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The Influence of Temperature, Light and Cytokinins on DNA Content of Musa acuminata (AAA Group) 'Kluai hom thong' and Musa balbisiana (BBB Group) 'Kluai hin'  [cached]
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2008,
Abstract: The changes in DNA content of triploid bananas Musa acuminata (AAA group) 'Kluai Hom Thong' and Musa balbisiana (BBB group) 'Kluai Hin' under different environmental conditions were investigated. The results revealed showed that different temperatures and light regimes had no effects on DNA content of both bananas cultivated on MS (Murashige and Skoog, 1962) medium supplemented with 5 mg/l BA (N6-benzyladenine) and 15% coconut water. However, they had some influences on morphological characteristics. On the other hand, both bananas cultivated on MS medium containing different concentrations of TDZ (N-phenyl-1, 2, 3 thidiazol-5-ylurea; thidiazuron) and BA, differed in their DNA contents. Increase in TDZ and BA concentrations resulted in the decrease of DNA content in 'Kluai Hom Thong', while the DNA content of 'Kluai Hin' increased.
Genetic relationship among subspecies of Musa acuminata Colla and A-genome consisting edible cultivated bananas assayed with ISSR markers
Phruet Racharak,Wichan Eiadthong
Songklanakarin Journal of Science and Technology , 2007,
Abstract: Genetic relationship among subspecies of Musa acuminata and A-genome consisting edible cultivated bananas was investigated by ISSR (inter-simple sequence repeat) markers. Twelve samples of wild type bananas that were classified into 5 subspecies of Musa acuminata, thirty-three samples of edible cultivatedbananas and M. balbisiana were used as plant materials for this study. Of a total of 36 ISSR primers screened, 6 primers revealed a total of 128 alleles, allele size varied from 200 to 3,000 bp with an average of 21.33 alleles per primer, average of allele frequency was 0.18, polymorphic percentage was 1.0 and heterozygositywas 0.29. From the dendrogram, banana samples can be divided into two main clusters with similarity coefficient value at 0.18. The first cluster belonged to the out group which included Musa itinerans and Ensete glaucum, the second cluster belonged to Musa coccinea, M. laterita, all subspecies of M. acuminata, M. balbisiana and all the cultivar groups of the edible cultivated bananas and plantains. In addition, the results indicated two Musa species, consisting of M. coccinea and M. laterita, were sister group of the second cluster as well. All specimens of subspecies of M. acuminata were related to cultivated groups of A-genome consisting of cultivated bananas in Thailand.
Analysis of non-TIR NBS-LRR resistance gene analogs in Musa acuminata Colla: Isolation, RFLP marker development, and physical mapping
Robert NG Miller, David J Bertioli, Franc C Baurens, Candice MR Santos, Paulo C Alves, Natalia F Martins, Roberto C Togawa, Manoel T Souza, Georgios J Pappas
BMC Plant Biology , 2008, DOI: 10.1186/1471-2229-8-15
Abstract: A computational strategy was developed for unbiased conserved motif discovery in NBS and LRR domains in R-genes and homologues in monocotyledonous plant species. Degenerate PCR primers targeting conserved motifs were tested on the wild cultivar Musa acuminata subsp. burmannicoides, var. Calcutta 4, which is resistant to a number of fungal pathogens and nematodes. One hundred and seventy four resistance gene analogs (RGAs) were amplified and assembled into 52 contiguous sequences. Motifs present were typical of the non-TIR NBS-LRR RGA subfamily. A phylogenetic analysis of deduced amino-acid sequences for 33 RGAs with contiguous open reading frames (ORFs), together with RGAs from Arabidopsis thaliana and Oryza sativa, grouped most Musa RGAs within monocotyledon-specific clades. RFLP-RGA markers were developed, with 12 displaying distinct polymorphisms in parentals and F1 progeny of a diploid M. acuminata mapping population. Eighty eight BAC clones were identified in M. acuminata Calcutta 4, M. acuminata Grande Naine, and M. balbisiana Pisang Klutuk Wulung BAC libraries when hybridized to two RGA probes. Multiple copy RGAs were common within BAC clones, potentially representing variation reservoirs for evolution of new R-gene specificities.This is the first large scale analysis of NBS-LRR RGAs in M. acuminata Calcutta 4. Contig sequences were deposited in GenBank and assigned numbers ER935972 – ER936023. RGA sequences and isolated BACs are a valuable resource for R-gene discovery, and in future applications will provide insight into the organization and evolution of NBS-LRR R-genes in the Musa A and B genome. The developed RFLP-RGA markers are applicable for genetic map development and marker assisted selection for defined traits such as pest and disease resistance.Commercial banana varieties, which are mainly derived from Musa acuminata Colla, and M. balbisiana Colla, are cultivated in 130 countries across the tropics and sub-tropics, generating an annual production i
Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4
Robert NG Miller, Marco AN Passos, Natalia NP Menezes, Manoel T Souza, Marcos M do Carmo Costa, Vania C Rennó Azevedo, Edson P Amorim, Georgios J Pappas, Ana Y Ciampi
BMC Research Notes , 2010, DOI: 10.1186/1756-0500-3-148
Abstract: Microsatellite sequences were identified from five Calcutta 4 BAC consensi datasets. Specific primers were designed for 41 loci. Isolated di-nucleotide repeat motifs were the most abundant, followed by tri-nucleotides. From 33 tested loci, 20 displayed polymorphism when screened across 21 diploid M. acuminata accessions, contrasting in resistance to Sigatoka diseases. The number of alleles per SSR locus ranged from two to four, with a total of 56. Six repeat classes were identified, with di-nucleotides the most abundant. Expected heterozygosity values for polymorphic markers ranged from 0.31 to 0.75.This is the first report identifying polymorphic microsatellite markers from M. acuminata subsp. burmannicoides, var. Calcutta 4 across accessions contrasting in resistance to Sigatoka diseases. These BAC-derived polymorphic microsatellite markers are a useful resource for banana, applicable for genetic map development, germplasm characterization, evolutionary studies and marker assisted selection for traits.Commercial banana varieties, which are derived from intraspecific crosses within Musa acuminata Colla, together with interspecific hybrid development with Musa balbisiana Colla, are cultivated mostly by smallholder farmers, across over 120 countries in different tropical and sub-tropical environments. As an inexpensive starch source, banana is also rich in fibre, minerals and vitamins. Although an important food commodity in developing countries in terms of production value after rice, wheat and maize, genetic improvement has been limited. In wild bananas, sexual recombination results in viable seed. However, the majority of today's commercial cultivars are sterile A and B genome-containing triploids, with seedless fruit development occurring via parthenocarpy, partly as a result of translocations [1]. Conventional breeding in Musa diploids and triploids is also hampered as a result of a low number or complete absence of seeds, caused by either a lack of viable polle
Atividade antiviral de Musa acuminata Colla, Musaceae
Martins, Fernanda Otaviano;Fingolo, Catharina Eccard;Kuster, Ricardo Machado;Kaplan, Maria Auxiliadora Coelho;Romanos, Maria Teresa Villela;
Revista Brasileira de Farmacognosia , 2009, DOI: 10.1590/S0102-695X2009000500022
Abstract: this study evaluates the antiviral activity of extracts and fractions of musa acuminata colla collected in two regions of rio de janeiro state (petrópolis and santo ant?nio de pádua). the inflorescences of m. acuminata showed excellent activity for the two virus evaluated: simple human herpesvirus type 1 and simple human herpesvirus type 2, both resistant to acyclovir. the results indicate that the tested extracts of m. acuminata can be potential target for use in antiviral therapy.
Repetitive part of the banana (Musa acuminata) genome investigated by low-depth 454 sequencing
Eva H?ibová, Pavel Neumann, Takashi Matsumoto, Nicolas Roux, Ji?í Macas, Jaroslav Dole?el
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-204
Abstract: In this work, we report on the first thorough characterization of the repeat component of the banana (M. acuminata cv. 'Calcutta 4') genome. Analysis of almost 100 Mb of sequence data (0.15× genome coverage) permitted partial sequence reconstruction and characterization of repetitive DNA, making up about 30% of the genome. The results showed that the banana repeats are predominantly made of various types of Ty1/copia and Ty3/gypsy retroelements representing 16 and 7% of the genome respectively. On the other hand, DNA transposons were found to be rare. In addition to new families of transposable elements, two new satellite repeats were discovered and found useful as cytogenetic markers. To help in banana sequence annotation, a specific Musa repeat database was created, and its utility was demonstrated by analyzing the repeat composition of 62 genomic BAC clones.A low-depth 454 sequencing of banana nuclear genome provided the largest amount of DNA sequence data available until now for Musa and permitted reconstruction of most of the major types of DNA repeats. The information obtained in this study improves the knowledge of the long-range organization of banana chromosomes, and provides sequence resources needed for repeat masking and annotation during the Musa genome sequencing project. It also provides sequence data for isolation of DNA markers to be used in genetic diversity studies and in marker-assisted selection.Bananas and plantains (Musa spp.) are perennial giant herbs grown in humid tropical and subtropical regions. Their annual production exceeds 100 million tons, out of which almost 90% is targeted for local and national markets [1]. Cultivated bananas are parthenocarpic, seed-sterile, vegetatively-propagated diploid, triploid and tetraploid clones. Most of them are hybrids between two diploid (2n = 2x = 22) species M. acuminata and M. balbisiana [2] with the A and B genomes respectively. The production of bananas is threatened by many diseases and pests, b
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 2002,
Abstract: Fourteen populations of Musa acuminata ranging from populations in the lowlands of northern (ssp. siamea) to central Malaysian region (ssp. malaccensis) and highland banana (ssp. truncata) were characterized based on chromosome number and 46 morphological characters. A large amount of variation was observed within the populations. However, only highland bananas appeared morphologically distinct. Lowland populations both from northern and central Malaysia were found to be overlapping and no distinguishing pattern was observed. The morphological characters found variable within these populations were related to developmental changes and mutations. The results ob tained in this study were not revolutionary. However, the survey of a large number of characters treated with multivariate techniques further sharpened the existing groupings of the Musa acuminata subspecies.
Valorization and Miscellaneous Prospects of Waste Musa balbisiana Colla Pseudostem  [PDF]
Krishna Gogoi,Mayur Mausoom Phukan,Nipu Dutta,Salam Pradeep Singh,Pitambar Sedai,Bolin Kumar Konwar,Tarun Kumar Maji
Journal of Waste Management , 2014, DOI: 10.1155/2014/412156
Abstract: Resourceful utilization of the enormous quantum of agrowastes generated via agricultural practices can be supportive in waste management, environmental upgradation, and subsequent material and energy recovery. In this regard, the present study aimed at highlighting waste banana (Musa balbisiana Colla) pseudostem (an agrowaste) as a potential bio-based feedstock with miscellaneous applications. The pseudostem was characterized by carbon, nitrogen, and hydrogen (CHN) analysis, thermogravimetric-differential thermal analysis (TGDTA), and Fourier transform infrared (FTIR) spectroscopy. Cellulose, hemicellulose, and lignin were estimated as a part of biochemical characterization. Total phenolic content, total flavonoid content, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, and ferric reducing antioxidant power (FRAP) were carried out as a part of antioxidant characterization. The waste banana pseudostem biomass (WBPB) was also tried successfully as a natural filler in polyvinyl chloride (PVC) polymer composite. Thermal properties and water uptake test of the WBPB polymer composite were accessed as a part of composite characterization. The pseudostem had calorific value (15.22?MJ/kg), high holocellulose (58.67%), high free radical scavenging potential (69.9%), and a low ash content (6.8%). Additionally, the WBPB polymer composite showed improved water resistance and thermostability. The study suggests feasibility of WBPB as a prospective bioenergy feedstock, primary antioxidant source, and reinforcing agent in polymer composites. 1. Introduction With green revolution hitting the block and subsequent intensification in agricultural practices, speedy increase in volumes and types of agrowastes generated is indispensable. Wide scale global distribution, renewable nature, and zero cost accessibility have already highlighted the importance of agrowastes as a potential resource for material and energy recovery. On a global scale, 140 billion metric tons of biomass are generated every year [1] of which a considerable proportion is agrowaste. Judicious utilization of this enormous quantum of agrowastes (lignocellulosic biomass) for material and energy recovery would undoubtedly aid in solving the menace of waste management and resource depletion. Lignocellulosic biomass which represents a renewable and largely untapped source of raw feedstock for conversion into liquid and gaseous fuels, thermochemical products, and other energy-related end products [2] can assist in anthropogenic endeavors for combating energy crisis and promoting sustainable
The Influence of Explant Types and Orientation on in Vitro Culture of Musa balbisiana ‘Kluai Hin’ (BBB group)
Notulae Scientia Biologicae , 2011,
Abstract: Inflorescence, apical and lateral buds of Musa balbisiana ‘Kluai Hin’ (BBB group) were used to culture on MS medium supplemented with 22 μM BA and 15% (v/v) coconut water. Comparison of bud orientation showed that the best response of in vitro shoot tip proliferation was obtained with abaxial surface of buds lying down i.e. one side touching the medium (tilt). Mass propagation of shoot tips was obtained when cultured buds on MS medium containing 44 μM BA. Rooting was achieved by transferring the isolated shoots to MS basal medium without growth regulators. Rooted plantlets were acclimatized and successfully established in soil.
Polyploid Induction in a Local Wild Banana (Musa acuminata ssp. malaccensis)  [PDF]
Asif M. J.,C. Mak,O. Rofina Yasmin
Pakistan Journal of Biological Sciences , 2000,
Abstract: Flow cytometry was successfully used for screening ploidy levels in a large population of in vitro induced autopolyploids of the Musa acuminata ssp. malaccensis a wild banana. Use of zygotic embryo culture for large production of banana seed progenies and improved media compositions has facilitated the process of polyploidy induction. Treating shoot apices with 0.5% colchicine in combination with 2% DMSO for 2 h successfully produced tetraploids in Musa. Tetraploids produced were further confirmed through chromosomal counts.
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