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Search Results: 1 - 10 of 179813 matches for " édila Vilela de Rezende Von; "
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Análise fisiológica e ultra-estrutural durante o desenvolvimento e a secagem de sementes de soja
Silva, Paulo de Albuquerque;Diniz, Kênia Almeida;Oliveira, Jo?o Almir;Pinho, édila Vilela de Rezende Von;
Revista Brasileira de Sementes , 2007, DOI: 10.1590/S0101-31222007000200003
Abstract: during development and drying, a number of protection mechanisms act on the maintenance of seed physiological quality. some of these mechanisms maintain the integrity of the cells, which is fundamental to the perfect functioning of cell metabolism. the study of the ultra-structure enables visualization of damage caused by drying in the absence of protection mechanisms. in this research, damage occurred during the soybean seed development and drying was studied by means of scanning electron microscopy (sem). in addition, seeds were also evaluated regarding the physiological quality. thus, seeds in different developmental stages were collected and submitted to different drying temperatures and treatments. evaluations were made concerning the seed physiological quality by means of the germination test, cold test, emergence test under controlled conditions and electrical conductivity test. the damages were visualized by means of scanning electron microscopy utilizing the leo evo40 microscope. the results showed that the soybean seeds become desiccation tolerant when they possess around 30% water content. drying of seeds with moisture content above 30% caused reduction in cellular volume, disorganization of the cell walls and decrease in seed physiological quality. higher germination values were observed in seeds with 30% moisture content in the field, whereas higher values of vigor were observed in seeds with 40% of moisture content in the field.
Osmotic priming methodologies in relation to the physiological performance of rangpur lime seeds (Citrus limonia Osbeck)
Dantas, ísis Barreto;Guimar?es, Renato Mendes;Pinho, édila Vilela de Rezende Von;Carvalho, Maria Laene Moreira de;
Revista Brasileira de Sementes , 2010, DOI: 10.1590/S0101-31222010000300016
Abstract: several mechanisms have been used to promote rapid germination of citrus seeds and uniform seedling emergence. we evaluated the effects of osmotic priming on the physiological performance of rangpur lime seeds (citrus limonia osbeck). seeds were treated with 30 g of captan and 10 g of tecto 600 in 20-litre batches and stored, without drying, at 10 oc and 50% relative humidity for periods of 3, 6 and 9 months. after each period, seeds were primed at 25 oc, in the light, by immersion in poliethylenoglicol (peg 6000), potassium nitrate (kno3) and 70% peg 6000 plus 30% kno3, all at an osmotic potential of -1.1mpa, for priming periods of 3, 6, 9 and 12 days. percentage germination, tray emergence and the emergence rate index (eri) were evaluated. priming in peg 6000 solution, independent of priming period, or in kno3 or peg 6000 plus kno3 for up to 9 days, were efficient at improving the physiological performance of seeds stored for up to 3 months. osmotic priming appears to be a promising technique for improving the physiological quality of rangpur lemon seeds.
Consequências da remo??o do limbo foliar em diferentes estádios reprodutivos da cultura do milho em duas épocas de semeadura
Lima, Tiago Geraldo de;Pinho, Renzo Garcia Von;Pereira, José Luiz de Andrade Rezende;Brito, André Humberto De;Von Pinho, édila Vilela de Resende;
Bragantia , 2010, DOI: 10.1590/S0006-87052010000300007
Abstract: there is little information, in tropical areas concerning the effects of blade removal on maize crop characteristics. the aim of this research was to evaluate the effects of defoliation during the grain filling period. the experiment was arranged as a factorial complete randomized block design with blade removal at two different plant insertion points (above and below corn ear), with two percentages of blade removal (50% and 100%), and four phenological stages (r1, r2, r3, and r4). there were three blocks. two identical experiments were installed, in 12/11/2005 and 21/12/2005. the hybrids gnz 2004 and p 30f33 were evaluated in both experiments. blade removal treatment above corn ear promoted higher grain yield loss in all reproductive stages in relation to the blade removal below corn ear. blade removal treatment did not change with phenological stages. results of blade removal below corn ear were similar to 50% of defoliation treatment above the ear. in addition, corn plant lodging was little affected by manual blade removal.
Identifica??o de cultivares de milho, feij?o, algod?o e soja por meio de enzimas e proteínas resistentes ao calor
Menezes, Mariney de;Pinho, édila Vilela de Resende Von;Pereira, Ant?nio Marcos de Andrade Rezende;Oliveira, Jo?o Almir de;
Revista Brasileira de Sementes , 2008, DOI: 10.1590/S0101-31222008000200014
Abstract: in this study the polymorphism and stability of isoenzymes and heat-resistant proteins in corn, bean, cotton, and soybean seeds with different levels of physiological quality were evaluated. the alcohol dehydrogenase, catalase, esterase and superoxide dismutase enzymes and simultaneous analysis were effective in identifying eight corn cultivars. it was observed for the bean cultivars that the peroxidase enzyme allowed differentiation of the carioca bean cultivar from the others but, the peroxidase enzyme pattern varied in seeds with low-germination percentage. the varieties of cotton could not be differentiated by esterase enzyme, superoxide dismutase, diaphorase and malate dehydrogenase. the conquista soybean cultivar was separated by superoxide dismutase and esterase enzyme systems and brs-154 was separated by esterase. heat-resistant protein patterns showed polymorphism and were stable for corn cultivar identification.
Qualidade fisiológica de sementes de milho na presen?a de bioestimulantes
Silva, Tanismare Tatiana de Almeida;Von Pinho, édila Vilela de Rezende;Cardoso, Deisy Lúcia;Ferreira, Clarissa Alves;Alvim, Patrícia de Oliveira;Costa, Aldo Augusto Fernades da;
Ciência e Agrotecnologia , 2008, DOI: 10.1590/S1413-70542008000300021
Abstract: growth regulators can influence the physiological quality of seeds as well as the activity of enzymes involved in germination. they may, further, present in their formulation micronutrients which act mainly as enzyme catalysts. in this research work, hybrid corn seeds of the cultivar gnz 2004 and line le 57 were treated with the following biostimulants and dosages: 1.stimulate? 12.5ml/kg of seed, 2.cellerate? 10,0ml/kg, 3.mixtue of cellerate?+ stimulate? (10.0ml/kg + 12.5 ml/kg), 4.booster? 100 ml/20kg and 5.control. soon after the treatment, the seeds were submitted to the tests of germination, accelerated ageing and cold and seedling emergence tests. in addition, mass of dry matter of seedlings, shoot and roots and the activities of the enzymes á-amylase, catalase, esterase and peroxidase were evaluated. in the biostimulanttreated seeds, there were no improvements in the quality of seeds and under stressful conditions, the use of the biostimulants stimulate?+cellerate? and cellerate? reduced the physiological quality of corn seeds. increased activity of the peroxidase enzyme was found in seeds treated with stimulate?, cellerate? and stimulate?+cellerate? . increased activity of the enzyme esterase was observed in seeds treated with stimulate?+ cellerate? pointing out phytotoxicity of them.
Qualidade fisiológica de sementes de milho, feij?o, soja e alface na presen?a de extrato de tiririca
Muniz, Fabiana Rezende;Cardoso, Maria das Gra?as;Von Pinho, édila Vilela Resende;Vilela, Michelle;
Revista Brasileira de Sementes , 2007, DOI: 10.1590/S0101-31222007000200026
Abstract: cyperus rotundus (sedge) is a perennial plant that is difficult to control. its subterranean organs produce inhibitors that can interfere with the germination and growth of sprouts and plants of various species, a phenomenon called allelopathy. the inhibition of seed germination may be associated with the interference of allelopathic substances in the activity of key enzymes in the germination process. the physiological quality and the activity of the enzymes involved in the germination process of corn, bean, soybean, and lettuce seeds submitted to sedge bulb extract were evaluated. the seeds were germinated in a substrate containing 10 g l-1 and 100 g l-1 concentrations of sedge bulb extract and distilled water. the physiological quality was evaluated by means of germination and vigor tests. the activities of superoxide dismutase and esterase were evaluated for all the species, catalase for corn and bean seeds, peroxidase and endo-b-mananase for lettuce seeds, glutamate-oxaloacetate transaminase and a-amylase for corn seed and glucose-6-phosphate dehydrogenase for soybeans. a decrease in germination of lettuce seeds with an increase in the concentration of the extract was observed. the inhibition of corn and bean seed germination and the stimulus to germination of soybeans submitted to an extract concentration of 10 g l-1, as well as the inhibition of soybean germination in the presence of a concentration of 100 g l-1 of extract were observed. a reduction in the activities of superoxide dismutase, endo-b-mananase, peroxidase and a-amylase with an increase in the concentration of extract was detected. an increase in the activities of glutamate-oxaloacetate transaminase and glucose-6-phosphate dehydrogenase with an increase in extract concentration was observed, indicating a loss in seed quality. a lower esterase activity was found in lettuce seeds submitted to substrate containing an extract concentration of 100 g l-1, while a lower activity of this enzyme was obser
Physiological quality of coffee seeds produced under different levels of solar radiation and maturation stages Qualidade fisiológica de sementes de café produzidas sob diferentes níveis de radia o solar e estádios de matura o
Danielle Pereira Baliza,Franciele Caixeta,édila Vilela de Resende Von Pinho,Rodrigo Luz da Cunha
Revista Brasileira de Sementes , 2012, DOI: 10.1590/s0101-31222012000300008
Abstract: In Brazil, although the coffee plantations are predominantly grown under full sunlight, the use of agroforestry systems can lead to socioeconomic advantages, thus providing a favorable environment to the crop by promoting its sustainability as well as environmental preservation. However, there is a lack of information on physiological quality of the coffee seeds produced under different levels of solar radiation. Within this context, the objective of this study was to evaluate the influence of different levels of solar radiation and maturation stages on the physiological quality of coffee (Coffea arabica L.) seeds, cv. Acaiá Cerrado MG-1474. Three levels of solar radiation (plants grown under full sunlight; under plastic screens of 35% shading; and under plastic screens of 50% shading) and three maturation stages (cherry, greenish-yellow and green) were assessed. Physiological quality of seeds was assessed by using germination test, first count of germination, abnormal seedlings, dead seeds, and seedlings with open cotyledonary leaves. Electrophoretic analysis of isoenzymes catalase, esterase, superoxide dismutase and peroxidase was also performed. With the evolution of development the coffee seeds presents increases on physiological quality, and at its beginning the seeds show improvements on quality with the reduction of solar radiation. No Brasil, embora haja predomínio do cultivo do cafeeiro a pleno sol, o uso de sistemas agroflorestais pode induzir vantagens sócioecon micas apresentando um ambiente favorável para a cultura, promovendo sustentabilidade e preserva o do ambiente. No entanto, faltam informa es sobre a qualidade fisiológica das sementes de café produzidas sob diferentes níveis de radia o solar. Neste contexto, o objetivo deste trabalho foi avaliar a influência de diferentes níveis de radia o solar e estádios de matura o, na qualidade fisiológica de sementes de café (Coffea arabica L.), cultivar Acaiá Cerrado MG-1474. Foram utilizados três níveis de radia o solar (plantas a pleno sol e sob sombrites de 35% e de 50% de sombra) e três estádios de matura o (cereja, verde-cana e verde). A qualidade fisiológica das sementes foi avaliada por meio do teste de germina o, primeira contagem, plantulas anormais, sementes mortas e plantulas com folhas cotiledonares abertas. Foi realizada ainda a análise eletroforética das isoenzimas catalase, esterase, superóxido dismutase e peroxidase. As sementes de café apresentam aumento da qualidade fisiológica com a evolu o do seu desenvolvimento e, no início do seu desenvolvimento, apresentam melhori
Physiological quality and amylase enzyme expression in maize seeds Qualidade fisiológica e express o das enzimas amilases em sementes de milho
Gustavo Evangelista Oliveira,Renzo Garcia Von Pinho,Thaís de Andrade,édila Vilela de Resende Von Pinho
Ciência e Agrotecnologia , 2013,
Abstract: The physiological quality of maize seeds is affected by the genotype. Thus, the study of expression of genes associated with this characteristic is important in the genotype selection process in breeding programs. The aim of this research was to study the expression of amylase enzymes associated with physiological quality of maize seeds with different genotypes and seed sizes. We further sought to assess the expression of these enzymes in dry and soaked seeds The experiment was conducted in the experimental area and the Central Seed Laboratory of the Department of Agriculture of the Universidade Federal de Lavras. Seeds of four maize inbred lines were used, classified in two sizes. The physiological quality of the seeds was evaluated by means of germination, seedling emergence, seedling emergence speed index and accelerated aging test. Expression of the alpha amylase enzyme was evaluated by the electrophoresis technique and expression of the alpha amylase B73, alpha amylase (LOC542522) and beta amylase 5 (amyb5) genes was studied by the qRT-PCR technique in dry and soaked seeds of the inbred lines. There is differentiated expression of amylase enzymes in maize seeds of inbred lines with different levels of physiological quality. higher expression of amylase enzymes is observed in soaked maize seeds. The expression of transcripts is higher in smaller as wellas in soaked maize seeds of inbred lines. A qualidade fisiológica de sementes de milho é influenciada pelo genótipo. Assim, o estudo da express o de genes associados a essa característica é importante no processo de sele o de genótipos em programas de melhoramento. O objetivo neste trabalho foi estudar a express o das enzimas amilases associadas à qualidade fisiológica de sementes de milho, de diferentes genótipos e tamanhos de sementes. Objetivou-se ainda avaliar a express o dessas enzimas em sementes secas e embebidas. O experimento foi conduzido em área experimental e no Laboratório Central de Sementes do Departamento de Agricultura da Universidade Federal de Lavras. Foram utilizadas sementes de quatro linhagens de milho, classificadas em dois tamanhos. A qualidade fisiológica das sementes foi avaliada por meio dos testes de germina o, emergência de plantulas, índice de velocidade de emergência de plantulas e envelhecimento artificial. A express o da enzima -amilase foi avaliada pela técnica de eletroforese e a express o dos genes alpha amylase B73, alpha amylase (LOC542522) e beta amylase 5 (amyb5), foi estudada pela técnica de qRT-PCR em sementes secas e embebidas das linhagens. Há express o di
Tolerancia de sementes de linhagens de milho à alta temperatura de secagem
José, Solange Carvalho Barrios Roveri;Pinho, édila Vilela de Resende Von;Pinho, Renzo Garcia Von;Silveira, César Martoreli da;
Ciência e Agrotecnologia , 2004, DOI: 10.1590/S1413-70542004000500019
Abstract: high drying temperature tolerant cultivars provide a reduction in the drying period, a critical phase of the corn seeds (zea mays l.) production system. in this research the tolerance of corn lines seeds to high drying temperature was evaluated by the germination and vigor tests. seeds were handpicked in ears with water content around 35% and dried artificially at 45oc up to 11% water content. then, the seeds were submitted to the first and final germination counting tests, accelerated aging, cold test without soil and electrical conductivity. there were significant differences in the germination and vigor values of seeds from different lines, being classified into tolerant and intolerant. the results permitted to conclude that sensitivity of seeds to high drying temperature injury depends on the lines.
Padr?o eletroforético de proteínas resistentes ao calor em sementes de milho
José, Solange Carvalho Barrios Roveri;Von Pinho, édila Vilela de Resende;Von Pinho, Renzo Garcia;Silveira, César Martoreli da;
Pesquisa Agropecuária Brasileira , 2005, DOI: 10.1590/S0100-204X2005000200003
Abstract: several mechanisms are involved in the acquisition and maintenance of desiccation tolerance by the seeds. one of these mechanisms is related to the induction of heat resitant proteins. the objective of this work was to evaluate changes in the electophorectic patterns of the heat resistant proteins of corn seeds submitted to high drying temperature associating their expression with drying tolerance. seeds of breeding lines, simple hybrids and respective reciprocals hybrids were utilized. the seeds were harvested with approximately 35% of water content and dried at 45°c. seeds of lines dried under shadow were used as control and the physiological quality was evaluated by germination test. the heat resistant proteins were extracted from embryonic axis of seeds in tris-hcl 0.05 m buffer. it was not possible to determine a specific band of heat resistant proteins which can be of use as a molecular marker of tolerance to high drying temperature. there was stability in the patterns of the bands of proteins of seeds submitted to artificial drying compared to the ones after shadow drying even for the lines of high variation in the germination values. the electrophorectic patterns of the heat resistant proteins were similar for both the hybrid seeds and respective reciprocals.
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