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Vitamin C is necessary to the growth and metabolism for human beings. Vitamin C is mainly from fruits, vegetables and medicine, so it is one of the important standards to evaluate the quality of the food nutrition and medicine. With the growing concern of food safety, the quantitative analysis of vitamin C in food nutrition and pharmaceuticals has attracted more and more attention. In recent years, determination techniques of vitamin C in food and pharma- ceuticals have been developed rapidly, including titrimetric method, spectrophotometric method, electrochemical me- thod, chemiluminescence method, flow injection analysis, liquid chromatography and indirect atomic adsorption me- thods, etc. In this work, some principles, characteristic and the recent progress of reported methods have been summarized and compared.