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Antimicrobial activity of some Iranian medicinal plants  [PDF]
Ghasemi Pirbalouti Abdollah,Jahanbazi Parvin,Enteshari Shekoofeh,Malekpoor Fatemeh
Archives of Biological Sciences , 2010, DOI: 10.2298/abs1003633g
Abstract: The major aim of this study was to determine the antimicrobial activity of the extracts of eight plant species which are endemic in Iran. The antimicrobial activities of the extracts of eight Iranian traditional plants, including Hypericum scabrum, Myrtus communis, Pistachia atlantica, Arnebia euchroma, Salvia hydrangea, Satureja bachtiarica, Thymus daenensis and Kelussia odoratissima, were investigated against Escherichia coli O157:H7, Bacillus cereus, Listeria monocytogenes and Candida albicans by agar disc diffusion and serial dilution assays. Most of the extracts showed a relatively high antimicrobial activity against all the tested bacteria and fungi. Of the plants studied, the most active extracts were those obtained from the essential oils of M. communis and T. daenensis. The MIC values for active extract and essential oil ranged between 0.039 and 10 mg/ml. It can be said that the extract and essential oil of some medicinal plants could be used as natural antimicrobial agents in food preservation. .
Antimicrobial Activity of five medicinal plants of Bangladesh  [PDF]
Sharmin Reza Chowdhury,Shahana Akter,Tasnuva Sharmin,Farhana Islam
Journal of Pharmacognosy and Phytochemistry , 2013,
Abstract: The antimicrobial activity of five medicinal plants was evaluated by disc diffusion method. Among those, some fraction of plant extracts (400 μg /disc) exhibited potent antimicrobial activity against five gram positive and seven gram negative bacteria and three fungi. Among the test samples of O. mungos, the carbon tetrachloride soluble fraction exhibited 12.0 mm and 13.0 mm zone of inhibition against B. megaterium and Aspergillus niger respectively. The crude methanol extract of S. nodiflora exhibited 14.0 mm zone of inhibition against Bacillus cereus whereas the carbon tetrachloride soluble fraction revealed 16.0 mm against Shigella boydii. The chloroform soluble fraction of P. sagitatta exhibited 16.0 mm zone of inhibition against Staphylococcus aureus and Shigella boydii. Among the test samples of M. macrophylla, the carbon tetrachloride soluble fraction showed 15.0 mm zone of inhibition against Salmonella paratyphi and 13.0 mm zone of inhibition against Aspergillus niger. The crude methanol extract of G. philippensis exhibited 18.0 mm zone of inhibition against Bacillus cereus.
Antimicrobial Activity of Some Turkish Medicinal Plants  [PDF]
B. Dulger,A. Gonuz
Pakistan Journal of Biological Sciences , 2004,
Abstract: In this study, antimicrobial activity of Rhododendron ponticum L., Prunus laurocerasus L., Agrimonia eupatoria L., Cornus mas L., Vitis vinifera L., Punica granatum L., Anthemis cotula L., Cichorium intybus L., Viscum album L., Papaver hybridum L., Malva rotundifolia L. and Rhus coriaria L. were investigated. The ethanolic extracts of these plants were tested against Escherichia coli ATCC 11230, Staphylococcus aureus ATCC 6538P, Klebsiella pneumoniae UC57, Pseudomonas aeruginosa ATCC 27853, Proteus vulgaris ATCC 8427, Bacillus cereus ATCC 7064, Mycobacterium smegmatis CCM 2067, Listeria monocytogenes ATCC 15313, Micrococcus luteus CCM 169, Candida albicans ATCC 10231, Rhodotorula rubra DSM 70403 and Kluyveromyces fragilis ATCC 8608 by disc diffusion method. Of the 12 plants tested, nine showed antimicrobial activity. Each plant species has unique against different microorganisms. The fruit extract of Rhus coriaria had the highest antimicrobial effect with an inhibition zone of 12-52 mm against all the bacteria, but not shown antiyeast effect. Except for the extracts of Rhus coriaria, Agrimonia eupatoria and Anthemis cotula, all additional extracts of generated inhibition zones smaller than those generated by several reference antibiotics.
ANTIMICROBIAL ACTIVITY OF FEW SELECTED MEDICINAL PLANTS
Dash G. K,Murthy P. N.
International Research Journal of Pharmacy , 2011,
Abstract: The petroleum ether, chloroform, methanol and aqueous extracts of leaves of Ageratum conyzoides Linn (Fam: Asteraceae), Argemone mexicana Linn. (Fam: Papaveraceae), Heliotropium indicum Linn (Fam: Boraginaceae) and stem barks of Alstonia scholaris (L.) R. Brown (Fam: Apocynaceae) were screened for their antimicrobial activity against Bacillus subtilis, Stapphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger respectively. The results indicated that the chloroform, methanol and aqueous extracts of all tested plant materials are active against both Gram-positive and Gram-negative bacteria at the tested concentration. The spectrum of activity observed in the present study may be an indicative of the presence of broad spectrum antimicroial compounds in the extracts. Among the tested extracts, methanol extracts of all selected plant materials were found to be more effective than the other extracts under study. Preliminary phytochmical screening of the methanol extracts of selected plant materials primarily revealed presence of alkaloids, tannins and flavonoids. The present work justifies the use of these plant materials for antimicrobial activity as claimed in the folklore remedies.
Antimicrobial activity of selected South African medicinal plants
Trine RH Nielsen, Victor Kuete, Anna K. J?ger, Johannes J Marion Meyer, Namrita Lall
BMC Complementary and Alternative Medicine , 2012, DOI: 10.1186/1472-6882-12-74
Abstract: The radiometric respiratory technique using the BACTEC 460 system was used for susceptibility testing against Mycobacterium tuberculosis, and the liquid micro-broth dilution was used for other antimicrobial assays.The results of the minimal inhibitory concentration (MIC) determinations indicated that the methanol extracts from Acacia karoo, Erythrophleum lasianthum and Salvia africana were able to prevent the growth of all the tested microorganisms. All other samples showed selective activities. MIC values below 100?μg/ml were recorded with A. karoo, C. dentate, E. lasianthum, P. obligun and S. africana on at least one of the nine tested microorganisms. The best activity (MIC value of 39.06?μg/ml) was noted with S. africana against E. coli, S. aureus and M. audouinii, and Knowltonia vesitoria against M. tuberculosis.The overall results of the present work provide baseline information for the possible use of the studied South African plant extracts in the treatment of microbial infections.
Antimicrobial activity of some ethno-medicinal plants used in Pakistan
Sajid Bashir,Alia Erum,Rizwana Kausar,UzmaSaleem
Research in Pharmacy , 2012,
Abstract: Medicinal plants have been used for their antimicrobial activity to discover newantimicrobial agents. Antimicrobial activity of aqueous and methanolic extracts of fourethnomedicinal plants such as Acacia modesta, Thymus serphylum, Syzygium cumuni L andOlea ferruginea were evaluated against five bacterial strains such as S. aureus, E. coli, P.aeruginosa, S. epidermidis and B. subtilis by disc diffusion method. The results showed thatAcacia modesta showed maximum activity against E. coli with zone of inhibition (16.2 mm).Thymus serphylum showed maximum activity against B. subtilis with zone of inhibition(13.3 mm) but no activity against P. aeruginosa and E. coli. Syzygium cumuni L showedmaximum activity against S.epidermidis with zone of inhibition (14.5 mm) but no activityagainst E. coli, P. aeruginosa and B. subtilis. Similarly Olea ferruginea displayed maximumactivity against S. epidermidis with zone of inhibition (16.8 mm). Antimicrobial activity ofplants was concentration dependent. Methanolic extracts of plants showed better activitythan aqueous extracts.
ANTIMICROBIAL ACTIVITY OF SOME MEDICINAL PLANTS AGAINST FISH PATHOGENS  [PDF]
Sharma Madhuri,Mandloi A.K.,Pandey Govind,Sahni Y.P.
International Research Journal of Pharmacy , 2012,
Abstract: The present article elucidates on the antimicrobial (antibacterial and antifungal) activity of some medicinal plants (herbs) against different microbes (e.g., bacteria and fungi). Aquaculture has been a growing activity for more than 20 years worldwide. The bacterial infections are considered the major cause of mortality in aquaculture. Among the common fish pathogenic bacteria, Streptococcus agalactiae, Lactococcus garvieae, Enterococcus faecalis (all gram-positive), Aeromonas hydrophila and Yersinia ruckeri (both gram-negative) cause infectious diseases. A. hydrophila, the most common bacterial pathogen in freshwater fish, has been recognized to be the aetiological agent of many pathological conditions, including tail rot, motile Aeromonas septicemia and epizootic ulcerative syndrome as a primary pathogen. The continuous use of antimicrobial agents in aquaculture has resulted into resistant bacterial strains in the aquatic environment. Treatment of bacterial diseases with different herbs has been safely used in organic agriculture, veterinary and human medicine. Treatment with medicinal plants having antibacterial activity is a potentially beneficial alternative in the aquaculture. These herbs mitigate many of the side effects which are associated with synthetic antimicrobials. Additionally, the plant-derived phytomedicines provide a cheaper source for treatment and greater accuracy than chemotherapeutic agents. Plants have been used as traditional medicine since time immemorial to control bacterial, viral and fungal diseases. In India, 500 medicinal plant species are used against pathogenic bacteria. Recently, research has been initiated to evaluate the feasibility of herbal drugs in fish diseases. Because of the growing bacterial resistance against commercial standard and reserve antibiotics, the search for new active substances with antibacterial activity against pathogenic bacteria is of increasing importance.
Antimicrobial Activity of Some Medicinal Plants Against Candida albicans  [PDF]
Dhia Hassawi,Abeer Kharma
Journal of Biological Sciences , 2006,
Abstract: Four extract amounts (200, 150, 100 and 50 mg mL-1) from twelve medicinal plant species that belong to six genera (Achillea, Salvia, Convolvulus, Plantago, Anthemis and Artemisia) were tested against Candida albicans. The antimicrobial activity was carried out by using the hole-plate diffusion method. The effect of plant species, extract amounts and their interaction were highly significant. Achillea santolina, Salvia dominica and Salvia officinalis inhibited the growth of Candida albicans at all tested extract amounts. The extracts of (Salvia spinosa, Convolvulus althaeoides and Plantago lanceolata) showed no activity against Candida albicans.
Assessment Of Biological Activity Of Brazilian Medicinal Plants Against Artemia Salina Leach
Rinalda Araújo Guerra de Oliveira, Marcus Ant?nio de Brito Folly , Aline Lira Xavier, Margareth de Fátima Formiga Melo Diniz, Edeltrudes de Oliveira Lima
Revista de Enfermagem UFPE On Line , 2010,
Abstract: Objective: to evaluate the biological activity of extracts of Pithecellobium cochliocarpum, Momordica charantia and Ipomoea asarifolia isolated and associated with the Brine Shrimp test. Method: solutions of different concentrations of the extracts was prepared and metanauplius of Artemia salina were added. Each concentration was tested in triplicate, repeated in two experiments. The set was left in incubation under artificial light for 24 hours and after this time the counting of the number of live larvae and dead was done. It was determined the LC50 in accordance with the statistical method of Probits. Results: the LC50 value obtained for the extracts of Pithecellobium cochliocarpum, Momordica charantia and Ipomoea asarifolia when tested alone was: 543.5 ppm, 400.9 ppm and 916.3 ppm, respectively. For the association of Pithecellobium cochliocarpum and Ipomoea asarifolia, Pithecellobium cochliocarpum and Momordica charantia, and Momordica charantia and Ipomoea asarifolia, the LC50 found was respectively: 641.0 ppm, 502.2 ppm and 706.4 ppm. The association of the three extracts showed an LC50 of 990.8 ppm. Conclusion: all extracts showed biological activity against Artemia salina and their associations can lead to interactions among its constituents. More detailed pharmacological studies should be conducted. Descriptors: Artemia; Plants medicinal; toxicity; associations.
Evaluation of the Antimicrobial Activity of Three Medicinal Plants of South India
Jamuna, B. A.,Ravishankar, R. V.,Pradeepa, V. S.
Malaysian Journal of Microbiology , 2011,
Abstract: The present study was carried out to evaluate the antimicrobial activity of the crude methanolic extracts of Memecylon malabaricum Clarke. (leaves), Cochlospermum religiosum Linn. (leaves and flowers) and Andrographis serpyllifolia Vahl. (leaves) using the standard disc diffusion assay against eight strains of bacterial species, viz., Staphylococcus aureus, Salmonella typhi, Enterobacter aerogenes, Pseudomonas aeruginosa, Xanthomonas oryzae pv. oryzae, Xanthomonas axonopodis pv. malvacearum, Bacillus cereus and Micrococcus sp. The extracts of the plants at a concentration of 1.25 mg/disc showed minimum to moderate activity against both Gram positive and Gram negative bacteria indicating a broad spectrum activity. A preliminary phytochemical screening was conducted on the selected plant extracts using standard qualitative procedures that revealed the presence of several secondary metabolites. The extracts failed to show antioxidant activity by reducing power assay. The result indicates the potential usefulness of these plants especially Memecylon malabaricum and Cochlospermum religiosum, in treating microbial infections in humans and plants and justifies the need for further investigations and characterization of the bioactive compounds present in the methanolic extracts of the plants.
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