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Identification of cultivated strains of Auricularia auricula from northeastern China by ISSR marker
用ISSR分子标记鉴别东北地区黑木耳生产菌株的研究

ZHANG Jie-Chi,MA Qing-Fang,ZHANG Pi-Qi,DAI Xiao-Dong,HAN Zeng-Hua,KONG Xiang-Hui,
张介驰
,马庆芳,张丕奇,戴肖东,韩增华,孔祥辉

微生物学报 , 2007,
Abstract: Inter simple sequence repeat (ISSR) method was used to distinguish the strains of Auricularia auricula in northeastern China. The result showed that 10 primers selected from 20 ISSR primers tested could produce distinct and polymorphic fingerprints of 27 cultivated strains of A. auricula. The result from the clustering analysis by NTSYS showed that these cultivated strains could be divided into three groups at similar level of 0.75. The result indicated that ISSR marker was an effective tool for strain identification and fingerprint analysis of A.auricula.
Identification of cultivated strains of Auricularia auricula from northeastern China by ISSR marker
用ISSR分子标记鉴别东北地区黑木耳生产菌株的研究

ZHANG Jie-Chi,MA Qing-Fang,ZHANG Pi-Qi,DAI Xiao-Dong,HAN Zeng-Hua,KONG Xiang-Hui,
张介驰
,马庆芳,张丕奇,戴肖东,韩增华,孔祥辉

菌物学报 , 2007,
Abstract: Inter simple sequence repeat (ISSR) method was used to distinguish the strains of Auricularia auricula in northeastern China. The result showed that 10 primers selected from 20 ISSR primers tested could produce distinct and polymorphic fingerprints of 27 cultivated strains of A. auricula. The result from the clustering analysis by NTSYS showed that these cultivated strains could be divided into three groups at similar level of 0.75. The result indicated that ISSR marker was an effective tool for strain identification and fingerprint analysis of A.auricula.
The pathogens of white mildew disease of cultivated Auricularia auricula-judae in Northeast China
东北地区木耳“白毛菌病”的病原菌

KONG Xiang-Hui,LIU Jia-Ning,ZHANG Pi-Qi,DAI Xiao-Dong,HAN Zeng-Hu,MA Qing-Fang,ZHANG Jie-Chi,
孔祥辉
,刘佳宁,张丕奇,戴肖东,韩增华,马庆芳,张介驰

菌物学报 , 2011,
Abstract: The white mildew disease on Auricularia auricula-judae was epidemic in recent years in Northeast China. The pathogenic fungi formed a layer of white pull-mesh mycelium on the ventral side of the fruiting body of A. auricula-judae in hot summer. The fungi significantly reduce the yield as well as the quality of the fruiting bodies. In this study, two fungal strains were isolated from the infected fruiting bodies cultivated in Shangzhi of Heilongjiang Province. They were identified as Fusarium oxysporum and Fusarium chlamydosporum. Both of them are pathogenic to the fruiting body of A. auricula-judae.
Suitability of Selected Supplemented Substrates for Cultivation of Kenyan Native Wood Ear Mushrooms (Auricularia auricula)  [PDF]
B.O. Onyango,V.A. Palapala,P.F. Arama,S.O. Wagai
American Journal of Food Technology , 2011,
Abstract: Different organic substrates namely maize cobs, wheat straw, grass straw and sugarcane bagasse supplemented with either wheat or rice bran were evaluated for production of two Kenyan native strains of wood ear mushroom [Auricularia auricula (L. ex Hook.) Underw.]. The objective was to evaluate the suitability of these substrates for cultivation of Kenyan native wood ear mushroom. Plastic bag technology was used with treatments arranged in a completely randomized design replicated three times. Samples of black and brown strains of the wood ear mushroom collected from woody stems of dead and dying trees within Kakamega forest were used in this study. Data was collected on days to pinning, fruit body quality, fruit body yields (number and fresh weight) and biological efficiency. The data collected was subjected to analysis of variance using SAS version 9.1. Mean separation was done using LSD and effects declared significant at 5% level. The two mushroom strains were not significantly (p>0.05) different in performance except for the number of fruit bodies where the black strain yielded significantly (p<0.05) higher than the brown one. The best performance was obtained from maize cobs and wheat straw substrates supplemented with wheat bran and these combinations were recommended to wood ear mushroom growers.
The physiological characteristics and genetic diversity analysis of Auricularia auricula-judae cultivated germplasm in China
中国木耳栽培种质生物学特性及遗传多样性分析

LI Li,FAN Xiu-Zhi,XIAO Yang,ZHOU Yan,BIAN Yin-Bing,
李黎
,范秀芝,肖扬,周雁,边银丙

菌物学报 , 2010,
Abstract: The physiological characteristics of 32 main cultivated strains of Auricularia auricula-judae in China were analyzed, and the 27 indexes were evaluated and clustered by UPGMA and PCO. The results demonstrated that the cultivated germplasm of A. auricula-judae in China are richly endowed with genetic diversity, and the 32 strains analyzed are different physiologically. The strains can be divided into 3 groups, group of strains mainly cultivated in northeast region, group of strains in central and southeast regions, and group of strains in north, south and part of central regions. It is revealed that the physiological characteristics significantly correlated with geographical origins. The cultivated strains in the same region are more similar physiologically and some strains are suspected to be synonymous.
ISSR fingerprint analysis and SCAR marker of major cultivated strains of Auricularia auricula in China
中国黑木耳主要栽培菌株ISSR指纹分析及SCAR标记

TANG Li-Hu,XIAO Yang,BIAN Yin-Bing,
唐利华
,肖扬,边银丙

微生物学报 , 2008,
Abstract: Inter-simple sequence repeat(ISSR) was used to conduct DNA fingerprint analysis of thirty-four major cultivated strains of Auricularia auricula in China, and these strains primarily conducted a standard DNA fingerprint. On the base of ISSR analysis, the 34 strains were clustered by UPGMA and two specific ISSR bands from strain 173 and 186 were converted into two sequence characterized amplified region (SCAR) markers which were used to rapid strain identification. The result showed that the genetic background of cultivated strains of A. auricula was similar and nomenclature of the cultivated strains was confused and full of synonyms. Utilization of ISSR fingerprint and its SCAR marker for rapid identification of cultivated strains in A. auricula is practicable and significative.
ISSR fingerprint analysis and SCAR marker of major cultivated strains of Auricularia auricula in China
中国黑木耳主要栽培菌株ISSR指纹分析及SCAR标记

TANG Li-Hu,XIAO Yang,BIAN Yin-Bing,
唐利华
,肖扬,边银丙

菌物学报 , 2008,
Abstract: Inter-simple sequence repeat(ISSR) was used to conduct DNA fingerprint analysis of thirty-four major cultivated strains of Auricularia auricula in China, and these strains primarily conducted a standard DNA fingerprint. On the base of ISSR analysis, the 34 strains were clustered by UPGMA and two specific ISSR bands from strain 173 and 186 were converted into two sequence characterized amplified region (SCAR) markers which were used to rapid strain identification. The result showed that the genetic background of cultivated strains of A. auricula was similar and nomenclature of the cultivated strains was confused and full of synonyms. Utilization of ISSR fingerprint and its SCAR marker for rapid identification of cultivated strains in A. auricula is practicable and significative.
Ethno-Edible Mushroom of Pleurotus sp., Clytocybe nebularis and Auricularia auricula in Ranupani Village, East Java  [PDF]
Jehan Ramdani Haryati,Rodiyati Azrianingsih
Journal of Tropical Life Science , 2012,
Abstract: This paper documented the knowledge about the wild edible fungal flora of Ranupani Village of East Java Indonesia that used by indigenous Tenggerese tribes. Study was conducted by using semi-structured interview and purposive sampling. Data were analyzed quantitatively descriptive. Index of Cultural Significance (ICS) was used to evaluate the importance of non-wood forest’s yields for local people. Identification of edible mushrooms was based on Guidelines of Flora Diversity Data Collection. This study was focus on the three edible mushrooms which are eaten mostly by the villagers, i.e. Pleurotus sp., Clytocybe nebularis and Auricularia auricula. The result is the Ranupani residents’ perception of edible mushrooms and its potential as an alternative source of food based on local knowledge and local wisdom of Tenggerese. Pleurotus sp. has the highest preference and intensity to be consumed with 3,2 ICS. It means culturally, this edible mushroom were not too important but intensively used as a secondary food sources such as soup (added or sauted), chips (dried) and fried. Ranupani Villagers always consumed edible mushrooms in rainy season. The ability of residents to distinguish edible mushrooms are equal to the searching ability to find edible mushroom substrate. The residents will recognized the substrate of edible mushrooms on dead trunks of Pasang Tree (Lithocarpus sundaicus), Danglu (Engelhardia spicata), Kemlandingan (Albizia Montana), Casuarina (Casuarina junghuhniana) and acacia (Acacia decurens). Residents who do not have the ability to distinguish and searching were consumer, get the edible mushrooms by buying from the searcher or distributor in packs. The characteristics of an edible mushrooms are a discrete soft flesh of the fruit body, dark color, no ring on the stipe, the presence of insects (e.g. moths) in the lamellae) and the type of mushroom substrate. There were also assisted growths of wild mushrooms by the residents’ raw chop the dead tree trunk as a substrate deliberately. Respondents who knew the time of harvesting edible mushroom were 58.3%. Pasang mushrooms (natural & assisted growth) can be found throughout the rainy season, with an interval of 12 days for subsequent retrieval. While Barat and Kuping mushrooms are found throughout the rainy season, but randomly distributed in the forest. There were unwritten but spoken hereditary local wisdom for the sustainable of the edible mushrooms in Tengger. Only Tenggerese allowed collecting and selling the edible mushrooms in Tengger area to the Tenggerese themselves and tourist (local or
Micromorpholgical studies on senescence process of hyphae in cultivated Auricularia auricula-judae
木耳菌丝老化过程的微形态学研究

YUAN Li,XU Xiu-Hong,
袁立
,许修宏

菌物学报 , 2011,
Abstract: Process of senescence in hyphae of cultivated Auricularia auricula-judae was observed microstructurally and ultrastructurally. In 30d after inoculation, the hyphae were uniform and well-knit; the cell wall was smooth. Under transmission electron microscope, the cells were intact, with abundant inclusions and all organelles were normal. In 60d part swelling hyphae were observed and the color of hyphae were darker. Under transmission electron microscope, the cell wall was unconsolidated, and the mitochondria and vacuole were swollen; nuclei were irregularly swollen, and nucleoli disappeared, and then granules and vesicles increased. Meanwhile, a few electron-dense granules appeared, which indicated the senescence of hyphae. In 90d membrane of part of the nuclei was broken, and a great amount of lipid granules, vesicles and electron-dense granules were observed. The cell wall was futher loose. In 120d lots of hyphae became fractured and the color was much darker. Under transmission electron microscope, collapsion of cell wall and disintegration of cytomembrane system were observed, and part of cell organelles, such as mitochondria, disappeared. In 150d most of hyphae were completely fractured and lost their shapes, and the cytomembrane and cell inclusions were almost disappeared, but part of collapsed cell walls were observed. It was indicated that the senescence process of Auricularia auricula-judae hyphae was irreversible, which developed from individual to whole cells gradually.
STUDIES ON INTERSPECIFIC PROTOPLAST FUSION BETWEEN AUXOTROPHIC MUTANTS OF AURICULARIA AURICULA AND AURICULARIA FUSCOSUCINEA
光木耳和琥珀木耳种间营养缺陷型原生质体融合研究

HAN XIN-CAI,
韩新才
,杨新美

菌物学报 , 1991,
Abstract: 利用60Co-r射线辐照木耳担孢子,获得了9株营养缺陷型突变体。采用光木耳Au-ricularia auricula (L.Ex Hook.)Underw]组氨酸缺陷型菌株 Aa-rH-9和琥珀木耳Auricularia fuscosucinea (Mont.)Farlow] 腺嘌呤缺陷型菌株Af-rH-1进行种间原生质体融合实验,根据营养互补原理,在基础培养基(MM)上检出融合子,获得了稳定的融合子,融合子频率为3.34×10-4—3.76×10-4。初步遗传分析表明:融合子细胞核为单核,是单核异核体,融合子氨基酸含量、酯酶同功酶酶谱均与双亲不同。
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