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Methodo para diagnostico do alastrim
Torres, C. Magarinos;Teixeira, J. de Castro;
Memórias do Instituto Oswaldo Cruz , 1937, DOI: 10.1590/S0074-02761937000400007
Abstract: inclusion bodies of alastrim are quite consistent in their morphology and staining properties when studied in material from seven epidemies occurring in several states of brazil (pará, minas geraes, rio de janeiro, districto federal and s?o paulo) from 1932 to 1937. paranuclear or circumnuclear basophilie cytoplasmic bodies not stained by safranine, single or in pairs at opposite ends of the nuclei could always be demonstrated in epidermal cells from skin lesions either in man or in macaca mulatta. cytoplasmic inclusion bodies of variola vera as seen in human cases, and of vaccinia as seen in macaca mulatta are acidophilic or polychromatophilic and deeply stained by safranine. a method for the diagnosis of alastrim is devised taking into account the sensibility of macaca mulatta to the virus, and the morphology and staining properties of the cytoplasmic inclusion bodies as seen in skin lesions of the monkey. this method has been successfully tried in epidemies occurring at the states of pará (1936), s?o paulo (1936) and districto federal (1937) when the real diagnosis was a matter of discussion.
Estudo comparativo das inclus?es do alastrim e da vacina no macaco (Macacus rhesus)
Torres, C. Magarinos;Teixeira, J. de Castro;
Memórias do Instituto Oswaldo Cruz , 1934, DOI: 10.1590/S0074-02761934000100006
Abstract: 1. - vesicles and pustules containing numerous cytoplasmic inclusion bodies within the epidermal cells were regularly produced in monkeys (macacus rhesus) by intravenous inoculation either of alastrim virus or of recently prepared vaccine emulsion, no previous scarifications being required. alastrim virus seems less virulent for this species of monkey than the virus of vaccinia is. while 12 rhesus monkeys injected intravenously with seven strains of alastrim virus developed regularly an experimental infection and finally recovered and remained well, the intravenous injection of vaccine virus was followed by death in two from four rhesus monkeys injected. 2. slight but definite and regular differences were noticed between alastrim and vaccine bodies after fixation in helly's fluid, embedding in paraffin and staining with alum hematoxylin and eosin. they are related to the number of inclusions within the cell and to their staining. 3. - in each cell of the epidermis of macacus rhesus there is usually a single alastrim body when late stages are considered, and quite a few (2-4) when early stages are observed. alastrim bodies stain a deep blue to grayish-blue. nevertheless, in necrotic epidermal cells or in cells provided with a few small inclusions they sometimes present a pink staining. 4. - vaccine bodies in late stages of development are numerous in each epidermal cell of the rhesus monkey and present regularly a characteristic polychromatophilia.
Estudo comparativo das inclus?es do alastrim e da variola vera
Torres, C. Magarinos;Teixeira, J. de Castro;
Memórias do Instituto Oswaldo Cruz , 1935, DOI: 10.1590/S0074-02761935000800003
Abstract: resulta das descrip??es e documentos examinados que as inclus?es cytoplasmaticas da variola vera, no material humano examinado, apresentam caracteres geraes que poder?o ser assim resumidos: 1. intensa colora??o pela safranina nos preparados pelo methodo de unna modificado (fig. 42), mostrando a inclus?o matiz semelhante ao dos nucleolos da mesma cellula. 2. reac??o predominantemente acidophila nos preparados pela hematoxylina-eosina, traduzida pela tonalidade rosea ou vermelha de colora??o (figs. 27, 29, 30, 31 e 31). 3. frequente multiplicidade de inclus?es de fórma e dimens?es variadas na mesma cellula (figs. 29 e 30), as maiores inclus?es (figs. 29 e 32), sendo menores que as grandes inclus?es cytoplasmaticas solitarias do alastrim (figs. 15, 16 e 18). é a regra, ainda, observar-se desapparecimento de qualquer estructura no cytoplasma das cellulas com inclus?es em zona muito extensa, tornando-se difficil explicar tal aspecto unicamente pela retrac??o das inclus?es no acto da fixa??o (figs. 31 e 32). quanto ás inclus?es intranucleares, ellas se apresentam sob tres aspectos, dois dos quaes bem reconhecidos por luger e lauda (1926). em um primeiro aspecto, o nucleo conserva, em parte, o reticulo de linina e encerra uma massa irregular (fig. 35) ou, ent?o, pequenas massas e granulos de dimens?es variaveis (figs. 33 e 34) constituidos por material acidophilo que ali n?o existe em condi??es normaes. a membrana nuclear tem espessura visinha do normal. em um segundo aspecto, a inclus?o occupa a totalidade do nucleoplasma (fig. 3), apenas separado da membrana nuclear, em alguns casos (fig. 38), por um estreito espa?o claro (zona de retrac??o). por vezes apresenta um aspecto homogeneo (fig. 37); outras vezes a inclus?o mostra pequenas areas chromophobas ( fig. 38). a hyperchromatose da membrana nuclear é accentuada, tanto neste como no aspecto seguinte. no terceiro aspecto (aspecto corpuscular) (figs. 39, 40, 48 e 50), a inclus?o intranuiclear é formada por um ou mais corp
Altera??es microscópicas na erup??o do Sarampo
Torres, C. Magarinos;
Memórias do Instituto Oswaldo Cruz , 1952, DOI: 10.1590/S0074-02761952000100001
Abstract: hyaline necrosis of epidermal cells either single or in clumps represents apparently the primary change in the measles eruption. lesions occur, however, very soon in the corium and could be demonstrated twelve hours after the onset of the eruption. the early lesions (twelve to thirty-six hours) in the epidermis show usually different stages in a single slide examined. they are described as minute vesicles and pustules; in older lesions the pustules have dried up forming thickened plaques in and beneath cornified layer. parakeratotic cells with intranuclear bodies first described by torres & teixiera (1932 b) while inconstant are regarded as a pathognomonic change in measles eruption. edema of the papillary layer and perivascular infiltrations in the reticular layer by large mononuclears some of them containing small irregular deeply stained granules (mallory-medlar-lipschütz' cells) are well known changes largely referred in the literature. evidence is here submitted in support of the opinion that such cells correspond to macrophages with keratohyaline granules phagocited as a consequence of changes in cornification determined by the virus itself. microscopic examination is necessary for the demonstration of the minute vesicles and pustules which are such an important detail in the histology of the measles eruption as it establishes connections between measles usually considered in the group of exanthematous diseases with chicken-pox, zoster, small-pox and alastrim (pustulous diseases). epidermal changes are no more found seventy-two hours after the onset of the eruption while well-defined mantles of cells about the vessels and a moderate proliferation of fibrocytes is noticed in the corium.
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