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泻黄散儿科难病治验  [PDF]
宋明锁
中国中医药信息杂志 , 1999,
Abstract: 泻黄散出自宋?钱乙《小儿药证直决》。由藿香、生石膏、栀子、防风、甘草五味中药组成。原方为脾热弄舌而设,具有清泻脾胃伏火之功。笔者临证以此方灵活加减治疗小儿皮肤粘膜淋巴结综合征、乙型肝炎、过敏性紫癜等多种儿科疑难杂病,每获良效,现举验案如下。
Relative frequency of astrovirus in children suffering from gastroenteritis referred to Aboozar hospital, Ahvaz  [PDF]
Seyed Hamid Reza Mozhgani,Ali Reza Samarbaf-Zadeh,Manoochehr Makvandi,Ahmad Shamsi-Zadeh
Jundishapur Journal of Microbiology , 2011,
Abstract: Introduction and objective: Human astrovirus (HAstVs), belonging to a family of non enveloped, icosahedral RNA viruses and causes gastroenteritis both in infants and adults. The aim of this study was to determine the relative frequency of viral gastroenteritis caused by astrovirus among children less than five years referred to Ahvaz Aboozar hospital.Materials and methods: Astrovirus infection was detected with Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The authenticity of PCR products was confirmed by sequencing. Results: Astrovirus infection was detected in 29 cases of 184 (15.77%), 13 positive samples belonged to the children up to six months. The relationship between gender and the prevalence of astroviral gastroenteritis was not significant. Most cases occurred during the coldest months of the year. After the sequencing, genotypes eight and four were the dominant types in this study.Conclusion: It was shown that human astrovirus plays an important role in gastroenteritis in Ahvaz, south west of Iran. The prevalence of the infection was very high. To decrease prevalence of astroviral infection, education and personal hygiene is advised.
Adenovirus, calicivirus and astrovirus detection in fecal samples of hospitalized children with acute gastroenteritis from Campo Grande, MS, Brazil
Andreasi, Marcia Sueli Assis;Cardoso, Divina das Dores de Paula;Fernandes, Sonia Maria;Tozetti, Ines Aparecida;Borges, Ana Maria Tavares;Fiaccadori, Fabíola Souza;Santos, Rodrigo Alessandro T?go;Souza, Menira;
Memórias do Instituto Oswaldo Cruz , 2008, DOI: 10.1590/S0074-02762008000700020
Abstract: we analyzed fecal samples from hospitalized children up to three years of age with acute gastroenteritis at campo grande, mato grosso do sul, brazil, from may 2000-january 2004. astrovirus and calicivirus were detected by reverse transcription-polymerase chain reaction and adenovirus was detected using the rotavirus and adenovirus combined immunoenzyme assay. astrovirus, adenovirus and calicivirus were detected at rates of 3.1%, 3.6% and 7.6%, respectively. these results re-emphasize the need for the establishment of regional vigilance systems to evaluate the impact of enteric viruses on viral gastroenteritis.
Astrovirus and Rotavirus Co-Infections in Children With Gastroenteritis who Were Referred to Ahvaz Aboozar Hospital, Southern Iran  [cached]
Seyed Hamid Reza Mozhgani,Ali Reza Samarbaf-Zadeh,Manoochehr Makvandi,GholamReza Kalvandi
Jundishapur Journal of Microbiology , 2012,
Abstract: Background: Acute gastroenteritis, which is one of the most common diseases in humans,isresponsible for many illnesses in both children and adults. Group A rotaviruses are considered the main agents of gastroenteritis, and these are followed by calciviruses, adenoviruses,and astroviruses.Objectives: The aim of this study was to determine the rate of astrovirus and rotavirus coinfection among children up to 5 years of age who had gastroenteritis and who were referred to Ahvaz Aboozar Hospital.Patients and Methods: A total of 180 stool specimens, which were collected from children with gastroenteritis who were less than 5 years old and who were referred to Ahvaz Aboozar Hospital, were tested by enzyme-linked immunosorbent assay methods for the detection of rotavirus infections. Detection of astroviruses in positive rotavirus stool specimens was performed by reverse transcriptase-polymerase chain reaction (RT-PCR) methods. Results: Fifty-nine of the 180 samples were positive for rotavirus infection. These positive samples were subjected to RT-PCR to test forastrovirus. After RT-PCR with specific astrovirus primer sets, 8 samples were positive for astrovirus as well. Therefore, 13% of rotavirus-positive samples were also positive for astrovirus.Conclusions: Group A rotaviruses, in addition tocalciviruses, adenoviruses, and astroviruses,can cause acute gastroenteritis. Studies have shown that 2.5 million deaths occur every year from gastroenteritis. In this study, we found that the prevalence of rotavirus infections was very high and that of coinfections of rotavirus and astrovirus were considerable. In order to reduce the risk of infections and to eliminate viral gastroenteritis in this zone of the region, education, vaccinations, and improved personal hygiene must be improved.
Prevalence of Rotavirus, Adenovirus, and Astrovirus Infections Among Patients with Acute Gastroenteritis in, Northern Iran
R Hamkar,Y Yahyapour,M Noroozi,K Nourijelyani
Iranian Journal of Public Health , 2010,
Abstract: Background: The aim of the study was to determine the incidence of non-bacterial acute gastroenteritis associated with diar-rheal diseases in Mazandaran Province, northern Iran.Methods: A total of 400 symptomatic cases from patients with acute gastroenteritis from Mazandaran Province in Iran were screened using EIA method for the presence of rotavirus, adenovirus and astrovirus during 2005-2006. Chi-square tests were used for testing relationships between different variables.Results: Rotavirus, adenovirus and astrovirus were detected in 62%, 2.3%, and 3% of samples, respectively. The maximum rate of rotaviruses was detected in the <1-year-old age group, while minimum rate was found in the 10 years and older age group. Astrovirus and adenovirus were detected predominantly in the 2-5-year-old age group of children, with a prevalence of 8.3% and 3.5% respectively. All studied viral gastroenteritis peaked in the winter, and minimum rate were found in summer.Conclusion: Our statistical analyzes indicated that viral gastroenteritis, especially Rota-viral, had the highest number of occurrences in colder seasons notably in winter and more frequently were observed among younger children.
Complete genome sequence of an astrovirus identified in a domestic rabbit (Oryctolagus cuniculus) with gastroenteritis  [cached]
Stenglein Mark D,Velazquez Eric,Greenacre Cheryl,Wilkes Rebecca P
Virology Journal , 2012, DOI: 10.1186/1743-422x-9-216
Abstract: A colony of domestic rabbits in Tennessee, USA, experienced a high-mortality (~90%) outbreak of enterocolitis. The clinical characteristics were one to six days of lethargy, bloating, and diarrhea, followed by death. Heavy intestinal coccidial load was a consistent finding as was mucoid enteropathy with cecal impaction. Preliminary analysis by electron microscopy revealed the presence of virus-like particles in the stool of one of the affected rabbits. Analysis using the Virochip, a viral detection microarray, suggested the presence of an astrovirus, and follow-up PCR and sequence determination revealed a previously uncharacterized member of that family. Metagenomic sequencing enabled the recovery of the complete viral genome, which contains the characteristic attributes of astrovirus genomes. Attempts to propagate the virus in tissue culture have yet to succeed. Although astroviruses cause gastroenteric disease in other mammals, the pathogenicity of this virus and the relationship to this outbreak remains to be determined. This study therefore defines a viral species and a potential rabbit pathogen.
An outbreak of gastroenteritis associated with astrovirus serotype 1 in a day care center, in Rio de Janeiro, Brazil
Silva, AMV;Leite, EG;Assis, RMS;Majerowicz, S;Leite, JPG;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000800007
Abstract: between june 4th and june 20th 1996 rotavirus, adenovirus, and astrovirus (hastrv) were investigated in fecal samples from 27 children under three years old with acute diarrhea, attending the bertha lutz day care center, in rio de janeiro. all fecal samples were analyzed by polyacrylamide gel electrophoresis (page), reverse transcriptase polymerase chain reaction (rt-pcr), enzyme immunoassays (eia), and electron microscopy (em). nine of them (33%) showed positive results for hastrv by at least one of the employed methodologies. eight were positive by rt-pcr and eia, and six by em. all positive samples were inoculated onto ht-29 (human colon adenocarcinoma) cultured cells for hastrv isolation and seven were positive after three passages. the sequencing analysis of eight rt-pcr products (449 bp) from gene that codifies vp2 protein, showed a total nucleotide identity among them and 98% with hastrv-1 (strain oxford type 1). this is the first report of a gastroenteritis outbreak associated with hastrv-1 in a day care center in rio de janeiro and it reinforces the importance of this virus in association with infantile acute gastroenteritis.
An outbreak of gastroenteritis associated with astrovirus serotype 1 in a day care center, in Rio de Janeiro, Brazil  [cached]
Silva AMV,Leite EG,Assis RMS,Majerowicz S
Memórias do Instituto Oswaldo Cruz , 2001,
Abstract: Between June 4th and June 20th1996 rotavirus, adenovirus, and astrovirus (HAstrV) were investigated in fecal samples from 27 children under three years old with acute diarrhea, attending the Bertha Lutz day care center, in Rio de Janeiro. All fecal samples were analyzed by polyacrylamide gel electrophoresis (PAGE), reverse transcriptase polymerase chain reaction (RT-PCR), enzyme immunoassays (EIA), and electron microscopy (EM). Nine of them (33%) showed positive results for HAstrV by at least one of the employed methodologies. Eight were positive by RT-PCR and EIA, and six by EM. All positive samples were inoculated onto HT-29 (human colon adenocarcinoma) cultured cells for HAstrV isolation and seven were positive after three passages. The sequencing analysis of eight RT-PCR products (449 bp) from gene that codifies VP2 protein, showed a total nucleotide identity among them and 98% with HAstrV-1 (strain Oxford type 1). This is the first report of a gastroenteritis outbreak associated with HAstrv-1 in a day care center in Rio de Janeiro and it reinforces the importance of this virus in association with infantile acute gastroenteritis.
白粉菌诱导下感病小麦京411中TCTP基因的表达分析  [PDF]
郑舒扬,王艳红,肖莹,刘晓颖,王振英
生物技术通报 , 2014,
Abstract: TCTP广泛存在于动植物及微生物中,参与重要的生物过程。对小麦中克隆到的TCTP基因进行了白粉菌诱导下的表达模式分析,结果发现,在对白粉菌敏感的小麦京411中,白粉菌侵染后TCTP基因应答迅速,且基因表达水平较高。用病毒介导的基因沉默方法沉默京411中的TCTP基因,发现白粉菌成功侵染小麦叶片的比例下降,乳突等抗性结构特征比例上升,H2在细胞内的累积水平升高。试验结果表明,TCTP在小麦与白粉菌互作过程中起重要作用。
龙胆泻肝汤治疗消化系疾病体会  [PDF]
孙昌宏
中国中医药信息杂志 , 2000,
Abstract: 龙胆泻引汤有泻引胆实火,清下焦湿热的功用,主治引胆实火上炎,或湿热下注一类的病证.笔者用此方治疗消化系疾病取得满意效果,现举例报道如下.
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