista de Salud Animal , 2008,
Abstract: the explosive and almost simultaneous appearance of avian influenza (ai) in multiple countries worldwide has been interpreted as evidence of weakness of the surveillance and early warning systems for noticing ai introduction and spread hazards. countries or regions can adopt more or less intensive surveillance systems depending on the surveillance systems pre-established, poultry industry characteristics, risk factors assessed and resources available. however, it is unquestionable the need of enhancing either passive or active surveillance. the new ai profile has increased the availability of techniques and means for surveillance whose selection has certain complexities for establishing an effective diagnostic algorithm. the prevention management and control of eventual sanitary disasters demand from the veterinary services to have an epidemiological surveillance strategy in order to increase the capacities of alert and early responses at local level. in this paper, some guidelines for organizing an avian influenza epidemiological surveillance system are pointed out.
Laparoscopia y exploración del lado opuesto de la hernia inguinal en el ni o
Carlos E Prada-Silvy,Leopoldo Brice?o Iragorry,Irian Ordaz-Humbría
Gaceta Médica de Caracas , 2006,
Abstract: La hernia inguinal es la patología quirúrgica más frecuente de la infancia. La incidencia de conducto peritoneo vaginal permeable del lado opuesto puede ser tan alta como el 25 % de los casos. Se realizó un estudio en 75 pacientes con hernia inguinal unilateral utilizando la laparoscopia a través del saco herniario para la exploración del lado opuesto, en edades comprendidas entre 1 mes y 10 a os. El 26 % de los casos fueron positivos para la exploración, independientemente de la edad. La exploración laparoscópica de la hernia inguinal opuesta es un método confiable, seguro, económico y de fácil realización, justificado para realizarse en los pacientes pediátricos con clínica de hernia inguinal unilateral independientemente de la edad.
HUMORAL RESPONSE TO DIFFERENT VACCINATION SCHEMES AGAINST CLASSICAL SWINE FEVER (CSF) SUCCESSIVELY APPLIED DURING AN OUTBREAK OF THE DISEASE
Percedo,María Irian; Alfonso,P; Frías,María Teresa; Díaz de Arce,Heydi; Barrera,Maritza; Fonseca,O; Castell,Sara;
Revista de Salud Animal , 2009,
Abstract: vaccination against classical swine fever (csf) is an essential measure for its control in endemic areas, and the chinese strain has been used with success in already disease free countries. nevertheless the complexity of the factors interacting on the epidemiology and control of csf, when the expected success is not reached with the vaccination of premises, doubts always fall on the vaccine used and the vaccination scheme applied in consecuences, many changes in vaccination scheme by the breeders are frecuently observed whitout positive influence in csf control. to clarify the csf recurrence in a herd vaccinated by a different scheme as the one recommended in the national control program, serological samples were taken to different animal groups in three moments. sample i, to evaluate the response to the vaccination scheme decided by producers (litters on 5 and 40 days); and samples ii and iii, to know its behaviour after the implementation of the scheme recommended by official veterinary service (litters at weaning, at 33 days). the presence of titres =1:50 in all litters at 3 days of age (sample i), indicated that primo-vaccination at 5 days is not correct. the absence of protective titres on 22% of the animals (9/41), belonging to the groups with clinical cases of the disease, at only 23-30 days from the second doses, confirmed that the double vaccination scheme at 5 and 40 days was not correct. although at 33 days of age a risk of vaccinating in the presence of high titres of passive antibodies (=1:50) in 65% of the pigs was present (sampling ii), it was verified that at 37 post-vaccination days all pigs showed high neutralizing antibodies titres (sampling iii). this result, together with the clinical behaviour and humoral response of pigs on pre- fattening demonstrated that primo-vaccination at 33 days was adequate and contributed to the control of clinical manifestation of the diseases in the herd.
ASSESSING THE ANTIOXIDANT AND ANTICARCINOGENIC ACTIVITIES OF VIRGIN OLIVE OIL AND PURIFIED OLIVE OIL SAMPLES TREATED WITH LIGHT AND HEAT USING THE AMES TEST
Arash Shams, Sedigheh Mehrabian and Saeed Irian
International Journal of Microbiology Research , 2012,
Abstract: Chemical compounds present in fruits and vegetables are involved in combating a number of life-threatening diseases such as cancer, cataract, and cardiovascular and cerebral disorders. Identifying the antimutagenic compounds from plant sources, and evaluating their beneficial properties is an effective step in exalting the human health. Olive oil as a main source of dietary lipids, despite having high levels of unsaturated fatty acids, contains biological compounds such as the phenolic antioxidants with preventative effects against the destructive properties of free radicals and their mutagenic effects on the cellular structures. This study shows the effects of light and temperature on the antioxidant and anticancer properties of purified virgin olive oil using the Ames test. A total of 16 Iranian and Spanish oil samples were used. The antimutagenic activity assay was based on the Ames test and applied the Salmonella typhimurium TA100 mutant line along with the chemical carcinogen sodium azide, while mouse hepatic microsomes were used for the anticarcinogenic assessments. Each assay was performed in triplicates simultaneously, and the percentage of inhibition was determined using the formula (1-T/M) x 100. The highest inhibition percentages with respect to the olive variety were recorded as 63.64%, 60.70% and 46.36% for oils treated with dark, light, and light + temperature conditions, respectively. Our results indicate that both light and temperature decrease the antioxidant and anticarcinogenic activities of olive oil.
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