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Search Results: 1 - 10 of 3322 matches for " (Bio)Marker "
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Nephrotic Sydrome Can Be a Marker for Prostatic Carcinoma  [PDF]
Natasha Takova, Alexander Otsetov
Modern Research in Inflammation (MRI) , 2017, DOI: 10.4236/mri.2017.64004
Abstract: Paraneoplastic syndromes (PS) represent a large spectrum of symptoms, associated with malignant diseases. PS can be diagnosed in asymptomatic patients with occult carcinoma, clinically active cancer, and during clinical remission, suggesting a recurrence of the neoplasm. The underlying mechanisms of PS are not completely understood but several authors have suggested that the increased production of biologically active immune factors and cytokines from the neoplastic cells may underlie the etiology of PS. Although rare, the renal involvement of patients with prostatic carcinoma has been reported. The most common paraneoplastic-associated glomerulopathy in prostatic cancer is the membranoproliferative glomerulonephritis with nephrotic syndrome (NS). In this review, we aimed to discuss the incidence of nephrotic syndrome secondary to prostatic carcinoma, its challenging diagnosis, clinical manifestation, and treatment.
In Silico Mining of EST-SSRs in Jatropha curcas L. towards Assessing Genetic Polymorphism and Marker Development for Selection of High Oil Yielding Clones  [PDF]
Neeraj Jain, Ganesh B. Patil, Poonam Bhargava, Rajani S. Nadgauda
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.511167
Abstract:



In recent years, Jatropha curcas L. has gained popularity as a potential biodiesel plant. The varying oil content, reported between accessions belonging to different agroclimatic zones, has necessitated the assessment of the existing genetic variability to generate reliable molecular markers for selection of high oil yielding variety. EST derived SSR markers are more useful than genomic markers as they represent the transcriptome, thus, directly linked to functional genes. The present report describes the in silico mining of the microsatellites (SSRs) using J. curcas ESTs from various tissues viz. embryo, root, leaf and seed available in the public domain of NCBI. A total of 13,513 ESTs were downloaded. From these ESTs, 7552 unigenes were obtained and 395 SSRs were generated from 377 SSR-ESTs. These EST-SSRs can be used as potential microsatellite markers for diversity analysis, MAS etc. Since the Jatropha genes carrying SSRs have been identified in this study, thus, EST-SSRs directly linked to genes will be useful for developing trait linked markers.



RAPD-SCAR Markers: An Interface Tool for Authentication of Traits  [PDF]
Sameer S. Bhagyawant
Journal of Biosciences and Medicines (JBM) , 2016, DOI: 10.4236/jbm.2016.41001
Abstract: The versatility of the PCR technique is that several kinds of primers can be explored for genome analysis depending on the purpose of study. The easy to access and low cost PCR-based markers include Random Amplified Polymorphic DNA (RAPD). The RAPD markers are easy to develop but lack of reproducibility makes it less reliable and obstacles to their further use in authentication of traits. In addition, other PCR and non PCR based markers like Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeat (SSR) and Restriction Fragment Length Polymorphism (RFLP) are also employed in authentication of traits with certain restrictions vis-à-vis use of radioactive materials, high cost and requirement of sequence information etc. Therefore, this problem can be overcome by converting RAPD markers to more robust sequence characterized amplified regions i.e. SCAR markers. SCARs are locus specific, co-dominant in nature and amplified by PCR using specific 15 - 30 bp DNA fragments. For developing SCAR markers, primers are designed from the nucleotide sequences of a cloned RAPD fragments linked to a trait of interest. SCAR markers are easy to develop and reliable tools for DNA fingerprinting. This mini review is an attempt to summarize efficacy of RAPD-SCAR interface in authentication of traits.
Bio-Organism Damage under the Influence of Microwave Heating  [PDF]
Ziwei Xie
Journal of Biosciences and Medicines (JBM) , 2019, DOI: 10.4236/jbm.2019.74005
Abstract: In this paper, we setup a simple model to understand the damage of bio-organism under the influence of microwave heating based on the bio-heat transfer equation and Arrhenius equation. Detailed information of temperature distribution and fraction of damage is presented. Results show that microwave heating is effective in the damage of bio-organism.
Applying Molecular Breeding to Improve Soybean Rust Resistance in Vietnamese Elite Soybean  [PDF]
Tran Dang Khanh, Truong Quoc Anh, Tran Dang Xuan, Bui Chi Buu
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.41001
Abstract: Soybean production in Vietnam has recently been threatened by the widespread dissemination of soybean rust (SBR) caused by the fungus Phakopsora pachyrhizi. Application of molecular breeding is considered as a feasible method to improve soybean rust resistance and minimize the adverse effects from overuse fungicides in this country. In this study, we have successfully applied molecular markers in a backcross breeding program to introgress the Rpp5 gene of SBR resistance into HL203, an elite Vietnamese soybean variety, from two donor lines of DT2000 and Stuart 99084B-28. The plants in BC4F1 generation had maximum contribution from the recurrent parents and retained SBR resistance gene.
Characterization of seed storage protein patterns of four Iranian Pistachios using SDS-PAGE  [PDF]
Ali Akbar Ehsanpour, Behrokh Shojaie, Fatemeh Rostami
Natural Science (NS) , 2010, DOI: 10.4236/ns.2010.27092
Abstract: We used SDSPAGE to evaluate and characterize the protein patterns of seed storage proteins in four pistachios cultivars (Akbari, Ahmad Aghaei, Fandoghi, and Kaleghouchi). Total protein content of pistachio seeds in all cultivars did not show any significant difference. Results of SDS PAGE pattern of a few protein bands were up regulated whereas some other bands showed down regulation. The identified protein patterns may be used protein marker for pistachio cultivars.
Immunohistochemical Expression of p53 and p63 in Adenomas and Carcinomas of Canine Mammary Glands  [PDF]
Erika M. Terra, Marcela M. M. P. Rodrigues, Geórgia M. Magalhaes, Mirela T. Costa, Renée L. Amorim, Noeme S. Rocha
Open Journal of Veterinary Medicine (OJVM) , 2012, DOI: 10.4236/ojvm.2012.21004
Abstract: Mammary tumors are very common in female dogs and its prognosis varies significantly according to the tumor invasion degree. Myoepithelial layer destruction is considered a differential diagnosis parameter of in situ from invasive lesions in human breast cancer. Aiming to evaluate the immunohistochemical expression of p63, a protein specifically expressed by myoepithelial cells of mammary gland, and its correlation with p53 expression, 10 adenomas and 20 carcinomas with and without metastasis of canine mammary gland were studied. There was no significant difference between adenomas and carcinomas for both proteins staining, however, the percentage and continuity of staining was lower in metastatic carcinomas. There was no correlation between p53 and p63 expression, but the higher values of p53 staining were found in carcinomas, according to previous studies. Results showed that p63 is specifically expressed also in myoepithelial cells of canine mammary gland. However, in order to ensure its use as a prognostic marker in canine mammary tumors, further studies involving a higher number of samples should be conducted.
Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum  [PDF]
Pritesh Parmar, Ankit Sudhir, R. Preethi, Bhaumik Dave, Ketankumar Panchal, Ramalingam B. Subramanian, Arvind Patel, K. B. Kathiria
American Journal of Molecular Biology (AJMB) , 2013, DOI: 10.4236/ajmb.2013.34031
Abstract: With the discovery of molecular markers and marker assisted selection technology, the research has entered into a new era and has made it possible to develop new and more informative PCR-based markers, including SSR, and to further facilitate the use of markers in tomato breeding. The present study is a step to introduce a new SSR marker (TOM-144) which was deduced after evaluation of eight microsatellite loci amongst the twenty-one different tomato cultivars. The marker selected was inherited and segregated in mendelian fashion as demonstrated in successive generation of a cross between parent cvs. H-24 x GT-2.
Radiation Field Preference for Radiographic Anatomical Markers by Radiographers in a University Teaching Hospital in Nigeria  [PDF]
Thomas Adejoh, Sobechukwu Warric Iwene Onwuzu, Flavious Bobuin Nkubli, Nwamaka Ikegwuonu
Open Journal of Radiology (OJRad) , 2014, DOI: 10.4236/ojrad.2014.43036
Abstract: Objective: The work aims to determine the radiographers’ preference between primary and secondary radiation fields for imprinting anatomical markers on radiographs. Methodology: Processed radiographs from the darkroom with evidence of radiographic anatomical markings were selected randomly and reviewed using a viewing box, within a 4-week period. The radiation field in which markers were placed was noted for each radiograph. Faintly-appearing and partly coned- off markers were excluded. Simple statistical tools were used to derive central tendency. Result: 623 radiographs were assessed. 89.0% (n = 555) had markers in the primary radiation field while 11.0% (n = 68) were in the secondary radiation field. 98% (n = 611) of markers did not obstruct essential anatomy while 2% (n = 12) did, but the radiographs were neither repeated nor rejected because of the twin reason of reportability and the need to avoid additional radiation dose to patients. Conclusion: Radiographers in the centre preferred the primary radiation field for marker placement to avoid cone-off, cut-off and illegibility which leads to repeat. This, however, does not offer superior advantage to markers placed in secondary radiation field. It is recommended that marker placement preference should be guided by the need for legibility, aesthetics and avoidance of essential anatomy.
AFLP-SSCP: A Useful AFLP-Based Method for Informative SNPs Discovery in Non-Model Organisms  [PDF]
Te-Hua Hsu, Yue Ning, Jin-Chywan Gwo
Advances in Biological Chemistry (ABC) , 2014, DOI: 10.4236/abc.2014.46042
Abstract: Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among individuals of a species. Recently, in spite of the development of high-throughput genotyping technologies, SNPs have been applied as markers for population genetic and high-density genetic mapping. However, the high costs of SNPs discovery and genotyping assay limit the applications of SNP markers in non-model organisms. In this study, we present a cheap and convenient AFLP-based (Amplified fragment length polymorphism) strategy that is highly efficient for developing informative SNP markers without any prior information. We developed SNP markers in a non-model and economic aquaculture species Asian Seabass (Lates calcarifer), and discussed the potential use of the combinations of AFLP and AFLP-SSCP.
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