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Effects of Preheated Treatments on Physicochemical Properties of Resistant Starch Type III from Pullulanase Hydrolysis of High Amylose Rice Starch
J. Pongjanta,A. Utaipattanaceep,O. Naivikul,K. Piyachomkwan
American Journal of Food Technology , 2009,
Abstract: In this study, the effects of preheated treatments on physicochemical properties of resistant starch type III formation by pullulanase hydrolysis of High Amylose Rice Starch (HARS) were investigated. A debranching enzyme (Pullulanase, 8 U g-1 starch at 55°C for 0-48 h) was introduced to modify the amylopectin molecules of 15% (w/w) HARS suspension (32.10%, amylose content) which had been preheated at 95 and 121°C for 30 min. Retrogradation gels of debranched starches with different degrees of hydrolysis (0.14 to 3.10%) were then induced at 4°C for 16 h. Afterward, one cycle of the freeze-thaw process (-10/30°C) was applied to promote syneresis of the retrograded starches. Results show that pullulanase hydrolysis enhanced the degree of syneresis (33.22, 45.27 and 58.91% for non-debranched and debranched starches which had been preheated at 95 and 121°C for 48 h, respectively). The debranched starches with higher degree of hydrolysis provided products with higher resistant starch contents. The resistant starch content increased quadrupled with debranching and the freeze-thaw process (4.07 to 10.68% and 5.12 to 19.32% for 0 to 48 h pullulanase hydrolysis of HARS preheated at 95 and 121°C, respectively). Results had shown that after debranching and retrogradation, the HARS molecules had rearranged and changed their crystal pattern from A to V-type pattern, as revealed by X-ray diffraction analysis. In vitro starch hydrolysis index of the RS III samples from 0 to 48 h of pullulanase hydrolysis of the HARS which had been preheated at 95 and 121°C were reduced from 71.591 to 41.69% and 68.66 to 26.83%, respectively.
PULLULANASE: A POTENTIAL ENZYME FOR INDUSTRIAL APPLICATION  [cached]
Malviya S. N.,Ramdas Malakar,Archana Tiwari
International Journal of Biomedical Research , 2011, DOI: 10.7439/ijbr.v1i2.53
Abstract: Pullulanase is one of the most important enzymes in starch processing. This enzyme is used on a large scale in glucose and maltose syrup industries. In recant times, the agricultural wastes rich in polysaccharides can be processed to useful from the sucrose and glucose instant of dumping. Pullulanase is a very potent enzyme for degradation of starch to glucose or maltose. Pullulanase hydrolyses α-1, 6-glycosidic linkage of branched chain and α-1, 4-glycosidic linkage and α-1, 6-glycosidic linkage of polysaccharides. It is also known as de-branching enzyme. Pullulanase has been used in some industries like glucose and maltose syrup production, baking and cyclodextrin production recently. In future pullulanase enzyme can be widely used in industries if improve we the stability and activity of this enzyme upon searching novel extremophile microorganism. The number of applications will increase manifolds and with the availability of thermostable enzyme a number of new possibilities for industrial processes shall emerge.
PULLULANASE: A POTENTIAL ENZYME FOR INDUSTRIAL APPLICATION
Malviya S. N.,Ramdas Malakar,Archana Tiwari
International Journal of Biomedical Research , 2013, DOI: 10.7439/ijbr.v1i2.53
Abstract: Pullulanase is one of the most important enzymes in starch processing. This enzyme is used on a large scale in glucose and maltose syrup industries. In recant times, the agricultural wastes rich in polysaccharides can be processed to useful from the sucrose and glucose instant of dumping. Pullulanase is a very potent enzyme for degradation of starch to glucose or maltose. Pullulanase hydrolyses ±-1, 6-glycosidic linkage of branched chain and ±-1, 4-glycosidic linkage and ±-1, 6-glycosidic linkage of polysaccharides. It is also known as de-branching enzyme. Pullulanase has been used in some industries like glucose and maltose syrup production, baking and cyclodextrin production recently. In future pullulanase enzyme can be widely used in industries if improve we the stability and activity of this enzyme upon searching novel extremophile microorganism. The number of applications will increase manifolds and with the availability of thermostable enzyme a number of new possibilities for industrial processes shall emerge.
Effects of autoclaving and pullulanase debranching on the resistant starch yield of normal maize starch  [PDF]
MARIJA S. MILA?INOVI?,MILICA M. RADOSAVLJEVI?,LJUBICA P. DOKI?
Journal of the Serbian Chemical Society , 2010,
Abstract: In this study, resistant starch (RS), type 3, was prepared by the autoclaving and debranching of normal maize starch isolated from a selected ZP genotype. The objectives of this study were to optimize both starch autoclaving and debranching with pullulanase (PromozymeBrewQ) for the production of RS. Autoclaving at 120 °C (30 min) increased the RS content of all samples, whereas freezing at –20 °C did not have an obvious effect on the RS contents. The highest RS yield in the autoclaved starch samples was 7.0 % after three autoclaving–cooling cycles. After pullulanase debranching at 50 °C and retrogradation at 4 °C, the RS yields ranged from 10.2 to 25.5 % in all samples (depending on the hydrolysis time). Debranched starch samples with a maximum RS yield of 25.5 % were obtained after a debranching time of 24 h. This study showed that starch from the selected ZP maize genotype is suitable for pullulanase treatment and RS preparation but that additional studies with a greater number of different treatments (incubation time/temperature) are necessary to manipulate and promote crystallization and enhance RS formation.
Starch properties and their industrial applications: A review
淀粉特性与工业应用研究进展

包劲松,徐律平,包志毅,傅俊杰
浙江大学学报(农业与生命科学版) , 2002,
Abstract: In this review, the authors introduce the starch properties and summarize the present status of research on starch properties from different botanical sources. The applications of starch and modified starch and the future direction of the research on raw and modified starch are addressed.
SIMULTANEOUS PRETREATMENT OF LIGNOCELLULOSE AND HYDROLYSIS OF STARCH IN MIXTURES TO SUGARS
Hamzeh Hoseinpour,Keikhosro Karimi,Hamid Zilouei,Mohammad J. Taherzadeh
BioResources , 2010,
Abstract: Mixtures of starch and lignocelluloses are available in many industrial, agricultural, and municipal wastes and residuals. In this work, dilute sulfuric acid was used for simultaneous pretreatment of lignocellulose and hydrolysis of starch, to obtain a maximum amount of fermentable sugar after enzymatic hydrolysis with cellulase and β-glucosidase. The acid treatment was carried out at 70-150°C with 0-1% (v/v) acid concentration and 5-15% (w/v) solids concentration for 0-40 minutes. Under the optimum conditions, obtained at 130°C, 1% acid, and 7.5% solids loading for 30 min, the starch was almost completely converted to glucose. However, the acid treatment was not successful for efficient hydrolysis of pure cellulose. A mixture of pine softwood and potato as representatives of lignocellulosic and starch components, respectively, were treated at the optimum conditions for acid hydrolysis of starch. The dilute-acid treatment resulted in 1.2, 60.5, and 23.6% hydrolysis of glucan, xylan, and mannan of pine wood and 67% of potato starch to fermentable sugars. After the acid treatment, the solid residue of the mixture was subjected to enzymatic hydrolysis. The enzymatic hydrolysis under the optimum conditions resulted in conversion of 76% of the glucan in the treated softwood. Therefore, using acid treatment of the mixture is a promising process for pretreatment of wood in addition to the hydrolysis of starch.
PRODUKSI SIKLODEKSTRIN DARI PATI GARUT MENGGUNAKAN BERBAGAI KOMBINASI ENZIM [Production of Cyclodextrin from Arrowroot Starch by Using the Combination Enzymes]  [cached]
Erliza Noor*,Liesbetini Hartoto
Jurnal Teknologi dan Industri Pangan , 2011,
Abstract: The research was aimed to produce cyclodextrin from arrowroot starch using a combination of starch hydrolysis enzymes (α-amylase, α-amylase, pullulanase and glucoamylase). Cyclization process to form cyclodextrin was obtained using Cyclodextrin-glycosyl-transferase (CGTase). α-amylase showed better performance and faster to hydrolyze arrowroot starch as compared to that of β-amylase. Glucoamylase also was gave better result than pullulanase to break the α-1,6-glycosidic chain. Combination of α-amylase and glucoamylase were more efficient for hydrolysis process and cyclodextrin production.
Physicochemical and Functional Characterization of Mucuna pruries Depigmented Starch for Potential Industrial Applications  [PDF]
Maira Rubi Segura-Campos, Sonia Marina López-Sánchez, Arturo Castellanos-Ruelas, David Betancur-Ancona, Luis Chel-Guerrero
International Journal of Organic Chemistry (IJOC) , 2015, DOI: 10.4236/ijoc.2015.51001
Abstract: Starch is a very important biopolymer in the food industry. The velvet bean (M. pruriens) is an excellent potential starch source containing approximately 520 g starch per kg. The objective of this study was to evaluate the physicochemical and functional properties of velvet bean depigmented starch. The starch granules appear oval and spherical shaped. The colour registered L*, a*, b* values of 44.9, 0.324 and 0.341 respectively. The chemical composition registered values of moisture, ash, fat, protein, fibre and NFE of 110.5, 5.8, 5.7, 0.0, 34 and 954.5 g/kg respectively, as well as amylose levels of 215.3 g/kg. Gelatinization onset (To), peak (Tp) and final (Tf) temperatures were of 74.23°C, 80.57°C and 86.39°C. The solubility (3.1% - 16.2%), swelling power (SP) (2.86% - 16.17%) and water absorption capacity (WAC) (2.67 - 15.95 g water/g starch) were directly correlated to temperature (60°C - 90°C). The enthalpy values (4.10 - 13.47 j/g) were directly correlated to the time (1 - 21 days). The retrogradation increased as time increased. The viscosity of M. pruriens depigmented starch decreased slightly during the heating stages and then increased during cooling and the refrigeration and freezing stability registered syneresis ranges from 17.65 to 23.18 mL/50mL and from 16.4 to 22.6 mL/50mL respectively, indicating that the depigmented starch was unstable in heating-cooling processes.
Application of Statistical Method for Screening of Factors Influencing the Production of #-cyclodextrin from Sago Starch Using Combination of Pullulanase and CGTase Enzymes
Suzana Muhamad Taib,Wan Zawiah Wan Zin,Marina Zahari Rosli Md.Illias,Osman Hassan
Journal of Food Technology , 2013,
Abstract: The production of $-cyclodextrin ($-CD) from sago starch was carried out using combination of debranching enzyme and Cyclodextrin Glucanotransferase (CGTase). The contribution of each reaction parameters was evaluated and optimised using statistical modelling. A Fractional Factorial Design (FFD) was employed to screen the effect of substrate concentration, CGTase concentration, pullulanase concentration, pH and temperature for CGTase reaction on the production of $-cyclodextrin ($-CD) using combination of pullulanase and CGTase. The result of first-order factorial design showed that pH and pullulanase had significant positive effect (p<0.05) to the reaction. Substrate concentration, CGTase concentration and temperature exhibited insignificant effect in this reaction. In addition, interaction between CGTase and pH, substrate and pH, pullulanase and temperature, CGTase and temperature, substrate and CGTase gave significant effects (p<0.05) to the $-CD production.
EFFECT OF MEDIA COMPOSITION AND CULTURE CONDITION ON PULLULANASE ENZYME FROM EXTREMOPHILE BACTERIAL SPECIES  [cached]
Ramdas Malakar,Mahaveer Yadav,S. N. Malviya,Archna Tiwari
International Journal of Biomedical and Advance Research , 2012, DOI: 10.7439/ijbar.v3i9.584
Abstract: Pullulanase is of great significant due to its wide area of potential application. Pullulanase is widely used in industries in the saccharification of starch. It converts starch into glucose and maltose which are used in the production of glucose syrup more efficiently. In this present study the enzyme was isolated by the extremophilic strains of Streptococci isolated from extreme conditions. Pullulanase enzyme 98 KDa was successfully produced and purified by the isolated strains. In this process to study enzyme activity the enzyme substrate reaction was performed based on various parameters temperature 50 °C, time 72 hours, and pH 6. As it is clearly stated after the detail analysis of all parameters that the pullulanase isolated in the present study is very stable and active at different parameters. The Pullulanase activity of Gel filtration column chromatography of precipitated protein by ammonium sulphate gave rise 130.66 IU pullulanase activity which was greater than the 0-30% (98.0 IU) and 70-90% (130.66 IU). And thus it can be used in various industrial processes where normal mesophilic enzymes cannot be applied.
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