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The role of polymorphism at αs1-casein locus on milk fatty acid composition in Girgentana goat  [cached]
Bernardo Valenti,Renato Italo Pagano,Pietro Pennisi,Marcella Avondo
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2009.s2.441
Abstract: Sixteen lactating Girgentana goats were used to evaluate the effect of polymorphism at αs1-casein locus on milk fatty acids composition. Animals, homogeneous for milk production, days of lactation and body weight, were divided into two groups: eight homozygous for strong allele (AA group) and eight homozygous for weak allele (FF group). The experimental diet, identical for the two groups, consisted of alfalfa hay (1.5 kg), whole barley, whole maize, pelleted sunflower and whole faba bean (0.5 kg each). In spite of identical selected diets, also in terms of fatty acids, milk fatty acid composition resulted different between the two groups. In particular, except for C8:0, short and medium chain fatty acids and odd chain fatty acids resulted in higher percentage in the AA group. Taking in account that the difference reported in our experiment concerns above all de novo synthesized fatty acids, our results seem to confirm the hypothesis that polymorphism at αs1-casein locus can influence milk fatty acid composition in goats.
Genetic polymorphism of the K-casein (CSN3) gene in goats reared in Southern Italy  [cached]
Silvia Reale,Mohamed Habib Yahyaoui,Josè Maria Folch,Armand Sanchez
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2005.97
Abstract: K-casein (K-CN) represents one of the most important proteins determining the manufacturing properties of milk, because of its essential role in micelle formation and stabilisation. Several genetic variants of K-CN have been described in goats. To investigate the occurrence of seven alleles and their distribution among breeds, a total of 170 animals, from six different breeds reared in Italy (Cilentana Nera, Derivata di Siria, Maltese, Jonica, Garganica and Cashmere), have been analysed in this paper by the primer extension method. Alleles A and B were found to be the most represented in all the analysed breeds; allele D is present only in Maltese and Cashmere animals with a very low frequency; while allele G has been found in all but two (Garganica and Cashmere) breeds. Alleles C, E and F were not present in the material used for this study.
Lucrari Stiintifice : Zootehnie si Biotehnologii , 2007,
Abstract: Caseins are a family of milk proteins that exist in several molecular forms and arethe main proteins present in the bovine milk. Genetic variation of these proteins hasbeen associated with the quality and quantity of cheese derived from milk.This study was focused on possibilities to evaluate the frequency of the K-casein Ballele in dairy herds from the Research and Development Station for Bovine RaisingArad in order to have breeding programs that target an increase in the frequency ofthe B allele in the dairy cattle population.In order to differentiate the favorable genotype for superior composition and highercheese yield, we used simple DNA extraction method from fresh blood andtechniques based on DNA analysis, which include polymerase chain reaction andrestriction fragment length polymorphisms (PCR-RFLP) methods. Employing thesetechniques we were able to determine the k-casein genotype of all individuals in agiven population under selection, regardless of sex, age or physiological stage.As a result, it is now possible to include information on milk protein genotypes intomarker assisted selection programs and consequently improve response to selection.
Genetic polymorphism of casein alpha-S1 gene in Tunisian local Goat  [cached]
Borni Jemmali*; Mounir Kamoun; Marwa Haddar; Abderrahmene Ben Gara; Houcine Selmi; Moncef Hammami; Marouene Amraoui; Hamadi Rouissi; Rekik Boulbaba
Biomirror , 2012,
Abstract: The genetic polymorphism of the casein alpha-S1 locus wasinvestigated in Tunisian goat. Blood samples were collected from localgoat breed. Genomic DNA samples were obtained from leukocytes of75 dairy goats and regions of interest in the gene were amplifiedthrough Polymerase Chain Reaction (PCR), then evaluated in agarosegels. For better characterization of the single nucleotidepolymorphism, if exist, a PCR-Restriction Fragment LengthPolymorphism study was performed employing the endonucleaseXmnI. DNA amplification using primers produced fragments withsizes of 457 bp. The PCR products of primer (223 bp) digested byrestriction enzyme Xmn1 produced four fragments at 223 bp, 212bp,161bp and 150-bp. The results showed that local goat breed haddifferent genotypes A/A, B/C, C/C and DD. Our results revealed thatthe CSN1S1 allelic variants in tested breed showed differentgenotypes, three of them were homozygous 12.5%, 60.5% and 12.8%respectively for A/A, C/C and D/D and the other was heterozygousB/C (14.2%). Identification of different variants of the casein alpha-S1 can be used to improve milk quality of local goat breed.
Estudo do polimorfismo genético da α S1-caseína em cabras, no Estado de Pernambuco, Brasil = Study of the genetic polymorphism of the α S1-casein in goats of Pernambuco State, Brazil
Ariosto Afonso da Silva,Manoel Adri?o,George Chaves Jimenez,Madriano Christilis da Rocha Santos
Acta Scientiarum : Animal Sciences , 2007,
Abstract: O Estado de Pernambuco tem uma voca o pecuária, especialmente, para aexplora o de caprinos. Dentre as proteínas, chamadas de caseínas, a αS1-caseína foi a primeira proteína comprovada com base no polimorfismo genético. Objetivando realizar a genotipagem de cabras criadas no sert o, agreste e zona da mata do Estado de Pernambuco, por meio da técnica de PCR-RFLP, estudou-se o polimorfismo do gene da aS1-caseína. Utilizaram-se 60 animais, divididos em três grupos de 20 animais, das ra as Moxotó, Alpina Americana e SRD (Sem Ra a Definida). A extra o do DNA foi realizada com a utiliza o do protocolo fenol-clorofórmio, e o gene da aS1-caseína foi amplificado por meio da PCR (rea o da polimerase em cadeia). Em seguida, foi utilizada a enzima de restri o XmnI para obter a freqüência alélica das ra as estudadas. Encontrou-se, nos caprinos, os alelos da aS1-caseína B e D que foram predominantes para a ra a nativa Moxotó e animais SRD (100%), e os alelos C e D, para a ra a Alpina Americana(100%), concluindo-se que existem varia es genéticas para o gene da aS1-caseína do leite das ra as caprinas estudadas, embora se evidencie a proximidade genética entre a Moxotó e SRD. The Pernambuco State, has been a livestock area, mainly for thecaprine exploration. Among the proteins, called caseins, the αS1-casein was the first proved protein with base in the genetic polymorphism. To genotype goats of the “sert o”, “agreste” and “zona da mata” regions of the Brazilian State of Pernambuco, through the PCR-RFLP technique, we studied the polymorphism of the αS1-casein gene. Sixty animals were used, divided in three groups of twenty animals of the races Moxotó, American Alpine and UB (Undefined Breed). The DNA extraction was done by the phenol-chloroform protocol and the αS1-casein gene was amplified through the PCR (Polymerase Chain Reaction). Then, the restriction enzyme XmnI was used to obtain the allele frequency of the studied races. In the goats, we found the αS1-casein alleles B and D that predominantly were in the native raceMoxotó and Ub animals (100%), the alleles, C and D for the American Alpine race (100%), concluding that there are genetic variations for the αS1-casein in the milk of the goat races studied in the region, although it was observed a genetic proximity between Moxotó and UB.
Gene frequencies of caprine alpha S1 casein polymorphism in Montenegrian Balkan goat breed
Markovi? Bo?idarka,Markovi? M.,Jovanovi? S.,Krajinovi? M.
Acta Veterinaria , 2009, DOI: 10.2298/avb0906613m
Abstract: The alleles and genotypes at the locus αS1 - casein were determined for 98 goats of domestic Balkan goat breed from Montenegro distributed in two flocks. The occurrence of two alleles (A and F) and three genotype forms (AA, AF and FF) were discovered by using the method of sequencing of cDNA and part of genomic DNA of αS1 casein. High domination of the strong A allele (0.629) in comparison with the 'weak' F allele with a frequency of 0.371 was obtained in this study. The knowledge of distribution on allelic variants at the locus αS1 - casein in the population of Balkan goats will contribute to a better valorization and improving of this breed and the preservation of its genetic variability.
Polymorphism of locus DRB3.2 in populations of Creole Cattle from Northern Mexico
Fernández, Ilda G.;Ramírez, José Gonzalo Ríos;Vázquez, Amanda Gayosso;Arvizu, Raúl Ulloa;Morales, Rogelio A. Alonso;
Genetics and Molecular Biology , 2008, DOI: 10.1590/S1415-47572008005000020
Abstract: keywords : bola-drb3.2; polymorphism; creole cattle; pcr-rflp.
Kappa Casein Gene Polymorphism in Holstein Chinese Cattle  [PDF]
A. E. Hamza1,*, X. L. Wang and Z. P.Yang
Pakistan Veterinary Journal , 2010,
Abstract: Kappa casein gene polymorphism has received a considerable attention because of its correlation with milk quality, composition and technological properties. The polymorphism of kappa casein gene (K-CN) was detected in Holstein Chinese cattle. A 218 bp sequence in exon IV of 319 Holstein Chinese cattle blood samples were amplified using polymerase chain reaction-single strand conformation (PCR-SSCP) technique. Sequence analysis revealed one single nucleotide polymorphism (SNP) T/C SNP in exon 1V at nucleotides (80), moreover; three genotypes TT, TC and CC were also identified with following frequencies: 0.40, 0.34 and 0.26%, respectively. The allele frequency for T and C found to be 0.6 and 0.4 %, respectively. Allele frequencies in the population fitted with Hardy–Weinberg equilibrium (P>0.05). Analysis of genetic polymorphism of k-casein at exon 1V exhibited medium polymorphism information content (PIC=0.36).
Genotyping of Kappa-Casein Locus by PCR-RFLP in Brown Swiss Cattle Breed
Unsal Dogru,Memis Ozdemir
Journal of Animal and Veterinary Advances , 2012,
Abstract: A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) test was performed on DNA samples extracted from blood samples of Brown Swiss cattle to detect genotype frequency of the bovine kappa-casein (CNS3) locus. A 351 bp fragment of CSN3 was amplified and digested with Hinf I restriction enzymes. Samples were loaded on agarose jel (3%) and genotyped under UV light. Three genotypes were observed, frequencies were 19.35, 20.43 and 60.22% for AA, BB and AB, respectively. Two genetic variants CSN3 A and CNS3 B were identified and the allelic frequencies were estimated as 0.495 and 0.505, respectively. It was concluded that the population was in Hardy-Weinberg equilibrum.
Short communication: Polymorphism of the GnRHR gene and its association with litter size in Boer goats
W Yang, K Tang, C Zhang, D Xu, Q Wen, L Yang
South African Journal of Animal Science , 2011,
Abstract: The polymorphism in the gonadotropin-releasing hormone receptor (GnRHR) gene was detected by PCR single-strand conformational (PCR-SSCP) and DNA sequencing methods in 224 Boer goats. Two single nucleotide polymorphisms (SNPs), G891T and G757A, were found in the exon I of the goat GnRHR gene and their associations with litter size in Boer goats were evaluated. Association analysis showed that G891T and G757A had significant effects on litter size. In locus G891T, individuals with GT genotype had significantly larger litter sizes compared to GG genotype in the first and third parity. In locus G757A, ewes with GG genotype had significantly higher litter sizes than those of GA in the fourth parity. The present study primarily revealed an association between allele T at 891 locus of the GnRHR gene and high litter size in the first and third parity in Boer goats. These results provide evidence that the GnRHR gene may be significantly correlated with a high prolificacy in goats.
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