oalib
Search Results: 1 - 10 of 100 matches for " "
All listed articles are free for downloading (OA Articles)
Page 1 /100
Display every page Item
Cloning and Expression Analysis of RrMYB113 Gene Related to Anthocyanin Biosynthesis in Rosa rugose  [PDF]
Kai Zou, Yang Wang, Mingyuan Zhao, Lanyong Zhao, Zongda Xu
American Journal of Plant Sciences (AJPS) , 2018, DOI: 10.4236/ajps.2018.94055
Abstract: Anthocyanin is one of water-soluble natural pigments widely existing in flowers, fruits, stems, leaves and seeds of plants, and it is the major factor conferring pink or red to the petals of Rosa rugose. MYB TFs play an important role in the anthocyanin synthesis in plants. This work aimed to clone the MYB gene related to anthocyanin synthesis in the petals of Rosa rugose, and explore the relationship between them to lay a good foundation for gene engineering improvement of R. rugose. Based on the transcriptional data, a full-length cDNA sequence of MYB Gene, RrMYB113 (GenBank accession Nos MG720012), was cloned at the first time from the petals of Rosa rugose “Zi zhi” with RT-PCR and RACE methods. The full-length cDNA is 885 bp with an open reading frame of 654 bp, encoding 216 amino acids. The derived RrMYB113 protein has a molecular weight of 25,297.64 Da, a calculated pI of 9.61, a R2R3-MYB domain and bHLH binding domain, and it also has the signature motifs ((A/S/G)NDV and KPRPR(T/S)), thus belonging to Sg6 R2R3-MYB subfamily. In the secondary structure of RrMYB113 protein, there is 37.04% α-helix, 39.81% random coil, 14.81% extended peptide chain, and 8.33% β-corner. There is no transmembrane domain and no signal peptide cleavage site, seventeen Ser phosphorylation sites, fifteen Thr phosphorylation sites, four Tyr phosphorylation sites, and no O-glycosylation sites. The expression of RrMYB113 increased with the color deepening in petals, and it expressed at a higher level in petals than in other tissues of R. rugose “Zi zhi”. These results are meaningful to reveal that RrMYB113 might be an important regulator in anthocyanin biosynthesis and coloration in the petals of R. rugose.
A Study on the Aseptic Germination Method for Rosa rugose Seeds  [PDF]
Changli Fu, Shutang Xing, Dandan Zhao, Dekui Zang, Yuyuan Wei, Lanyong Zhao, Xiaoyan Yu
Agricultural Sciences (AS) , 2017, DOI: 10.4236/as.2017.85032
Abstract: In order to find a method to break dormancy of Rosa rugose seeds and interspecific hybrid seeds between Rosa rugose and Rosa hybrid quickly, and accelerate the breeding process of interspecific hybridization between Rosa rugose and Rosa hybrid, the influence of concentrated acid, seed maturity, GA3 (gibberellin) and low temperature (4°C) on seed germination of Rosa rugose from Muping was researched under aseptic condition. The results showed that aseptic germination can significantly shorten the germination time of Rosa rugose seeds and raise its germination ratio. Before inoculation, concentrated acid treatment greatly increased the germination rate and reduced the contamination rate of the seeds. The higher the degree of maturity of seeds is,
Cloning and Bioinformatics Analysis of Rosa rugose S Locus F-Box Gene (RrSLF)  [PDF]
Yuyuan Wei, Kang Li, Shutang Xing, Dandan Zhao, Changli Fu, Lanyong Zhao, Dekui Zang, Xiaoyan Yu
American Journal of Plant Sciences (AJPS) , 2017, DOI: 10.4236/ajps.2017.87107
Abstract: In order to reveal the phenomenon of R. rugose pollination incompatibility, the full-length cDNA sequence of S Locus F-box Gene was cloned for the first time from the pollen of Rosa rugose “Zilong wochi” with RT-PCR and RACE methods and named as RrSLF. The full-length cDNA is 1236 bp with an open reading frame of 1122 bp, encoding 343 amino acids. The derived protein has a molecular weight of 43.7 kD, a calculated pI of 6.24, an F-box conserved domain at position 343 - 741, and belongs to F-box family. The derived protein is a Hydrophobicity protein secreted into the cytoplasm. There is no transmembrane domain and no signal peptide cleavage site, twenty-one Ser phosphorylation sites, seven Thr phosphorylation sites, seven Tyr phosphorylation sites, two N-glycosylation sites, and no O-glycosylation sites. There are 22.25% α-helixes, 31.37% random coil, 32.17% extended peptide chain, and 14.21% β-corner structure. This protein and the SFB/SLF
Cloning and Expression of One Anthocyanin-Related R2R3-MYB Gene in Rosa rugosa  [PDF]
Yang Wang, Xiaoming Sui, Mingyuan Zhao, Xu Han, Lanyong Zhao, Zongda Xu
American Journal of Plant Sciences (AJPS) , 2018, DOI: 10.4236/ajps.2018.910147
Abstract: Based on the transcriptome of Rosa rugosa, one anthocyanin-promoting R2R3-MYB gene, RrMYB10.1 (Accession Nos:MH717244), was cloned from the petals of Rosa rugosa ‘Zizhi’. Sequence analysis results showed that RrMYB10.1 had a full length opening reading frame of 747bp, encoding 249 amino acids. Sequence analysis revealed that RrMYB10.1 contained the conserved R2R3-MYB domain, two atypical anthocyanin-promoting motifs and a conserved amino acid signature for the interaction with bHLH protein. The results of phylogenic tree revealed that RrMYB10.1 showed high homology with other anthocyanin-promoting proteins in Rosacea, and sharing the highest identity (98.39%) with RhMYB10. RT-PCR results showed that RrMYB10.1 was mainly expressed in petals among various tissues and expressed significantly higher in petals in bud stage than in opening period. To sum up, these results showed that RrMYN10.1 may play a key role in regulating anthocyanin concentration, thus providing a certain foundation on regulating flower color formation in Rosa rugosa.
Cloning and Expression Analysis of RrGT2 Gene Related to Anthocyanin Biosynthesis in Rosa rugosa  [PDF]
Xiaoming Sui, Yang Wang, Mingyuan Zhao, Xu Han, Lanyong Zhao, Zongda Xu
American Journal of Plant Sciences (AJPS) , 2018, DOI: 10.4236/ajps.2018.910146
Abstract: At present, the research about flower color of Rosa rugosa is a very inno-vative and practical study. Glycosylation modification fulfills an important role in increasing the stability and solubility of anthocyanin in plants. In this study, based on the transcriptional database of R. rugosa, a gene with full length cDNA of 1422bp, encoding 473 amino acids, designated as RrGT2, were isolated from flowers of R. rugosa ‘Zizhi’ and then functionally characterized. According to online software prediction, the molecular formula of the protein encoded by the RrGT2 gene is C2334H3628N602O711S18, the relative molecular mass is 52,075.17 Da, and the theoretical isoelectric point is pI = 4.76. The result of the RrGT2 protein 3D model construction showed that it had the highest homology with the UDP-glycosyltransferase 74F2 protein model in the database (39.53%). Sequence alignments with the NCBI database showed that the RrGT2 protein is a member of the GTB superfamily. Homology analysis revealed that the coding regions of RrGT2 was highly specific among different species, but still had typical conserved amino acid residues called PSPG that are crucial for RrGT2 enzyme activity. RrGT2 transcripts were detected in five flowering stages and seven tissues of R. rugosa ‘Zizhi’, R. rugosa ‘Fenzizhi’ and R. rugosa ‘Baizizhi’, and their expression patterns corresponded with the accumulation of antho-cyanins. Therefore, we speculated that glycosylation of RrGT2 plays a crucial role in anthocyanin biosynthesis in R. rugosa.
Cloning and Expression of Anthocyanin Biosynthesis Related Gene RrMYB6 in Rosa rugosa  [PDF]
Kai Zou, Yang Wang, Mingyuan Zhao, Lanyong Zhao, Zongda Xu
Agricultural Sciences (AS) , 2018, DOI: 10.4236/as.2018.93026
Abstract: R2R3-MYB transcription factor plays an important role in plant anthocyanin synthesis. Based on the transcriptional database of Rosa rugosa, one MYB transcription factor related to floral color, RrMYB6, was cloned. By using bioinformatics analysis method, cloning MYB gene and analyzing its function in anthocyanin biosynthesis regulation, we hope to lay a solid foundation for new color variety breeding of R. rugosa. Using the R. rugosa “Zi zhi” as the material, we obtained the total length of cDNA of RrMYB6 by RT-PCR and RACE. By analyzing its bioinformatics, we found that the formula of the protein was C1491H2368N452O470S17, molecular weight was 34690.97 Da, the theoretical pI was 8.74. In addition, it belonged to unstable protein with an unstable index at 50.59, and it was also a hydrophilic protein with the total average hydrophobic index at -0.847. In the secondary structure of RrMYB6 protein, the Alpha helix accounted for 32.35%, random coil was 47.39%, extended strand was 11.11%, and beta turn was 9.15%. The sequence analysis showed that RrMYB6 had a typical R2R3-MYB domain and bHLH binding domain, and it also had an N1, C1, C2 inhibitory motif, belonging to the Sg4 subfamily MYB protein. What’s more, evolutionary analysis indicated that the RrMYB6 protein was closely related with the MYB protein in Rosacea family, while it was far from those in other families. The expression analysis showed that RrMYB6 protein decreased with the color of petals deeping, and its expression was the lowest in the petals while the highest in stamens. According to the above results, it was speculated that RrMYB6 was involved in regulating the anthocyanin synthesis of R. rugosa, which belonged to negative regulatory mechanism.
Overexpression of the Rosa rugosa RrGT1 Gene Induces Anthocyanin Accumulation in Tobacco  [PDF]
Xiaoming Sui*, Mingyuan Zhao*, Xu Han, Lanyong Zhao#, Zongda Xu#
Natural Science (NS) , 2018, DOI: 10.4236/ns.2018.1010038
Abstract: Rosa rugosa has always been an important plant in landscape application, and the improvements and innovations about its flower color are particularly important. Glycosylation modification fulfills an important role in increasing the stability and solubility of anthocyanin in plants. In this study, based on the transcriptional database of R. rugosa, a gene with full length cDNA of 1161 bp, encoding 386 amino acids, designated as RrGT1, were isolated from flowers of R. rugosa Zizhi and then functionally characterized. Sequence alignments with the NCBI database show that the RrGT1 protein is a member of the GTB superfamily and has typical conserved amino acid residues called PSPG that are crucial for RrGT1 enzyme activity. RrGT1 transcripts were detected in five flowering stages and seven tissues of R. rugosa Zizhi and their expression patterns corresponded with the accumulation of anthocyanins. Additionally, the in vivo function of RrGT1 was investigated via its overexpression in tobacco. Transgenic tobacco plants expressing RrGT1 induced anthocyanin accumulation in flowers, indicating that RrGT1 could encode a functional glycosyltransferase (GT) protein for anthocyanin biosynthesis and could function in other species. Therefore, we speculated that glycosylation of RrGT1 played a crucial role in anthocyanin biosynthesis in R. rugosa.
Cloning and Expression Analysis of RrGT1 Gene Related to Anthocyanin Biosynthesis in Rosa rugosa  [PDF]
Xiaoming Sui, Pengyuan Zhang, Yu Wang, Mingyuan Zhao, Xu Han, Lanyong Zhao, Zongda Xu
Agricultural Sciences (AS) , 2018, DOI: 10.4236/as.2018.98075
Abstract: Glycosylation modification fulfills an important role in increasing the stability and solubility of anthocyanin in plants. In this study, based on the transcriptional database of R. rugosa, a gene with full length cDNA of 1161 bp, encoding 386 amino acids, designated as RrGT1, was isolated from flowers of R. rugosa ‘Zizhi’ and then functionally characterized. According to online software prediction, the molecular formula of the protein encoded by the RrGT1 gene is C1879H2964N494O556S14, the relative molecular mass is 41,820.02 Da, and the theoretical isoelectric point is pI = 5.03. The result of the RrGT1 protein 3D model construction showed that it had the highest homology with the UDP-glucose: anthocyanidin 3-O-glucosyltransferase protein model in the database (47.01%). Sequence alignments with the NCBI database showed that the RrGT1 protein is a member of the GTB superfamily. Homology analysis revealed that the coding regions of RrGT1 was highly specific among different species, but still had typical conserved amino acid residues called PSPG that are crucial for RrGT1 enzyme activity. RrGT1 transcripts were detected in five flowering stages and seven tissues of R. rugosa ‘Zizhi’, R. rugosa ‘Fenzizhi’ and R. rugosa ‘Baizizhi’, and their expression patterns corresponded with the accumulation of anthocyanins. Therefore, we speculated that glycosylation of RrGT1 plays a crucial role in anthocyanin biosynthesis in R. rugosa.
Focus Structure in Persian Interrogative Sentences: An RRG Analysis  [cached]
Vali Rezai,Mozhgan Hooshmand
English Language Teaching , 2012, DOI: 10.5539/elt.v5n8p130
Abstract: The studies regarding information structure and its distribution in sentences are traced back to works of Prague School linguists such as Mathesius in 1920s. Recently, the issue of information structure has been dealt with by functionalists. In Role and Reference Grammar (RRG), information structure constitutes one of the main components of syntax. In this theory, information structure is mainly based on the Lambrechtiyan information structure which regards the formal structure of sentences as highly related to the discourse-pragmatic functions. Information structure investigates the way information is structured in clauses and sentences. This paper aims at dealing with information structure in interrogative sentences according to RRG. At first a brief introduction to works on information structure and RRG is presented. Then the theory is applied briefly to Persian language declaratives and dominantly to interrogatives.
ROSA Analyser: An automatized approach to analyse processes of ROSA
Raúl Pardo,Fernando L. Pelayo
Electronic Proceedings in Theoretical Computer Science , 2012, DOI: 10.4204/eptcs.86.4
Abstract: In this work we present the first version of ROSA Analyser, a tool designed to get closer to a fully automatic process of analysing the behaviour of a system specified as a process of the Markovian Process Algebra ROSA. In this first development stage, ROSA Analyser is able to generate the Labelled Transition System, according to ROSA Operational Semantics. ROSA Analyser performance starts with the Syntactic Analysis so generating a layered structure, suitable to then, apply the Operational Semantics Transition rules in the easier way. ROSA Analyser is able to recognize some states identities deeper than the Syntactic ones. This is the very first step in the way to reduce the size of the LTS and then to avoid the state explosion problem, so making this task more tractable. For the sake of better illustrating the usefulness of ROSA Analyser, a case study is also provided within this work.
Page 1 /100
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.