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转BnTR1基因的烟草对多种逆境胁迫的抗性研究
The analysis of BnTR1 enhances a variety of resistance in Nicotiana benthamiana
 [PDF]

曹昊昊,王中浩,范智勇
- , 2016,
Abstract: 全球气候变化导致植物频繁遭遇干旱和盐胁迫,研究植物抗旱耐盐并培育抗旱耐盐植物新材料已经成为植物研究领域的热点问题.来源于油菜的BnTR1是一个定位于膜上的E3连接酶,该基因表现出热的诱导性并赋予植物耐热特定性.为了研究BnTR1的其他抗逆能力,本实验将该基因转化到了本生烟中,并研究了转基因烟草对干旱和盐胁迫下的生长发育变化,发现与非转基因对照相比,转基因烟草对甘露醇和PEG模拟干旱表现出较强的抗性,尤其是PEG胁迫时,三个转基因株系在生物量的积累和整体长势方面都优于对照.干旱处理20d后复水并培养14d后,对照烟草全部枯死,但是所有转基因烟草都恢复生长直至开花结果.这些结果都说明BnTR1赋予转基因烟草对干旱的抗旱特性.当把烟草叶片圆片浸泡在系列盐水中,盐浓度超过600mM时,对照烟草圆片受损白化,而转基因烟草圆片能保持较大面积的绿色,说明在盐胁迫时转基因烟草更能保持叶绿体和叶绿素的稳定,也说明转基因烟草对盐胁迫的抗性的增强.在热激处理后,热激蛋白基因HSP90和HSP70在所有烟草中的表达量都提高了,增加的量在对照和转基因株系中并无差异,而正常条件下不表达的HSF30 和sHSP17.6 都表达了,HSF30增加了50%,sHSP17.6增加了30%.这些结果说明了转BnTR1基因增强了转基因烟草对多种逆境胁迫的抗性,为进一步研究BnTR1基因的功能奠定了基础,也为其应用提供了实验证据.
Abstract: Owing to the global climate change, frequent drought and salt stress have happened to most plants. As a result, it has become a hot issue in agricultural field to research in tolerance mechanisms of plants and to cultivate new plant materials that can tolerate salt and drought. Derived from rape, BnTR1 is an E3 ligase located in the membrane, and its expression can be induced by heat stress,and then gives the heat resistance to plants. BnTR1 was transformed into Nicotiana benthamiana to study the growth of transgenic Nicotiana benthamiana under drought and salt stress.What’s more, compared with the control, this transgenic plant shows a better resistance to mannitol and PEG, especially in growth status and accumulation of biomass under PEG stress. After 20 days under drought treatment, plants were rewatered and then cultivated for 2 weeks, and all transgenic Nicotiana benthamiana were recovered from wilting, and all wild type Nicotiana benthamiana died. These results indicated that Nicotiana benthamiana can be resistant to drought with BnTR1. When leaves were treated with hydrosaline solution, transgenic Nicotiana benthamiana could maintain green even as salt concentration was above 600mM, while control plants became albino. This result suggested that chloroplast and chlorophyll in transgenic Nicotiana benthamiana can be more stable under salt stress, and transgenic Nicotiana benthamiana can be more resistant to salt stress. After heat shock, the expression of HSP90 and HSP70 in transgenic Nicotiana benthamiana and controls were all up-regulated, and there were no significant difference in increment between transgenic plants and controls. The expression of HSF30 and sHSP17.6 were also induced which can’t be detected in normal condition, HSF30 increasing 50% and sHSP17.6 increasing 30%. These researches illustrated that Nicotiana benthamiana can survive in many stressful conditions when transformed with BnTR1. This experiment laid a good
过量表达BnTR1的油菜对多种逆境的综合抗性分析
Analysis of comprehensive tolerance to abiotic stresses in Brassica nupus over-expressing BnTR1
 [PDF]

陈彩丽,曹昊昊,樊莉娟,朱旭辉,刘志斌,王健美
- , 2017,
Abstract: 油菜BnTR1是一个定位于质膜上的E3连接酶,前期的研究表明它赋予油菜、水稻和大肠杆菌对热胁迫的耐受性。为了研究过量表达BnTR1的油菜对多种逆境的综合抗逆特性,本研究设计了多种胁迫条件,观察并分析了这些油菜的综合抗逆表现。结果表明与对照材料相比,转基因油菜对双氧水胁迫、盐和甘露醇模拟干旱胁迫表现出较强的抗性,并且在盐胁迫下,转基因油菜的SOD、POD、和CAT三种抗氧化酶活性高于对照材料。这些结果证实过量的BnTR1使得油菜对多种胁迫表现出了较强的抗性,为深入研究BnTR1的功能奠定了基础,也为将这些油菜用作抗逆油菜新品种培育提供了原始材料。
BnTR1 is an E3 ligase localized in the plasma membrane, and the previous studies indicated that it played a key role in heat stress response in Brassica nupus, rice and E. coli. In order to investigate the comprehensive resistance characteristic of B.napus over-expressing BnTR1 lines, this research designed a variety of stress conditions to observe and analysis their phenotype. Results showed that compared with the control, the transgenic plant showed a better resistance to hydrogen peroxide, salt, and mannitol. In addition, the activity of superoxide dismutase(SOD),peroxidas (POD) and catalase(CAT) in transgenic plant was higher than the control under salt stress. These results confirmed that the over expression of BnTR1 enhanced the tolerance of Brassica nupus against a variety of stress, which laid a foundation for further research of the function of BnTR1, also provided original materials for breeding new varieties
甲基莲心碱对血管平滑肌α受体引起45Ca外溢与内流的影响  [PDF]
王永信
中国中药杂志 , 1996,
Abstract: 在血管平滑肌,甲基莲心碱能明显减少苯肾上腺素引起的45Ca内流量,不影响高钾引起的45Ca内流;而硝苯吡啶几乎完全阻断后一种的45Ca内流。甲基莲心碱不抑制45Ca外溢。
若干化学毒物对泥蚶吸收45Ca的影响  [PDF]
蔡福龙,陈英,黄凌毅
华东政法大学学报 , 1991,
Abstract: 本文研究几种化学毒物对泥蚶吸收45Ca的影响,结果表明Cu,Zn,Pd,Cd,Hg等重金属元素的无机化合物对泥蚶的血液、软组织吸收45Ca有抑制作用,其中Cd的抑制作用最强,其次是Cu和Hg.但这些金属元素对泥蚶外壳吸收45Ca的抑制作用不大.三种能量代谢抑制剂对泥蚶吸收45Ca的作用表明45Ca的吸收是主动传输过程.
酸性pH及铝对鲤鱼(Cyprinuscarpio)吸收45Ca的影响  [PDF]
孔繁翔,周风帆,张珍凤,郭丽君
环境化学 , 2015,
Abstract: 本文用放射性核素45Ca作为示踪剂,研究在酸性pH及加铝的条件下,鲤鱼(Cyprinuscarpio)对钙离子的吸收分布情况.结果表明,在pH值为7.10时,45Ca在鲤鱼体内各器官的96h放射仕比度为鳃13886cpm·g-1,骨骼10811cpm·g-1,肝脏3276cpm·g-1·肌肉2865cpm·g-1;在酸性pH(4.30)条件下,鲤鱼对钙离子的吸收和积累受到明显抑制;加铝后,鲤鱼体内各器官45Ca放射性比度与未加铝的对照组相比,下降百分比分别为鳃42.4%.骨骼18.5%,肝脏44.2%并讨论了酸雨危害鱼体钙代谢的可能影响.
低pH值及铝对深鳅吸收45Ca的影响  [PDF]
孔繁翔,周风帆,陈爽
环境科学 , 1996,
Abstract: 为了探讨低PH和铝对水生生物的毒性或联合毒性效应,进一步研究钙缓解作用机理,用放射性核力45Ca作为示踪剂,研究在低pH值及加铝条件下,泥鳅(Misgurnusanguillicaudatus)对钙离子的吸收分布情况。结果表明,生长在pH为7.10时,45Ca在泥鳅体内各器官的96h放射性比度为:皮肤39532Cpm/g,骨骼38116Cpm/g,鳃25495cpm/g,肌肉1651cpm/s;低pH(4.70)条件下,泥鳅体内45Ca含量较正常条件下(对照组PH7.0)下降百分比为:皮肤81.07%,骨骼84.41%,鳃80.11%,肌肉5.88%;在低PH值并加铝时,泥鳅体内备器官45Ca含量与对照组相比,下降百分比分别为:皮肤89.87%.骨骼88.83%,鳃86.17%,肌肉26.47%.并讨论了酸雨危害对鱼体钙代谢的可能影响。
富 宁 八 角 茴 香 最 佳 采 收 期 研 究  [PDF]
李洪潮,张志信,张仕秀
北方园艺 , 2010, DOI: 10.11937/bfyy.201018006
Abstract:
响叶杨(populusadenopoda)种子的形成及其寿命  [PDF]
吴琼美,樊汝汶
南京林业大学学报(自然科学版) , 1984, DOI: 10.3969/j.jssn.1000-2006.1984.01.014
Abstract: <正>用显微技术及软x射线摄影,定位观察响叶杨种子发育过程中的形态变化与胚胎发育的对应关系。根据观察、试验的结果,讨论了环境条件与种子寿命的关系;还测定了响叶杨种子寿命的变化规律及其影响因素,探索延长这种子寿命的方法。
响蜜Lie科的新记录
杨岚
动物学研究 , 1995,
Abstract: 1994年10月15日马中翼等人在云南省泸水县的老窝捕获到1只雄鸟,经中国科学院昆明动物研究所鉴定为黄腰响蜜lie,为我国鸟类科的新记录。标本保存在中国科学院昆明动物研究所鸟类标本室。科的特征体形较小。嘴形似雀,短而粗实,上嘴稍弯曲;尾羽12枚;翅形长而尖,仅具9枚初级飞羽;脚呈对趾型,第2、3趾向前,第1、4趾向后,似啄木鸟。
响蜜lie科的新记录  [PDF]
动物学研究 , 1995,
Abstract: 1994年10月15日马中翼等人在云南省泸水县的老窝捕获到1只雄鸟,经中国科学院昆明动物研究所鉴定为黄腰响蜜lie,为我国鸟类科的新记录。标本保存在中国科学院昆明动物研究所鸟类标本室。科的特征体形较小。嘴形似雀,短而粗实,上嘴稍弯曲;尾羽12枚;翅形长而尖,仅具9枚初级飞羽;脚呈对趾型,第2、3趾向前,第1、4趾向后,似啄木鸟。
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