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Search Results: 1 - 10 of 714402 matches for " A. M.;Albas "
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Evaluación de la calidad de los reactivos que se utilizan en la tecnica de contrainmunoelectroforesis para la determinación de anticuerpos antirrábicos
Díaz, A. M.;Albas, A.;Valentini, E. J. G.;Perdomo, G.;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1991, DOI: 10.1590/S0036-46651991000100009
Abstract: this study demonstrated that the antigens and indicator sera produced by the butantan institute may be employed with success in the counterimmunoelectrophoresis technique for the titration of rabies antibodies in sera from immunized individuals. no statistically significant differences were demonstrated between the results obtained in the standardization tests carried out at the butantan institute and the reference control tests performed at the pan american zoonoses center. it is proposed that the butantan institute be in charge of the production and distribution of these reagents at the national level.
Interval between first dose and booster affected antibody production in cattle vaccinated against rabies
Albas, A.;Fontolan, O. L.;Pardo, P. E.;Bremer Neto, H.;Sartori, A.;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2006, DOI: 10.1590/S1678-91992006000300010
Abstract: in this study, we compared the levels of neutralizing antibodies induced by inactivated rabies vaccine in cattle by using three alternative immunization procedures. forty-five bovines (breed nelore) were then organized in three groups (a, b and c, with 15 animals/group). group a received only one vaccine dose at day zero and group b received the first dose at day zero and then another dose at day 30 (early booster). group c was also immunized with two doses; however, the booster was postponed until day 180 after the first dose (delayed booster). blood samples were withdrawn at days zero (before the first dose) and 30, 210, 390, and 540 after the beginning of immunization and the antibody titers were evaluated by mouse neutralization test. the protocol used to immunize group c (booster at day 180) was clearly more efficient. in this group, antibody levels were higher and also remained higher for longer periods in comparison with the other two groups. these results show that booster timing significantly affected antibody levels. therefore, programs addressed to control this disease in cattle should consider not only the use of a booster but also its administration time.
Rabies virus in a pregnant naturally infected southern yellow bat (Lasiurus ega)
Allendorf, SD;Albas, A;Cipriano, JRB;Antunes, JMAP;Appolinário, CM;Peres, MG;da Rosa, AR;Sodré, MM;Megid, J;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2011, DOI: 10.1590/S1678-91992011000200014
Abstract: current knowledge on bat lyssavirus infections in their native hosts is limited and little is known about the virulence, virus dissemination and transmission among free-living insectivorous bats. the present study is a brief description of rabies virus (rabv) dissemination in tissues of a naturally infected pregnant southern yellow bat (lasiurus ega) and its fetuses, obtained by reverse-transcriptase polymerase chain reaction (rt-pcr). the rt-pcr was positive in samples from the brain, salivary gland, tongue, lungs, heart, kidneys and liver. on the other hand, the placenta, three fetuses, spleen, intestine and brown fat tissue tested negative. this research demonstrated the absence of rabies virus in the fetuses, thus, in this specific case, the transplacentary transmission was not observed.
Vírus da raiva em quirópteros naturalmente infectados no Estado de S?o Paulo, Brasil
Scheffer,Karin Corrêa; Carrieri,Maria Luiza; Albas,Avelino; Santos,Helaine Cristina Pires dos; Kotait,Ivanete; Ito,Fumio Honma;
Revista de Saúde Pública , 2007, DOI: 10.1590/S0034-89102007000300010
Abstract: objective: to identify the species of bats involved in maintaining the rabies cycle; to investigate the distribution of the rabies virus in the tissues and organs of bats and the time taken for mortality among inoculated mice. methods: from april 2002 to november 2003, bats from municipalities in the state of s?o paulo were screened for the presence of the rabies virus, by means of direct immunofluorescence. the virus distribution in the bats was evaluated by inoculating mice and n2a cells with 20% suspensions prepared from fragments of different organs and tissues, plus the brain and salivary glands. the time taken for mortality among the mice was monitored daily, following intracerebral inoculation. results: out of the 4,395 bats received, 1.9% were found positive for the rabies virus. they belonged to ten genera, with predominance of insectivores. the maximum mean times taken for mortality among the mice following inoculation with brain and salivary gland material were 15.33±2.08 days and 11.33±2.30 days for vampire bats, 16.45±4.48 days and 18.91±6.12 days for insectivorous bats, and 12.60±2.13 days and 15.67±4.82 days for frugivorous bats, respectively. conclusions: the species infected with the rabies virus were: artibeus lituratus, artibeus sp., myotis nigricans, myotis sp., eptesicus sp., lasiurus ega, lasiurus cinereus, nyctinomops laticaudatus, tadarida brasiliensis, histiotus velatus, molossus rufus, eumops sp. and desmodus rotundus. virus investigation in the different tissues and organs showed that the brain and salivary glands were the most suitable sites for virus isolation.
Suplementa??o com levedura de cr?mio eleva a concentra??o sérica de cr?mio em bovinos
Giometti,J.; Chiacchio,S.B.; Albas,A.; Pardo,P.E.; Bremer Neto,H.; Giometti,A.I.; Reis,L.S.L.S.;
Archivos de Zootecnia , 2011, DOI: 10.4321/S0004-05922011000300071
Abstract: this study evaluated the effect of daily chromium supplementation at growing doses on serum cr levels in cattle. sixty nellore heifers 2 to 5 years old were randomly assigned to one of four groups (15 heifers/group) fed non-fertilized brachiaria decumbens pasture. in the control group (gc), the heifers received a mineral mixture without cr. in the other groups, heifers received 0.59, 1.19 or 2.38 mg cr+3/animal/day, groups g8,5, g17 and g34, respectively. blood samples were collected on days 0, 30 and 60. serum concentration of chromium was determined by graphite furnace atomic absorption spectrophotometer. the chromium source used was the chromium yeast. the results do not support any significant difference (p>0.05) among serum chromium levels of heifers in groups gc, g8,5, g17 and g34 at a same observation day. only effect of supplementation time was detected in group g34. in this group there was significant increase (p<0,05) in the concentration serum chromium 60 days after the beginning of the experiment. concluded cr yeast can be used as the source of this mineral element for animals and supplementation with 2.38 mg/ cattle/day was the dose that most increased the serum concentration of cr in nellore cattle during the experimental period.
Termoestabilidade da vacina contra a raiva, tipo Fuenzalida & Palacios, uso humano
Albas, Avelino;Mour?o Fuches, Regina Maria;Frazatti Gallina, Neuza Maria;Mendon?a, Rita Maria Zucatelli;Woe Fang, Francisco Liauw;Ghiuro Valentini, Elizabeth Juliana;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1992, DOI: 10.1590/S0036-46651992000100005
Abstract: ten lots of fuenzalida & palacios type antirabies vaccine for human use, produced at the instituto butantan (s?o paulo, brazil) were stored at temperatures of 45, 37, 28 and 2-8oc. the potency of each lot was determined in samples taken at varied time intervals using the nih method and lots presenting antigenic values > 0,3 were considered satisfactory for use. after 2 hours at 45oc the antigenic value of one out of 10 lots tested was found to be less than the minimum required value. at 37oc all lots maintained satisfactory antigenic values until the third day of storage, whilst at 28 and 2-8oc the potency was fully maintained, respectivelly for 10 and 360 days. at the ideal temperature of 2-8oc, 100% of the tested vaccines maintained the minimum required antigenicity for a longer period (16 months) than the expiry time of 6-12 months usually recommended for this type of biological produced in latin american and caribbean countries. thus, the obtained data suggested that in countries still producing fuenzalida & palacios type vaccine, the expiry time could be extended to 16 months, what could prevent the unnecessary discarding of products still in useful condition.
Efeito do congelamento sobre a imunogenicidade da vacina contra a raiva produzida em tecido cerebral de camundongo
Albas Avelino,Nogueira Rosa Maria Barilli,Fontolan Otávio Luís,Albas Kleber da Silva
Revista da Sociedade Brasileira de Medicina Tropical , 2001,
Abstract: A resposta imune humoral induzida pela vacina contra a raiva produzida em cérebros de camundongos recém-nascidos foi estudada em 23 c es e o teste de soroneutraliza o em camundongos foi usado para avalia o dos níveis de anticorpos rábicos. Um grupo com 10 animais recebeu vacina conservada de 2 a 8°C e apresentou os seguintes resultados: após 30 dias da vacina o 6 (60%) amostras responderam ao teste; após 180 dias 4 (40%) e após 360 dias apenas 1 (10%). O outro grupo com 13 c es recebeu vacina previamente congelada e somente 2 (15,4%) amostras no dia 30 apresentaram resposta satisfatória; os demais períodos (180 e 360) após a vacina o, n o foi encontrado título. A análise estatística dos dados referentes a cada uma das variáveis consideradas no estudo foi efetuada segundo a técnica de análise de variancia seguida por Tuckey e indicaram diferen as estatisticamente significativas entre os grupos.
Efeito do congelamento sobre a imunogenicidade da vacina contra a raiva produzida em tecido cerebral de camundongo
Albas, Avelino;Nogueira, Rosa Maria Barilli;Fontolan, Otávio Luís;Albas, Kleber da Silva;Bremer Neto, Hermann;
Revista da Sociedade Brasileira de Medicina Tropical , 2001, DOI: 10.1590/S0037-86822001000100008
Abstract: the immune humoral response induced by the rabies vaccine produced in suckling mouse brain was studied in 23 dogs. the mouse neutralization test (mnt) was used to evaluate the level of rabies antibodies. ten dogs received vaccine stored at 2 to 8°c, showing the following results: 30 days after vaccination, six samples (60%) responded to the mnt; 180 days after vaccination, 4 samples (40%); and 360 days after vaccination, only one sample (10%). the remaining 13 dogs received previously frozen vaccine and 30 days after vaccination, only two samples (l5.4%) responded to the mnt. no titers were detected 180 and 360 days after vaccination. statistical analysis of each variable used tuckey analysis of variance, which showed statistically significant differences between the two groups.
Diagnóstico laboratorial da raiva na regi?o oeste do Estado de S?o Paulo
Albas, Avelino;Zoccolaro, Paulo Tadeu;Rosa, Talita Zacarias;Cunha, Elenice Maria Sequetin;
Revista da Sociedade Brasileira de Medicina Tropical , 2005, DOI: 10.1590/S0037-86822005000600009
Abstract: the pólo da alta sorocabana laboratory, presidente prudente, sp, brazil, and the biological institute in s?o paulo state, performed an evaluation of rabies diagnosis from 1996 to 2003 in the west region of s?o paulo state. for the tests, the laboratories used direct immunofluorescence and mice inoculation in 4,950 samples, that were sent for analysis involving dogs, cats, cattle, chiroptera (bats) and other animals. according to the results, the laboratories found 74 positive samples; of which 58 (78.4%)were non-hematophagous bats and 16 (21.6%) related to cattle. the present epidemiological study verified that in spite of the high positive index in chiroptera compared to the other species, there was not an outbreak of rabies in the species in the region of presidente prudente, from 1996 to 2003 but a rise in the positive index due to a marked increase in the number of chiroptera samples sent to the laboratories for virus rabies research.
Imunofluorescência realizada em cérebros de camundongos infectados com vírus rábico - cepa CVS, em diferentes estágios de decomposi??o
Valentini, Elizabeth Juliana Ghiuro;Albas, Avelino;Augusto, Vera Lúcia Mendes;Ito, Fumio Honma;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1991, DOI: 10.1590/S0036-46651991000300003
Abstract: the efficiency of the fluorescent antibody (fa) test in detecting rabies virus antigen in decomposed specimens was evaluated in simulated conditions of the safety test recommended for the assessment of residual virus in inactivated rabies vaccines. the cvs-infected mice were submitted to different treatments combining time and temperature in order to cause different stages of carcass decomposition and, the fa test was carried out sequentially at pre-determined time intervals. for the materials stored at 25 oc, greater difficulties for prompt recognition of the inclusion bodies were found after 12 - 18h, whilst the specimens maintained at 4oc, the inclusions were easily visualized for up to 48h. brain smears of carcasses kept at -20oc were suitable for adequate identification after 720 h of storage. in carcasses that had been maintained at 25oc for 10 h with additional storage at 4 or -20oc, rabies antigenicity could not be detected, respectively after 10 and 24 h, due to tissue decomposition. the authors recommend that the fa test, when applied as an additional tool for the control of the safety test of inactivated rabies vaccine using mice, care must be taken in order to avoid the use of decomposed materials.
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