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Radiographic studies of developing calvaria at prenatal stages in one-humped camel
SA Hena
Sokoto Journal of Veterinary Sciences , 2012,
Abstract: Radiographic studies on the fetal heads of 32 one-humped camels (Camelus dromedarius) with 11 fetuses at the first trimester, 12 at the second trimester and 9 at the third trimester levels were conducted in Sokoto Metropolis. The study involved the radiographic evaluation of calvaria of different fetuses at first, second and third trimester stages of the gestational ages. Radiographic observations made in this study revealed that the onset of mineralization was recognizable even at the first trimester stage through the radio-opaque appearances of the calvaria appearing whiter on radiographs. This tendency however increased with increase in gestational age of the fetuses showing that because of their highly calcified calvaria, the fetuses at the third trimester were more radioopaque to X-ray energy thus appearing whiter on the radiographs. Sutural demarcations were not clear on the radiographs made in this study, but however, clear evidences of fontanels were seen as localised radio-lucent areas on the calvaria more especially at the first trimester stage. The findings in this research would help in understanding the radiographical anatomy of the developing calvaria in this animal species.
Radiographic studies of developing calvaria at prenatal stages in one-humped camel
SA Hena,ML Sonfada,BI Onyeanusi,ROC Kene
Sokoto Journal of Veterinary Sciences , 2012,
Abstract: Radiographic studies on the fetal heads of 32 one-humped camels (Camelus dromedarius) with 11 fetuses at the first trimester, 12 at the second trimester and 9 at the third trimester levels were conducted in Sokoto Metropolis. The study involved the radiographic evaluation of calvaria of different fetuses at first, second and third trimester stages of the gestational ages. Radiographic observations made in this study revealed that the onset of mineralization was recognizable even at the first trimester stage through the radio-opaque appearances of the calvaria appearing whiter on radiographs. This tendency however increased with increase in gestational age of the fetuses showing that because of their highly calcified calvaria, the fetuses at the third trimester were more radio-opaque to X-ray energy thus appearing whiter on the radiographs. Sutural demarcations were not clear on the radiographs made in this study, but however, clear evidences of fontanels were seen as localised radio-lucent areas on the calvaria more especially at the first trimester stage. The findings in this research would help in understanding the radiographical anatomy of the developing calvaria in this animal species.
Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
Spin-Neto, Rubens;Coletti, Felipe Leite;Freitas, Rubens Moreno de;Pavone, Chaíne;Campana-Filho, Sérgio Paulo;Marcantonio, Rosemary Adriana Chiérici;
Revista de Odontologia da UNESP , 2012, DOI: 10.1590/S1807-25772012000500003
Abstract: objective: this study evaluated, using digital radiographic images, the action of chitosan and chitosan hydrochloride biomaterials, with both low and high molecular weight, used in the correction of critical-size bone defects (csbd's) in rat's calvaria. material and method: csbd's with 8 mm in diameter were surgically created in the calvaria of 50 holtzman rats and these were filled with a blood clot (control), low molecular weight chitosan, high molecular weight chitosan, low molecular weight chitosan hydrochloride and high molecular weight chitosan hydrochloride, for a total of 10 animals, which were divided into two experimental periods (15 and 60 days), for each biomaterial. the radiographic evaluation was made using two digital radiographs of the animal's skull: one taken right after the bone defect was created and the other at the moment of the sacrifice, providing the initial and the final radiographic bone density in the area of the defect, which were compared. result: analysis of radiographic bone density indicated that the increase in the radiographic bone density of the csbd's treated with the proposed biomaterials, in either molecular weight, in both observed periods, where similar to those found in control group. conclusion: tested chitosan-based biomaterials were not able to enhance the radiographic density in the csbd's made in rat's calvaria.
Radiographic and Histological Findings in an Atypical Orbital Myxoma
BP. Bielory, NM. Mirani, MT. Barry and RE. Turbin
Ophthalmology and Eye Diseases , 2012, DOI: 10.4137/OED.S7498
Abstract: Myxomas are rare neoplasms of mesenchymal origin. Diagnostic evaluation requires histopathological confirmation. We report a rare case of atypical orbital myxoma with radiographic and histopathological correlation.
Clinical, radiographic and histological evaluation of chronic periapical inflammatory lesions
Lia, Raphael Carlos Comelli;Garcia, Juliana Máira Q.;Sousa-Neto, Manoel D.;Saquy, Paulo César;Marins, Renata Hebling;Zucollotto, Wagner Grandinni;
Journal of Applied Oral Science , 2004, DOI: 10.1590/S1678-77572004000200007
Abstract: the aim of the present study was to comparatively evaluate the clinical, radiographic and histological aspects of chronic inflammatory periapical lesions. one hundred and sixty-four lesions of human teeth, independent of age, race and sex, were evaluated by clinical, radiographic and histological analyses conducted after surgical endodontic treatment. our results showed that there was uniformity in the interference factors on the evolution of periapical healing such as the apical biofilm and/or endogenous (cholesterol crystals) or exogenous (extruded endodontic materials that are indigestible or of difficult digestion) foreign bodies and that some cysts are reversible with endodontic treatment. we conclude that it is difficult for the clinician to adjust parameters defining a diagnosis of chronic inflammatory periapical lesions.
Organic Bovine Graft Associated With PRP In Rabbit Calvaria  [cached]
Rocha, Flaviana Soares,Ramos, Lara Maria Alencar,Batista, Jonas Dantas,Zanetta-Barbosa, Darceny
International Archives of Otorhinolaryngology , 2011,
Abstract: Introduction: Repairing large bone defects is a huge challenge that reconstructive surgery currently faces. Objective: The objective of this study was to perform the histological evaluation of bone repair in rabbit calvaria when using bovine bone graft (Gen-ox-organic ) associated with platelet-rich plasma (PRP). Method: 12 rabbits were used and two bone fragments were bilaterally removed from calvaria. Then, 24 surgical sites were randomly divided into 3 groups: coagulum (group I), organic (group II) and PRP-included organic (group III). After four weeks, the animals were sacrificed and the grafted area removed, fixed in 10% formalin with PBS 0.1 M, and embedded in paraffin. Study method:The analyzed histological parameters were: defective area filled with the newly-formed bone, graft's giant cells and particles, as well as the new bone formation associated with the particles. Group I's defects were filled with fibrous tissue attaching the periosteum and revealed a little bone formation peripherally. In both groups II and III, a similar standard was noticed in addition to the absence of graft particles and giant cells. There was no significant difference in the number of giant cells, graft particles and new bone formation around the particles between the grafted material and the PRP-related group. Conclusion: The results achieved indicate that the organic biomaterial neither separately nor jointly with PRP improves bone regeneration.
Radiographic and histological evaluation of bisphosphonate alendronate and metotrexate effects on rat mandibles inoculated with Walker 256 carcinosarcoma
Alves, Ana Paula Negreiros Nunes;Pessoa, Cláudia do ó;Costa-Lotufo, Letícia;Moraes Filho, Manoel Odorico;
Acta Cirurgica Brasileira , 2007, DOI: 10.1590/S0102-86502007000600008
Abstract: purpose: to investigate the effects of bisphosphosnate alendronate (ald) and metotrexate (mtx) on an experimental model of walker 256 carcinosarcoma developed in the oral cavity of rats. methods walker 256 carcinosarcoma cell suspension (0,1 ml) containing 106 cell/ml was implanted in the alveoli of the first and second molars. the animals were divided and treated with saline, mtx, ald, and mtx plus ald. later, the animals were sacrificed, the tumors were measured and the mandibles were removed for radiographic and histological analysis. results: in the control group, the radiographic images demonstrated radioluscency with poorly defined borders, and the microscopic examination revealed tumor infiltration into the peripheral and central regions of the bone. areas of necrosis were commonly seen. in the treated groups with ald, associated or not with mtx, the radiographic analysis revealed circumscribed tumor-induced osteolysis and various degrees of radiotransparence; while, histologically, preserved bone trabeculae with osteoid formation was observed among malignant cells. conclusion: the bisphosphonate alendronate exherted an osteoprotective effect and induced bone neoformation on the walker 256 carcinosarcoma inoculated in rat mandibles. the combination of metotrexate with bisphosphonate alendronate is more successful than treatment with the agents alone in controlling the growth of neoplastic cells and in stimulating reactive new bone. therefore, this may be an alternative treatment to malignant lesions of maxillaries with osteolysis.
Comparison of the human bone matrix gelatin (HBMG) with autogenous bone graft in reconstruction of the parietal bone defects in rat: a histological and radiographic study  [cached]
Hossein Shahoon,Hamid Reza Azimi,Camellia Kianbakht
Journal of Dental Research, Dental Clinics, Dental Prospects , 2009,
Abstract: Background and aims. Autogenous bone graft is commonly used for reconstruction of bone defects in routine surgical procedures. The complexity of producing bone grafts and their application has lead to the use of human bone matrix gelatin (HBMG). The present study was conducted to compare the efficacy of HBMG and autograft on the reconstruction of bone defects in rats. Materials and methods. In this cross-sectional, experimental study, two defects were put on left and right sides of parietal bone of rats. HBMG was placed randomly on defects of one side and autograft in the defects of the other side. All specimens were assessed and compared with each other according to histological and radiographic characteristics. Other assessments included amount and the rate of bone formation, inflammation signs, fibrosis tissue and cartilage formation and also radiographic characteristics of grafts, assessed by digital and film-based methods. Mann-Whitney U test was used for statistical analysis. Results. The results showed a reduction of inflammation and an increase in new bone formation in both groups in 7, 14, 28 and 60 days after surgery. Bone formation with HBMG on day 24 was more than autograft. However, there was no significant difference between the groups on day 60. Superiority of digital method to film-based method of imaging was also observed. Conclusion. Although HBMG has the same efficacy as autograft, the rate of bone reconstruction with HBMG is higher. HBMG also induces focal, rather than peripheral, bone construction in the defect.
Clinical, radiographic, biochemical and histological findings of florid cemento-osseous dysplasia and report of a case
Gon?alves, Marcelo;Píspico, Ronaldo;Alves, Fábio de Abreu;Lug?o, Carlos Eduardo B.;Gon?alves, Andréa;
Brazilian Dental Journal , 2005, DOI: 10.1590/S0103-64402005000300014
Abstract: florid cemento-osseous dysplasia has been described as a condition that characteristically affects the jaws of middle-aged black women. it usually manifests as multiple radiopaque cementum-like masses distributed throughout the jaws. this condition has also been classified as gigantiform cementoma, chronic sclerosing osteomyelitis, sclerosing osteitis, multiple estenosis and sclerotic cemental masses. the authors present a case of an uncomplicated florid cemento-osseous dysplasia in a 48-year-old black woman. multiple sclerotic masses with radiolucent border in the mandible were identified radiographically. histopathologic findings revealed formation of calcified dense sclerotic masses similar to cementum. all clinical, radiographic, biochemical and histological features were suggestive of the diagnosis of florid cemento-osseous dysplasia.
Histomorphometric analysis of the repair process of autogenous bone grafts fixed at rat calvaria with cyanoacrylate
Esteves, J?natas Caldeira;Borrasca, Albanir Gabriel;Aranega, Alessandra Marcondes;Garcia Junior, Idelmo Rangel;Magro Filho, Osvaldo;
Journal of Applied Oral Science , 2011, DOI: 10.1590/S1678-77572011000500016
Abstract: objective: the purpose of this study was to perform histological and histometric analyses of the repair process of autogenous bone grafts fixed at rat calvaria with ethyl-cyanoacrylate adhesive. material and methods: thirty-two rats were divided into two groups (n=16), group i - control and group ii - adhesive. osteotomies were made at the right parietal bone for graft obtainment using a 4-mm-diameter trephine drill. then, the bone segments were fixed with the adhesive in the parietal region of the opposite side to the donor site. after 10 and 30 days, 8 animals of each group were euthanized and the calvarias were laboratorially processed for obtaining hematoxylin and eosin-stained slides for histological and histometric analyses. results: an intense inflammatory reaction was observed at the 10-day period. at 30 days, this reaction was less intense, despite the presence of adhesive at the recipient-site/graft interface. graft incorporation to the recipient site was observed only at the control group, which maintained the highest graft size at 10 and 30 days. conclusions: although the fragment was stable, the presence of adhesive in group ii did not allow graft incorporation to the recipient site, determining a localized, discrete and persistent inflammatory reaction.
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