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Clinical Evaluation of A New Pathological Classification for ?Non-small Cell Lung Cancer Based on Histogenesis of Bronchial Tree  [PDF]
Nana WANG, Nan JIANG, Xiaoqing YANG, Lei FANG, Qing SUN
- , 2018, DOI: : 10.3779/j.issn.1009-3419.2018.07.05
Abstract: Background and objective Due in part to remarkable advances over the past decade in our understanding of lung cancer, there is a pressing need for a revised classification. The aim of this study was to explore and verify the clinical significance of a new molecular classification of non-small cell lung cancer (NSCLC) on the basis of anatomic site of bronchial tree. Methods Double-immunohistochemistry were performed in 105 tumor samples paring with normal lung tissue from non-small cell lung cancer patients by using monoclonal antibody of P63/NapsinA and TTF-1/CK7. By comprehensively analyzing the express profiles of tumors and normal lung tissues, histological characteristics we proposed a brand new pathological classification based on histogenesis for NSCLC and divided them as: bronchiole epithelial cell carcinoma, bronchiole-alveolar cell carcinoma, alveolar cell carcinoma and secretory adenocarcinoma. And to analyze the relationship between this classification and epidermal growth factor receptor (EGFR) mutation and ognosis. Results Further investigation revealed that our new classification showed strong relevancy with EGFR mutations and effective indicators for prognosis. Conclusion The classification system for NSCLC proposed by our research group is convenient for pathological diagnosis and valuable in clinical application.?
Histological chorioamnionitis and lung damage in preterm newborns
Rocha,Gustavo; Proen?a,Elisa; Quintas,Concei??o; Rodrigues,Teresa; Guimar?es,Hercília;
Arquivos de Medicina , 2005,
Abstract: introduction: clinical and experimental data suggest that exposure to intrauterine infection is associated not only to lung maturation and reduced risk of respiratory distress syndrome, but also with delayed alveolarization and increased risk of broncopulmonary dysplasia. e aimed to evaluate the association between histological chorioamnionitis and lung disease in preterm newborns. methods: a retrospective study of 452 neonates less than 34 weeks gestational age, delivered at three tertiary medical centers in the north of portugal, between 2001 and 2002. the association between histological chorioamnionitis and lung damage (respiratory distress syndrome and bronchopulmonary dysplasia) was evaluated through the calculation of odds ratio. results: one hundred and twenty five newborns from mothers with histological chorioamnionitis and 327 without the condition. the association between histological chorioamnionitis and respiratory distress syndrome was or 1.5 (95% ci 0.94 - 2.31). the association between chorioamnionitis and bronchopulmonary dysplasia was or 2.6 (95% ci 1.16 -6.03). the association between histological chorioamnionitis and bronchopulmonary dysplasia when adjusted for gestational age and small for gestational age revealed no statistical significance: or 1.2 (95% ci 0.51 - 2.95) for any placental finding and or 1.4 (95% ci 0.46 - 4.09) for funisitis and/ or vasculitis. conclusion: in this study we could not confirm a decrease of respiratory distress syndrome in neonates with histological chorioamnionitis nor an association to increased risk for bronchopulmonary dysplasia, as described in some studies.
Expression and Significance of bag-1, bcl-2 in Non-small Cell Lung Cancer and the Correlation with Multi-drug Resistance  [PDF]
Jiabao MA,Wen ZHU,Qinghua ZHOU
Chinese Journal of Lung Cancer , 2009,
Abstract: Background and objective bag-1, bcl-2 and bax are all apoptosis-related proteins. They play a role in the diagnosis, progress, metastasis and prognosis of tumor. The aim of the study was to investigate the expression of bag-1, bcl-2 and bax in non-small cell lung cancer, and to study the relationship between their expression levels and the clinical pathological characteristics, furthermore, to evaluate their correlation with multi-drug resistance. Methods The expressions of bag-1, bcl-2 and bax in 140 non-small cell lung cancer tissues (40 of 140 were processed neoadjuvant chemotherapy) and 15 lung benign lesion tissues were examined with SP immuno-histochemical stain. Results The positive expression rates of bag-1 and bcl-2 protein in non-small cell lung cancer were significantly higher than those in pulmonary benign lesion tissues (P < 0.05), but the positive expression rate of bax in non-small cell lung cancer was significantly lower than that in pulmonary benign lesion tissues (P < 0.05). The expressions of bag-1, bcl-2 and bax protein were not related to the age and sex of patients, histological classification, P-TNM stage and lymph node involvement of the cancer (P > 0.05), but bag-1 was related to the differentiation degree of the tumor. The lower the differentiation was, the higher the levels of expression of bag-1 were. bcl-2 protein expression was highly positive correlated with the bag-1 protein expression in non-small cell lung cancer (r =0.371, P < 0.01), and bcl-2 protein was highly negative correlated with bax protein expression (r=-0.225, P < 0.01). The positive expression rates of bag-1 and bcl-2 showed increasing trends from the patients without neoadjuvant therapy to those with neoadjuvant therapy, but the difference had no statistic significance (P > 0.05). Conclusion The high expression of bag-1, bcl-2 protein and the low expression of bax protein exist in nonsmall cell lung cancer. The expression level of bag-1 protein is closely related to the differentiation degree of non-small cell lung cancer. A highly positive correlation exists between bag-1 and bcl-2 expression, and a highly negative correlation is observed between bcl-2 and bax expression. The study doesn’t provide the evidence that there is a close correlation between the expression levels of bag-1, bcl-2, bax and the multi-drug resistance in non-small cell lung cancer.
hnRNP K Expression and Its Clinical Significance in Human Lung Cancer Tissues  [cached]
Yan CHEN,Weimin LI,Shangfu ZHANG
Chinese Journal of Lung Cancer , 2008,
Abstract: Background and objective There is no any specific criterion in early diagnosis of lung cancer yet. The aim of this study is to determine hnRNP K expression in lung cancer tissues and normal lung tissues and to provide data for the diagnosis and treatment of lung cancer. Methods The expression of hnRNP K was studied by using the SP method of immunohistochemistry in lung tissue samples of 70 cases of lung cancer and 27 cases of control groups. Results The positive rate of hnRNP K was 64.9% (76/117) in lung cancer tissues, which was significantly higher than that in control group (33.3%, 9/27) (P<0.001). hnRNP K was localized in the cytoplasm and/ or nucleus. Three subtypes of lung cancer all showed positive staining of hnRNP K protein. The positive rate of hnRNP K was 73.7% (14/19) in SCLC and 54.9% (28/51) in NSCLC (P>0.05). Conclusion hnRNP K can highly express in lung cancer tissues, but it does not correlate with histological classification of lung cancer.
Prognostic significance of tympanosclerotic plaques localization and their morphological and histological characteristics for the outcome of surgical treatment
Milojevi? Milanko,?eri? Dragoslava,Bijeli? Du?an
Vojnosanitetski Pregled , 2012, DOI: 10.2298/vsp100815005m
Abstract: Background/Aim. Tympanosclerosis is a sequela of inflammation of the middle ear usually causing conductive hearing loss. The aim of the study was to determine the significance of tympanosclerotic plaques localization in the middle ear and their morphological and histological characteristics for surgical treatment outcome. Methods. This retrospective study included a total of 73 patients operated on for tympanosclerosis in the Clinic for Otorhinolaryngology, Military Medical Academy (MMA) in a period 1996-2010. The results of surgical treatment as well as the last audiometry findings were analyzed. considering follow-up periods of 6 months to 8 years. The patients were divided into 4 groups according to tympanosclerotic plaques localization in the middle ear and the classification suggested by Wieling and Kerr. The patients were also divided based on intraoperatively noticed morphological characteristics of tympanosclerotic plaques, while the third division was done as per histological findings. Surgical success was assessed using the suggestions of the Japan Otological Society. Results. The analyzed results showed the surgical success especially in the group II according to Wieling and Kerr, while histological findings had no impact on the outcome of the surgery. Conclusion. Surgical treatment has good results especially in patients with the mobile stapes. Results are satisfactory in other localizations, while various morphological and histological characteristics do not have impact on the surgery outcome.
Histological types and age distribution of lung cancer operated patients over a 20-year period: A pathohistological based study  [PDF]
Stoj?i? Jelena,Ad?i? Tatjana,Mari? Dragana,Suboti? Dragan
Srpski Arhiv za Celokupno Lekarstvo , 2011, DOI: 10.2298/sarh1110619s
Abstract: Introduction. Lung cancer is the leading cause of death from malignancy in Serbia. Objective. This is a retrospective analysis of lung cancer epidemiological changes regarding to its histological type and patients’ age of both genders. Data were based on surgically treated lung cancer patients from 1985 to 2005. Methods. Data were collected from 972 pathohistological reports of operated patients of both genders divided into age groups. Histological types of lung cancer were distributed in four major groups: squamous cell cancer (SCC), adenocarcinoma (AC), small cell cancer (SCLC) and other rare histological types. Both genders together and separately were analysed. Chi-square with the level of significance p<0.05 and chi-square test for trends were used as statistical methods. Results. SCC predominated in both genders; in 44.7% females and 68.0% males. AC was less frequently diagnosed (21.8%) than SCC (64.0%) in both genders and all age groups. The most frequently operated patients were aged between 51 and 60 years (36.6%) with SCC and AC predominance. Three patients with SCLC were operated in 61-70 age-group. In age-group up to 30 years, three (0.5%) patients were operated on for SCC and other rare lung tumours, respectively. Predominance of other rare lung tumours was established in 51-60 age-group, 25% of patients of both genders. Conclusion. SCC is the most frequent histological type of lung cancer found in all age groups and in both genders of surgically treated patients.
Significance of Detection of Serum Carbonic Anhydrase IX in the Diagnosis of Lung Cancer  [PDF]
Fangyuan CHENG, Xiao’e WANG, Diansheng ZHONG, Linlin SUN, Qian WANG, Chang LIU
- , 2015, DOI: : 10.3779/j.issn.1009-3419.2015.01.05
Abstract: Background and objective Carbonic anhydrase IX (CAIX) is a transmembrane protein involved in the metabolism of tumor cells. CAIX is expressed in only a few normal tissues but is overexpressed in various tumor types. The aim of this study is to detect the serum CAIX level of patients with lung cancer, evaluate the significance of CAIX detection in the diagnosis of lung cancer, and analyze the serum CAIX level among different pathological types and TNM stages of lung cancer. Methods Forty-seven patients with lung cancer and 31 healthy subjects were selected to participate in this study. Serum CAIX level was examined through enzyme-linked immunosorbent assay (ELISA). The subjects were grouped according to histological type and TNM staging, and serum CAIX level was compared among the groups. With pathological diagnosis as the gold standard, receiver operating characteristic curve of the serum CAIX level was established for the diagnosis of lung cancer. Results The CAIX serum level was significantly higher in patients with lung cancer than that in the healthy group (P<0.001). The serum CAIX level in patients with squamous cell carcinoma and small cell carcinoma was also significantly higher than that in patients with adenocarcinoma. No statistically significant differences were observed in the serum CAIX level between I+II and III+IV staging. The AUC of serum CAIX level was 0.961. At a threshold level of 115.115 pg/mL, sensitivity and specificity were 95.7% and 90.3%, respectively. Conclusion Detection of the serum CAIX level through ELISA exhibits high sensitivity and specificity and is important for the diagnosis of lung cancer.
Influence of Fixation Products Used in the Histological Processing in the FTIR Spectra of Lung Cells
Thiago Martini Pereira,Maria Lucia Zaidan Dagli,Grégory Mennecier,Denise Maria Zezell
Spectroscopy: An International Journal , 2012, DOI: 10.1155/2012/649094
Abstract: The aim of the present study is to evaluate the differences on FTIR spectra of the normal lung cell (noncancerous mice lung epithelial cell line e10) due to different fixation protocols for histological processing. The results shown that formalin and methacarn (normally used in fixation) did cause many changes on the FTIR spectra of mice lung cells e10, mainly in the organic compounds (800–1800 cm−1) in lipids, DNA, and proteins, and the alcohol 70% fixation protocol caused almost no changes on the FTIR spectra compared to unfixed cells spectra (in PBS). It can be concluded that histological processing with alcohol 70% fixation protocol can be used in the FTIR study of mice lung cell line e10.
Detection of EGFR and COX-2 Expression by Immunohistochemical Method on a Tissue Microarray Section in Lung Cancer and Biological Significance  [PDF]
Congzhong ZHU,Juan LIU,Xinyun WANG
Chinese Journal of Lung Cancer , 2010,
Abstract: Background and objective Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2), which can regulate growth, invasion and metastasis of tumor through relevant signaling pathway, have been detected in a variety of solid tumors. The aim of this study is to investigate the biological significance of EGFR and COX-2 expression in lung cancer and the relationship between them. Methods The expression of EGFR and COX-2 was detected in 89 primary lung cancer tissues, 12 premaliganant lesions, 12 lymph node metastases, and 10 normal lung tissues as the control by immunohistochemical method on a tissue microarray section. Results EGFR protein was detectable in 59.6%, 41.7%, and 66.7% of primary lung cancer tissues, premalignant lesions and lymph node metastases, respectively; COX-2 protein was detectable in 52.8%, 41.7%, and 66.7% of primary lung cancer tissues, premalignant lesions and lymph node metastases, respectively, which were significantly higher than those of the control (P < 0.05). The positive ratios and the levels of the expression of EGFR and COX-2 proteins were closely related to histological type, clinical stage and lymph node metastasis of lung cancer (P < 0.05), but not to histological grade, sex and age (P>0.05). COX-2 expression was related to gross type (P < 0.05). A highly positive correlation was observed between EGFR and COX-2 expression (P < 0.01). Conclusion Overexpression of EGFR and COX-2 may play an important role in the tumorgenesis, progression and malignancy of lung cancer. Detection of EGFR and COX-2 expression might be helpful to diagnosis and prognosis of lung cancer.
Expression and clinical significance of matrix metalloproteinase-26 in non-small cell lung cancer  [cached]
Yang ZHANG,Cai LI,Xuexun FANG,Meihua SUN
Chinese Journal of Lung Cancer , 2008,
Abstract: Background and objective It has been proven that matrix metalloproteinase (MMP) -26 played an important role in tumorigenesis. The aim of this study is to investigate the expression of MMP-26 protein in non-small cell lung cancer (NSCLC), and to analyze the correlations between MMP-26 and clinicopathological parameters. Methods Seventy specimens of lung cancer, 30 of (para-cancer) normal lung tissues and 19 of pulmonary benign disease tissues were collected. Immunohistochemistrical technique was used to measure the levels of expression of MMP-26 protein. Results ① The expression levels of MMP-26 protein in lung cancer tissues (64.3%) were significantly higher than that in paracancerous tissues (26.7%) and pulmonary benign disease tissues (31.3%) (P<0.01). ② The expression levels of MMP-26 protein in lung cancer tissues were closely related to histological classification, grade of cell differentiation and TNM stages (P<0.05), but not related to age, sex, smoking history and tumor size (P>0.05). Conclusion The expression levels of MMP-26 protein may play an important role in early invasion of NSCLC and may be an useful biomarker in determining its progress and prognosis.
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