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Research: STUDY THE POSSIBLE HEPATOPROTECTIVE EFFECT OF DIFFERENT DOSES OF Ammi majus SEEDS' EXTRACT AGAINST CCl4 INDUCED LIVER DAMAGE IN RATS
Shihab Hattab Mutlag,Dawser K Ismael,Nada N Al-Shawi*
Pharmacie Globale : International Journal of Comprehensive Pharmacy , 2011,
Abstract: Liver is considered as the major organ responsible for conducting various metabolic processes and according to it’s highly exposed to toxic effect of different xenobiotics predisposing to many types of diseases and disorders. The role of plant with antioxidant activity in the treatment and prevention of chemical-induced liver damage was extensively studies. Ammi majus show antioxidant effect their use in diabetic nephropathy and myocardial injury due to the presence of different active constituent such as quercetine, marmesinin, kempefrol and other compounds that inhibit cytochrome p450 such as xanthotoxin bergapten, imperatorin and isoimpinellin. Accordingly, this study was designed to evaluate the hepatoprotective effect of the aqueous solution of ethanolic extract of the Ammi majus against carbon tetrachloride (CCl4) induced liver damage in rats. Eighty adult rats of both sex divided into four groups allocated as follows: Group I- (negative control), rats received D.W 2ml/kg for 14 days. Group II- rats treated with single oral daily dose Ammi majus seeds extract 16mg/rat/day alone for 14 days. The animals of groups I and II were sacrificed by anesthetic ether on the day 15. Group III- (positive control) rats received single oral daily dose of 2ml/kg/day D.W. for 14 days and at the day 15, the animals received single oral dose of CCl4, the animals were sacrificed by anesthetic ether 24 hr after CCl4 administration. Groups IV (A, B, C, D and E) received either (1mg or 2mg or 4mg or 8mg or 16mg/rat/day), respectively for 14 days of Ammi majus ethanolic extract then at the day 15 they received single dose of CCl4 then sacrificed after 24 hours after CCl4 administration. Rats' livers were obtained for preparation of tissue homogenate to evaluate MDA & GSH in the hepatic homogenate as indicator of lipid peroxidation. Blood samples were collected by intra-cardiac puncture, and utilized for evaluating serum enzymes activities manifested by aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in addition for assessing total serum Bilirubin (TSB). Analysis of data revealed significant amelioration of oxidative stress in rats pre-treated with different doses of Ammi majus extract (4mg,8mg and 16mg/rat/day for 14days) compared to group III of animals intoxicated by CCl4 as evidenced by lowering MDA contents and elevation of GSH levels in liver tissue homogenate but the levels still significantly different compared to controls. Elevation of serum activities of ALT, AST and ALP, in addition to TSB levels as a results of t
Extraskeletal Chondrosarcoma of Labium Majus
Arshad S. Khan,Girish D. Bakhshi,Aftab Shaikh,Ashraf A. Khan,Adil A. Khan,Arun Chitale
Case Reports in Pathology , 2011, DOI: 10.1155/2011/429562
Abstract: Extraskeletal myxoid chondrosarcoma (ESMC) is a rare tumor seen more often in men. It is seen to arise from soft tissue of lower extremity or buttocks. We report a case of soft tissue swelling of left labium majus in a 66-year-old female. Patient underwent wide excision with uneventful postoperative course. Histopathology of specimen confirmed it to be ESMC. Patient refused adjuvant therapy. Followup of 1 year has shown her to be disease- and symptom- free. Only two cases arising from vulva have been reported in literature . This is the third case and first from Indian subcontinent. A brief review of clinical features, diagnosis, treatment, and outcome of patients with extraskeletal chondrosarcoma is presented.
AMMI Stability of Some of Internationally Derived Durum Wheat Varieties in the Southeastern of Anatolia  [PDF]
Irfan Ozberk,Fethiye Ozberk,Hans J. Braun
Pakistan Journal of Biological Sciences , 2005,
Abstract: This study aimed to investigate the stability of some of internationally derived durum wheat varieties in the Southeastern Anatolia. The experimental data, collected from both rain fed and supplementary irrigated conditions in different locations of Southeastern of Anatolia in 1995/96,1996/97 and 1997/98 cropping seasons, were subjected to Additive Main Effects and Multiplicative Interactions (AMMI) stability assessment method. The results, obtained from AMMI analysis indicated that Aydin-93 and Ceylan-95 were the most responsive varieties. Harran-95 and Diyarbakir-81 both seemed to be stabile yielding varieties under both rain fed and supplementary irrigated conditions, while, Firat-93 was the least responsive variety, adapted to poor environmental conditions. It was concluded that several responsive and stabile yielding varieties have been developed adapting to a wide range of environments in the Southeastern of Anatolia.
KARYOTYPE AND IDIOGRAM IN CHELIDONIUM MAJUS L  [cached]
Angela Pavel,Maria Butnaru
Analele ?tiin?ifice Ale Universit??ii Alexandru Ioan Cuza din Ia?i,Sectiunea II A : Genetica si Biologie Moleculara , 2003,
Abstract: Cy togenetical studies have been concentrated on the kary oty pe and idiogram making up. All analy sed metaphasis cells have revealed 2n=12 chrom osom es, being according to the literature data. Chelidonium majus kary oty pe is m aked up of five m edian chromosome pairs and a sub edian chrom osom e m pair, being sim metric and prim itive. The first chrom osome pair is considereda identify marker of this species. The C.majus idiogram has included two chrom osome clusters which depend of centrom ere placing and can be synthetized as following: two m edian chrom osom e pairs and four chrom osome pairs belong to submedian type (one pair of them contains secondary constrictions with satellites).
Primary extraskeletal osteosarcoma of omentum majus
Shui-Xiang Tao, Guo-Qin Tian, Meng-Hua Ge, Cheng-Long Fan
World Journal of Surgical Oncology , 2011, DOI: 10.1186/1477-7819-9-25
Abstract: Extraskeletal osteosarcoma (ESOS) is a rare malignant mesenchymal neoplasm in soft tissues but not directly attached to the skeletal system. Fewer than 300 cases have been reported to date. Here, we describe a case of a primary ESOS arising from omentum majus. To the best of our knowledge, this is the second reported case in the English literature.A 40-year-old woman was admitted to our hospital complaining of lower abdominal pain with nausea and vomiting for 4 days. She denied any history of other previous abdominal injuries or pain and had no other pertinent past medical history. The patient's general condition was good and no weight loss was observed. Ultrasonography of the pelvic cavity showed a soft tissue mass with marked calcification, measuring 6.9 × 4.6 cm. Laboratory tests revealed an increase in the alkaline phosphatase (213 u/L) and carbohydrate antigen 125 (102 u/moL). For resection of the tumor, en bloc mass excision with segmental omentum majus resection was performed. During the operation, the mass was located in the lower margin of greater omentum, and tumor invasion into surrounding organs was not observed. There was no lymph node swelling or peritoneal dissemination. About 500 ml of bloody effusion was present in the peritoneal cavity. Frozen section showed a malignant mesenchymal tumor of omentum majus.The resected tumor was 6.0 × 4.0 × 3.0 cm in size. The cut surface consisted of a gray-white to tan-yellow solid area with a gritty sensation. Microscopically, there was calcificied neoplastic osteoid among the tumor cells and osteoclastic giant cells that showed intensive positive reaction with Vimentin and CD99 (histiocytic marker) (Figure 1), but negative for CK, CR, EMA, CD117, CD34, CD68, SMA and Desmin. The final pathological diagnosis was extraskeletal osteosarcoma of omentum majus.The postoperative course was uneventful. We recommended adjuvant radiotherapy and chemotherapy, but the patient refused. Five months after the operation, she was
Deregressed EBV as the response variable yield more reliable genomic predictions than traditional EBV in pure-bred pigs
Tage Ostersen, Ole F Christensen, Mark Henryon, Bjarne Nielsen, Guosheng Su, Per Madsen
Genetics Selection Evolution , 2011, DOI: 10.1186/1297-9686-43-38
Abstract: Reliabilities of genomic breeding values were estimated with EBV and deregressed EBV as response variables and under the three statistical methods, genomic BLUP, Bayesian Lasso and MIXTURE. The methods were examined by splitting data into a reference data set of 1375 genotyped animals that were performance tested before October 2008, and 536 genotyped validation animals that were performance tested after October 2008. The traits examined were daily gain and feed conversion ratio.Using deregressed EBV as the response variable yielded 18 to 39% higher reliabilities of the genomic breeding values than using EBV as the response variable. For daily gain, the increase in reliability due to deregression was significant and approximately 35%, whereas for feed conversion ratio it ranged between 18 and 39% and was significant only when MIXTURE was used. Genomic BLUP, Bayesian Lasso and MIXTURE had similar reliabilities.Deregressed EBV is the preferred response variable, whereas the choice of statistical method is less critical for pure-bred pigs. The increase of 18 to 39% in reliability is worthwhile, since the reliabilities of the genomic breeding values directly affect the returns from genomic selection.Genomic selection in pure-bred pigs can be implemented using a multi-step procedure. Effects of dense genetic markers are estimated using a reference population and these effects are used to predict genomic breeding values (GBV) of selection candidates [1]. Implementing a multi-step procedure relies on two prerequisites: 1) a response variable that summarises the genetic information for reference animals, and 2) a statistical method that associates the response variable to the marker information. The choice of response variable and statistical method may well depend on the data structure. Therefore, the challenge is to find a suitable response variable and statistical method that can handle pure-bred pig data.Pure-bred pig data often have low and varying reliabilities of est
A molecular recombination map of Antirrhinum majus
Zsuzsanna Schwarz-Sommer, Thomas Gübitz, Julia Weiss, Perla Gómez-di-Marco, Luciana Delgado-Benarroch, Andrew Hudson, Marcos Egea-Cortines
BMC Plant Biology , 2010, DOI: 10.1186/1471-2229-10-275
Abstract: We created a molecular linkage map of A. majus based on segregation of markers in the F2 population of two inbred lab strains of A. majus. The resulting map consisted of over 300 markers in eight linkage groups, which could be aligned with a classical recombination map and the A. majus karyotype. The distribution of recombination frequencies and distorted transmission of parental alleles differed from those of a previous inter-species hybrid. The differences varied in magnitude and direction between chromosomes, suggesting that they had multiple causes. The map, which covered an estimated of 95% of the genome with an average interval of 2 cM, was used to analyze the distribution of a newly discovered family of MITE transposons and tested for its utility in positioning seven mutations that affect aspects of plant size.The current map has an estimated interval of 1.28 Mb between markers. It shows a lower level of transmission ratio distortion and a longer length than the previous inter-species map, making it potentially more useful. The molecular recombination map further indicates that the IDLE MITE transposons are distributed throughout the genome and are relatively stable. The map proved effective in mapping classical morphological mutations of A. majus.Antirrhinum majus, the garden snapdragon, has been used as a model system for genetics since the early 20th Century [1]. It is a member of a monophyletic group of about twenty five species that are native to the Mediterranean region share the same chromosome number (2n = 16) and are able to form fertile hybrids with each other [2]. The majority of species are allogamous, though cultivated A. majus lines and a few other wild species can self-fertilize.A collection of A. majus mutants has been produced from some laboratory lines of A. majus selected for high transposon activity [3]. In several cases, these have been used to clone the corresponding genes by transposon tagging (e.g. [4-10]). In addition there is a colle
Phytosteroids from tissue culture of Allium cepa L. and Trachyspermum ammi S prague.  [PDF]
Pratibha Chaturvedi,Pushpa Khanna
Journal of Pharmacognosy and Phytochemistry , 2013,
Abstract: Production of secondary metabolites by cultured cells provides a particularly important benefit to manipulate and improve the production of desired compounds; thus biotechnological approaches to increase the concentrations of the metabolites are discussed. Present study deals with the production, isolation and identification of phytosterols from tissue culture of Allium cepa and from plant parts and tissue culture of Trachyspermum ammi. Steroidal analysis of plant parts showed the maximum amount of stigmasterol (0.240 mg/gdw) which was comparatively little less than that of the amount of β- sitosterol (0.295 mg/gdw) in the seeds of T. ammi . The maximum amount of stigmasterol was present in four weeks old tissue of T. ammi (0.249 mg/gdw) whereas the highest content of β- sitosterol was observed in six weeks old tissue of A. cepa (0.315 mg/gdw) However, lanosterol, was present only in the tissue of A. cepa which was maximum in six weeks old tissue (0.039 mg/gdw).
Chromoplast biogenesis in Chelidonium majus petals
Nikola Ljube?i?,Mercedes Wrischer
Acta Societatis Botanicorum Poloniae , 2006, DOI: 10.5586/asbp.2006.013
Abstract: The differentiation of chromoplasts, with special emphasis on the formation and the organisation of chromoplast fibrils, was followed in the petals of the greater celandine, Chelidonium majus L. Electron microscopic observations showed that, in the epidermis, differentiation of chromoplasts started from leucoplasts, while mesophyll chromoplasts originated from chloroplasts. During petal maturation, fibrils accumulated in the plastids, often arranging in a parallel fashion to form compact birefringent bundles. Immediately before flower opening, these fibrillar bundles started to disorganise, and, at anthesis, most chromoplasts contained widely spaced fibrils which were irregularly dispersed through the plastid interior. During chromoplast differentiation, fibrils were commonly observed to protrude from plastoglobules, suggesting the possible site of their formation. Western analysis indicated that a protein antigenically related to fibrillin from pepper chromoplasts participates in the constitution of fibrils in Chelidonium petals.
Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants
Tae-Jin Kang, Moon-Sik Yang
BMC Biotechnology , 2004, DOI: 10.1186/1472-6750-4-20
Abstract: We developed new rapid and reliable genomic DNA extraction method. With our developed method, plant genomic DNA extraction could be performed within 30 min. The method was as follows. Plant tissue was homogenized with salt DNA extraction buffer using hand-operated homogenizer and extracted by phenol:chloroform:isoamyl alcohol (25:24:1). After centrifugation, the supernatant was directly used for DNA template for PCR, resulting in successful amplification for RAPD from various sources of plants and specific foreign genes from transgenic plants. After precipitating the supernatant, the DNA was completely digested by restriction enzymes.This DNA extraction procedure promises simplicity, speed, and efficiency, both in terms of time and the amount of plant sample required. In addition, this method does not require expensive facilities for plant genomic DNA extraction.Molecular biological studies of plants require high-quality DNA. Several DNA extraction procedures for isolating genomic DNA from various plant sources have been described, including the salt extraction method and the cetyltrimethyl ammonium bromide (CTAB) method [1] and its modifications [2,3]. The need for a rapid and simple procedure is urgent, especially when hundreds of samples need to be analyzed.Most methods require the use of liquid nitrogen [4] or freeze-drying (lyophilization) [5,6] of tissue for the initial grinding, and these processes are unavailable in many regions of the world. After grinding the tissues in various extraction buffers, DNA is extracted with phenol-chloroform, or the extract is dialyzed against EDTA and a buffered Tris-HCl solution [7]. After extraction, the aqueous phase is concentrated, either by ethanol or isopropanol precipitation [8,9], or with microconcentrators (e.g., the Wizard genomic DNA purification system; Promega, USA). However, these methods are not time efficient for consistently obtaining PCR-quality DNA from calluses and plants, since they require that the tissu
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