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STEVIOSIDE SYNTHESIS IN STEVIA (Stevia rebaudiana Bert) STEVIOSIDE SYNTHESIS IN STEVIA (Stevia rebaudiana Bert)  [cached]
Jarma Orozco Alfredo,Espitia Camacho Miguel,Fischer Gerhard
Acta Biológica Colombiana , 2010,
Abstract: Stevia rebaudiana Bert. is a subtropical wild plant of Paraguay, that possesses a potent sweetener up to 300 times higher than sucrose and has no calories. The molecules responsible for these characteristics are diterpen glycosides, found in leaves and synthesized at least, at initial states, which use the same pathway of the gibberellic acid in which the shiquimic acid gives origin to many aromatic compounds. The acetate is the precursor of the terpens or isoprenoids through the acetate-mevalonate pathway, where steviosides are found. Although in this article, an alternative route is discussed. This updating pretends to contribute tools for the understanding of the main pathways of steviol glycosides synthesis. Stevia rebaudiana Bert. is a subtropical wild plant of Paraguay, that possesses a potent sweetener up to 300 times higher than sucrose and has no calories. The molecules responsible for these characteristics are diterpen glycosides, found in leaves and synthesized at least, at initial states, which use the same pathway of the gibberellic acid in which the shiquimic acid gives origin to many aromatic compounds. The acetate is the precursor of the terpens or isoprenoids through the acetate-mevalonate pathway, where steviosides are found. Although in this article, an alternative route is discussed. This updating pretends to contribute tools for the understanding of the main pathways of steviol glycosides synthesis.
In vitro propagation of Stevia rebaudiana Bert in Bangladesh
MS Uddin, MSH Chowdhury, MMMH Khan, MB Uddin, R Ahmed, MdA Baten
African Journal of Biotechnology , 2006,
Abstract: An experiment was conducted on in vitro culture of Stevia rebaudiana Bert, an important non-caloric sweetening herb to explore its potential for micro-propagation. Leaf, nodal and inter-nodal segments of the selected herb as explant were cultured on MS medium containing 2,4-D at 2, 3, 4 and 5 mg/L for callus induction. Inter-nodal segments initiated callus earlier than node and leaf. The highest amount of callus was found in MS medium with 3.0 mg/L 2,4-D and MS medium with 5.0 mg/L 2,4-D gave the poorest callus
Memory Enhancing Effect of Ethanolic Extract of Stevia rebaudiana (Bert.)  [cached]
Divya S Juyal,Ganga Bisht,Arun Kumar
International Journal of Phytomedicine , 2011,
Abstract: Suspension of ethanolic extract of leaves of Stevia rebaudiana bert. in gum acacia were administered orally to evaluate the memory enhancing activity in the aged rats. Memory enhancing effect was evaluated by using Morris water maze and elevated plus maze. Mean escape latency time (ELT) calculated each day during acquisition trial was used as an index of acquisition and mean time spent in target quadrant in search of missing platform provided an index of retrieval. Significant reduction in transfer latency (TL) value of retention indicated improvement of memory. Significant memory enhancing effect of ethanolic extract Stevia rebaudiana was observed in aged rats at 200 mg/kg p.o. dose. Keywords: Stevia rebaudiana, Memory, Alzheimer disease,
EFFECT OF Stevia rebaudiana (Bert.) EXTRACT ON MEMORY AND
Divya S Juyal,Ganga Bisht,arun kumar
Journal of Global Pharma Technology , 2010, DOI: 10.1234/jgpt.v2i5.173
Abstract: The present study was designed to investigate the effect of Stevia rebaudiana in age related cognition in old and young rats. Stevia rebaudiana extract (50, 100, 200mg/kg) was administered orally in old and young rats for sixteen days and were evaluated on Morris water maze, elevated plus maze and change in cholinesterase activity. Mean escape latency time (ELT) calculated each day during acquisition trial was used as an index of acquisition and mean time spent in target quadrant in search of missing platform provided an index of retrieval. Significant reduction in transfer latency (TL) value of retention indicated the improvement of memory. Significant memory enhancing and cholinesterase effect of aqueous extract Stevia rebaudiana was observed in aged rats at 200 mg/kg p.o. dose.
SINTESIS DE ESTEVIOSIDOS EN ESTEVIA (Stevia rebaudiana Bert.)
Jarma Orozco,Alfredo; Espitia Camacho,Miguel; Fischer,Gerhard;
Acta Biológica Colombiana , 2010,
Abstract: stevia rebaudiana bert. is a subtropical wild plant of paraguay , that possesses a potent sweetener up to 300 times higher than sucrose and has no calories. the molecules responsible for these characteristics are diterpen glycosides, found in leaves and synthesized at least, at initial states, which use the same pathway of the gibberellic acid in which the shiquimic acid gives origin to many aromatic compounds. the acetate is the precursor of the terpens or isoprenoids through the acetate-mevalonate pathway, where steviosides are found. although in this article, an alternative route is discussed. this updating pretends to contribute tools for the understanding of the main pathways of steviol glycosides synthesis.
THE OPTIMAL USAGE OF GROWING BIOSTIMULATORS FOR IN VITRO MULTIPLICATION PROCESS AT STEVIA REBAUDIANA (BERT.)  [PDF]
GABRIELA ZBUGHIN,T. M. STURZU,C. TOMA
Scientific Annals of Alexandru Ioan Cuza University of Iasi. New Series, Section 2. Vegetal Biology , 2010,
Abstract: This paper presents a study about the in vitro” multiplication of Stevia rebaudiana (Bert.) species and some conclusions about the influence of growing regulators’ concentrations in mixture for some biometrics characteristics of the vitro-plants, conclusions that were drawn up using a central-compound routable programme of second degree (CCRP2). There were established optimum concentrations for the two growing regulators in the mixture that is added to Murashige and Skoog medium (1962) for obtaining the best results of the biometrics characteristics. Thus a CCRP2 could be successfully used in botany.
Pesquisa de adocantes n?o calóricos sintéticos em ado?ante natural de Stevia rebaudiana (Bert.) Bertoni
Auricchio, Mariangela T.;Batistic, Monica A.;Hoppen, Vania R.;Yamashita, Isaura Y.;
Revista Brasileira de Farmacognosia , 1989, DOI: 10.1590/S0102-695X1989000100005
Abstract: nineteen samples os stevia natural sweeteners have been analised aiming at the controlment of the presence of other synthetic sweeteners that are not declared on the label of the product. it was evidenced that in eighteen samples, saccharin was present besides the components of stevia rebaudiana (bert.) bertoni extract and that in only one of them such fact was stated on the label. aspartame was found in three of these samples and cyclamate in one of them. finally, no component of stevia rebaudiana (bert.) bertoni extract was found in one of the samples, but a mixture of manitol and aspartame was.
Development and some histochemical aspects of foliar glandular trichomes of Stevia rebaudiana (Bert.) Bert. - Asteraceae
MONTEIRO, WALKYRIA ROSSI;CASTRO, MARíLIA DE MORAES;MAZZONI-VIVEIROS, SOLANGE CRISTINA;MAHLBERG, PAUL GORDON;
Brazilian Journal of Botany , 2001, DOI: 10.1590/S0100-84042001000300013
Abstract: the ten-celled biseriate glandular trichome of stevia rebaudiana (bert.) bert.-asteraceae, found on both leaf surfaces, originates from a single protruding, protodermal cell undergoing an anticlinal division. a subsequent series of periclinal divisions, occurring in acropetal sequence, leads to the formation of the trichome, composed of five pairs of cells, one pair of basal cells, another of stalk cells and three pairs of secretory head cells. developing, still two-celled glandular trichomes already occur on leaf primordia of the second pair (these primordia measuring, in some cases, ca. 0.30 mm in length), and most of the glandular trichomes are at the mature phase on very young, expanding leaves, for example on those of the sixth pair. the secretory material released by the head cells is stored in the trichome cavity (subcuticular space). basic histochemical tests reveal that such material is lipophilic (mainly) and hydrophilic in nature.
Development and some histochemical aspects of foliar glandular trichomes of Stevia rebaudiana (Bert.) Bert. - Asteraceae
MONTEIRO WALKYRIA ROSSI,CASTRO MARíLIA DE MORAES,MAZZONI-VIVEIROS SOLANGE CRISTINA,MAHLBERG PAUL GORDON
Brazilian Journal of Botany , 2001,
Abstract: The ten-celled biseriate glandular trichome of Stevia rebaudiana (Bert.) Bert.-Asteraceae, found on both leaf surfaces, originates from a single protruding, protodermal cell undergoing an anticlinal division. A subsequent series of periclinal divisions, occurring in acropetal sequence, leads to the formation of the trichome, composed of five pairs of cells, one pair of basal cells, another of stalk cells and three pairs of secretory head cells. Developing, still two-celled glandular trichomes already occur on leaf primordia of the second pair (these primordia measuring, in some cases, ca. 0.30 mm in length), and most of the glandular trichomes are at the mature phase on very young, expanding leaves, for example on those of the sixth pair. The secretory material released by the head cells is stored in the trichome cavity (subcuticular space). Basic histochemical tests reveal that such material is lipophilic (mainly) and hydrophilic in nature.
Enhanced Development of Embryogenic Callus in Stevia rebaudiana Bert. by Additive and Amino acids  [PDF]
A. Das,N. Mandal
Biotechnology , 2010,
Abstract: Stevia rebaudiana Bert. is an economically important medicinal plants act as a sugar substitute for diabetic and obese people. In the present investigation a novel protocol was developed to accelerate the production of embryogenic calli so that large number of in vitro plant regeneration can be feasible. Two different types of amino acids (Trypthphan and Glutamine) in different concentration and three different types of additives (yeast extract, casein hydrolysate and Potato extract) were utilized along with MS medium supplemented with 2,4-D (2.0 mg L-1), BAP (0.5 mg L-1), sucrose (30 g L-1) and agar with leaf as explant to elucidate their role in developing and maintaining embryogenic calli. It was found that glutamine (50 mg L-1) and casein hydrolysate (100 mg L-1) produced greenish, healthy nodular calli with more embryogenic potential and less necrotic lesion in comparison with only PGR supplemented basal medium which further performed better in regeneration medium for producing large number of regenerated shoots.
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