oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 29 )

2018 ( 477 )

2017 ( 457 )

2016 ( 520 )

Custom range...

Search Results: 1 - 10 of 38744 matches for " 姜安 "
All listed articles are free for downloading (OA Articles)
Page 1 /38744
Display every page Item
Ca2+信号通路介导白藜芦醇诱导SGC-7901肿瘤细胞凋亡的研究
Effect Mechanism of Resveratrol Induces SGC-7901 Apoptosis via Ca2+ Signal Pathway
 [PDF]

, 子涛
World Journal of Cancer Research (WJCR) , 2011, DOI: 10.12677/WJCR.2011.11001
Abstract:

目的:研究白藜芦醇(Res)诱导SGC-7901肿瘤细胞凋亡的Ca2+信号通路的机制。方法:MTT法测定Res对SGC-7901细胞抑制率;流式细胞仪检测Res对肿瘤细胞凋亡和肿瘤细胞周期的影响;荧光显微镜从形态学鉴定肿瘤细胞凋亡。激光共聚焦法测定白藜芦醇对肿瘤细胞Ca2+活性的影响。结果:Res明显抑制肿瘤细胞的增值,且呈一定剂量依赖关系,其IC50为164.69 μmol?L–1,当Res剂量为44、88、176 μmol?L–1时,肿瘤细胞的抑制率为:29.693%,33.986%,85.634%;此外,肿瘤细胞周期G1期细胞的比例减少,S期细胞的比例增加,G2/M期减小;通过形态学观察发现,随着Res剂量的增加,镜下凋亡细胞比例逐渐增加,肿瘤细胞的核浆比减小,细胞核高度固缩,染色程度加深,凋亡小体的数量不断增加。其对Ca2+含量的影响分别为18.0463 ± 1.0798、19.7550 ± 2.1129和26.6300 ± 2.0813。结论:Res明显抑制SGC-7901细胞的增殖且诱导其凋亡并影响Ca2+信号通路。
Objective: To study effect of resveratrol (Res) induce SGC-7901 cell apoptosis via Ca2+ signal. Methods: Use the MTT method to test the inhibition rates of Res in SGC-7901 tumor cells; Use FCM to detect the influence of the Res on tumor cell apoptosis and cell cycle; Use fluorescence microscope to identify the tumor cell apoptosis in the morphology. Results: Res inhibit SGC-7901 cell proliferatrion in a dose- dependent manner, Its IC50 is 164.69 μmol?L–1. When the concentration of Res is 44, 88, 176 μmol?L–1, the rates of proliferatrion are 29.693%, 33.986%, 85.634%. G1 term is gradually diminished, S term become gradually increasement. G2/M term become gradually reduction. According to the morphology, the apoptosis body become increase, tumor cell’s caryoplasm become reduction and tumor cell karyopyknosis, the extent of the colour become deeply with the concentration of the Res. content of Ca2+ are 18.046 ± 1.0798; 19.7550 ± 2.1129; 26.6300 ± 2.0813 respectively. Conclusion: Res can inhibit the tumor cell prolieratrion and induce the tumor cell apoptosis via Ca2+ sigal pathway

行政的现代化与行政程序制度

中外法学 , 1998,
Abstract:
行政法的基本原则

中外法学 , 1989,
Abstract:
论国家侵权责任的构成

中外法学 , 1991,
Abstract:
行政法制在中国的生长

中外法学 , 1989,
Abstract:
我国公务员制度的建立和主要内容

中外法学 , 1994,
Abstract:
授权、分权、控权——平衡行政法生存的“理”

中外法学 , 1996,
Abstract:
澳大利亚“新行政法”的产生及其主要内容

中外法学 , 1995,
Abstract:
行政强制法立法若干争议问题之我见

法学家 , 2010,
Abstract: 本文对《行政强制法(草案)》(三审稿)中存在的三项主要争议从实践层面进行了系统探讨。作者认为,行政强制设定权应配置给法律、行政法规和地方性法规,但在条文表述上应修改得更加严谨;行政强制实施体制原则上应实现裁执分离,行政机关作出行政强制决定后,应申请人民法院审查并裁定,但一律由行政机关负责执行;行政强制基本原则应包括四项行政强制法定原则、比例原则、教育与强制相结合原则和正当法律程序原则。
法律规范行政强制行为的意义和途径

法学家 , 2006,
Abstract:
Page 1 /38744
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.