Search Results: 1 - 10 of 100 matches for " "
All listed articles are free for downloading (OA Articles)
Page 1 /100
Display every page Item
International Journal of Bioinformatics Research , 2011,
Abstract: Alanine aminotransferase (ALT) is an enzymatic protein involved in catabolism of amino acids. The carbondistribution study on this clinically important protein is carried out here. The study reveals that the carbon content is generallyhigher than the expected values of 31.45%. The alteration in carbon content other than the active site might improve theactivity of this enzymatic protein. Particularly the reduction at the carboxyl end of the sequence is more appropriate. Carbondistribution analysis clearly locates the active site of ALT protein, which is reported here.
Alanine Aminotransferase Decreases with Age: The Rancho Bernardo Study  [PDF]
Mamie H. Dong,Ricki Bettencourt,Elizabeth Barrett-Connor,Rohit Loomba
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0014254
Abstract: Serum alanine aminotransferase (ALT) is a marker of liver injury. The 2005 American Gastroenterology Association Future Trends Committee report states that serum ALT levels remain constant with age. This study examines the association between serum ALT and age in a community-dwelling cohort in the United States.
Clinical characteristics associated with hepatic steatosis on ultrasonography in patients with elevated alanine aminotransferase
Narciso-Schiavon, Janaína Luz;Schiavon, Leonardo de Lucca;Carvalho-Filho, Roberto José de;Hayashida, Débora Yumi;Wang, Jenny Hue Jiuan;Souza, Tatiana Santana;Emori, Christini Takemi;Ferraz, Maria Lucia Gomes;Silva, Antonio Eduardo Benedito;
Sao Paulo Medical Journal , 2010, DOI: 10.1590/S1516-31802010000600006
Abstract: context and objective: the main causes of hepatic steatosis (hs) are alcoholic liver disease and nonalcoholic fatty liver disease (nafld). although liver biopsy is the gold standard for nafld diagnosis, the finding of abnormal aminotransferases in abstinent individuals, without known liver disease, suggests the diagnosis of nafld in 80-90% of the cases. identification of clinical factors associated with hs on abdominal ultrasound may enable diagnoses of fatty liver non-invasively and cost-effectively. the aim here was to identify clinical variables associated with hs in individuals with elevated alanine aminotransferase (alt) levels. design and setting: cross-sectional study in a single tertiary care center. methods: individuals with elevated alt, serologically negative for hepatitis b and c, were evaluated by reviewing medical files. patients who did not undergo abdominal ultrasonography were excluded. results: among 94 individuals included, 40% presented hs on ultrasonography. compared with individuals without hs, those with fatty liver were older (p = 0.043), with higher body mass index (bmi) (p = 0.003), diabetes prevalence (p = 0.024), fasting glucose levels (p = 0.001) and triglycerides (p = 0.003). multivariate analysis showed that bmi (odds ratio, or = 1.186; 95% confidence interval, ci: 1.049-1.341; p = 0.006) and diabetes mellitus (or = 12.721; 95% ci: 1.380-117.247; p = 0.025) were independently associated with hs. conclusions: simple clinical findings such as history of diabetes and high bmi may predict the presence of hs on ultrasonography in individuals with elevated alt and negative serological tests for hepatitis.
Alanine-aminotransferase: an early marker for insulin resistance?
Salazar,Martín R.; Carbajal,Horacio A.; Curciarello,José O.; Aizpurua,Marcelo; Adrover,Raúl E.; Riondet,Beatriz;
Medicina (Buenos Aires) , 2007,
Abstract: in a population-based sample, after excluding alcohol consumption, hepatotoxic drugs and hepatitis band c infected, we investigated if alanine-aminotransferase (alt) was associated with metabolic syndrome and insulin resistance, and if this association was caused by non-alcoholic fatty liver disease (nafld). the sample (432 female and 119 male) was divided into two alt thresholds corresponding to the 50th and 75th percentiles (p) (female ≤ 15 and ≤ 19 u/l; male ≤ 17 and ≤ 23 u/l, respectively). blood pressure, body mass index, waist circumference, cholesterol, hdl cholesterol (hdlc), triglyceride (tg), tg/hdlc ratio, glycemia and homeostasis model assessment of insulin resistance (homa-ir) were compared between those above and below each alt threshold. female placed above the 50th p of alt had higher levels of tg/hdlc ratio (p=0.029), glycemia (p=0.028), and homeostasis model assessment of insulin resistance, (p=0.045), and above the 75th p had higher sbp (p=0.036), dbp (p=0.018), tg (p=0.024), tg/hdlc ratio (p=0.028), glycemia (p=0.004) and homa-ir (p=0.0014). male placed above the 50th p of alt had higher bmi (p=0.017) and tg/hdlc ratio (p=0.048), and above the 75th p had lower values of hdlc (p=0.042). only 16.5% of women and 14.5% of men, above the 75th p of alt, showed an increase in liver brightness in the echography. this work shows in woman an early association of alt with tg/hdlc ratio and homa-ir. since the last two are independent predictors of cardiovascular risk, attention should be drawn to alt values near the upper limit of the normal range even in the absence of nafld and obesity.
Aspartate Aminotransferase (AST/GOT) and Alanine Aminotransferase (ALT/GPT) Detection Techniques  [PDF]
Xing-Jiu Huang,Yang-Kyu Choi,Hyung-Soon Im,Oktay Yarimaga,Euisik Yoon,Hak-Sung Kim
Sensors , 2006, DOI: 10.3390/s6070756
Abstract: The levels of aspartate aminotransferase (AST/GOT) and alanineaminotransferase (ALT/GPT) in serum can help people diagnose body tissues especially theheart and the liver are injured or not. This article provides a comprehensive review ofresearch activities that concentrate on AST/GOT and ALT/GPT detection techniques due totheir clinical importance. The detection techniques include colorimetric, spectrophotometric,chemiluminescence, chromatography, fluorescence and UV absorbance, radiochemical, andelectrochemical techniques. We devote the most attention on experimental principle. Insome methods a few representative devices and important conclusions are presented.
Histological and Clinical Characteristics of Patients with Chronic Hepatitis C and Persistently Normal Alanine Aminotransferase Levels  [PDF]
Bakht Roshan,Grace Guzman
Hepatitis Research and Treatment , 2014, DOI: 10.1155/2014/760943
Abstract: Patients with chronic hepatitis C virus (HCV) infection and persistently normal alanine aminotransferase (PNALT) are generally described to have mild liver disease. The aim of this study was to compare clinical and histological features in HCV-infected patients with PNALT and elevated ALT. Patients presenting to the University of Illinois Medical Center, Chicago, who had biopsy proven HCV, an ALT measurement at the time of liver biopsy, at least one additional ALT measurement over the next 12 months, and liver biopsy slides available for review were identified. PNALT was defined as ALT ≤ 30 on at least 2 different occasions over 12 months. Of 1200 patients with HCV, 243 met the study criteria. 13% (32/243) of patients had PNALT while 87% (211/243) had elevated ALT. Significantly more patients with PNALT had advanced fibrosis (F3 and F4) compared to those with elevated ALT ( ). There was no significant difference in the histology activity index score as well as mean inflammatory score between the two groups. In conclusion, in a well-characterized cohort of patients at a tertiary medical center, PNALT did not distinguish patients with mild liver disease. 1. Introduction Hepatitis C virus (HCV) infection is reported to have a prevalence of approximately 3% worldwide [1]. Almost 80% of those infected go on to develop chronic infection. Majority of patients with chronic HCV have a mild, asymptomatic elevation in serum transaminase levels with no significant clinical symptoms. Around 25% of patients with chronic HCV have persistently normal alanine aminotransferase (PNALT) [2]. Definition of normal alanine aminotransferase (ALT) has changed over time and reference range for normal ALT differs based on different laboratory cutoffs. Prati et al. [3] in 2002 suggested new cutoffs with 30?U/L (international unit) for men and 19?U/L for women compared to 40?U/L and 30?U/L for men and women, respectively. This resulted in improved sensitivity but decreased specificity. Similarly, definition of PNALT differs widely. A 2009 American Association for the Study of Liver Disease (AASLD) practice guideline suggested an ALT value of 40?U/L on 2-3 different occasions separated by at least a month over a period of 6 months [4]. Others have used 3 different ALT levels equal to or below upper limit of normal (ULN) separated by at least 1 month and sometimes over a period of 18 months [5]. Thus, there is no consensus on a universal definition of PNALT. It was generally thought that people with PNALT have a mild liver disease and the degree of liver fibrosis is minimal [6–14].
Effects of Dietary Vitamin E and Selenium on Aspartate Aminotransferase and Alanine Aminotransferase Activities in Rats Treated with High Doses of Glucocorticoid
Mine Erisir,Ebru Beytut,Fatih Mehmet Kandemir,Fulya Benzer
Journal of Animal and Veterinary Advances , 2012,
Abstract: The effects of dietary intake of vitamin E and selenium on aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in rats treated with high doses of prednisolone were investigated. Rats were divided into 5 groups. Groups 3, 4 and 5 received a daily supplement in their drinking water of 20 mg vitamin E, 0.3 mg Se and a combination of vitamin E and Se, respectively, for 30 days. For 3 days subsequently, the control group (group 1) was given a placebo and the remaining 4 groups were injected intramuscularly with 100 mg kg-1 body weight prednisolone. After the last administration of prednisolone, 10 rats from each group were killed at 4, 8, 12, 24 and 48 h and the activities of aspartate aminotransferase and alanine aminotransferase enzymes in their tissues were measured. In the group, treated with prednisolone alone, AST activity in the liver was not affected, the ALT activity was significantly decreased at 12 h only. AST and ALT activities in the kidneys were significantly decreased by prednisolone at all time periods. AST activity in the heart also decreased in the prednisolone group between 4 and 24 h, significantly at 12 h. Significant decreases were found at 4, 8 and 12 h in the heart ALT activity of the prednisolone administered group. AST activity in the liver, kidneys and heart was lower in vitamin E and Se supplemented groups than control and prednisolone groups. In the combination group, compared to both the control and prednisolone groups, AST activity in the kidneys and heart was decreased, but increased in liver. Vitamin E and Se alone or in combination had a preventive effect on the decrease of ALT activity in the liver and kidneys caused by prednisolone. ALT activity in the heart tissue of the vitamin E supplemented group was found to be increased at all time periods, however, it reduced in the Se and combination groups compared to the prednisolone group. Therefore, the present study demonstrates that vitamin E and Se alone or in combination may prevent the decrease in ALT activity in the liver and kidneys caused by high doses of prednisolone.
Serum alanine aminotransferase levels, hematocrit rate and body weight correlations before and after hemodialysis session
Lopes, Edmundo Pessoa;Sette, Luis Henrique B. C.;Sette, Jorge Bezerra C.;Luna, Carlos F.;Andrade, Amaro M.;Moraes, Maviael;Sette, Paulo C. A.;Menezes, Roberto;Cavalcanti, Rui L.;Concei??o, Sergio C.;
Clinics , 2009, DOI: 10.1590/S1807-59322009001000002
Abstract: purpose: to evaluate alanine aminotransferase levels before and after a hemodialysis session and to correlate these values with the hematocrit rate and weight loss during hemodialysis. patients and methods: the serum alanine aminotransferase levels, hematocrit rate and body weight were measured and correlated before and after a single hemodialysis session for 146 patients with chronic renal failure. an receiver operating characteristic (roc) curve for the serum alanine aminotransferase levels collected before and after hemodialysis was plotted to identify hepatitis c virus-infected patients. results: the mean weight loss of the 146 patients during hemodialysis was 5.3% (p < 0.001). the mean alanine aminotransferase levels before and after hemodialysis were 18.8 and 23.9 iu/, respectively, denoting a significant 28.1% increase. an equally significant increase of 16.4% in the hematocrit rate also occurred after hemodialysis. the weight loss was inversely correlated with the rise in both the alanine aminotransferase level (r = 0.3; p < 0.001) and hematocrit rate (r = 0.5; p < 0.001). a direct correlation was found between the rise in alanine aminotransferase levels and the hematocrit during the hemodialysis session (r = 0.4; p < 0.001). based on the roc curve, the upper limit of the normal alanine aminotransferase level should be reduced by 40% relative to the upper limit of normal if the blood samples are collected before the hemodialysis session or by 60% if blood samples are collected after the session. conclusion: in the present study, significant elevations in the serum alanine aminotransferase levels and hematocrit rates occurred in parallel to a reduction in body weight after the hemodialysis session. these findings suggest that one of the factors for low alanine aminotransferase levels prior to hemodialysis could be hemodilution in patients with chronic renal failure.
Cytokeratin 18, Alanine Aminotransferase, Platelets and Triglycerides Predict the Presence of Nonalcoholic Steatohepatitis  [PDF]
Wei Cao, Caiyan Zhao, Chuan Shen, Yadong Wang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0082092
Abstract: Background Nonalcoholic fatty liver disease (NAFLD) is one of the critical public health problems in China. The full spectrum of the disease ranges from simple steatosis and nonalcoholic steatohepatitis (NASH) to cirrhosis and hepatocellular carcinoma(HCC). The infiltration of inflammatory cells characterizes NASH. This characteristic contributes to the progression of hepatitis, fibrosis, cirrhosis, and HCC. Therefore, distinguishing NASH from NAFLD is crucial. Objective and Methods Ninety-five patients with NAFLD, 44 with NASH, and 51 with non-NASH were included in the study to develop a new scoring system for differentiating NASH from NAFLD. Data on clinical and biological characteristics, as well as blood information, were obtained. Cytokeratin-18 (CK-18) fragments levels were measured using an enzyme-linked immunosorbant assay. Results Several indexes show significant differences between the two groups, which include body mass index (BMI), waist-on-hip ratio (WHR), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), γ-glutamyl transpeptidase (γ-GT), platelets, uric acid (UA), hs-C-reactive protein (hs-CRP), triglycerides (TG), albumin (ALB), and CK-18 fragments (all P < 0.05). The CK-18 fragment levels showed a significant positive correlation with steatosis severity, ballooning, lobular inflammation, and fibrosis stage (all P < 0.05). Therefore, a new model that combines ALT, platelets, CK-18 fragments, and TG was established by logistic regression among NAFLD patients. The AUROC curve in predicting NASH was 0.920 (95% CI: 0.866 - 0.974, cutoff value = 0.361, sensitivity = 89%, specificity = 86%, positive predictive value = 89%, negative predictive value = 89%). Conclusion The novel scoring system may be considered as a useful model in predicting the presence of NASH in NAFLD patients.
Activities of Aspartate Aminotransferase, Alanine Aminotransferase, Gamma-Glutamyltransferase, Alkaline Phosphatase in Plasma of Postpartum Holstein Cows
Ping Liu,BaoXiang He,XianLing Yang,XiaoLu Hou,HaiYang Zhao,YinHua Han,Pei Nie,HuiFang Deng,Long Cheng
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2012.1270.1274
Abstract: Depressed appetite and reduced Dry Matter Intake (DMI) and feeding energy-dense diets for a long time around parturition of cows may lead to excessive lipid mobilization which causes the liver damage. This study was meant to determine the effects of postpartun enzymes metabolic status in Holstein cows. In this study, blood samples were during the whole experimental period, obtained from the jugular venepuncture from each animal on 1 week prepartum (week 1), days delivery (week 0) and 1st 9 weeks postpartum (week 1-9). They were analyzed for examining Aspartate aminotransferase (AST), Alanine Aminotransferase (ALT), Gamma-Glutamyltransferase (GGT), Alkaline Phosphatase (ALP) activity. The resultes showed a higher activity of AST which was determined in the 1-3 weeks than other s. ALT activity indicated a statistically significant increase from the 5-7 weeks of lactation and activity in the 7th week postpartum periods significally reached to the peak. GGT activity in the antepartum 1 week until delivery day was significally lower in comparison with the first to reach the 9th weeks postpartum. ALP activity in the delivery day and 6-8 weeks significant increased in process. Therefore, the AST, ALT, GGT and ALP of enzyme activity which could be used significantly change in the blood plasma of Holstein.
Page 1 /100
Display every page Item

Copyright © 2008-2017 Open Access Library. All rights reserved.