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Esporos e toxinas de Clostridium botulinum dos tipos C e D em cacimbas no Vale do Araguaia, Goiás  [cached]
Souza Aires M.,Marques Dercino F.,D?bereiner Jürgen,Dutra Iveraldo S.
Pesquisa Veterinária Brasileira , 2006,
Abstract: Foram avaliadas a ocorrência e distribui o de esporos e toxinas de Clostridium botulinum tipos C e D em 300 cacimbas empregadas como bebedouro de bovinos em 130 propriedades rurais localizadas em 12 municípios do Vale do Araguaia, Estado de Goiás. A presen a de esporos foi determinada indiretamente pelo cultivo em meio de cultura, seguido da inocula o e neutraliza o em camundongo das amostras de sedimento do interior das cacimbas, e do solo superficial e fezes de bovinos, coletadas ao seu redor. A presen a de toxina foi avaliada diretamente pela inocula o em camundongo do sedimento filtrado das cacimbas, também seguida da neutraliza o em camundongo com antitoxinas C e D. A presen a de esporos de C. botulinum foi significativamente maior (p<0,05) nas fezes de bovinos (31%), quando comparadas com os resultados das amostras de solo superficial (19%) e dos sedimentos (10%). Foram detectadas toxinas botulínicas dos tipos C, D, ou classificadas como pertencentes ao complexo CD, em seis amostras (2%) das 300 cacimbas. Das 130 propriedades trabalhadas, em 122(93,85%) foram encontrados esporos ou toxinas de Clostridium botulinum em pelo menos uma das variáveis pesquisadas, enquanto somente 8(6,15%) n o apresentaram qualquer contamina o A idade e profundidade das cacimbas estiveram associadas com a freqüência de detec o de esporos e toxinas. Assim, quanto mais velhas e rasas, maior a freqüência do isolamento de esporos e toxinas. A contamina o das cacimbas do Vale do Araguaia goiano com esporos e toxinas do Clostridium botulinum tipos C e D demonstra o risco potencial permanente e crescente para a ocorrência da intoxica o botulínica de origem hídrica nos bovinos.
Avalia??o farmacognóstica de geoprópolis de Melipona fasciculata Smith da Baixada maranhense, Brasil
Dutra, Richard Pereira;Nogueira, Alexandre Michel Costa;Marques, Rodrigo René de Oliveira;Costa, Maria Célia Pires;Ribeiro, Maria Nilce Sousa;
Revista Brasileira de Farmacognosia , 2008, DOI: 10.1590/S0102-695X2008000400010
Abstract: melipona fasciculata smith is a native, social, stingless bee species that produces wax, honey and geopropolis. this work aimed to do a pharmacological evaluation of melipona fasciculata geopropolis, collected in beehives in the municipal districts of arari, s?o bento and s?o jo?o batista, in baixada maranhense, in the state of maranh?o, brazil, seeking to obtain basis for the quality control and standardization of the product. the methodology used included the accomplishment of sensorial analyses, chemical approach, chromatographic profiles and determination of the flavonoids contents. the results demonstrate that the geopropolis have organoleptic characteristics similar to the ones found for the propolis of apis mellifera, presence of phenolic compounds in larger concentration, besides substances of the class of the terpenes and saponins and absence of alkaloids. the chromatographic profiles indicated that the composition and concentration of the chemical substances are different among the samples. the flavonoids contents ranged between 0.17 - 2.6%. the content for most of the samples presented values above the minimum demanded by the brazilian legislation for propolis of apis mellifera. the found data suggest that the qualitative and quantitative variations of flavonoids and other chemical substances in geopropolis are strongly affected for the flora visited by the bees, geographical area and environmental factors.
Concentra??es de am?nio na água da chuva e estimativa de emiss?o de am?nia de rebanhos domésticos de Pinheiro e Viana, Baixada Maranhense
Lima, Richardson G.;Cavalcante, Paulo R. S.;Melo, Odilon T.;Mello, William Z. de;
Química Nova , 2009, DOI: 10.1590/S0100-40422009000900006
Abstract: rainwater samples (bulk deposition samples) were collected in pinheiro and viana. rainwater phs were higher than the range usually expected for unpolluted rain (5.0-5.6). the highest values were found in the beginning of the rainy season. high concentrations of ammonium found in the rainwater samples could probably explain the high phs. grazing animals and other human activities, combined with soil characteristics and climatic conditions can be the potential factors controlling the rainwater concentrations of ammonium in baixada maranhense.
Esporos de Clostridium botulinum em mel comercializado no Estado de S?o Paulo e em outros Estados brasileiros
Ragazani, Adriana Valim Ferreira;Schoken-Iturrino, Ruben Pablo;Garcia, Gisela Rojas;Delfino, Tammy Priscilla Chioda;Poiatti, Maria Luiza;Berchielli, Silvina Pelicano;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000200016
Abstract: infant botulism has been affecting children under one year of age in several regions of the world and honey has been identified as one of the most important source of this food borne disease. despite this, there are scarce data about botulism among children in brazil, specially the type commercial food most implicated in this pathology. this study aimed at investigating the presence of spores of clostridium botulinum in honey commercialized in brazil. one hundred of honey samples commercialized in six different brazilin states (sp, mg, go, ce, mt, sc) were searched for the presence of spores of clostridium botulinum, using thermal shock followed by the inoculation in cooked meat medium (difco?) and incubation in anaerobic conditions. the positives cultures were analyzed by gram stain and seeded in reinforced clostrideo agar (difco?) and sulfito polimixina sulfadiazina -sps (difco?) plates, which were incubated in anaerobic conditions in order to pick up the colonies of this bacteria. the positive colonies were submitted to toxicity test by inoculation in susceptible mice and to biochemical characterization. clostridium botulinum colonies producing actively toxins were detected in 7% of the commercial honey samples, highlighting the relevance of this microorganism for public health due to the high potential risk of honey commercialized in these brazilian regions to cause infant botulism, specially in children under one-year old.
Esporos e toxinas de Clostridium botulinum dos tipos C e D em cacimbas no Vale do Araguaia, Goiás
Souza, Aires M.;Marques, Dercino F.;D?bereiner, Jürgen;Dutra, Iveraldo S.;
Pesquisa Veterinária Brasileira , 2006, DOI: 10.1590/S0100-736X2006000300001
Abstract: the occurrence and distribution of clostridium botulinum spores and toxins type c and d in 300 ponds, used by cattle for drinking on 130 farms located in 12 municipalities of the rio araguaia valley, state of goiás, brazil, was evaluated. the presence of spores was determined indirectly by cultivation in culture medium, followed by inoculation and neutralization in mice of samples of the sediment from the bottom of the raining ponds, from superficial soil and from cattle feces collected to its circuit. the toxin presence was evaluated directly by inoculation in mice of the filtered sediment of the ponds, followed by the neutralization in mice with antitoxins c and d. the presence of c. botulinum spores was significantly more frequent (p<0,05) in the cattle feces (31%), when compared with the results of the superficial soil samples (19%) and the sediments (10%). botulinum toxins of type c and d or classified as belonging to the cd compound were detected in 6 samples (2%) of the 300 ponds. of the 130 worked farms, in 122 (93,85%) ponds clostridium botulinum spores or toxins were found in at least one of the researched variables, whilst ponds on only 8 (6,15%) farms did not present any contamination. age and depth of the ponds were associated with the frequency of detection of botulinum spores and toxins. the older and shallower the ponds were, the larger was the frequency of isolation of the spores and toxins. the contamination of the ponds in the araguaia valley with clostridium botulinum spores and toxins type c and d demonstrates the permanent and growing potential risk for the occurrence of botulism in cattle through drinking water.
SCHISTOSOMIASIS MANSONI IN THE "BAIXADA OCIDENTAL MARANHENSE", STATE OF MARANH?O, BRAZIL: CROSS-SECTIONAL STUDIES PERFORMED IN 1987 AND 1993
CUTRIM, Raimundo Nonato Martins;CHIEFFI, Pedro Paulo;MORAES, José Cássio de;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1998, DOI: 10.1590/S0036-46651998000300006
Abstract: a cross-sectional study on the prevalence of schistosomiasis mansoni in three sites of the "baixada ocidental maranhense" was carried out in 1993 in: alegre (in the municipality of s?o bento), alian?a (in cururupu) and coroatá ii (in the municipality of s?o jo?o batista). results were compared to those of another study performed at the same sites and in similar conditions, in 1987. the entire population of the three sites, with few exceptions, was submitted to fecal tests using the kato-katz method and immediate intradermal tests for schistosomiasis in both studies. subjects with positive results in one of these tests were clinically evaluated by a physical examination. in 1993, the total of 827 subjects were submitted to fecal examination and 826 to intradermal test. schistosoma mansoni eggs were found in the feces of 154 (18.6%) subjects, while 478 (57.9%) subjects presented a positive intradermal test. stool examination was carried out in 367 subjects in alegre with a positivity rate of 14.9%; the intradermal test, performed in 366 subjects, was positive in 47.5% of the cases. in alian?a, 277 subjects had their feces examined and were submitted to an intradermal test, with a positivity rate of 34.4% and 70.7%, respectively. finally in coroatá ii, 183 inhabitants submitted to fecal and intradermal tests had positivity rates of 2.2% and 59.0%, respectively. when the present data were compared to those obtained in the survey performed in 1987, a significant decrease in the prevalence of infection by s. mansoni was observed in alegre and coroatá ii, and a prevalence increase in alian?a.
SCHISTOSOMIASIS MANSONI IN THE "BAIXADA OCIDENTAL MARANHENSE", STATE OF MARANH O, BRAZIL: CROSS-SECTIONAL STUDIES PERFORMED IN 1987 AND 1993  [cached]
CUTRIM Raimundo Nonato Martins,CHIEFFI Pedro Paulo,MORAES José Cássio de
Revista do Instituto de Medicina Tropical de S?o Paulo , 1998,
Abstract: A cross-sectional study on the prevalence of schistosomiasis mansoni in three sites of the "Baixada Ocidental Maranhense" was carried out in 1993 in: Alegre (in the municipality of S o Bento), Alian a (in Cururupu) and Coroatá II (in the municipality of S o Jo o Batista). Results were compared to those of another study performed at the same sites and in similar conditions, in 1987. The entire population of the three sites, with few exceptions, was submitted to fecal tests using the Kato-Katz method and immediate intradermal tests for schistosomiasis in both studies. Subjects with positive results in one of these tests were clinically evaluated by a physical examination. In 1993, the total of 827 subjects were submitted to fecal examination and 826 to intradermal test. Schistosoma mansoni eggs were found in the feces of 154 (18.6%) subjects, while 478 (57.9%) subjects presented a positive intradermal test. Stool examination was carried out in 367 subjects in Alegre with a positivity rate of 14.9%; the intradermal test, performed in 366 subjects, was positive in 47.5% of the cases. In Alian a, 277 subjects had their feces examined and were submitted to an intradermal test, with a positivity rate of 34.4% and 70.7%, respectively. Finally in Coroatá II, 183 inhabitants submitted to fecal and intradermal tests had positivity rates of 2.2% and 59.0%, respectively. When the present data were compared to those obtained in the survey performed in 1987, a significant decrease in the prevalence of infection by S. mansoni was observed in Alegre and Coroatá II, and a prevalence increase in Alian a.
Isolamento de esporos de Paenibacillus larvae subsp. larvae no Brasil  [cached]
Schuch Dulce Maria Tocchetto,Tochetto Lisane Goldmeier,Sattler Aroni
Pesquisa Agropecuária Brasileira , 2003,
Abstract: Este trabalho objetivou detectar presen a de esporos de Paenibacillus larvae subsp. larvae em produtos de um entreposto do interior do Estado do Rio Grande do Sul, a identifica o de possíveis fontes de contamina o e a avalia o da possibilidade da transferência de esporos para colméias de apiários adjacentes a partir de produtos importados contaminados. Foram analisados mel e pólen importados disponíveis no entreposto, favo do ninho (crias, pólen e mel) colhido de uma colméia sadia, mel estocado em um dos apiários e abelhas adultas. Os resultados foram positivosem rela o ao mel e pólen importados, a três grupos de abelhas adultas e ao mel do favo.
ESTUDO DA NORMALIDADE DA PLUVIOMETRIA NO SETOR SERRANO DE CUBAT O E BAIXADA SANTISTA - SP  [cached]
Luci Hidalgo Nunes
Revista do Departamento de Geografia , 1992, DOI: 10.7154/rdg.v0i6.246
Abstract: ESTUDO DA NORMALIDADE DA PLUVIOMETRIA NO SETOR SERRANO DE CUBAT O E BAIXADA SANTISTA - SP
Suscetibilidade de Biomphalaria tenagophila do Estado de S?o Paulo à infec??o por linhagens de Schistosoma mansoni da Baixada Maranhense (Maranh?o, Brasil)
Bastos,Othon de Carvalho; Schiavotelo,Rosimary de Jesus Gomes; Ribeiro,Maria Lúcia de Jesus Filgueiras;
Revista de Saúde Pública , 1984, DOI: 10.1590/S0034-89101984000500004
Abstract: wild (w) and human (h) strains of schistosoma mansoni were isolated in the lowland region of maranh?o state (northeastern brazil). snail progenies of biomphalaria tenagophila collected from paraiba do sul river valley (southeastern brazil) were exposed to w miracidia, obtained from livers of wild rodents,and to h miracidia, from eggs in human stools. the date of elimination of cercariae, the quantity of eliminated larvae, the infection index of the moluscs and the mortality rate of the snails were recorded. this data suggest better adaptation of the w strain than of the h strain to b. tenagophila. these results were compared with published data of h and w strains from the paraiba do sul river valley and similar parasitological behavior was found.
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