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Adenosine Deaminase and Guanosine Deaminase Activities in Sera of Patients with Viral Hepatitis
Kalkan, Ahmet;Bulut, Vedat;Erel, Ozcan;Avci, Senel;Bingol, Namik Kemal;
Memórias do Instituto Oswaldo Cruz , 1999, DOI: 10.1590/S0074-02761999000300018
Abstract: in order to investigate purin and primidin metabolism pathways in hepatitis, adenosine deaminase (ada) and guanosine deaminase (gda) activities in sera of patients with different types and manifestations of viral hepatitis disease (a, b, c, d, e, chronic, acute) were investigated and compared with the control group of healthy individuals. hepatitis cases were classified with respect to their serological findings and clinics. when compared all the hepatitis cases with the controls, levels of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase enzymes, as well as ada and gda, were significantly higher than the control group (p<0.01). levels of ada and gda in hepatitis cases were determined as 26.07±11.98 iu/l and 2.37±1.91 iu/l, respectively. when compared their ada and gda levels amongst the classified hepatitis groups, there was no difference in ada levels amongst cases (p>0.05). however, gda levels in hepatitis a group were closed to the controls. increase in serum ada activities in hepatitis forms may be dependent on and reflect the increase in phagocytic activity of macrophages and maturation of t-lymphocytes, and may be valuable in monitoring in viral hepatitis cases.
Adenosine Deaminase and Guanosine Deaminase Activities in Sera of Patients with Viral Hepatitis  [cached]
Kalkan Ahmet,Bulut Vedat,Erel Ozcan,Avci Senel
Memórias do Instituto Oswaldo Cruz , 1999,
Abstract: In order to investigate purin and primidin metabolism pathways in hepatitis, adenosine deaminase (ADA) and guanosine deaminase (GDA) activities in sera of patients with different types and manifestations of viral hepatitis disease (A, B, C, D, E, chronic, acute) were investigated and compared with the control group of healthy individuals. Hepatitis cases were classified with respect to their serological findings and clinics. When compared all the hepatitis cases with the controls, levels of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase enzymes, as well as ADA and GDA, were significantly higher than the control group (p<0.01). Levels of ADA and GDA in hepatitis cases were determined as 26.07±11.98 IU/l and 2.37±1.91 IU/l, respectively. When compared their ADA and GDA levels amongst the classified hepatitis groups, there was no difference in ADA levels amongst cases (p>0.05). However, GDA levels in hepatitis A group were closed to the controls. Increase in serum ADA activities in hepatitis forms may be dependent on and reflect the increase in phagocytic activity of macrophages and maturation of T-lymphocytes, and may be valuable in monitoring in viral hepatitis cases.
Adenosine deaminase activity in serum and lymphocytes of multibacillary leprosy patients
Dayal Surabhi,Nagpal R,Jain V,Chaudhary S
Indian Journal of Dermatology, Venereology and Leprology , 1996,
Abstract: Adenosine deaminase (ADA) activity was measured in serum and lymphocytes of 25 untreated patients with multibacillary leprosy. Both serum and lymphocyte ADA levels were significantly high in multibacillary leprosy patients compared to healthy controls. The results obtained did not establish any significant relationship between ADA activity and the type of leprosy.
Changes in Adenosine Metabolism in Asthma. A Study on Adenosine, 5'-NT, Adenosine Deaminase and Its Isoenzyme Levels in Serum, Lymphocytes and Erythrocytes  [PDF]
Jitender Sharma, Bala K. Menon, Vannan K. Vijayan, Surendra K. Bansal
Open Journal of Respiratory Diseases (OJRD) , 2015, DOI: 10.4236/ojrd.2015.52004
Abstract: Background: Adenosine deaminase (ADA) and 5'-nucleotidase (5'-NT) play a crucial role in adenosine metabolism in healthy individuals. Adenosine is an inflammatory mediator of asthma. Changes in adenosine metabolism and role of ADA and 5'-NT in regulating adenosine level in asthmatics and correlation of these changes with severity of asthma are not clearly understood. Methods: In this study, we screened 5217 patients, of which 2416 were diagnosed with asthma. Further, of 2416 asthmatics, only 45 patients who strictly fulfilled the selection criteria were enrolled in the study. The patients were classified into mild, moderate and severe persistent groups; each group consisted of fifteen patients. Fifteen healthy subjects served as controls. Adenosine levels and activities of 5'-NT, total ADA, ADA1 and ADA2 in serum, lymphocytes and erythrocytes were determined. The data were analysed statistically and p < 0.05 was considered significant. Results: In asthma, adenosine levels in serum, lymphocytes and erythrocytes were found to be raised significantly. A significant reciprocal correlation existed between adenosine levels in serum, lymphocytes and erythrocytes of asthmatics and FEV1%. The 5'-nucleotidase activity in serum and lymphocytes was raised in moderate and severe persistent groups and an inverse correlation existed between 5'-nucleotidase activity and FEV1% whereas in erythrocytes it was raised only in severe persistent group and FEV1% had no correlation with the 5'-nucleotidase activity. The activities of total ADA, ADA1 and ADA2 were decreased in serum and lymphocytes of moderate and severe persistent asthmatics and a positive correlation existed between total ADA and FEV1%. In erythrocytes, total ADA activity increased in mild persistent group but remained unchanged in moderate and severe persistent groups. Conclusion: The present study suggests that adenosine levels tend to increase in serum, lymphocytes and erythrocytes with the severity of bronchial asthma. The balance between ADA and 5'-NT determines the levels of adenosine in serum and lymphocytes which may result in pathogenesis of asthma, or vice versa.
Determination of Adenosine Deaminase and Acid Alpha Naphthyl Acetate Esterase Enzyme Activity of Kilis Goats
I.S. Harem,E. Karadag Sari,M. Kocak Harem,E. Atakisi
Journal of Animal and Veterinary Advances , 2012,
Abstract: We examined alpha naphthyl acetate esterase and adenosine deaminase enzyme activity in peripheral blood of kilis goat. Our aim was to determine the percentage of ANAE positive lymphocytes and Adenosine Deaminase (ADA) measurements. The proportion of ANAE positive lymphocytes was determined to be 66%, ADA activity was determined to be 15.0±1.15 U L-1. While, T lymphocytes showed an ANAE positive reaction, the eosinophil granulocytes, neutrophil granulocytes and monocytes also, showed a positive reaction. The reaction observed in T lymphocytes was a red-brown coloration, usually 1-2 granules, but enough small granules to fill the cytoplasm were detected rarely.
Adenosine Deaminase: An Aid to Diagnose Tuberculosis  [PDF]
Salman Alrokayan
Journal of Medical Sciences , 2003,
Abstract: The present study was carried out to determination of serum Adenosine deaminase (ADA) activity in tuberculosis. ADA has two main isoenzymes, ADA-1 and ADA-2 with different optimal pH, Michaelis constants and relative substrate specificity pattern. ADA-1 isoenzyme is found in all cells, with the highest concentration found in lymphocytes and monocytes, where as ADA-2 isoenzyme appears to be found only in monocytes. ADA-2 is the predominant isoforms in tuberculous pleural effusion, counting 88% of total ADA activity, where as ADA-1 is elevated in empyema, accounting for 70% of total activity. Determination of individual ADA isoenzymes and their ratio could increase the overall diagnostic value of ADA determination in tuberculosis.
A Role for Adenosine Deaminase in Drosophila Larval Development  [PDF]
Tomas Dolezal,Eva Dolezelova,Michal Zurovec,Peter J. Bryant
PLOS Biology , 2012, DOI: 10.1371/journal.pbio.0030201
Abstract: Adenosine deaminase (ADA) is an enzyme present in all organisms that catalyzes the irreversible deamination of adenosine and deoxyadenosine to inosine and deoxyinosine. Both adenosine and deoxyadenosine are biologically active purines that can have a deep impact on cellular physiology; notably, ADA deficiency in humans causes severe combined immunodeficiency. We have established a Drosophila model to study the effects of altered adenosine levels in vivo by genetic elimination of adenosine deaminase-related growth factor-A (ADGF-A), which has ADA activity and is expressed in the gut and hematopoietic organ. Here we show that the hemocytes (blood cells) are the main regulator of adenosine in the Drosophila larva, as was speculated previously for mammals. The elevated level of adenosine in the hemolymph due to lack of ADGF-A leads to apparently inconsistent phenotypic effects: precocious metamorphic changes including differentiation of macrophage-like cells and fat body disintegration on one hand, and delay of development with block of pupariation on the other. The block of pupariation appears to involve signaling through the adenosine receptor (AdoR), but fat body disintegration, which is promoted by action of the hemocytes, seems to be independent of the AdoR. The existence of such an independent mechanism has also been suggested in mammals.
A role for adenosine deaminase in Drosophila larval development.
Dolezal Tomas,Dolezelova Eva,Zurovec Michal,Bryant Peter J
PLOS Biology , 2005,
Abstract: Adenosine deaminase (ADA) is an enzyme present in all organisms that catalyzes the irreversible deamination of adenosine and deoxyadenosine to inosine and deoxyinosine. Both adenosine and deoxyadenosine are biologically active purines that can have a deep impact on cellular physiology; notably, ADA deficiency in humans causes severe combined immunodeficiency. We have established a Drosophila model to study the effects of altered adenosine levels in vivo by genetic elimination of adenosine deaminase-related growth factor-A (ADGF-A), which has ADA activity and is expressed in the gut and hematopoietic organ. Here we show that the hemocytes (blood cells) are the main regulator of adenosine in the Drosophila larva, as was speculated previously for mammals. The elevated level of adenosine in the hemolymph due to lack of ADGF-A leads to apparently inconsistent phenotypic effects: precocious metamorphic changes including differentiation of macrophage-like cells and fat body disintegration on one hand, and delay of development with block of pupariation on the other. The block of pupariation appears to involve signaling through the adenosine receptor (AdoR), but fat body disintegration, which is promoted by action of the hemocytes, seems to be independent of the AdoR. The existence of such an independent mechanism has also been suggested in mammals.
A Role for Adenosine Deaminase in Drosophila Larval Development  [PDF]
Tomas Dolezal,Eva Dolezelova,Michal Zurovec,Peter J Bryant
PLOS Biology , 2005, DOI: 10.1371/journal.pbio.0030201
Abstract: Adenosine deaminase (ADA) is an enzyme present in all organisms that catalyzes the irreversible deamination of adenosine and deoxyadenosine to inosine and deoxyinosine. Both adenosine and deoxyadenosine are biologically active purines that can have a deep impact on cellular physiology; notably, ADA deficiency in humans causes severe combined immunodeficiency. We have established a Drosophila model to study the effects of altered adenosine levels in vivo by genetic elimination of adenosine deaminase-related growth factor-A (ADGF-A), which has ADA activity and is expressed in the gut and hematopoietic organ. Here we show that the hemocytes (blood cells) are the main regulator of adenosine in the Drosophila larva, as was speculated previously for mammals. The elevated level of adenosine in the hemolymph due to lack of ADGF-A leads to apparently inconsistent phenotypic effects: precocious metamorphic changes including differentiation of macrophage-like cells and fat body disintegration on one hand, and delay of development with block of pupariation on the other. The block of pupariation appears to involve signaling through the adenosine receptor (AdoR), but fat body disintegration, which is promoted by action of the hemocytes, seems to be independent of the AdoR. The existence of such an independent mechanism has also been suggested in mammals.
Atypical Omenn Syndrome due to Adenosine Deaminase Deficiency
Avni Y. Joshi,Erin K. Ham,Neel B. Shah,Xiangyang Dong,Shakila P. Khan,Roshini S. Abraham
Case Reports in Immunology , 2012, DOI: 10.1155/2012/919241
Abstract: We present here a novel case of an atypical Omenn syndrome (OS) phenotype due to mutations in the ADA gene encoding adenosine deaminase. This case is noteworthy for a significant increase in circulating CD56brightCD16- cytokine-producing NK cells after treatment with steroids for skin rash.
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