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Search Results: 1 - 10 of 113132 matches for " 朱万龙 "
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云南汉坦病毒宿主的研究
Study of Hantavirus’s Hosts in Yunnan Province
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沐远,
Bioprocess (BP) , 2013, DOI: 10.12677/BP.2013.34006
Abstract: 汉坦病毒主要是由啮齿类动物传播的病原体,它可以引起肾综合征出血热(HFRS)和汉坦病毒肺综合征(HPS),由于其对人的致死率极高,近年来对该病毒的研究也越来越多。与此同时,为了搞清楚该病毒的起源和传播途径,对其宿主的研究也就显得越来越重要。云南啮齿类动物种类丰富,其中很多都可以携带汉坦病毒,本综述主要对云南汉坦病毒宿主的相关问题进行阐述,这些都对于预测和预防汉坦病毒的流行有着重要的意义。
Hantavirus is a mainly rodent-borne pathogen, which can cause hemorrhagic fever with renal syndrome (HFRS) and Hantavirus pulmonary syndrome (HPS). Because of its high mortality rate for people, in recent years more and more studies about the virus were reported. At the same time, in order to clarify the origin and transmission of the virus, research on its host also becomes more and more important. The rodents are abundant in Yunnan province, and many of them are the hosts of Hantavirus. In the present study, we mainly elaborate the distribution and evolution about the Hantavirus’ hosts in Yunnan province, which had great significance for the prediction and prevention of the Hantavirus epidemic.
云南呈贡和剑川地区高山姬鼠消化道形态的比较
Comparative Study of Digestive Tract Morphology of Apodemus chevrieri in Chenggong and Jianchuan of Yunnan Province
 [PDF]

郑佳,
Bioprocess (BP) , 2014, DOI: 10.12677/BP.2014.44009
Abstract:
为探讨不同地区的高山姬鼠(Apodemus chevrieri)消化道形态是否存在差异,本研究于2012年6月分别对捕自云南剑川和呈贡地区高山姬鼠的体重、消化道(胃、小肠、大肠和盲肠)的长度以及含内容物重、去内容物重进行了测量。结果显示:剑川地区高山姬鼠的小肠和盲肠去内容物重以及长度均显著大于呈贡地区;体重、胃和盲肠含内容物重以及盲肠长均显著小于呈贡地区。结果表明:剑川地区的高山姬鼠具有较小的体重以减少对能量的绝对需求来应对该地较低的气温和食物资源短缺,暗示消化道形态的大小和消化能力的调整在自然环境高山姬鼠消化对策中起着重要的作用。
In order to investigate whether there are differences on digestive tract morphology in A. chevrieri from different areas, we measured weight and lengths of total digestive tract morphology including stomach, small intestine, large intestine and cecum of A. chevrieri from Jianchuan and Chenggong. The results showed that the length and weight without contents of the small intestine and cecum in A. chevrieri at Jianchuan were significantly greater than that of Chenggong; body mass, the stomach, the weight with content of stomach and cecal and lengths of cecum at Jianchuan were significantly less than that of Chenggong. The results indicated that A. chevrieri at Jianchuan might adjust the body mass and weight of digestive tract to maintain the normal physiological function in stressed condition, such as at cold environment and lack of food. This suggests that the adjustments in capacity and size of the gastrointestinal tract play an important role in their digestive strategies of A. chevrieri in natural environment.
云南生物多样性现状及保护对策探究
Study of Biodiversity Situation and It’s Conservation Measure in Yunnan Province
 [PDF]

高文荣,
Bioprocess (BP) , 2014, DOI: 10.12677/BP.2014.43005
Abstract:
云南省是我国生物多样性最为丰富的地区,保存有许多珍稀、特有和古老的类群,是公认的生物多样性重要类群分布最为集中、具有全球意义的生物多样性关键地区之一。进行云南生物多样性现状及保护对策的探究,对于我国乃至世界生物多样性的保护与可持续利用都具有重要的理论价值和实践意义。本文阐明了生物多样性的概念、价值及意义,对我国云南的动物资源、植物资源,生态系统类型,物种遗传资源等进行了系统的分析,针对我国云南生物多样性现状,论述了云南生物多样性的丰富性和特有性等特征,进而分析了云南生物多样性现存的问题。在此基础上,提出相应的保护对策,并且提出了关于云南生物多样性的一些展望。
Yunnan province is the most abundant area of biodiversity in China, which saves a lot of rare, en-demic and ancient groups and is one of the most important focuses of global significance and one of the key areas of biological diversity taxa distribution. Exploring the Countermeasures of Yunnan status of biodiversity and protection has the important theory value and practical significance for the protection of our country and the world of biological diversity and sustainable utilization. This paper illustrated the concept, value and significance of biodiversity. Animal resources, plant resources, ecosystem types and genetic of the species resources in Yunnan province were studied systematically. In view of the status of biodiversity in Yunnan, the paper discussed richness, en-demism and other feature of biodiversity and analyzed the existing problems of biodiversity in Yunnan. In the end, the paper pointed out the strategies related to the existing problems and made outlook about the biodiversity in Yunnan province.
中缅树鼩PRDM16、BMP7、PPARα、COX II及PGC-1α基因的扩增研究
Amplification of PRDM16, BMP7, PPARα, COX II and PGC-1α Genes Tupaia belangeri
 [PDF]

章迪, 张浩,
Bioprocess (BP) , 2014, DOI: 10.12677/BP.2014.43007
Abstract:
本研究对中缅树鼩PRDM16、BMP7、PPARα、COX II及PGC-1α基因的部分序列进行扩增和分析,获得中缅树鼩PRDM16、BMP7、PPARα、COX II及PGC-1α片段分别为184 bp、84 bp、358 bp、212 bp、271 bp,推测其分别编码61、27、119、70、87个氨基酸。将PRDM16、BMP7、PPARα、COX II及PGC-1α基因编码氨基酸序列分别与多种哺乳动物对应的氨基酸序列进行同源性比较分析发现氨基酸的同源性较高。以获得的PRDM16、BMP7、PPARα、COX II及PGC-1α基因序列分别构建系统进化树,结果显示中缅树鼩与灵长类动物的亲缘关系较近。
In the present study, partial cDNA sequence of PRDM16, BMP7, PPARα, COX II and PGC-1α genes in Tupaia belangeri were amplificated and analyzed. The obtained partial sequences of PRDM16, BMP7, PPARα, COX II and PGC-1α in T. belangeri were 184 bp, 84 bp, 358 bp, 212 bp and 271 bp, respectively. The coding protein of these sequences included 61 amino acids in PRDM16; 27 amino acids in BMP7; 119 amino acids in PPARα, 70 amino acids in COX II and 87 amino acids in PGC-1α. Comparison of the amino acids of PRDM16, BMP7, PPARα, COX II and PGC-1α with the amino acids in many mammals found that the homology of amino acids was higher. The PRDM16, BMP7, PPARα, COX II and PGC-1α genes were used to reconstruct phylogenetic trees which showed that T. belangeri had the closest relationship relative to primates.
东洋界特有种——中缅树鼩生理生态学研究进展及其展望
Oriental Endemic Species—Progress and Prospect of Studies on Physiological Ecology in Tree Shrews, Tupaia belangeri
 [PDF]

高文荣,
Bioprocess (BP) , 2014, DOI: 10.12677/BP.2014.44011
Abstract:
中缅树鼩为东洋界特有的小型哺乳动物,具有特殊的进化地位,在生物医学上被广泛采用为实验动物。本论文以中缅树鼩(Tupaia belangeri)为研究对象,从个体、组织及分子水平对中缅树鼩的产热特征及能量代谢进行了系统的研究。得到以下成果:首次从生理生态学角度支持了树鼩由南向北扩散的“岛屿起源”假说,温度是限制中缅树鼩继续向北扩散的主要因素;中缅树鼩的产热特征及能量代谢具有季节性和日节律变化,温度和光周期对产热特征及能量代谢具有显著影响。研究成果为东洋界小型哺乳动物的生理生态适应模式与对策提供了理论依据。
Tree shrew (Tupaia belangeri) is a small mammal in Oriental endemic with special evolution status, and it has been widely used in biomedicine as experimental animal. In this paper, we used T. belan-geri as the research object, thermogenesis and energy metabolism were measured in system of T. belangeri from individual, organization and molecular levels. The following results were obtained: for the first time to support the “island origin” hypothesis that T. belangeri diffused from south to north from the physiological ecology angle, and temperature is the major factor which limited T. be-langeri continued northward diffusion; thermal characteristics and energy metabolism in T. belan-geri with seasonal and diurnal variation; temperature and photoperiod had significant effects on its thermal characteristics and energy metabolism. Research results provide a theoretical basis of phy-siological ecological adaptation model and countermeasures for Oriental small mammals.
中缅树鼩解偶联蛋白-1基因部分序列的扩增与分析
Amplification and Analysis of the Partial cDNA Sequence of UCP1 Gene in Tupaia belangeri
 [PDF]

高文荣,
Bioprocess (BP) , 2014, DOI: 10.12677/BP.2014.44010
Abstract:
解偶联蛋白-1 (UCP1)是分布于线粒体内膜上的一种产热蛋白,其主要功能是参与褐色脂肪组织(BAT)的产热调节和能量代谢来维持机体的能量代谢平衡。本研究通过设计简并引物进行反转录聚合酶链式反应(RT-PCR),从中缅树鼩(Tupaia belangeri)BAT中成功获得444 bp UCP1 cDNA核心片段。推测其氨基酸序列为148个氨基酸;经结构功能分析发现,此段氨基酸序列具有32 bp UCPs 共同具有的同源序列,具有13 bp中缅树鼩BAT UCP1的特异性序列;进行氨基酸同源性序列比较分析表明,中缅树鼩与小家鼠、褐家鼠、灰仓鼠、家兔、马、猫、牛、海象、黑猩猩和大熊猫UCP1的同源性分别为80%、81%、82%、86%、85%、86%、89%、87%、83%和88%。结果表明:UCP1编码区在哺乳动物中具有较高保守性,提示UCP1在脊椎动物中扮演着重要的生理角色。
Uncoupling protein-1 (UCP1) is a kind of heat producing protein, which distributes on the inner membrane of mitochondria. Its main function is to maintain the body’s energy metabolic balance by participating thermogenic regulation and energy metabolism in BAT. In this study, we designed primers and used reversing transcriptase polymerase chain reaction (RT-PCR). This experiment successfully obtained 444 bp BAT UCP1 cDNA core fragment of tree shrews, and we speculated that the amino acid sequence were 148 amino acids. After structure-function analysis, we found that fragment composed of these amino acid possessed 32 bp homologous sequence of UCPs and 13 bp specific sequence of UCP1 in Tupaia belangeri. After the comparison of amino acid sequence ho-mology, the results showed that the UCP1 homology between tree shrews and other animals like Mus musculus, Rattus norvegicus, Cricetulus griseus, Oryctolagus cuniculus, Equus caballus, Felis ca-tus, Bos taurus, Odobenus rosmarus, Pongo abelii and Ailuropoda melanoleuca was respectively 80%, 81%, 82%, 86%, 85%, 86%, 89%, 87%, 83% and 88%. UCP1 coding region showed higher conser-vation in mammals, suggesting that UCP1 plays an important physiological role in vertebrates.
屏边大围山自然保护区倭蜂猴(Nycticebus pygmaeus)栖息地植被特征的研究
Study on the Vegetation Types of the Nycticebus pygmaeus Habitats at Daweishan Nature Reserve in Pingbian
 [PDF]

高文荣, 蔡金红,
Bioprocess (BP) , 2014, DOI: 10.12677/BP.2014.43006
Abstract:
2009年2月6日~2月27日、2009年10月1日~10月24日和2010年4月20日~25日采用社区访谈和野外实地调查方法,共研究了10个地区,了解倭蜂猴在屏边大围山保护区及其周边社区的栖息地植被及数量状况。结果表明:取食生境中植被主要以漆树科,蔷薇科和壳斗科植物为主。倭蜂猴分布栖息地植被主要以杜英科,壳斗科和桑科植物为主。此外,倭蜂猴在鸡窝,石头寨,干田,田心,喏咪,火山,马尾冲,独家和碧马邑9个自然村周围存在分布点。其中,通过夜间的样线调查,在独家自然村村有林发现2只倭蜂猴实体。
On the basis of community interviews and wild quantity investigation in the night to Nycticebus pygmaeuses, we surveyed the current distribution and quantity situation of N. pygmaeus at the Daweishan Nature Reserves in Pingbian during the following three periods: February 6-27, 2009, October 1-24, 2009 and April 20-25, 2010, and the total 10 areas were study. The results showed that Anacardiaceae, Rosaceae and Fagaceae were dominant families in the feeding habitat. The dominant vegetations were Elaeocarpaceae, Fagaceae and Moraceae which were dominant families in the distribution area. Moreover, the distribution sites and quantity results suggested that the pygmy lorises were mainly distributed in the lower elevation areas, including Jiwo, Shitouzhai, Gantian, Tianxin, Nuomi, Huoshan, Maweichong, Dujia and Bimayi villages. The quantity was rare and only 2 entities were found personally at Dujia village’ forest.
瘦素研究的最新进展和科学意义
Recent Progress in the Study of Leptin and Its Scientific Significance
 [PDF]

高文荣, 张浩, 姜文秀,
Bioprocess (BP) , 2014, DOI: 10.12677/BP.2014.41001
Abstract: 瘦素主要是由脂肪细胞分泌的一种蛋白质,与机体摄食、能量代谢、脂肪存储等的调控密切相关。目前,瘦素抵抗作为肥胖发生的一个重要危险因素已经得到公认,但具体清晰的作用机制仍未阐明,仅有瘦素受体突变、瘦素转运、瘦素信号抑制、血管内缺陷、转换缺陷等各种假说。全球的研究者正试图以这些假说为基础探讨肥胖的发生机制,进而攻克肥胖。本文对瘦素及其受体的基因表达和分子结构做了简单介绍,分析了瘦素参与的JAK/STATRasMAPK信号通路。重点探讨了瘦素抵抗机制的可能通路,以期对肥胖的瘦素抵抗机制研究有一定的参考意义。
Leptin is a protein mainly secreted by the lipocyte, which is related to the regulation of food intake, energy expenditure and fat storage closely. Currently, the theory that leptin resistance is a primary risk factor for obesity has been accepted widely. But the clear mechanism has not been clarified yet. The occurrence of leptin resistance may be related to some hypotheses, such as leptin receptor mutation, leptin transshipment, leptin signal suppression, vascular defects and conversion defects. The researchers from all over the world are trying to discover the mechanism based on these hypotheses and are expecting to cure the obesity completely. This review 1) briefly introduces the gene expression and the molecular structure of leptin and leptin receptor; 2) analyzes the signal transduction of leptin such as JAK/STAT, Ras and MAPK; 3) summarizes the access of leptin resistance. Maybe the work will have the certain reference value to the obesity research in the mechanisms of leptin resistance.
脂肪组织转化关键因子的研究进展
Research Progress of Key Factors for the Transformation of Adipose Tissue
 [PDF]

, 陈金龙, 王政昆
QianRen Biology (QRB) , 2015, DOI: 10.12677/QRB.2015.22002
Abstract:
脂肪组织科分为褐色脂肪组织(Brown adipose tissue, BAT)和白色脂肪组织(white adipose tissue)。BAT为一种特化的产热组织,是小型哺乳动物非颤抖性产热的主要部位。PRDM16 (PR domain-con- taining 16)锌指蛋白在BAT中特异性表达,可触发BAT细胞中PGC-1α (Peroxisome proliferators-ac- tivated receptor-γcoactivator-1)和解偶联蛋白1基因等的表达,是促进褐色脂肪细胞形成的关键调控因子。BMP7 (Bone morphogenetic proteins 7)也可激活PRDM16和PGC-1α等基因的表达,刺激BAT细胞分化及产热增强。COXII (cyclooxygenase-2)、PPARα (peroxisome proliferator-activated receptor α)、PGC-1α是褐色脂肪细胞分化与代谢中的关键调控因子。在WAT中COXII是肾上腺素信号通路的一个效应分子,对于WAT中诱导形成褐色脂肪细胞是必需的。PPARα在BAT中的表达水平高于WAT,能诱导BAT中的产热相关基因的表达及原代褐色脂肪细胞的生成。PGC-1α也能诱导WAT中褐色脂肪细胞的形成,在褐色脂肪细胞分化的过程中PGC-1α表达量上升。本论文对脂肪转化过程中关键因子进行研究,并且对于小型哺乳动物的脂肪转化研究给出一些展望。
Adipose tissue is divided into brown adipose tissue (BAT) and white adipose tissue (WAT). BAT was a specialized thermogenic tissue, which was the main site of nonshivering thermogenesis in small mammals. PR domain-containing 16 (PRDM16) was a brown adipose determination factor. It was selectively expressed in BAT and induced the expression of α subunit of peroxisome proliferators-activated receptor-γcoactivator-1 (PGC-1α) and uncoupling protein 1. BMP7 could stimulate brown adipocyte differentiation and enhance thermogenesis by activating the expression PRDM16 and PGC-1α genes. COXII, PPARα and PGC-1α were the key regulatory factors of differentiation and metabolism in brown adipocyte. COXII was an effector molecule of adrenergic pathway in WAT. It was necessary for the formation of brown adipocytes. PPARα expression level in BAT was higher than WAT. It could induce the expression of thermogenesis related genes and promote the generation of primary brown adipocyte. PGC-1α also could induce the formation of brown adipocytes in WAT. In the process of brown adipocytes differentiation, PGC-1α expression level increased. In this paper, the key factors of transformation of adipose tissue were studied, and some prospects for the study of the fat tissue transformation of small mammals were given.

褐色脂肪组织分化及其调节机制研究进展
Brown Adipose Tissue Differentiation and Control Mechanism
 [PDF]

张麟, , 蔡金红, 练硝, 王政昆
Bioprocess (BP) , 2011, DOI: 10.12677/bp.2011.12004
Abstract: 人类和其他哺乳动物主要有两种脂肪组织:白色脂肪组织(又分为内脏和皮下两大类)和褐色脂肪组织;相对于内脏白色脂肪组织,皮下白色脂肪组织和褐色脂肪组织有利于改善糖代谢和能量消耗。人体内出现两种褐色脂肪组织的分化途径。BMP7 (bone morphogenetic proteins 7)是转化生长因子-β超家族成员(transforming growth,TGF-β)的多功能分泌型信号分子,在胚胎发生、个体形成、非对称器官的形成、胚胎的发育和损伤组织的修复中起关键作用。最近发现BMP7能促进褐色脂肪细胞的分化,可能是由于BMP7诱导PRDM16及PGC-1α等的基因表达,进一步增加了UCP1等基因的表达,最终诱导褐色脂肪细胞中线粒体的生物生成和随后的细胞分化。这些研究显示通过激活 BMP7和PRDM16 来增加褐色脂肪组织在治疗肥胖及相关疾病方面具有很大潜力。最近采用监测癌症及癌症转移的氟化脱氧葡萄糖–正电子发射计算机断层显像技术与(或)X-射线断层显像技术(18F-FDG-PET/CT)扫描技术并结合定位组织取样进行分子鉴定发现在成年人体内的确存在有功能活性的褐色脂肪组织,人们将其作为一个寻找治疗肥胖症新方法的重要靶标。
Humans and other mammals have two main adipose tissue depots: white adipose tissue (include visceral- and subcutaneous- white adipose tissue), and brown adipose tissue, each of which possesses unique cell-autonomous properties. In contrast to visceral adipose tissue, which can induce detrimental metabolic ef-fects, subcutaneous white adipose tissue and brown adipose tissue have the potential to benefit metabolism by improving glucose homeostasis and increasing energy consumption. BMP7 (bone morphogenetic proteins7) is one of members of the transforming growth factor-β (TGF-β) superfamily and control multiple key steps of embryonic development and differentiation. In recent studies, people found that BMP7-induced UCP1 ex-pression was markedly diminished in brown preadipocytes deficient in both PRDM16 and PGC (PGC-1α and PGC-1β), it ultimately induces BAT mitochondrial cells in the biological production and cell differentiation. There are two ways in development of brown adipose tissue. These researches indicate the potential to treat obesity and related diseases through activating BMP7 and PRDM16 to produce brown adipose tissue. Re-cently, using 18F-fluorodeoxyglucose (18F-FDG) positron-emission tomographic and computed tomographic (PET-CT) scans showed that adults retain metabolically active BAT depots that can be induced in response to cold and sympathetic nervous system activation. These findings high light BAT as a potenial relevant tar get for pharmacological and gene expression manipulation to combat human obesity. We reviewed the recent re-search progresses of BAT in human and its potential functional significance.
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