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Molecular studies on the Creole cattle breed in Mauritius  [PDF]
Olivier Pasnin, V. M. Ranghoo-Sanmukhiya
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.410121
Abstract:

There are three main cattle breeds in Mauritius; the Friesian cows, the Creole cows and the Cross (Creole and Friesians) breeds. The main objective of the study was to differentiate the Creole breeds from the other two breeds thus valorizing and conserving the Creole cow which is in danger of extinction. Random Amplified Polymorphic DNA and random microsatellite analysis were the two PCR based techniques used. The populations studied consisted of 5 Friesians, 5 Creole breeds and 5 Cross breeds. The breeds were obtained from the Curepipe Livestock Research Station which is the only place where there is a record of Creole cows in Mauritius. Among the 5 Creoles breeds chosen, 2 of them could have been impure breeds due to their morphological characteristics. DNA extraction was carried out from blood taken from the cows selected, and yielded DNA of good quality and quantity. Polymorphic bands were obtained from the Random Amplified Polymorphic DNA primers and random microsatellite primers and the data obtained were used for constructing a dendogram. From the dendogram obtained, the breeds were separated and the two Creole samples, which were suspected to be impure, formed different clusters from the true Creole breeds. From the results obtained, the Creole breed was easily distinguishable from the other breeds studied using molecular techniques. 

Molecular genetic analysis of a cattle population to reconstitute the extinct Algarvia breed
Catarina Ginja, Maria CT Penedo, Maria F Sobral, José Matos, Carla Borges, Dina Neves, Teresa Rangel-Figueiredo, Alfredo Cravador
Genetics Selection Evolution , 2010, DOI: 10.1186/1297-9686-42-18
Abstract: 46 individuals phenotypically representative of Algarvia cattle were genotyped for 27 microsatellite loci and compared with 11 Portuguese autochthonous and three imported breeds. Genetic distances and factorial correspondence analyses (FCA) were performed to investigate the relationship among Algarvia and related breeds. Assignment tests were done to identify representative individuals of the breed. Y chromosome and mtDNA analyses were used to further characterize Algarvia animals. Gene- and allelic-based conservation analyses were used to determine breed contributions to overall genetic diversity.Genetic distance and FCA results confirmed the close relationship between Algarvia and southern Portuguese breeds. Assignment tests without breed information classified 17 Algarvia animals in this cluster with a high probability (q > 0.95). With breed information, 30 cows and three bulls were identified (q > 0.95) that could be used to reconstitute the Algarvia breed. Molecular and morphological results were concordant. These animals showed intermediate levels of genetic diversity (MNA = 6.0 ± 1.6, Rt = 5.7 ± 1.4, Ho = 0.63 ± 0.19 and He = 0.69 ± 0.10) relative to other Portuguese breeds. Evidence of inbreeding was also detected (Fis = 0.083, P < 0.001). The four Algarvia bulls had Y-haplotypes H6Y2 and H11Y2, common in Portuguese cattle. The mtDNA composition showed prevalence of T3 matrilines and presence of the African-derived T1a haplogroup. This analysis confirmed the genetic proximity of Algarvia and Garvonesa breeds (Fst = 0.028, P > 0.05). Algarvia cattle provide an intermediate contribution (CB = 6.18, CW = -0.06 and D1 = 0.50) to the overall gene diversity of Portuguese cattle. Algarvia and seven other autochthonous breeds made no contribution to the overall allelic diversity.Molecular analyses complemented previous morphological findings to identify 33 animals that can be considered remnants of the Algarvia breed. Results of genetic diversity and conservation an
GENETIC VARIABILITY OF THE ZEBU CATTLE BREED (Bos indicus) IN THE DEPARTAMENT OF HUILA, COLOMBIA USING MICROSATELLITE MOLECULAR MARKERS
HERNáNDEZ ESCOBAR,CARLOS; OLIVERA áNGEL,MARTHA; OSTOS ALFONSO,HENRY; GUERRA,MARIA TERESA;
Acta Biológica Colombiana , 2009,
Abstract: the polymorphism of 11 microsatellites from zebu cattle (bos indicus) was studied using a commercial multiplex system to estimate genetic variability. allele frequencies polymorphism information content and heterozygosis were calculated. allele frequencies revealed that in the analyzed sample the markers were not equally polymorphic. the average allele was 14.2 with the highest values for the tgla122 microsatellites. the mean heterozygocity was 0.7056 and the polymorphism information content was 0.668. this multiplex analysis could be used for pedigree information and for adequate genetic improvements in breeding programs and paternity test.
Genetic variability of the zebu cattle breed (Bos indicus) in the departament of Huila using microsatellite molecular markers  [cached]
Hernandez-Escobar carlos
Acta Biológica Colombiana , 2009,
Abstract: The polymorphism of 11 microsatellites from Zebu cattle (Bos indicus) were studied using a commercial multiplex system, to estimate genetic variability. Allele frequencies, polymorphism information content and heterozygosis were calculated. Allele frequencies revealed that in the sample analyzed the markers were not equally polymorphic. The average allele was 14.2 with the highest values for the TGLA122 microsatellites. The mean heterozygocity was 0.7056 and the polymorphism information content was 0.668. This multiplex analysis could be used for pedigree information and for adequate genetic improvements in breeding programs.
Genetic variability of Pantaneiro horse using RAPD-PCR markers
Egito, Andréa Alves do;Fuck, Beatriz Helena;McManus, Concepta;Paiva, Samuel Rezende;Albuquerque, Maria do Socorro Maués;Santos, Sandra Aparecida;Abreu, Urbano Gomes Pinto de;Silva, Joaquim Augusto da;Sereno, Fabiana Tavares Pires de Souza;Mariante, Arthur da Silva;
Revista Brasileira de Zootecnia , 2007, DOI: 10.1590/S1516-35982007000400007
Abstract: blood samples were collected from pantaneiro horses in five regions of mato grosso do sul and mato grosso states. arabian, mangalarga marchador and thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by rapd-pcr (random amplified polymorphic dna - polymerase chain reaction) molecular markers. from 146 primers, 13 were chosen for amplification and 44 polymorphic bands were generated. the analysis of molecular variance (amova) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75.47%). analysis of the genetic variability between pairs of populations presented higher estimates for the five pantaneiro populations with the arabian breed, while lowest estimates were presented by pairs formed among the pantaneiro populations with the mangalarga marchador. highest genic diversity was shown by the pantaneiro (0.3396), which also showed highest genetic distance with the arabian and lowest with mangalarga marchador breed. upgma dendrogram showed distinct differences between naturalized (pantaneiro and mangalarga marchador) and exotic (arabian and thoroughbred) breeds. in the dendrogram generated by upgma method, the similarity matrix generated by the jaccard coefficient showed distinction between the naturalised breeds, pantaneiro and mangalarga marchador, and the exotic breeds, árab and english thoroughbred. results suggest that the pantaneiro presents a higher genetic variability than the other studied breeds and has a close relationship with the mangalarga marchador.
Cholistan and Cholistani Breed of Cattle  [PDF]
U. Farooq, H. A. Samad*, F. Sher1, M. Asim1 and M. Arif Khan2
Pakistan Veterinary Journal , 2010,
Abstract: Cholistan, once a green and prosperous land with the source of water being the ancient Hakra River, was also the cradle of great Hakra Valley Civilization. It is sprawled at an area of 26,000 Km2, located between the latitudes 27o42′and 29o45′North and longitudes 69o52′and 75o24′East. The bioclimatic system of Cholistan falls under the category of “tropical desert” with very scanty rainfall. Geomorphologically, the soils of Cholistan are a complex blend of river alluvium and Aeolin sands. Based on topography, type of soil and vegetation, this desert is divided into two geomorphic regions: the Lesser Cholistan (the Northern 7,770 Km2 region) and the Greater Cholistan (the Southern 18,130 Km2 region). The primary source of water is rainfall which is utilized through natural depressions or man-made ponds called “Tobas” and “Dahars.” The secondary source is underground water which is brackish and salty and not fit for human/animal consumption. Two livestock production systems prevail under pastoralism in Cholistan viz. transhumanie and nomadic. Despite an uncertain, unpredictable rainfall, low humidity and extremes in temperatures, Cholistan has long been famous for raising different breeds of livestock, contributing a significant share to national milk, meat and wool output. The total livestock population estimated during 2006 was 12,09528, out of which 47% were cattle. Cholistani cattle are considered to be ancestor of the Sahiwal and are a thermo-tolerant, tick-resistant breed. Preliminary data on some productive and reproductive traits of Cholistani cows maintained at Govt. Livestock Station, Jugait Peer, Bahawalpur during the period 2005 to 2009 revealed the avergae values for the productive traits i.e. lactation length, lactation yield, dry period, service period and fat percentage in milk as 165 days, 1235 liters, 155 days, 121 days and 4.8%, respectively. Similarly, the average values for reproductive traits i.e. age at maturity, age at first calving, gestation period and calving interval were 1112, 1390, 278 and 422 days, respectively, which are at par with those of Sahiwal and Red Sindhi cattle. However, comprehensive data supported by research need to be analyzed to manipulate the potentials of this breed. Furthermore, gene mapping and Marker Assisted Selection (MAS) will open up new horizons to study performance traits of Cholistani cattle in detail.
Estudio anatomopatológico, citogenético y molecular del síndrome freemartin en el bovino doméstico (Bos taurus) - (anatomopathologic, cytogenetic and molecular studies of the freemartin syndrome in cattle (bos taurus))  [PDF]
Ayala-Valdovinos, Miguel Angel,Villagómez, Daniel,Galindo-García, Jorge,Sánchez-Chiprés, David
REDVET , 2007,
Abstract: ResumenA través del análisis citogenético utilizando la presencia de los cromosomas sexuales como marcadores y mediante el análisis molecular (PCR-RFLP), utilizando oligonucleótidos iniciadores para los genes Zfx y Zfy del bovino, se diagnosticaron con la condición intersexual, síndrome freemartin, 19 individuos (Bos taurus) procedentes de partos múltiples heterosexuales; asimismo, se estudiaron anatomopatológicamente cuatro de estos animales, enfatizando en las características del tejido gonadal (ovárico y/o testicular) presente, procediendo a la identificación molecular (PCR-RFLP) de células masculinas en este tejido. El quimerismo cromosómico hematopoyético (60,XX/60,XY) caracterizó a los animales estudiados, excepcionalmente un individuo mostró complemento cromosómico 60,XY. Mediante la técnica de PCR, se amplificó un fragmento de 447 pb, del cual después de ser digerido con la enzima de restricción Pst I, se obtuvieron los RFLP de los animales estudiados, hembras normales (447 pb) así como machos normales y animales freemartins (447, 344 y 103 pb). Los hallazgos anatomopatológicos de dos de los cuatro freemartins estudiados hasta su sacrificio mostraron pseudohermafroditismo masculino, de estos dos intersexos además se obtuvo ADN de tejido gonadal, del cual se identificó a nivel molecular (PCR) la presencia de un patrón de RFLP específicos de freemartin. Los dos freemartins restantes presentaron una marcada hipoplasia del tracto reproductor sin gónadas diferenciadas como tales. AbstractBy means of the cytogenetic analysis using the presence of the sexual chromosomes as markers and through the molecular analysis (pcr-rflp), using primers for the zfx and zfy genes of the bovine, 19 individuals (bos taurus) coming from heterosexual multiple parturitions, were diagnosed as syndrome freemartin intersexes; likewise, there were studied anatomopathologically four of these animals, emphasizing the characteristics of the gonadal tissue (ovaric and/or testicular) present, proceeding to the molecular identification (pcr-rflp) of masculine cells in this tissue. the presence of hematopoyetic chromosomic chimerism (60,xx/60,xy) characterized to the animals studied, exceptionally a freemartin showed chromosomic complement 60,xy. by means of pcr's technique, a fragment of 447 bp was amplified, and digested with pst i restriction enzyme, the rflp of the studied animals was obtained, normal females (447 bp) as well as normal males and freemartin animals (447, 344 and 103 bp). the anatomopathologically findings of two of the four freemartins studied at slaughter showe
A Cytogenetic Study on the Angora Breed of Goat (Capra hircus) Reared in Turkiye  [PDF]
D. Nicodemo,A. Pauciullo,A. Castello,M.I. Soysal
Journal of Tekirdag Agricultural Faculty , 2008,
Abstract: The Angora breed of goat plays an important role for the Turkiye's Native Animal genetic resources point of view; nevertheless, so far, no comprehensive cytogenetic investigation has been performed on this important economic breed. The present preliminary cytogenetic study has been conducted upon a sample of Angora goat breed reared in Turkiye in order to ascertain the basic chromosomal status of the breed and to start a cytogenetic screening at a population level. Blood cultures have been noted according to the conventional methods and synchronized with thymidine. Conventional and GTG-RBA-RBG-banded karyotypes have been produced to determine the relative length and centromeric index of the chromosomes of the breed. Banding comparison confirmed similarity of the Angora breed of goat with the established standardized RBA-RBG banding a pattern of the goat species. Further cytogenetic studies should now be addressed at a population level to identify carriers of numerical and/or structural chromosomal abnormalities in the Angora breed population for implementation of its productive and reproductive efficiency.
Haematology of Sokoto Gudali Cattle as Influenced by Sex and Breed
F.O. Olayemi,C.N. Nwandu,J.O. Aiyedun
Journal of Animal and Veterinary Advances , 2012,
Abstract: The effects of sex and breed on the haematological parameters were determined in the Sokoto Gudali breed of cattle. The Sokoto Gudali cattle had significantly higher Haemoglobin concentration (Hb) (p< 0.01) and Mean Corpuscular Haemoglobin Concentration (MCHC) (p< 0.001) than the White Fulani cattle. However, the values of mean Corpuscular Haemoglobin (MCH), Mean Corpuscular Volume (MCV) and Packed Cell Volume (PCV) and the Red Blood Cell (RBC), total White Blood Cell (WBC), neutrophil, lymphocyte, eosinophil and monocyte counts were similar in the two breeds of cattle. There were no sexual dimorphism (p>0.05) in the mean values of Hb concentration, MCH, MCHC, MCV and PCV of the Sokoto Gudali cattle. Also, the RBC, WBC, neutrophil, lymphocyte, eosinophil and monocyte counts were similar in the male and female.
Conservation genetics of an endangered Catalonian cattle breed ("Alberes")
Jordana, J.;Piedrafita, J.;Carre, X.;Martell, A.;
Genetics and Molecular Biology , 1999, DOI: 10.1590/S1415-47571999000300017
Abstract: we biochemically analyzed and characterized the genetic structure of a population in danger of extinction ,"alberes", a local cattle breed of the catalonian pyrenees (spain and france). ninety-two individuals were analyzed for five polymorphic genetic loci (hb, alb, tf, gc and ptf2). the animals were grouped according to coat color: fagina alberes variety (n = 39) and black alberes variety (n = 53). the genetic structures and relationships between these subpopulations and one "outgroup" breed ("bruna dels pirineus") were analyzed and compared by using f-statistics. we determined that inbreeding in the alberes breed is not significant, since negative and nonsignificant fit and fis values were obtained. the average genetic differentiation between subpopulations within the alberes breed was 1.5% (fst = 0.015; p < 0.05), with an effective number of 4.1 individuals exchanged between subpopulations per generation (gene flow). the results obtained in this study corroborate the potential danger of extinction of the breed. the black alberes variety is proposed as the principal nucleus of genetic conservation for this breed, as it seems to show a higher degree of genetic isolation from other foreign populations.
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