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Non-radioisotopic method for thein vitro measurement of EGF receptor tyrosine kinase
Gaoxiang Ge,Jing Wu,Qishui Lin
Chinese Science Bulletin , 2001, DOI: 10.1007/BF03182836
Abstract: A non-radioisotopic method was developed for the assay of epidermal growth factor receptor (EGFR). A peptide with twenty amino acid residues around Tyr 1173, the major phosphorylation site of EGFR, was cloned as a GST fusion protein and used as substrate. Anti-phosphotyrosine monoclonal antibody PY99 was used for the determination of the extent of phosphorylation. Both the specificity and the sensitivity were substantially higher than that of the existing method. Km value of the fusion protein is much lower (10 μmol/L) than that of the synthetic peptide (110 μmol/L). The method can be applied to the measurement of the tyrosine kinase activity of c-erb B2 (Neu/HER2).
Non-radioisotopic method for the in vitro measurement of EGF receptor tyrosine kinase

GE Gaoxiang,WU Jing,LIN Qishui,

科学通报(英文版) , 2001,
Abstract: A non-radioisotopic method was developed for the assay of epidermal growth factor receptor (EGFR). A peptide with twenty amino acid residues around Tyr 1173, the major phosphorylation site of EGFR, was cloned as a GST fusion protein and used as substrate. Anti-phosphoty-rosine monoclonal antibody PY99 was used for the determination of the extent of phosphorylation. Both the specificity and the sensitivity were substantially higher than that of the existing method. Km value of the fusion protein is much lower (10 μmol/L) than that of the synthetic peptide (110 μmol/L). The method can be applied to the measurement of the tyrosine kinase activity of c-erb B2 (Neu/HER2).
体外循环后新西兰兔的睡眠-觉醒节律
Sleep-wake rhythm after extracorporeal circulation in New Zealand rabbits
 [PDF]

王开伟,张加强
WANG Kaiwei
, ZHANG Jiaqiang

- , 2018, DOI: 10.11817/j.issn.1672-7347.2018.04.016
Abstract: 目的:观察体外循环(extracorporeal circulation,ECC)后新西兰兔睡眠-觉醒节律的改变,探讨钟基因表达异 常在ECC导致的睡眠-觉醒节律紊乱中的作用。方法:将54只新西兰兔随机分为正常对照组(N组,n=6)、假手术组(S 组,n=24)和ECC组(n=24)。用多用途脑电记录仪分别记录3组新西兰兔皮层电图(electrocorticogram,ECOG)、眼电图 (electroophthalmogram,EOG)、肌电图(electromyogram,EMG)和兔的睡眠-觉醒节律。用半定量反转录PCR和Western 印迹法分别测定3组新西兰兔松果体中period 1(Per1)和cryptochrome 1 (Cry1)的mRNA以及Per1和Cry1的蛋白表达水平, 并比较3组之间的差异。 结果:1)分别与N组和S组的24和48 h相比,ECC组24和48 h兔睡眠总时间、浅睡眠和慢波睡 眠(slow wave sleep,SWS)各期持续的时间均显著缩短(均P<0.05),浅睡眠所占比例显著增加(均P<0.05),SWS时相所占 比例显著减少(均P<0.05)。2)与N组和S组相比,ECC组Per1 mRNA的表达水平在ECC模型制备后24和48 h明显升高(均 P<0.05),Cry1 mRNA的表达水平在ECC模型制备后24 h明显升高(均P<0.05)。3)与N组和S组相比, ECC组Per1的蛋白 水平在ECC模型制备后48 h显著升高(均P<0.05),Cry1的蛋白水平在ECC模型制备后24 h显著升高(均P<0.05)。4) ECC 组新西兰兔睡眠-觉醒节律紊乱和钟基因表达的异常在术后72 h有所改善。结论:ECC可导致新西兰兔睡眠-觉醒节律 发生紊乱,其机制可能与钟基因Cry1和Per1及其转录蛋白的表达异常有关
Ischemic Tolerance in Uremic Rabbits  [PDF]
John G. Kingma, Guylaine Sénéchal, Jacques R. Rouleau, Iris Kingma
World Journal of Cardiovascular Diseases (WJCD) , 2015, DOI: 10.4236/wjcd.2015.512041
Abstract: Background: Cardiac complications after myocardial infarction are believed to be worse in the presence of comorbidities; we tested whether experimentally induced prolonged uremia exacer-bated myocardial necrosis in a rabbit preparation of ischemia-reperfusion injury. In addition, we examined if treatment with an angiotensin converting enzyme inhibitor (Enalapril, ENA, 3 mg/Kg, IV) could reduce post-ischemic myocardial damage. Methods: Prolonged uremia was induced by a two-stage subtotal nephrectomy and confirmed by marked increases in serum creatinine and urea levels; after 5 weeks, four groups of rabbits were exposed to 45-min acute coronary occlusion followed by 180-min reperfusion. In treated animals, ENA was administered 5-min before onset of coronary reperfusion. All data from uremic animals were compared with time-matched controls. Results: Cardiac hemodynamics was similar for all groups during the development of kidney failure; heart rate in uremic rabbits was significantly lower for the duration of ischemia-reperfusion. In this animal model, the absence of coronary collateral circulation provides a stable ischemic substrate for evaluation of cellular necrosis. Infarct size (expressed as percent risk zone size) was: control, 48 ± 16; uremia, 36 ± 5; control + ENA, 51 ± 19; and uremia + ENA, 41 ± 16; risk zone size was similar for all animals. Conclusion: The present findings are inconsistent with the view that post-ischemic cardiac injury is greater in animals with pre-existent uremia. In addition, we were unable to show a significant beneficial effect with an angiotensin converting enzyme inhibitor on infarct size in either control or uremic rabbits. It remains to be proven in animal models with comorbidities such as manifest kidney disease that ischemic tolerance can be substantially reduced by either pharmacologic or non-pharmacologic interventions.
Comparative study for the identification of myocardial ischemia by contrast ventriculography under the effect of isosorbide mononitrate and by perfusional myocardial scintigraphy in patients with ischemic heart disease
Manfroi, Waldomiro Carlos;Ludwig, Roberto;Kirschnick, Luciana Schmidt;Souza, Josiane;Ribeiro, Letícia Weiss;Ordovás, Karen;Leit?o, Cristiane;Cruz, Rosana;Candiago, Rafael;
Arquivos Brasileiros de Cardiologia , 1999, DOI: 10.1590/S0066-782X1999000200005
Abstract: objective: to evaluate and compare the usefulness of cineventriculographies, before and after nitrate use, to technetium-99m sestamibi scintigraphy for the identification of myocardial ischemia. methods: twenty-six patients were studied at basal conditions and 5 minutes after intravenous administration of isosorbide mononitrate (0.3mg/kg), to evaluate the performance and regional wall motion of the left ventricle (lv). the results were compared to those obtained with technetium-99m sestamibi scintigraphy. results: before nitrate, contrast ventriculography identified 30 normal segments, 62 hypokinetic segments, 28 dyskinetic segments, and 14 akinetic segments. after drug administration, 99 segments were normal, 11 hypokinetic, 11 dyskinetic, and 13 akinetic. myocardial scintigraphy revealed 110 ischemic segments and 18 fibrotic segments (p<0.005). after drug administration, the ventriculography showed increase in the velocity of circumferential fiber shortening (p=0.0142), the ejection fraction (p=0.0462), decrease in the end-systolic volume (p=0.0031) and no change in end-diastolic volume. conclusion: contrast ventriculography using nitrate proved to be similar to perfusional myocardial scintigraphy in the identification of myocardial ischemia.
Comparative study for the identification of myocardial ischemia by contrast ventriculography under the effect of isosorbide mononitrate and by perfusional myocardial scintigraphy in patients with ischemic heart disease
Manfroi Waldomiro Carlos,Ludwig Roberto,Kirschnick Luciana Schmidt,Souza Josiane
Arquivos Brasileiros de Cardiologia , 1999,
Abstract: OBJECTIVE: To evaluate and compare the usefulness of cineventriculographies, before and after nitrate use, to technetium-99m sestamibi scintigraphy for the identification of myocardial ischemia. METHODS: Twenty-six patients were studied at basal conditions and 5 minutes after intravenous administration of isosorbide mononitrate (0.3mg/kg), to evaluate the performance and regional wall motion of the left ventricle (LV). The results were compared to those obtained with technetium-99m sestamibi scintigraphy. RESULTS: Before nitrate, contrast ventriculography identified 30 normal segments, 62 hypokinetic segments, 28 dyskinetic segments, and 14 akinetic segments. After drug administration, 99 segments were normal, 11 hypokinetic, 11 dyskinetic, and 13 akinetic. Myocardial scintigraphy revealed 110 ischemic segments and 18 fibrotic segments (p<0.005). After drug administration, the ventriculography showed increase in the velocity of circumferential fiber shortening (p=0.0142), the ejection fraction (p=0.0462), decrease in the end-systolic volume (p=0.0031) and no change in end-diastolic volume. CONCLUSION: Contrast ventriculography using nitrate proved to be similar to perfusional myocardial scintigraphy in the identification of myocardial ischemia.
Progressive Symmetric Erythrokeratoderma  [cached]
Verma Rajesh,Jaiswal A. K,Vaishampayan S. S,Baveja S
Indian Journal of Dermatology , 1999,
Abstract: A case of progressive symmetric erythrokeratoderma is reported for its rarity and clinical interest.
Process Optimization of Effective Partition Constant in Progressive Freeze Concentration of Wastewater  [PDF]
Mazura Jusoh, Anwar Johari, Norzita Ngadi, Zaki Yamani Zakaria
Advances in Chemical Engineering and Science (ACES) , 2013, DOI: 10.4236/aces.2013.34036
Abstract: Response surface methodology (RSM) was employed to optimize the process parameters for effective partition constant (K) in progressive freeze concentration (PFC) of wastewater. The effects of coolant temperature, circulation flowrate, initial solution concentration and circulation time on the effective partition constant were observed. Results show that the data were adequately fitted into a second-order polynomial model. The linear and quadratic of independent variables, coolant temperature, circulation flowrate, initial solution concentration and circulation time as well as their interactions have significant effects on the effective partition constant. It was predicted that the optimum process parameters within the experimental ranges for the best K would be with coolant temperature of -8.8℃, circulation flowrate of 1051.1 ml/min, initial solution concentration of 6.59 mg/ml and circulation time of 13.9 minutes. Under these conditions, the effective partition constant is predicted to be 0.17.
Device for craniocervical isolation in rabbits
Ivo, Cláudio Santana;Ivo, Marcelo Braga;Salles, Paulo Guilherme de Oliveira;Rosário, Ricardo Costa Val do;Nunes, Tarcizo Afonso;
Acta Cirurgica Brasileira , 2009, DOI: 10.1590/S0102-86502009000400012
Abstract: purpose: to evaluate the craniocervical isolation collar, for use in rabbits, with regard to the following aspects: effectiveness of the craniocervical attachment for up to 120 hours; ease of handling; freedom of the animal to move about and ingest food; and the physical integrity of the animal during and after use of the device. methods: starting from an elizabethan collar, a craniocervical collar was prepared for use in rabbits with the following modifications: a wide cut in the device in the shape of a half-moon, coinciding with the animal's mouth; inner division of the device into two compartments to individually isolate and immobilize the ears; multiple perforations in the upper compartment for better ventilation; adaptation of the cervical-thoracic band to attach the collar to the cervical region, keeping the fasteners on the animal's back. the device was used on 18 male new zealand rabbits for up to 120 consecutive hours. results: the device was effective in the craniocervical isolation and separation of ears in rabbits without interfering with the animals' respiration, ability to swallow food, or causing physical trauma, while allowing the animals to move about freely and rest comfortably. attaching and removing the device was easy and quick and it remained in place for up to 120 hours without the need to reposition it. conclusion: the modified craniocervical isolation collar made it possible to conduct the experiment with isolation of the rabbits' ears, ease of handling, and without causing limitations in relation to the animals' ability to eat and move about freely.
Pharmacological vasodilatation increased pulse pressure variation mimicking hypovolemic status in rabbits
GA Westphal, ARR Gon?alves, A Bedin, R Steglich, E Silva, LF Poli de Figueiredo
Critical Care , 2007, DOI: 10.1186/cc5796
Abstract: To test the hypothesis that PPV amplification observed in hypovolemia can also be observed during pharmacological vasodilatation, induced by sodium nitroprusside (SN).Ten anesthetized, mechanically ventilated rabbits, underwent progressive hypotension by either controlled hemorrhage (CH) or intravenous SN infusion. CH group: five rabbits were submitted to graded hemorrhage of 10%, 20%, 30%, 40% and 50% of their blood volume. Mean arterial pressure steps were registered and assumed as pressure targets. SN group: five rabbits were submitted to a progressive SN dose infusion to reach similar pressure targets observed in the CH group (Table 1). PPV was measured at each arterial pressure step.The heart rate was significantly greater in the SN group than in the CH group (P < 0.05). PPVs were similar among the experimental models in all steps (P = 0.17).Pharmacologic vasodilatation by SN induced a PPV amplification similar to that observed in hypovolemia. Our results reinforce the idea that PPV amplification may be associated with potential cardiovascular response and not necessarily hypovolemic status. Hence, caution should be exercised before assuming that PPV is a marker of intravascular volume status.
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