The Maki-e technique with eggshell powder is one of techniques of Japanese traditional Urushi (Japanese lacquer) crafts. However, this technique is relatively new in the history of Maki-e, and there are no prior researches in terms of materials, structure, and properties. In this research, therefore we have aimed to evaluate the relationship between the eggshell powder sizes, dispersion, and color shade in Maki-e with eggshell powder. The difference between hen’s and quail’s eggshell characteristics and the effect of their powder particle size on appearance of eggshell Maki-e were discussed on the basis of the results of the particle size, circularity, particle number, and RGB value. As a result, it was found that the occupancy of the eggshell powder on the surface depends on not particle number but the particle size, whereas the whiteness of both eggshell powders depends on the particle size.