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Evaluation of Bifenthrin and Acorus calamus Linn. Extract against Aedes aegypti L. and Aedes albopictus (Skuse)
S Sulaiman,DSF Abang Kamarudin,H Othman
Iranian Journal of Arthropod-Borne Diseases , 2008,
Abstract: "nBackground: Bifenthrin and Acorus calamus Linn extract were evaluated against dengue vectors in the laboratory."nMethods: Both Bifenthrin and Acorus calamus Linn crude hexane extract were bioassayed against the adults and larval stages of dengue vectors Aedes aegypti L. and Aedes albopictus(Skuse) in the laboratory."nResults: The A. calamus crude hexane extract exhibited a larvicidal activity against 4th-instar Ae. aegypti larvae with LC50 and LC90 values of 0.4418 and 11.3935 ppm respectively. The plant crude extract exhibited against Ae. albopictus larvae with a higher LC50 and LC90 values of 21.2555 ppm and 36.1061 ppm, respectively. There was a significant difference on the effect of A. calamus extract on both Aedes spp. Larvae (P< 0.05). However, bifenthrin showed a significant difference on larvicidal effect to that of A. calamus hexane extract on both Aedes spp (P< 0.05). In testing the adulticidal activity, this plant extract exhibited the LC50 and LC90 values of 17.4075 and 252.9458 ppm against Ae .aegypti and a higher LC50 and LC90 values of 43.9952 and 446.1365 ppm respectively on Ae. albopictus. There was no significant difference on the effect of A. calamus extract on both Aedes spp adults (P> 0.05)."nConclusion: Bifenthrin however showed a significant difference on both Aedes spp adults (P< 0.05). With the wide availability of A. calamus in Malaysia, it could be utilized for controlling dengue vectors. "n
Evaluation of Electronic Mosquito-Repelling Devices Using Aedes albopictus (Skuse) (Diptera: Culicidae)
Neotropical Entomology , 2001, DOI: 10.1590/S1519-566X2001000300030
Abstract: the mosquito-repelling devices anti-pic?, mosquito repeller? dx-600 and bye-bye mosquito? were evaluated in boxes for experimentation by exposing human hands to aedes albopictus (skuse) adults. two sets of tests were performed based on 15 min. expositions. in the first set both hands were introduced in the box, one of them holding the device on. in the second set only one hand was introduced each time, holdind the device on or off. the devices failed to show efficiency in both evaluations. a seemingly 30.3% repellency due to anti-pic? in the first set of experiments was not confirmed in the second set. it was discussed the value of such devices for dengue prevention.
piggybac- and PhiC31-Mediated Genetic Transformation of the Asian Tiger Mosquito, Aedes albopictus (Skuse)  [PDF]
Geneviève M. C. Labbé,Derric D. Nimmo,Luke Alphey
PLOS Neglected Tropical Diseases , 2010, DOI: 10.1371/journal.pntd.0000788
Abstract: Background The Asian tiger mosquito, Aedes albopictus (Skuse), is a vector of several arboviruses including dengue and chikungunya. This highly invasive species originating from Southeast Asia has travelled the world in the last 30 years and is now established in Europe, North and South America, Africa, the Middle East and the Caribbean. In the absence of vaccine or antiviral drugs, efficient mosquito control strategies are crucial. Conventional control methods have so far failed to control Ae. albopictus adequately. Methodology/Principal Findings Germline transformation of Aedes albopictus was achieved by micro-injection of embryos with a piggyBac-based transgene carrying a 3xP3-ECFP marker and an attP site, combined with piggyBac transposase mRNA and piggyBac helper plasmid. Five independent transgenic lines were established, corresponding to an estimated transformation efficiency of 2–3%. Three lines were re-injected with a second-phase plasmid carrying an attB site and a 3xP3-DsRed2 marker, combined with PhiC31 integrase mRNA. Successful site-specific integration was observed in all three lines with an estimated transformation efficiency of 2–6%. Conclusions/Significance Both piggybac- and site-specific PhiC31-mediated germline transformation of Aedes albopictus were successfully achieved. This is the first report of Ae. albopictus germline transformation and engineering, a key step towards studying and controlling this species using novel molecular techniques and genetic control strategies.
Primeiro encontro de Aedes (Stegomyia) albopictus (Skuse) no Estado do Paraná, Brasil
Sant'Ana,Adson Luís;
Revista de Saúde Pública , 1996, DOI: 10.1590/S0034-89101996000400013
Abstract: the first recorded occurrence of aedes (stegomyia) albopictus (skuse) in curitiba, paraná, south of brazil, was described. the collection was carried out by means of aspirator in human bait.
Primeiro registro de Aedes (Stegomyia) albopictus (Skuse) (Diptera, Culicidae) em Morretes, planície litoranea do Estado do Paraná, Brasil
Ribeiro, Magda Clara Vieira da Costa;Santos-Neto, Luiz Gonzaga dos;
Revista Brasileira de Zoologia , 2001, DOI: 10.1590/S0101-81752001000500031
Abstract: specimens of aedes albopictus (skuse, 1894) were caugth using human bait with electric aspirator and a modified cdc trap.
Development, longevity, gonotrophic cycle and oviposition of Aedes albopictus Skuse (Diptera: Culicidae) under cyclic temperatures
L?wenberg Neto, Peter;Navarro-Silva, Mário A.;
Neotropical Entomology , 2004, DOI: 10.1590/S1519-566X2004000100006
Abstract: the effects of cyclic temperatures on aedes albopictus skuse development, longevity, gonotrophic cycle and the number of oviposited eggs were assessed by means of laboratory experiments. the experiments were carried out with mosquitoes from registro, s?o paulo, brazil, kept in insectary for two years. the development of the insect was followed from egg to adult emergence under 25/18oc and 27/20oc and adult stage under 27/20oc, both associated with ld 12:12h. the eggs received two treatments: (a) maintenance of water volume; (b) periodical and complete change of water. blood meal was offered daily and it was interrupted after haematophagy and restarted after first oviposition. the immature development was significantly different under the temperature regimes (p < 0.05) and the increased temperatures positively affected the development speed. the combination of higher temperatures regime and periodical and complete change of water increased the eggs viability and shortened the incubation time. adult longevity was not different between males and females and the mortality was regular through the time. comparing the mosquito longevity under constant and cyclic temperatures, it is suggested that the lowest temperature of the cyclic regime is a limiting factor for mosquito survival. this fact may limit the a. albopictus distribution range to areas where the minimal temperatures are not much bellow 20oc.
Surface morphology and morphometric analysis of sensilla of Asian tiger mosquito, Aedes albopictus (Skuse): an SEM investigation  [PDF]
T. Seenivasagan,Kavita R. Sharma,Anchal Shrivastava,B.D. Parashar
Journal of Vector Borne Diseases , 2009,
Abstract: Background & objectives: The sensilla and sensory mechanism play a significant role in hostseeking and oviposition behaviour of mosquitoes, which enable them to transmit various diseases to humans. Aedes albopictus (Skuse) has emerged as a major vector of Chikungunya virus in the recent epidemics in most parts of southern India. Studies on the sensory structures of dengue vector, Aedes aegypti (Linn) are comprehensive; whereas information on the sensillary systems of Asian tiger mosquito, Ae. albopictus is inadequate. Therefore, the present study has been carried out to observe various types of sensilla located on the antenna, maxillary palp, labial palp, tarsi and ovipositor of Ae. albopictus using scanning electron microscopy. Methods: The antennae, maxillary palpi, labellum, tarsi and ovipositor of 10 different female mosquito of Ae. albopictus were fixed individually in 2.5% glutaraldehyde solution, washed twice and dehydrated with ascending grades of ethanol. Samples were cleared with xylene, air-dried, mounted on stubs, gold coated in an ion-sputtering unit and the sensilla were viewed between 5 and 10 KV using FEI Quanta 400 EDAX scanning electron microscope. ANOVA revealed significant differences in the morphometric features of various sensilla. Results: In the antenna Sensilla trichoidea are numerously distributed in all flagellar segments revealed four distinct subtypes. Two types of grooved peg sensilla were observed. Sensilla coeloconica was observed in the terminal flagellum of antenna and tarsomeres with large variation in diameter. Sensilla chaetica are distributed throughout the body and revealed greater variation in morphology and morphometric parameters. Interpretation & conclusion: The significant difference among various types of sensilla would possibly reveal their functions. The porous sensilla are olfactory and contact chemoreceptors while the aporous sensilla would play the role of mechanoreception. Sensilla coeloconica on the antenna, tarsus showed major differences with Ae. aegypti. The ovipositor sensilla revealed three types of chaetica arranged in rows but has not been reported earlier with other mosquito species.
High and Low Risk Dengue Haemorrhagic Fever Areas Affecting Key Breeding Place of Aedes aegypti (L.) and Ae. albopictus (Skuse) in Nakhon Si Thammarat, Southern Thailand  [cached]
Walailak Journal of Science and Technology , 2007, DOI: 10.2004/vol4iss1pp9-22
Abstract: This study investigated key breeding sites of Aedes aegypti (L.) and Ae. albopictus (Skuse) in high and low risk dengue haemorrhagic fever (DHF) areas. Ae. aegypti and Ae. albopictus larvae were found in 11 out of 29 types of water containers in both high and low risk DHF areas. Ae. aegypti larvae were found most in outdoor area containers in high risk DHF areas and in metal boxes in low risk DHF areas. On the other hand, Ae. albopictus larvae were found most in indoor earthen jars in low risk DHF areas and in areca containers in high risk DHF areas. The number of Ae. albopictus larvae found in the earthen jars and metal or plastic boxes in low risk areas were higher than in high risk DHF areas. Larval indices (i.e. HI and BI) in both high and low risk DHF areas were greater than 10 % and 50 %, respectively, which indicated high risks of DHF transmission.
The occurrence of Aedes (Stegomyia) aegypti (Linnaeus) and Aedes (Stegomyia) albopictus (Skuse) in a rural area in Northern Paraná, Brazil and the associated Culicidae fauna
Lopes, José;
Revista Brasileira de Zoologia , 2002, DOI: 10.1590/S0101-81752002000400012
Abstract: to verify the possible occurrence of aedes aegypti (linnaeus, 1761) and aedes albopictus (skuse, 1894) in a rural area in northern paraná, brazil, a water tank was installedon a farm, to work as an artificial breeding site. collections were carried out every 15 days, during a period of 2 years. 12,876 culicidae larvae, belonging to 10 different species, including aedes aegypti and aedes albopictus, were collected, indicating their occurrence.
Primeiro encontro de Aedes (Stegomyia) albopictus (Skuse) no Estado do Paraná, Brasil  [cached]
Sant'Ana Adson Luís
Revista de Saúde Pública , 1996,
Abstract: Foi constatada a primeira ocorrência de Aedes (Stegomyia) albopictus (Skuse) na cidade de Curitiba, Paraná. A coleta foi realizada, utilizando um sugador e isca humana como atra o.
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