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Shading and periods of acclimatization of micropropagated banana plantlets cv. Grande Naine
Scaranari, Ciro;Leal, Paulo Ademar Martins;Mazzafera, Paulo;
Scientia Agricola , 2009, DOI: 10.1590/S0103-90162009000300008
Abstract: banana plantlets obtained by micropropagation need to be submitted to a period of acclimatization since they do not use light, water, and nutrients in an efficient way. the acclimatization must be carried out under greenhouse conditions where temperature, light, and air humidity are adequate for a gradual hardening of the plantlets. in this study, the development of banana plantlets was evaluated during acclimatization under a full light condition including covered surfaces with red shade cloth (70%, 50%, and 30% shade) and black shade cloth (50% shade), both under a transparent plastic film of 100 μm. temperature, relative air humidity, irrigation, and nutrition conditions were also controlled. physical and physiological parameters were recorded at various stages in the greenhouses after three, six, and nine weeks and also after seven weeks of transplanting to field conditions. treatments were hierarchically graded according to their statistic classification. combined results indicated superior outcomes of plantlets maintained under black 50% shade cloth for nine weeks, both in the summer and winter seasons. similar results, but in a shorter time, were obtained with plantlets cultivated under red 70% shade cloth, for six weeks in the summer.
Substratos e fungo micorrízico arbuscular em mudas micropropagadas de bananeira na fase de aclimata??o
Trindade, Aldo Vilar;Lins, Gêlva Maria de Lima;Maia, Isabel Cristina Silva;
Revista Brasileira de Fruticultura , 2003, DOI: 10.1590/S0100-29452003000100039
Abstract: with the aim of studying different substrates and the arbuscular mycorrhizal fungus gigaspora margarita in the growth of micropropagated banana plants it was conducted an experiment under greenhouse conditions at the campo-cpa (micropropagating plant)/embrapa-brazilian agricultural research corporation, in the city of cruz das almas, state of bahia. thirteen substrates were tested with and without inoculation with the arbuscular mycorrhizal fungus, during the acclimatization phase. the plants were inoculated at the time of transplanting to the different substrates and were collected after the acclimatization period of 55 days, for evaluation of its growth, mineral nutrition and mycorrhizal colonization. the arbuscular mycorrhizal fungus g. margarita colonized well and increased the growth of the micropropagated banana plants, depending on the substrate used; the substrate turfgrass + vermiculite + 5% manure promoted the formation of normal and healthy plants but only in the presence of the mycorrhizal fungus; the use of the commercial substrate rendmax citrus gave the best plant growth but inhibited mycorrhizal colonization and effects; addition of nutrients to rendmax citrus was not necessary for banana plantlets; vermicompost was a good substrate for banana plantlet associated with am inoculation.
Morpho-physiological changes in pineapple plantlets [Ananas comosus (L.) merr.] during acclimatization Mudan as morfofisiológicas em plantulas de abacaxi [Ananas comosus (L.) Merr.] durante a aclimatiza o  [cached]
Ariel Villalobo,Justo González,Ramón Santos,Romelio Rodríguez
Ciência e Agrotecnologia , 2012, DOI: 10.1590/s1413-70542012000600004
Abstract: Pineapple producing countries lack good quality propagation material to expand cultivars into new areas. Previously, research protocols may increase the offer of high quality plantlets and speed the introduction of new pineapple cultivars. The present work is to evaluate the morpho-physiological changes in plantlets of pineapple [Ananas comosus (L.) Merr. 'MD-2'] during the acclimatization phase. Plantlets were acclimatized under 80% relative humidity, 25.5 °C temperature and photosynthetic photon flux of 400-500 μmol m-2 s-1 as average for 45 d under natural photoperiods. All measurements (plant length, number of leaves and roots, fresh weight, width and length of leaf 'D', net photosynthesis and total transpiration rate) were carried out at the end of in vitro rooting phase coincident with 0 d of acclimatization and at 15, 30 and 45 d thereafter. Photosynthetic activity of in vitro plantlets did not increase during the first 30 d of the acclimatization phase. After 30 d, photosynthetic activity ranged from 5.72 to 9.36 μmol CO2 m-2 s-1 while total transpiration ranged from 6.0 to 1.42 mmol H2O m-2 s-1. During the first 30 days there were no significant differences in number of leaves, length or width of the longest ('D') leaf (cm) or plant length (cm). However, after 45 days plant fresh weight (g), length and width of the 'D' leaf (cm) and root number all increased significantly, while transpiration (mmol H2O m-2 s-1) declined. There were small but significant decreases in chlorophyll a and b (μg g-1 mf.). Increased photosynthetic activity after 30 d shows that the increase in light intensity and the reduction of relative humidity during acclimatization did not constitute inhibitory factors. Os países produtores do abacaxi precisam de mudas de boa qualidade para a introdu o de novas culturas nas áreas de cultivo. Os protocolos de pesquisa podem aumentar a oferta de mudas de alta qualidade e acelerar a introdu o de novas culturas do abacaxi. O estudo apresentado teve como objetivo avaliar as altera es morfofisiológicas de mudas de abacaxi [Ananas comosus (L.) Merr. 'MD-2'], durante a fase de aclimata o. As plantas foram aclimatizadas baseadas em um regímen de 80% de humidade relativa, uma temperatura de 25°C e um fluxo médio de fótones fotossinteticamente ativos de 400-500 μmol m-2 s-1, durante 45 dias em condi es naturais de ilumina o. As medi es todas (comprimento da planta, número de folhas, comprimento, largura da folha maior 'D', taxa fossintetica e taxa transpiratória total) foram feitas no final da fase do enraizamento in vitro a coincidir
The effects of different indole-3-butyric acid (IBA) concentrations, two light regimes of in vitro rooting and acclimatization of in vitro teak (Tectona grandis L.f) plantlets
AS Nor Aini, BL Goh, R Ridzuan
African Journal of Biotechnology , 2009,
Abstract: Effects of different indole-3-butyric (IBA) concentrations (0, 0.5, 1.0, 2.0, 3.0 and 5.0 mg/l), two light regimes of in vitro rooting and acclimatization on in vitro teak (Tectona grandis L.f) were investigated. Shoots incubated in the light produced higher mean number of roots (2.0) and mean root length of 15.0 and 4.5 mm when supplemented with low IBA concentrations of 0.5 and 1.0 mg/l, respectively. Conversely, when shoots were incubated in the dark, higher mean number of roots (8.0 and 3.0) and mean root lengths (14.0 and 8.8 mm) were produced when supplemented with higher IBA concentrations of 2.0 and 3.0 mg/l, respectively. IBA was found necessary for root induction and growth in light and dark where 2.0 mg/l was optimum as it recorded high mean number of root (6.0 and 8.0) with mean root lengths of 11.8 and 14.0 mm, respectively. High survival percentage of 80% was recorded for those plantlets raised in “jiffy-7” but survival reduced to 40% when grown in the autoclaved soil after one week.
Optimization of acclimatization system for embryo rescue tube plantlets of seedless grape

- , 2015,
Abstract: 【目的】研究炼苗移栽过程中生根粉溶液浸根时间、营养液处理、开始通风时间及遮阴处理对无核葡萄胚挽救苗炼苗及移栽的影响,探索胚挽救试管苗炼苗移栽技术。【方法】以2011年与2012年无核葡萄胚挽救杂交育种后代试管苗为材料,研究生根粉溶液浸根时间、炼苗过程中是否添加营养液、揭开保湿罩时间以及移栽大田过程中遮阴对胚挽救苗炼苗及移栽的影响。【结果】1.0 mg/L生根粉溶液浸泡根系3 min时植株各项生理指标较好且成活率最高;移栽过程中添加1/8 MS营养液一定程度上抑制了植株的长势;炼苗初期保湿时间为4 d时炼苗成活率最高;移栽至温室或大田中时一定程度的遮阴处理有利于移栽成活率的提高与植株的生长发育。【结论】无核葡萄胚挽救试管苗炼苗前生根粉浸根处理3 min、炼苗时添加1/8 MS营养液、炼苗后4 d开始通风以及移栽时进行遮阴能提高胚挽救试管苗的生物积累量和成活率。
【Objective】Effects of ABT soak time,nutrition,lid off period and shade treatment on embryo rescue seedling and transplantation were studied to improve the embryo rescue seedling transplant technique.【Method】The hybrid progeny tube plantlets of seedless grape through embryo rescue in 2011 and 2012 were selected to study the effects of ABT soak time,nutrition,lid off period and shade treatment on growth and survival rate of embryo rescue seedling in domestication.【Result】The survival rate and growth of plants were best with 1.0 mg/L ABT solution soaking for 3 min.The growth of tube plantlets was inhibited after irrigating 1/8 MS nutrient solution.Survival rate of domestication was highest with lid off 4 days after hardening.Shade treatment after transplant promoted growth and improved survival rate.【Conclusion】Bioaccumulation and survival rate of seedless grape embryo rescue tube plantlets were highest when soaking root in ABT for 3 min,adding 1/8 MS nutrient solution during hardening,and starting ventilation 4 d after hardening with shade treatment
Acclimatization of in Vitro Propagated Pineapple (Ananas comosuss (L.), var. Smooth cayenne) Plantlets to ex Vitro Condition in Ethiopia  [PDF]
Ayelign Mengesha, Biruk Ayenew, Tewodros Tadesse
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.42042
Abstract: Pineapple (Ananas comosuss, var. Smooth cayenne), which is a popular tropical fruit, is propagated vegetatively. Conventional propagation alone does not provide clean and adequate planting material demanded in Ethiopia. Recently, in vitro multiplication has become a promising technique for large-scale production. However, the acclimatization to the external environment procedure impedes the efficiency, which needs carefully optimized acclimatization techniques. We report optimized acclimatization procedures following first- and second-stage hardening methods for in vitro pineapple plantlets. Primarily, Jiffy-7 peat pellet allowed growing plants vigorously and provided above 8% survival rate over soil mix. Nevertheless, in Ethiopia, soil mix is cheaper and locally accessible. The primarily acclimatized plantlets are needed to be hardened further for better establishment and survival in the field. Black polybag and polysleeve pots filled with soil mix were evaluated in the greenhouse. A significant difference was obtained between pots for number of roots and substrate weight. Polybags had higher root number than polysleeves and saved about 27% of substrates per plant, which is a reduction of 25% of total transportation cost. Hence, the soil mix and polybags were found to be preferable over substrates and pots, for subsequent in vitro pineapple acclimatization.
Influence of Plantlet Age and Different Soilless Culture on Acclimatization of Stemona curtisii Hook.f.  [PDF]
Jiraporn Palee,Srisulak Dheeranupattana,Araya Jatisatienr,Sunanta Wangkarn
Asian Journal of Plant Sciences , 2012,
Abstract: The aim of this experiment was to study the optimal age of Stemona curtisii plantlets for acclimatization. The in vitro shoots of S. curtisii were cultured on Murashige and Skoog solid medium supplemented with 1 mg L-1 naphthalene acetic acid to induce roots. Then, the plantlets from in vitro culture of 4, 8 and 12 week-old were transferred into the soil and their survival rate during the acclimatization process in the greenhouse was investigated. It was found that the 8 week-old plantlets had the highest survival rate of 80%. However, to enhance the survival rate of these plantlets, a soilless culture technique as a possible approach for the acclimatization was considered. Eight week-old plantlets were transferred into either, soil, a hydroponic system (nutrient film technique), coconut fiber or sand. The plantlets which were cultivated in coconut fiber or hydroponic culture showed 100% survival rate with the highest average number of new roots per plant. However, the mean root length of the plantlets grown in hydroponic system was significantly higher than that grown in coconut fiber.
Scientific Annals of Alexandru Ioan Cuza University of Iasi. New Series, Section 2. Vegetal Biology , 2008,
Abstract: In micropropagation, the success of cloning depends on the surviving process of the exvitroplantlets after the acclimatization and on the quality of the resulting planting material. For the planting material, coming from vitroculture, to be competitive with the one obtained through classic vegetative multiplying, especially for African violets, it has to be cheaper and better quality. So, the ex vitro” planting of the African violet plantlets, directly into greenhouse conditions, is possible and favorable if the substratum in which the acclimatization is being made is efficient and low cost. From the seven types of substratum we tested for exvitroplantlets acclimatization, the most efficient, regarding the post acclimatization survival percent, was 100%, the Top soil” substratum, a worm compost, the exvitroplantlets growth spores set on this type of substratum having a very good rooting, these data being meaningful statistically speaking.
Associa??o de fungos micorrízicos arbusculares e cultivares micropropagadas de antúrio
Stancato, Giulio Cesare;Silveira, Adriana Parada Dias da;
Bragantia , 2006, DOI: 10.1590/S0006-87052006000300018
Abstract: one of the most important steps on micropropagated anthurium plantlets is the acclimatization. mycorrhization could be a process that helps the plantlets to change to the autotrophic state. the objective was to evaluate the effect of the mycorrhizal association on the growth of anthurium cultivars. a experiment was conducted under greenhouse conditions, using the cultivars iac astral, iac eidibel, iac juréia, iac luau, iac netuno and iac ?mega and the arbuscular mycorrhizal fungi glomus intraradices, glomus etunicatum and acaulospora sp., in organic substrate, at the plantlets acclimatization stage. the results showed that there was increase in shoot dry matter in mycorrhizal plantlets and that the symbiotic efficiency varied according to the cultivar and arbuscular mycorrhizal fungus.
Population genetics of Drosophila ananassae: Chromosomal association studies in Indian populations  [PDF]
Singh Pranveer,Singh B.N.
Genetika , 2010, DOI: 10.2298/gensr1002210s
Abstract: Forty-five natural populations of Drosophila ananassae and laboratory stocks made from these flies were analyzed for chromosome inversions. Quantitative data on the frequencies of these inversions were utilized to test intra- and interchromosomal interactions in D. ananassae. In most of the natural as well as laboratory populations no significant deviation from randomness of intra- and interchromosomal associations (2L-3L, 2L-3R, 3L-3R) was found hence, providing evidence for random associations. However, in some instances, significant deviation from randomness was found in both natural and laboratory populations, which could be due to excess of certain combinations, deficiency of others and complete absence of some combinations. Possible role of genetic drift could be implicated due to tight-linkage between linked gene arrangements. This strengthens the previous suggestion that there is lack of genetic coadaptation in D. ananassae.
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