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Mitotic index in down syndrome - Is it an indicator of immunological status  [cached]
Ranganath Priya,Suresh Geetha,Subramaniam Amudha,Rajangam Sayee
Indian Journal of Human Genetics , 2004,
Abstract: Down syndrome (DS) is a condition in which the genes on chromosome 21 occur in three copies. This is supposed to influence the growth in the tissues of the body and this could lead to a decreased mitotic index. In view of this, the present investigation was carried out using peripheral lymphocyte culture to find out whether there is a change in mitotic index in DS patients. Mitotic index in male and female patients was reduced to an average of 3.64 and 3.82 respectively. Thus, the index could be an indicator of the reduced immunological status of the individual with DS.
Co-overexpression of bcl-2 and c-myc in uterine cervix carcinomas and premalignant lesions  [cached]
Z. Protrka,S. Arsenijevic,A. Dimitrijevic,S. Mitrovic
European Journal of Histochemistry , 2011, DOI: 10.4081/ejh.2011.e8
Abstract: To establish the role of co-overexpression of bcl-2 and c-myc protooncogenes in uterine cervix carcinogenesis, we examined 138 tissue samples of low grade cervical squamous intraepithelial lesions (SIL), high grade SIL, portio vaginalis uteri (PVU) carcinoma in situ and PVU carcinoma invasive, stage IA-IIA (study group) and 36 samples without SIL or malignancy (control group). The expression of bcl-2 and c-myc was detected immunohistochemically using a monoclonal antibody. Fisher’s exact test (P<0.05) was used to assess statistical significance. Overexpression of bcl-2 was found to increase in direct relation to the grade of the cervical lesions. High sensitivity was of great diagnostic significance for the detection of these types of changes in the uterine cervix. On the basis of high predictive values it can be said that in patients with bcl-2 overexpression there is a great possibility that they have premalignant or malignant changes in the uterine cervix. Co-overexpression of bcl-2 and c-myc oncogenes was found only in patients with PVU invasive carcinoma (6/26-23.0%). Statistically significant difference was not found in the frequency of co-overexpression in patients with PVU invasive carcinoma in relation to the control group (Fisher’s test; P=0.064). The method's sensitivity of determining these oncogenes with the aim of detecting PVU invasive carcinoma was 23%, while specificity was 72.2%. On the basis of high predictive values (100%), speaking in statistical terms, it can be concluded that all patients with co-overexpression of bcl-2 and c-myc oncogenes will have PVU invasive carcinoma. We confirmed in our research that co-overexpression of bcl-2 and c-myc oncogenes was increased only in PVU invasive carcinoma. However, a more extensive series of samples and additional tests are required to establish the prognostic significance of bcl-2 and c-myc co-overexpression in cervical carcinogenesis.
Detection of HSV-2 antigen in carcinoma cervix and premalignant conditions by immuno-cytochemistry.  [cached]
Pandit A,Khilnani P,Powar H,Bhave G
Journal of Postgraduate Medicine , 1990,
Abstract: One hundred and twelve cases of cervical pathology (58 paraffin sections and 54 cervical smears) were assessed by PAP technique and 30 cases by indirect immunofluorescent technique. Forty-two normal cervical smears from the age matched controls were stained by indirect immuno-fluorescent technique. HSV-2 antigen was detected by PAP method in 86 out of 112 cases (78.57%) i.e. 50/55 squamous cell carcinoma, 13/13 carcinoma in situ, 11/15 severe dysplasia, 3/4 moderate dysplasia and 9/16 mild dysplasia. The amount of antigen was maximum in squamous cell carcinoma and decreased in carcinoma in situ, severe, moderate and mild dysplasia in descending order. Three cases of adenocarcinoma cervix were negative. Only one case out of 42 controls was positive. HSV-2 antigen was detected by an indirect IF technique in 8/9 squamous cell carcinoma, 2/3 carcinoma in situ, 3/7 dysplasia, O/1 adenocarcinoma and 4/10 inflammatory cases. The above findings support the association between HSV-2 and squamous cell carcinoma cervix, as well as carcinoma in situ which is statistically significant.
Mitotic index and chromosomal analyses for hydroxyapatite implantation in rabbits
T.P. Kannan,B.B. Quah,Azlina Ahmad,A.R. Samsudin
Archives of Orofacial Sciences , 2006,
Abstract: Dentistry has searched for an ideal material to place in osseous defects for many years. Endogenous bone replacement has been the golden standard but involves additional surgery and may be available in limited quantities. Also, the exogenous bone replacement poses a risk of viral or bacterial transmission and the human body may even reject them. Therefore, before new biomaterials are approved for medical use, mutagenesis systems to exclude cytotoxic, mutagenic or carcinogenic properties are applied worldwide. The present preliminary study was carried out in five male New Zealand white rabbits (Oryctolagus cuniculus). Porous form of synthetic hydroxyapatite granules (500 mg), manufactured by School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, Penang, was implanted in the femur of the rabbits. Blood samples were collected prior to implantation and one week after implantation. The blood was cultured in vitro and the cell division was arrested at metaphase using colcemid. This was followed by the hypotonic treatment and fixation. Then, the chromosomes were prepared and stained for analysis. The modal chromosome number of rabbit (Oryctolagus cuniculus) was found to be 2n=44. The mean mitotic index values prior to and after implantation were 3.30 ± 0.66 and 3.24 ± 0.27 per cent respectively. No gross chromosome aberrations, both numerical and structural were noticed either prior to or after implantation of the biomaterial. These findings indicate that the test substance, synthetic hydroxyapatite granules does not produce gross chromosome aberrations under the present test conditions in rabbits.
Effects of caffeine on mitotic index in Drosophila prosaltans (Diptera)
Itoyama, Mary Massumi;Bicudo, Hermione Elly Melara de Campos;Cordeiro, José Ant?nio;
Brazilian Journal of Genetics , 1997, DOI: 10.1590/S0100-84551997000400016
Abstract: the effect of two concentrations of caffeine (1500 mg/ml and 2500 mg/ml) on mitotic indices of drosophila prosaltans was analyzed in larval brain cells. although the differences detected between treated and control cells were not significant, the percentages obtained suggest a possible effect of caffeine in slowing the process of cell division
Effects of caffeine on mitotic index in Drosophila prosaltans (Diptera)  [cached]
Itoyama Mary Massumi,Bicudo Hermione Elly Melara de Campos,Cordeiro José Ant?nio
Brazilian Journal of Genetics , 1997,
Abstract: The effect of two concentrations of caffeine (1500 mg/ml and 2500 mg/ml) on mitotic indices of Drosophila prosaltans was analyzed in larval brain cells. Although the differences detected between treated and control cells were not significant, the percentages obtained suggest a possible effect of caffeine in slowing the process of cell division
Inactivation of SLIT2-ROBO1/2 Pathway in Premalignant Lesions of Uterine Cervix: Clinical and Prognostic Significances  [PDF]
Sraboni Mitra, Dipanjana Mazumder-Indra, Ranajit K. Mondal, Partha S. Basu, Anup Roy, Susanta Roychoudhury, Chinmay K. Panda
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0038342
Abstract: The SLIT2-ROBO1/2 pathways control diverse biological processes, including growth regulation. To understand the role of SLIT2 and ROBO1/2 in cervical carcinogenesis, firstly their RNA expression profiles were screened in 21 primary uterine cervical carcinoma (CACX) samples and two CACX cell lines. Highly reduced expressions of these genes were evident. Concomitant alterations [deletion/methylation] of the genes were then analyzed in 23 cervical intraepithelial neoplasia (CIN) and 110 CACX samples. In CIN, SLIT2 was deleted in 22% samples compared to 9% for ROBO1 and none for ROBO2, whereas comparable methylation was observed for both SLIT2 (30%) and ROBO1 (22%) followed by ROBO2 (9%). In CACX, alteration of the genes were in the following order: Deletion: ROBO1 (48%) > SLIT2 (35%) > ROBO2 (33%), Methylation: SLIT2 (34%) > ROBO1 (29%) > ROBO2 (26%). Overall alterations of SLIT2 and/or ROBO1 (44%) and SLIT2 and/or ROBO2 (39%) were high in CIN followed by significant increase in stage I/II tumors, suggesting deregulation of these interactions in premalignant lesions and early invasive tumors. Immunohistochemical analysis of SLIT2 and ROBO1/2 in CACX also showed reduced expression concordant with molecular alterations. Alteration of all these genes predicted poor patient outcome. Multiparous (≥5) women with altered SLIT2 and ROBO1 along with advanced tumor stage (III/IV) and early sexual debut (<19 years) had worst prognosis. Our data suggests the importance of abrogation of SLIT2-ROBO1 and SLIT2-ROBO2 interactions in the initiation and progression of CACX and also for early diagnosis and prognosis of the disease.
The Effect of Bleomycin to the Mitotic Index who were Exposed to Radiation Chronically  [PDF]
Hilmi ?si,Diclehan ?ktüren Oral,Ay?egül Bengisu Türky?lmaz,Mahmut Balkan
Dicle Medical Journal , 2004,
Abstract: Beginning with the therapeutic dose and increasing three differentdoses and three different periods of time Bleomycin (Bleomycin 0.3 μg/ml, 3μg/ml and 30 μg/ml, 6, 24, 48 hours) were added to blood which was beingcultured on 5 samples who were exposed to radiation chronically.The mitotic index in the lymphocyte culture was determined by means ofcounting 1000 cells from preparations belong to control and experimentgroups.The mitotic index was showed difference value to the differencebleomycin dose and the between difference samples. As the mitotic indexvalue (Per 1000 cell) 10.86 for the control group, this value fall to 8.73 at0.3 μg/ml, 5.0 at 3 μg/ml and 3.93 at 30 μg/ml. There were no significantdifferences between control group and 0.3 μg/ml, 3 μg/ml and 30 μg/ml(P>0.05). An important fall was seen between control group and 3 μg/mland 30 μg/ml, 0.3 μg/ml group and 3 μg/ml, 30 μg/ml (P<0.01).
Based on ECM Modelling for Daily Turnover and Close Index of Chinese Stock Markets
Xiaohua Hu,Min Yu
International Journal of Economics and Finance , 2012, DOI: 10.5539/ijef.v4n11p205
Abstract: By making use of test for stationary, Granger, co-integration, we study the daily turnover and daily close index of Chinese stock markets from 1991 to 2011. We strive to find how Shanghai and Shenzhen stock markets interact each other, there really exist a long-run equilibrium equation among the daily close index,daily turnover of Shanghai (Shenzhen) market and daily close index of Shenzhen (Shanghai) market, to establish the two-order bivariate error correction model(ECM)for two Chinese stock markets respectively. We also further analyze the act of the fluctuation of daily close index of the two markets in short-term.
Mitotic index studies on edible cocoyams (Xanthosoma and Colocasia spp.)
AM Ekanem, JO Osuji
African Journal of Biotechnology , 2006,
Abstract: Mitotic index studies were carried out on three cultivars of Xanthosoma and four cultivars of Colocasia. Young healthy roots (about 15 mm) were collected at 2 hourly intervals from 6:00 am to 8:00 pm. Root tips were fixed in 1:3 ethanol : acetic acid for 24 h and stored in 70% ethanol prior to squashing in FLP orcein. Microscopic counts showed that the dynamics of mitosis varied slightly between the two groups of cultivars. The peak of metaphase remained between 12:00 noon and 2:00 pm for most of cultivars but one (NCY 00Sa), which had its metaphase rising to a peak between 2:00 and 4:00 pm. This suggests that the best time to harvest root samples for optimum metaphase is immediately before 12:00 noon
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