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Search Results: 1 - 10 of 462029 matches for " A Haghnazari "
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Genetic diversity among varieties and wild species accessions of pea (Pisum sativum L.) based on SSR markers
J Nasiri, A Haghnazari, J Saba
African Journal of Biotechnology , 2009,
Abstract: To assess the genetic relations inPisum genus and to examine putative duplicate accessions, 20 pea varieties (Pisum sativum L.) with 57 accessions from wild Pisum species fulvum, subspecies (subsp.) asiaticum, elatius, thebaicum, abyssinicum, transcaucasicum and arvense were analyzed using 10 out of 20 microsatellite primer pairs. We genotyped all accessions. In total, 59 alleles were identified in whole collection. The maximum number of alleles (8 alleles) was obtained from the PEACPLHPP, AF004843, and AA43090 loci. The maximum number of private alleles (4) in the wild collection was detected in AF004843 locus but in the cultivar collection, it was detected in AA430902 and PSBLOX13.2 loci. Cluster analysis and principal coordinate analysis located accessions in 3 groups and cultivated varieties were obviously separated from the wild accessions. Analysis of molecular variance (AMOVA) revealed that the intergroups component of variance (29%) is lower than the intragroups component of variance (71%). The lowest value of genetic differentiation ( Pisum genus and to examine putative duplicate accessions, 20 pea varieties (Pisum sativum L.) with 57 accessions from wild Pisum species fulvum, subspecies (subsp.) asiaticum, elatius, thebaicum, abyssinicum, transcaucasicum and arvense were analyzed using 10 out of 20 microsatellite primer pairs. We genotyped all accessions. In total, 59 alleles were identified in whole collection. The maximum number of alleles (8 alleles) was obtained from the PEACPLHPP, AF004843, and AA43090 loci. The maximum number of private alleles (4) in the wild collection was detected in AF004843 locus but in the cultivar collection, it was detected in AA430902 and PSBLOX13.2 loci. Cluster analysis and principal coordinate analysis located accessions in 3 groups and cultivated varieties were obviously separated from the wild accessions. Analysis of molecular variance (AMOVA) revealed that the intergroups component of variance (29%) is lower than the intragroups component of variance (71%). The lowest value of genetic differentiation (Pisum genus and to examine putative duplicate accessions, 20 pea varieties (Pisum sativum L.) with 57 accessions from wild Pisum species fulvum, subspecies (subsp.) asiaticum, elatius, thebaicum, abyssinicum, transcaucasicum and arvense were analyzed using 10 out of 20 microsatellite primer pairs. We genotyped all accessions. In total, 59 alleles were identified in whole collection. The maximum number of alleles (8 alleles) was obtained from the PEACPLHPP, AF004843, and AA43090 loci. The maximum number of private alleles (4) in the wild collection was detected in AF004843 locus but in the cultivar collection, it was detected in AA430902 and PSBLOX13.2 loci. Cluster analysis and principal coordinate analysis located accessions in 3 groups and cultivated varieties were obviously separated from the wild accessions. Analysis of molecular variance (AMOVA) revealed that the intergroups component of variance
Tagging of resistance gene(s) to rhizomania disease in sugar beet (Beta vulgaris L.)
A Nouhi, R Amiri, A Haghnazari, J Saba, M Mesbah
African Journal of Biotechnology , 2008,
Abstract: The rhizomania disease is one of the most important diseases in Iran and some other parts of the world which potentially could play a role in decreasing sugar yield in fields. One approach to combat with this disease is the use of resistance varieties. This varieties have been identified which are having resistance genes to rhizomania disease (i.e. Rz1, Rz2). In order to use these genes in breeding programs (MAS) tagging these genes with molecular markers is necessary. In our study, we used infected soil which was provided from infected fields then greenhouse test was done to identify resistance and susceptible plants. Extracted DNA from leaves of resistant and susceptible plants was bulked to provide two bulks for resistance and susceptible plants. Three-hundred RAPD primers were used in analysis of the two bulks and two F2 populations. One population was obtained from a cross between Holly1-4 as resistance parent and an annual variety as susceptible plant. The second population was constructed by crossing between WB42 as resistance parent and L 261 as susceptible one. Finally genes were tagged using two RAPD primers and one of the markers is OP-091150 which is 27 cM apart from Rz1 gene in coupling phase. The second marker is OP-AN9600 which is 13.7 cM apart from Rz1 gene and in repulsion phase.
Evaluation of Relationship between Different Traits in Lentils (Lens culinaris Medik)
M. Salehi,A. Haghnazari,F. Shekari,H. Baleseni
Journal of Science and Technology of Agriculture and Natural Resources , 2007,
Abstract: In order to evaluate relationship between Different traits in lentils (lens culinaris Medik), a field study was conducted as an RCBD based design with 3 replications on Zanjan University Research Farm during spring of the year 2004. ANOVA analysis revealed significant differences for all characters except for the number of primery branches. Correlation analysis indicated positive and significant correlation between seed yield and harvest index, number of primery branches, pods/plant and biological yield, and grian yield. The result of the factor analysis also showed that the second factor including number of primery branches, pods/plant, grian yield, canopy width and seeds/plant was an important trait involved in the grian yield in lentil. In addition, cluster analysis helped divide the genotypes into four distance groups of large, medium, semi medium and low yields.
THE BLOOD LEVELS OF GLUCAGON, CORTISOL, AND INSULIN FOLLOWING THE INJECTION OF VENOM BY THE SCORPION (Mesobuthus tamulus concanesis, POCOCK) IN DOGS
RADHA KRISHNA MURTHY, K.;HAGHNAZARI, L.;
Journal of Venomous Animals and Toxins , 1999, DOI: 10.1590/S0104-79301999000100004
Abstract: severe envenoming was induced in two groups of experimental dogs after subcutaneous (sq) injection of venom of the scorpion (mesobuthus tamulus concanesis, pocock) (3.0 and 3.5 mg/kg body weight). the circulating levels of blood sugar, insulin, glucagon, and cortisol were assayed at 0, and 30, 60, 90 and 120 min after venom injection. there was an increase in the circulating levels of blood sugar, insulin, glucagon, and cortisol following envenoming. scorpion envenoming causes an autonomic storm resulting in a massive release of catecholamines, angiotensin ii, glucagon, and cortisol accompanied by changes in insulin secretion. the rise in the counter-regulatory hormones (glucagon, cortisol, and catecholamines) oppose the anabolic actions of insulin resulting in a variety of clinical manifestations. these changes may lead to a syndrome of fuel-energy deficits and to an inability of the vital organs to utilise the existing metabolic substrates, ultimately resulting in multisystem organ failure (msof) and death.
THE USE OF SEROTHERAPHY TO REVERSE ECG and CARDIAC ENZYME CHANGES CAUSED BY SCORPION Mesobuthus tamulus concanesis, Pocock ENVENOMING
MURTHY, K. RADHA KRISHNA;ZARE, M. ABBAS;HAGHNAZARI, L.;
Journal of Venomous Animals and Toxins , 1999, DOI: 10.1590/S0104-79301999000200004
Abstract: acute myocardiopathy in experimental dogs and rabbits was induced by subcutaneous (sq) injection of 3.5 mg/kg of scorpion venom from mesobuthus tamulus concanesis, pocock. an increase in circulating lactic dehydrogenase (ldh), serum glutamic oxalacetic transaminase (sgot), creatine kinase-mb isoenzyme (ck-mb), serum glutamine pyrovate transaminase (sgpt) and alpha hydroxy butyrate dehydrogenase (hbdh) enzyme levels was observed in dogs 60 min after venom injection, and a further rise was observed 120 min after venom injection. the administration of the species-specific scorpion antivenom (sav) at different time intervals after venom injection resulted in reversal of electrocardiographic changes and a reduction in cardiac enzyme levels. the administration of sav to scorpion envenomed alloxan-pretreated animals did not cause clinical or biochemical improvement. on the other hand, administering insulin to envenomed only animals or envenomed alloxan-pretreated animals resulted in a biochemical and clinical improvement, as well as in a reduction of the cardiac enzyme levels. insulin administration in scorpion envenoming syndrome is essentially a metabolic support to control the adverse effects triggered by catecholamines and other counter-regulatory hormones.
Investigations on the role of insulin and scorpion antivenom in scorpion envenoming syndrome
Radha Krishna Murthy, K.;As, Dubey;Zare Abbas, M.;Haghnazari, L.;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2003, DOI: 10.1590/S1678-91992003000200006
Abstract: acute myocardiopathy in alloxan treated experimental dogs and rabbits was induced by subcutaneous (sq) injection of scorpion venom from mesobuthus tamulus concanesis, pocock. envenoming resulted in an initial transient hypertension (180-320 mm hg.) followed by hypotension. simultaneous administration of venom and species-specific scorpion antivenom (sav) prevented hypertension and hypotension. hypotension did not occur when sav was given 60 min after envenoming. blood glucose, triglycerides, cholesterol, amylase, insulin, glucagon, cortisol, hematocrit, mean corpuscular volume (mcv), mean corpuscular hemoglobin (mch), mean corpuscular hemoglobin concentration (mchc), platelet count, red blood cell (rbc) count, hemoglobin (hb), 2,3-diphosphoglycerate (2,3-dpg), and glutathione levels were increased 60 and 90 min after envenoming. total white blood cell (wbc) count was reduced 60 min and increased 90 min after envenoming. simultaneous administration of venom and sav did not alter hb, mchc, and packed cell volume (pcv) levels, or ecg, and cardiovascular, biochemical, metabolic, and hormonal changes. hematological parameters were reversed when sav was given 30 and 60 min after envenoming. pcv, hb, and mchc values returned to normal 120 min after sav. alloxan-treated dogs showed increased blood glucose, cholesterol, glucagon, cortisol levels; reduced glycogen content of liver, cardiac and skeletal muscles; and reduced insulin levels and insulin/ glucagon ratio (i/g ratio). envenoming in the alloxan pre-treated dogs further increased these levels and reduced tissue glycogen content, insulin levels, and i/g ratio. administration of 4 u of insulin to alloxan pre-treated envenomed rabbits caused a biochemical and clinical improvement and increased glycogen content of all tissues in comparison with the values from those administered with sav to alloxan pre-treated envenomed animals. sav administration to envenomed alloxan pre-treated rabbits did not cause clinical or biochemi
The Study of Seed Yield and Seed Yield Components of Lentil (Lens culinaris Medik) under Normal and Drought Stress Conditions
Mohammad Salehi,Ali Haghnazari,Fared Shekari,Ali Faramarzi
Pakistan Journal of Biological Sciences , 2008,
Abstract: In this study yield and seed yield components of twenty lentil (Lens culinaris Medik) genotypes were compared in a split plot RCBD based design with 3 replications at the Zanjan University Research Farmland in 2004. The main plots were lentil planted under drought stress and non stress (irrigation) condition and subplots were twenty of genotype lentil. There were significant differences between traits in lentil genotypes. The seed yield per plant was sensitive to drought stress but 100 seed weight was more tolerance and stable trait in drought condition. As correlation analysis of traits in various stress condition, the harvest index, seed yield per plant, pods per plant and biological yield were correlated with grain yield. In addition harvest index, seed yield per plant, pods/plant and biological yield were the most important traits that have a relationship with grain yield.
Micropropagation and medium-term conservation of Rosa pulverulenta = Micropropaga o e conserva o durante médio prazo de Rosa pulverulenta
Somayeh Kavand,Maryam Jafarkhani Kermani,Ali Haghnazari,Pegah Khosravi
Acta Scientiarum : Agronomy , 2011,
Abstract: In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 êM BAP + 0 êM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 êM BAP + 3 êM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 êM IBA + 0.5 êM NAA during the rooting stage. Comparing the refrigerator and phytotronconditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). Furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron. No Ira, um numero grande de especies de Rosa sao expostas a extincao. Por causa disto, astecnicas de preservacao sao necessarias para garantir o futuro d estas mesmas especies. Nesta investigacao, os objetivos foram aperfeicoar a micropropagacao e preservar durante medio prazo uma destas especies, Rosa pulverulenta. No estadio de proliferacao, o numero maximo de novas folhas (9,60) foi produzido no meio contendo 4 êM BAP + 0 êM GA3, mas o numero maximo de gemas axilares foi observado no meio com 4 êM BAP + 3 êM GA3. Os resultados obtidos permitiram inferir que o maior aumento na altura de haste (49,5 mm) durante o estadio de climatizacao foi observado nos explantes tratados com 1 êM IBA + 0,5 êM NAA durante oenraizamento. Observa-se que embora a geladeira tenha proporcionado uma menor taxa de crescimento, a sobrevivencia em medio prazo foi maior (100,00%) do que no phytotron (87,50%). Alem disto, a taxa de crescimento dos explantes que foram mantidos na geladeira foi acelerada durante o periodo de recuperacao e ficaram proximas daquelas mantidas no phytotron.
Nitration of Phenols with Fe(NO3)3.9H2O in the Presence of Nano-SiO2 as an Efficient Catalyst
Nahid Haghnazari,Changiz Karami,Keivan Ghodrati,Fariba Maleki
International Nano Letters , 2011,
Abstract: Selective nitration of phenols with Fe (NO3)3.9H2O was carried out in the presence of nano-SiO2 at room temperature in good to high yields and short reaction times. The reactions were performed in various solvents and the catalyst could be reused for several runs. The use of nano-SiO2 as catalyst for first time is an advantage of this method.
Micropropagation and medium-term conservation of Rosa pulverulenta
Kavand, Somayeh;Kermani, Maryam Jafarkhani;Haghnazari, Ali;Khosravi, Pegah;Azimi, Mohamad Reza;
Acta Scientiarum. Agronomy , 2011, DOI: 10.4025/actasciagron.v33i2.10279
Abstract: in iran, a large number of rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. in the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, rosa pulverulenta. at proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 μm bap + 0 μm ga3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 μm bap + 3 μm ga3. the results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 μm iba + 0.5 μm naa during the rooting stage. comparing the refrigerator and phytotron conditions for medium-term conservation of in vitro plantlets indicated that although the refrigerator conditions resulted in lower growth rate compared with the phytotron, the survival rate in refrigerator (100%) was significantly higher than phytotron (87.5%). furthermore, the growth rate of the plantlets from the refrigerator was accelerated during the recovery period and verged on to the ones stored in the phytotron.
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