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Search Results: 1 - 10 of 11773 matches for " ?ngela Kaysel;Machado "
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A novel A2 allele found in Leishmania (Leishmania) infantum chagasi
Oliveira, Trícia Maria Ferreira de Sousa;Vasconcelos, Elton José Rosas de;Nakaghi, Andréa Cristina Higa;Defina, Tania Paula Aquino;Jusi, Márcia Mariza Gomes;Baldani, Cristiane Divan;Cruz,ngela Kaysel;Machado, Rosangela Zacarias;
Revista Brasileira de Parasitologia Veterinária , 2011, DOI: 10.1590/S1984-29612011000100009
Abstract: visceral leishmaniasis (vl) is a widely spread zoonotic disease. in brazil the disease is caused by leishmania (leishmania) infantum chagasi. peridomestic sandflies acquire the etiological agent by feeding on blood of infected reservoir animals, such as dogs or wildlife. the disease is endemic in brazil and epidemic foci have been reported in densely populated cities all over the country. many clinical features of leishmania infection are related to the host-parasite relationship, and many candidate virulence factors in parasites that cause vl have been studied such as a2 genes. the a2 gene was first isolated in 1994 and then in 2005 three new alleles were described in leishmania (leishmania) infantum. in the present study we amplified by polymerase chain reaction (pcr) and sequenced the a2 gene from the genome of a clonal population of l. (l.) infantum chagasi vl parasites. the l. (l.) infantum chagasi a2 gene was amplified, cloned, and sequenced in. the amplified fragment showed approximately 90% similarity with another a2 allele amplified in leishmania (leishmania) donovani and in l.(l.) infantum described in literature. however, nucleotide translation shows differences in protein amino acid sequence, which may be essential to determine the variability of a2 genes in the species of the l. (l.) donovani complex and represents an additional tool to help understanding the role this gene family may have in establishing virulence and immunity in visceral leishmaniasis. this knowledge is important for the development of more accurate diagnostic tests and effective tools for disease control.
A sobrevivência literária de Francisco Lobo da Costa
Sapper,ngela Machado Treptow
Letras de Hoje , 2007,
Abstract: N o possui resumo
A experiência de aprendizagem on-line em um curso de qualifica??o profissional em saúde
Laguardia, Josué;Casanova,ngela;Machado, Rejane;
Trabalho, Educa??o e Saúde , 2010, DOI: 10.1590/S1981-77462010000100006
Abstract: this article examines the perceptions regarding tutor and student attitudes in the learning processes during an on-line professional refresher course. in the study, interviews were carried out among ten students and three tutors of the course by means of semi-structured roadmaps that covered the following aspects: admission to the course, technologies, content, assessment activities, mentoring, mediation, and expectations about the course. the roadmaps were organized into topics that would enable the identification of the structural conditions for the use of the tools that were available in the virtual environment, the types of interaction, the understanding of the content, as well as participants' expectations with respect to the objectives proposed by the course. the interviewees' reports point to important questions to be considered when designing and managing on-line courses, such as the need for periodic adjustments of the course's educational proposal in order to meet the demands of both students and tutors with regard not only to how the content is organized but also to updating it, the means through which the material can be accessed, and the implementation of assessment activities that are consistent with work experience.
Root phosphatase activity, plant growth and phosphorus accumulation of maize genotypes
Machado, Cynthia Torres de Toledo;Furlani,ngela Maria Cangiani;
Scientia Agricola , 2004, DOI: 10.1590/S0103-90162004000200015
Abstract: the activity of the enzyme phosphatase (p-ase) is a physiological characteristic related to plant efficiency in relation to p acquisition and utilization, and is genetically variable. as part of a study on maize genotype characterization in relation to phosphorus (p) uptake and utilization efficiency, two experiments were set up to measure phosphatase (p-ase) activity in intact roots of six local and improved maize varieties and two sub-populations. plants were grown at one p level in nutrient solution (4 mg l-1) and the p-ase activity assay was run using 17-day-old plants for varieties and 24-day-old plants for subpopulations. shoot and root dry matter yields and p concentrations and contents in plant parts were determined, as well as p-efficiency indexes. root p-ase activity differed among varieties, and highest enzimatic activities were observed in two local varieties -'catet?o' and 'caiano' -and three improved varieties -'sol da manh?', 'nitrodente' and 'br 106'. 'carioca', a local variety, had the lowest activity. between subpopulations, 'nd2', with low yielding and poorly p-efficient plants, presented higher root p-ase activity as compared to 'nd10', high yielding and highly p-efficient plants. in general, subpopulations presented lower p-ase activities as compared to varieties. positive and/or negative correlations were obtained between p-ase activity and p-efficiency characteristics, specific for the genotypes, not allowing inference on a general and clear association between root-secreted phosphatase and dry matter production or p acquisition. genotypic variability must be known and considered before using p-ase activity as an indicator of p nutritional status, or p tolerance, adaptation and efficiency under low p conditions.
Kinetics of phosphorus uptake and root morphology of local and improved varieties of maize
Machado, Cynthia Torres de Toledo;Furlani,ngela Maria Cangiani;
Scientia Agricola , 2004, DOI: 10.1590/S0103-90162004000100012
Abstract: interspecific and intraspecific differences in the efficiency of utilization of soil phosphorus (p) are explained, in part, by plant morphological and physiological variations, which characterize the plant for nutrient acquisition. as part of a study on maize genotypes characterization for p-uptake and use efficiency, kinetic parameters of p uptake and root morphological characters were evaluated for six local and improved maize varieties, grown in nutrient solution. the kinetic parameters related to p influx were: maximal transport rate (vmax), the michaelis-menten constant (km), and the external concentration when net uptake is zero (cmin). measured root morphological characters were: root length, radius, and surface area. shoot and root dry matter yield and the p content in these parts were also determined. diferences among varieties were observed in the kinetic parameters vmax and km, root length and in root/shoot dry matter ratio. lower km values were better indicatives of p uptake ability of the varietes and were significantly correlated to higher dry matter production and p-efficiency index.
Desinfec o de efluente secundário do sistema Uasb + Wetland
Lourdes Teresinha Kist,ênio Leandro Machado,ngela Martins Louren?o
Tecno-Lógica , 2011,
Abstract: Este trabalho avaliou a aplicabilidade do processo UV/TiO2/O3 em reator tipo coluna para desinfec o de efluentes secundários da ETE piloto UASB + Wetland da UNISC. O reator foi testado de 0 a 60 minutos, alimentado com os efluentes secundários estudados em pH=7,0. Os ensaios foram realizados com tempo de recircula o de 1 hora, vaz o de 180 L h –1 e lampadas germicidas de 30 W. Os resultados obtidos demonstraram a aplicabilidade do reator tipo coluna para desinfec o de esgotos tratados, sendo obtida desinfec o total em tempos de deten o hidráulica de 12 minutos, a partir das inativa es de coliformes termotolerantes. Também, os experimentos utilizando o processo UV/TiO2/O3 demonstraram maior capacidade de oxida o em fun o das redu es da alcalinidade total e turbidez, indicando maiores potencialidades para a desinfec o. Abstract This work assessed the applicability of UV/TiO2/O3 processes in photo reactors column type for disinfection of secondary effluents of ETE pilot UASB + Wetland of UNISC. The reactors were tested with the same detention times of 0 to 60 minutes, both fed with the studied secondary effluents in pH=7. The tests were carried out with a recirculation time of 1 hour, flow of 180 L h-1, germicide lamps of 30W. The obtained results showed the applicability of the reactors type column for disinfection of treated sewage, obtained total disinfection in HDT’s of 12 minutes, from the inactivation of thermo-tolerant coliforms. Also, the experiments using the UV/TiO2/O3 process had demonstrated to greater capacity of oxidation in function of the reductions of total alkalinity and turbidity, indicating major potentialities for the disinfection.
Root phosphatase activity, plant growth and phosphorus accumulation of maize genotypes
Machado Cynthia Torres de Toledo,Furlani ?ngela Maria Cangiani
Scientia Agricola , 2004,
Abstract: The activity of the enzyme phosphatase (P-ase) is a physiological characteristic related to plant efficiency in relation to P acquisition and utilization, and is genetically variable. As part of a study on maize genotype characterization in relation to phosphorus (P) uptake and utilization efficiency, two experiments were set up to measure phosphatase (P-ase) activity in intact roots of six local and improved maize varieties and two sub-populations. Plants were grown at one P level in nutrient solution (4 mg L-1) and the P-ase activity assay was run using 17-day-old plants for varieties and 24-day-old plants for subpopulations. Shoot and root dry matter yields and P concentrations and contents in plant parts were determined, as well as P-efficiency indexes. Root P-ase activity differed among varieties, and highest enzimatic activities were observed in two local varieties -'Catet o' and 'Caiano' -and three improved varieties -'Sol da Manh ', 'Nitrodente' and 'BR 106'. 'Carioca', a local variety, had the lowest activity. Between subpopulations, 'ND2', with low yielding and poorly P-efficient plants, presented higher root P-ase activity as compared to 'ND10', high yielding and highly P-efficient plants. In general, subpopulations presented lower P-ase activities as compared to varieties. Positive and/or negative correlations were obtained between P-ase activity and P-efficiency characteristics, specific for the genotypes, not allowing inference on a general and clear association between root-secreted phosphatase and dry matter production or P acquisition. Genotypic variability must be known and considered before using P-ase activity as an indicator of P nutritional status, or P tolerance, adaptation and efficiency under low P conditions.
Kinetics of phosphorus uptake and root morphology of local and improved varieties of maize
Machado Cynthia Torres de Toledo,Furlani ?ngela Maria Cangiani
Scientia Agricola , 2004,
Abstract: Interspecific and intraspecific differences in the efficiency of utilization of soil phosphorus (P) are explained, in part, by plant morphological and physiological variations, which characterize the plant for nutrient acquisition. As part of a study on maize genotypes characterization for P-uptake and use efficiency, kinetic parameters of P uptake and root morphological characters were evaluated for six local and improved maize varieties, grown in nutrient solution. The kinetic parameters related to P influx were: maximal transport rate (Vmax), the Michaelis-Menten constant (Km), and the external concentration when net uptake is zero (Cmin). Measured root morphological characters were: root length, radius, and surface area. Shoot and root dry matter yield and the P content in these parts were also determined. Diferences among varieties were observed in the kinetic parameters Vmax and Km, root length and in root/shoot dry matter ratio. Lower Km values were better indicatives of P uptake ability of the varietes and were significantly correlated to higher dry matter production and P-efficiency index.
Infec??o pelo vírus Epstein Barr e hepatite
Moreira,Eunice; Machado,ngela; Machado,Leonilde; Xavier,Célia; Monteiro,Cláudia; Cunha,Joaquim; Garrido,Cristina;
Nascer e Crescer - Revista do Hospital de Crian?as Maria Pia , 2011,
Abstract: introduction:epstein barr virus (veb) infection is very prevalent with more than 90% of adults worldwide seropositive. the majority of primary veb infections are subclinical. acute complications are very rare but can be fatal. case report: a nine-year-old girl was admitted with fever for 13 days, abdominal pain and sore throat. laboratory results were consistent with an acute viral hepatitis with markedly elevated transaminase levels. the diagnosis of acute veb infection was confirmed by the positive heterophilic antibody monospot assay and serological markers. conclusion: although common, liver disease is usually mild with transient elevation of serum aminotransferases, and cases of severe hepatitis are rare. the authors report this case due to the unexpected severity of liver disease.
Ploidy mosaicism and allele-specific gene expression differences in the allopolyploid Squalius alburnoides
Isa Matos, élio Sucena, Miguel P Machado, Rui Gardner,ngela Inácio, Manfred Schartl, Maria M Coelho
BMC Genetics , 2011, DOI: 10.1186/1471-2156-12-101
Abstract: In this work we intended to correlate expression differences between individuals and/or between organs to the occurrence of mosaicism, evaluating if mosaics could explain previous observations and its impact on the assessment of gene expression patterns.To achieve our goal, we developed flow cytometry and cell sorting protocols for this system generating more homogenous cellular and transcriptional samples. With this set-up we detected 10% ploidy mosaicism within the S. alburnoides complex, and determined the allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin) in cells from liver and kidney of mosaic and non-mosaic individuals coming from different rivers over a wide geographic range.Ploidy mosaicism occurs sporadically within the S. alburnoides complex, but in a frequency significantly higher than reported for other organisms. Moreover, we could exclude the influence of this phenomenon on the detection of variable allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin) in cells from liver and kidney of triploid individuals. Finally, we determined that the expression patterns previously detected only in a narrow geographic range is not a local restricted phenomenon but is pervasive in rivers where S. pyrenaicus is sympatric with S. alburnoides.We discuss mechanisms that could lead to the formation of mosaic S. alburnoides and hypothesise about a relaxation of the mechanisms that impose a tight control over mitosis and ploidy control in mixoploids.The chromosome theory of heredity rests on the consistency and stability of chromosome number and composition [1]. This consistency and stability is achieved by the existence of extremely precise and tightly controlled mechanisms of chromosome replication and segregation during cell divisions [2]. However, genetic information and the way it is inherited are not so invariant and rigorous as previously thought [3]. Experimental findings in reproductive genetics h
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