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Search Results: 1 - 10 of 61272 matches for " 傅博强 "
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水体沉积物中有机碳和有机分子碳稳定同位素研究进展
,严重玲,
海洋科学 , 2007,
Abstract:
乙肝病毒生物标志物及其检测方法研究进展
Progress of Hepatitis B Virus Biomarkers and Detection Methods
 [PDF]

张力玲, 隋志伟, 刘瑛颖, 王晶, , 陈文, 王佳媛
Medical Diagnosis (MD) , 2013, DOI: 10.12677/MD.2013.34013
Abstract:
全世界约有3.5亿人感染乙型肝炎病毒(Hepatitis B virus, HBV),其中中国有约1.2亿人。应用HBV生物标志物检测,对于HBV的诊断、防治至关重要。目前我国针对HBV的检测方法非常多,这些检测方法基于不同的HBV生物标志物和不同的检测原理,其在临床上的应用以及检测效果也有所不同,因此了解HBV生物标志物及其检测方法的最新进展就显得更为重要。本文逐一介绍了HBV的理化特征、结构、生物标志物以及检测方法的研究进展,以供HBV的临床检测参考。
Hepatitis B is one of the most common infectious diseases around the world. About 350 million people are infected with hepatitis B virus totally in the world, and 120 million people are infected in China. It is very important to apply biomarker detection for diagnosis, prevention and treatment of HBV. At present, there are many detection methods of HBV based on different biomarkers and principles, and they have different clinic application and detection effects, so the progress of the HBV biomarkers and detection methods is more and more important. This article introduced the physical and chemical characteristics, structure, biomarkers of HBV and the research progress of analytical methods, in order to provide the reference for the clinical detection of HBV.
中国乙肝病毒核酸检测试剂盒现状
Present Status of Hepatitis B Virus Nucleic Acid Detection Kit in China
 [PDF]

隋志伟, 冉令磊, 张玲, 陈文, 王晶, , 王佳媛
Medical Diagnosis (MD) , 2014, DOI: 10.12677/MD.2014.42004
Abstract: 我国是乙型肝炎病毒(Hepatitis B virus, HBV)的高发区,应用核酸扩增技术对HBV核酸生物标志物进行定量检测是HBV诊断和治疗监测中的重要方法。然而,目前我国市场上的商业化HBV核酸检测的试剂盒很多,有必要对不同厂商生产的乙肝病毒核酸检测试剂盒进行评价和分析。本文对乙肝病毒核酸检测方法、乙肝病毒核酸检测试剂盒现状和比较分析等方面作一介绍,以供试剂盒使用者和研发者参考。
China is a high incidence of hepatitis B virus; nucleic acid amplification (PCR) quantitative detection of HBV nucleic acid biomarkers is one of the important means in diagnosis and treatment monitoring of hepatitis B virus. However, there are many commercial HBV nucleic acid detection kits in the Chinese market, it is necessary to evaluate and analyze the kit for hepatitis B virus nucleic acid production of different manufacturers. This article introduced HBV nucleic acid detection methods, present status and comparative analysis of HBV nucleic acid detection kits to provide the reference for the users and developers.
荧光标记寡核苷酸的反相离子对色谱分析
黎朋,王晶,高运华,武利庆,盛灵慧,
分析化学 , 2009,
Abstract: 建立荧光标记寡核苷酸反相离子对色谱分析方法,优化了流动相醋酸三乙胺浓度(0~0.15mol/L),pH4.5~7.0和洗脱强度等色谱条件。对5-mer,10-mer和15-mer非标记和5′-羧基荧光素(5′FAM)标记寡核苷酸的保留进行比较分析,研究荧光标记寡核苷酸的保留机理,并分离TaqManTM探针等多种常用荧光标记寡核苷酸。结果显示,不同长度荧光标记寡核苷酸在0.01mol/L醋酸三乙胺,pH7.0的条件下获得最大分离。荧光标记寡核苷酸的保留与非标记寡核苷酸有明显差异,两者可完全分离。在一定长度范围内非标记寡核苷酸随长度的增加,保留时间增长;相反,荧光标记寡核苷酸的长度增加,保留时间减短。荧光染料疏水性对其标记的寡核苷酸在反相柱中的保留有较大影响,荧光染料疏水性越强,其标记寡核苷酸保留时间越长。但疏水性的影响程度随标记寡核苷酸长度增加而逐渐变小。
POE-g-PMAH反应性增容PA1010/PP共混物的性能研究
杨风霞,杜荣昵,,陈龙,杨静晖,,张琴
高分子学报 , 2010, DOI: 10.3724/SP.J.1105.2010.00366
Abstract: 乙烯-辛烯共聚物-g-聚马来酸酐(POE-g-PMAH)作为反应性增容剂,采用熔体共混的方法制备了PA1010/PP共混物,通过扫描电镜(SEM)、力学性能、傅立叶变换红外光谱(FTIR)和示差扫描量热(DSC)测试,研究了POE-g-PMAH对PA1010/PP共混物的增容作用.结果表明,POE-g-PMAH的加入可以减小共混物的相区尺寸,当PA1010/PP/POE-g-PMAH=70/30/15时,分散相尺寸小而均匀;FTIR结果表明接枝在POE上的马来酸酐基团和PA1010在熔融共混期间发生了化学反应;DSC研究结果表明共混体系中PA1010和PP的结晶温度和结晶度随POE-g-PMAH的加入而降低,表明POE-g-PMAH的增容作用对PA1010和PP的结晶有抑制作用.力学性能测试结果表明随着POE-gPMAH的增加,共混物的冲击强度逐渐增加,当POE-g-PMAH含量增加到15%时,干态冲击强度达到21.13kJ/m2,是不加增容剂的3.1倍,而拉伸和弯曲强度可以保持较高水平.POE-g-PMAH的增容机理在于其支链中的马来酸酐能与PA1010中的胺基(NH2—)发生化学反应,而主链POE与PP有较好的亲和性,从而降低界面张力,减少相区尺寸,大幅度提高力学性能.
茶叶粗多糖的提取及纯化研究
周鹏,谢明勇,,王远兴
食品科学 , 2001,
Abstract: ?利用sevag法脱游离蛋白、乙醇沉淀、二次透析等手段得到了初步纯化的茶叶粗多糖,该粗多糖经sephadexg100凝胶柱层析纯化,0.1mol/lnacl洗脱,获得一较大组分,命名为tgp,通过凝胶渗透法测定其平均分子量为6×104道尔顿。
茶叶中多糖含量的测定
,谢明勇,聂少平,周鹏,王远兴
食品科学 , 2001,
Abstract: ?茶样经80%乙醇回流除去单糖、双糖、低聚糖、茶多酚、氨基酸等杂质后用水提取,蒽酮-硫酸法比色测定。用精制茶多糖测得茶多糖对葡萄糖的换算因子。对江西婺源不同茶场茶叶中多糖的含量进行了测定,并与其它产地、品种的茶叶多糖含量进行了比较。研究结果表明,此测定方法简便,供试液在4h内显色稳定,重现性较好,平均回收率为99.2%±1.80%,rsd=1.82%(n=3)。江西婺源绿茶中多糖含量在4.17%~6.35%之间,ctc红碎茶多糖含量为3.10%~3.53%,其含量与茶叶品种、等级、生长海拔高度、产地有关。
金黄色葡萄球菌标准物质的研制
薛蕾,隋志伟,张玲,王晶,,刘瑛颖,郝林
食品科学 , 2015,
Abstract: ?将金黄色葡萄球菌添加到复合保护剂中,经冻干制备成干粉状标准物质,通过比较冻干前后活菌数来验证保护剂保护效果,并研究标准物质的均匀性和稳定性。结果表明复合保护剂的保护率达到51.74%,可以在冻干过程中有效保护菌体,制备的金黄色葡萄球菌标准物质均匀性良好,可以稳定保存半年。该标准物质可用于食品检测实验室质量控制和食品中金黄色葡萄球菌检测结果的评价。
连续油管下入作业力学特性研究
Study on Mechanical Properties of Coiled Tubing Setting Operation
 [PDF]

白田增, 任勇, 杨春华, 吴德,, 小勇
Journal of Oil and Gas Technology (JOGT) , 2019, DOI: 10.12677/JOGT.2019.415068
Abstract:
连续油管在作业过程中,井下液体内外压及油管失稳弯曲等因素对连续油管造成极大影响。基于钻机作业下的井深坐标参数,建立井眼轨迹几何模型,采用复化辛普森求积公式的数值积分方法计算出井眼轴坐标参数;求解出的坐标参数,分别对连续油管的环空压力、轴向力及弯矩等连续油管力学特性进行分析计算。该研究为连续油管提供了力学分析基础。
During the operation of coiled tubings, great influence would be induced because of the internal and external pressure of downhole fluid, tubing instability and bending as well as other factors. Based on the well depth coordinate parameters of drilling rig, the geometric model of well trajectory was established, and the numerical integration method of Simpson quadrature formula was used to calculate the well shaft coordinate parameters. Based on the obtained coordinate parameters, the mechanical properties of coiled tubing, such as annulus pressure, axial force and bending moment, were analyzed and calculated respectively. Based on the actual borehole trajectory parameters, the mechanical properties were analyzed with each equation. It provides a basis for analyzing the mechanical properties of coiled tubings.
巨大芽孢杆菌D01吸附金(Au3+)的谱学表征
林种玉,吴剑鸣,,薛茹,周剑章,郑泉兴,刘月英,锦坤
化学学报 , 2004,
Abstract: 对休眠的巨大芽孢杆菌(Bacillusmegatheriun)D01菌体吸附Au3+的作用过程进行了谱学表征.运用AAS考察了pH、时间和温度对D01菌体吸附Au3+过程的化学动力学和热力学相关参数的影响.D01菌粉中硫元素含量的EDX分析说明该菌体中对Au3+具有还原作用的L-半胱氨酸和蛋氨酸的含量极少;D01菌体水解后葡萄糖含量的UV-vis测定说明该菌体水解产物中含有一定量的还原糖.空白的和吸附Au3+的D01菌体的FTIR检测表明该菌体细胞壁肽聚糖层糖类化合物的羟基和肽链侧链氨基酸残基离子化羧基为吸附Au3+的活性基团;肽聚糖层部分多糖的水解产物低聚糖、二糖及单糖等还原糖的半缩醛羟基游离态醛基为电子供体,将Au3+原位还原成Au0.葡萄糖和Au3+相互作用的XRD和FTIR表征证明Au3+是在还原糖的醛基上直接被还原成Au0.
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