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In Vitro Fertilization Outcomes Following Assisted Hatching of Embryos with Thick Zona Pellucida—A Prospective Randomized Study  [PDF]
Mara Koifman, Shirly Lahav-Baratz, Libby Shopen, Blais Idit, David Ishai, Zofnat Wiener-Megnazi, Ron Auslender, Martha Dirnfeld
Advances in Reproductive Sciences (ARSci) , 2014, DOI: 10.4236/arsci.2014.24009
Abstract: Purpose: Impaired hatching is associated with implantation failure following in vitro fertilization (IVF). Thickening or hardening of the zona pellucida (ZP) has been proposed as a factor in this impairment. We examined whether selective assisted hatching (AH) is beneficial with embryos having a thick ZP. Methods: This prospective, randomized controlled study was performed in the IVF unit of an obstetrics and gynecology department in a university-affiliated hospital. Only patients undergoing IVF and having a ZP thickness of ≥17 μm measured in all their embryos were included. In the intervention group, AH was applied to all embryos, before their transfer. In the control group, embryos were transferred without AH. Implantation, clinical pregnancy and live birth rates were the study endpoints. Results: Both study arms were comparable in most baseline parameters. The two groups did not differ in implantation rates (14.1% control vs. 8.92% intervention, odds ratio (OR) = 0.5974, 95% confidence interval (CI) 0.325 - 1.1), clinical pregnancy rates (36.7% vs. 25.8%, OR = 0.6025, 95% CI 0.274 - 1.325), or live birth rates (25% vs. 18.9%, OR = 0.7021, 95% CI 0.291 - 1.691). Conclusions: Selecting embryos for AH by their ZP thickness as a sole parameter was not found to be beneficial and to improve IVF outcome.
The effect of different cold period during maternal incubation on incubation efficiency and hatching term in Austropotamobius pallipes  [PDF]
T. Policar,J. Smyth,M. Flanigan,P. Kozak
Knowledge and Management of Aquatic Ecosystems , 2009,
Abstract: This study tested the effect on the incubation efficiency (E in %) and hatching term during maternal incubation of Austropotamobius pallipes of five different cold periods (duration: 45, 60, 75, 90 and 105 days) under controlled conditions and one group maintained under ambient Irish water temperatures. The six different durations of cold period, used in this study, caused six different terms of hatching from 16 March to 29 June. When compared to the group held under ambient Irish conditions with fluctuating water temperatures during the incubation period (E = 29.9 ± 4.5%), higher incubation efficiency was found in all groups under the controlled conditions (E = 73.1 ± 4.7% 41.3 ± 2.7%). In groups under controlled conditions, a positive effect of shortened cold period on incubation efficiency was found, with the highest efficiency (E = 73.1 ± 4.7% 68.8 ± 5.2%) found after the shortest cold period, while the longest cold period led to the lowest efficiency (E = 41.3 ± 2.7%).

Zhou Zhengjin Pu Qun Lai Peihua Bai Xiuqing,

力学学报 , 1991,
Abstract: The influence of temperature on cross-hatching on cone models made of wax and camphor is studied. Ablating experiments are carried out in heated supersonic tunnels. The relation between temperature and the wavelength of hatching is obtained. The relation between pressure and the wavelength of hatching is verified. The influence of temperature and pressure on the initial position of the appearance of hatching and on the time required for both the appearance of hatching and the formation of stable hatching analyzed.
Long-term memory was impaired in one-trial passive avoidance task of day-old chicks hatching from hypomagnetic field space
Xuebin Wang,Muling Xu,Bing Li,Dongfeng Li,Jinchang Jiang
Chinese Science Bulletin , 2003, DOI: 10.1360/03wc0231
Abstract: The retained curve in one-trial passive avoidance task (OTPAT) of day-old chicks hatching from natural geomagnetic field (control groups) is consistent with the acknowledged three-phase model. The two dips are at the 20th min and the 60 min, and the avoidance rates (AR) to the red bead in short-and intermediate-term memory are 68.4% on average, while that in the long-term momory was 74.8%. The OTPAT retained curve of day-old chicks hatching from hypomagnetic field space (experimental groups) presents the marked timing effect. The two dips were at the 25th min and the 50th min, the avoidance rates to the red bead (AR R ) in the short-and intermediate-term memory was 74.1% on an average, which was resembled to that in control group; however, the long-term memory appeared an obvious fluctuation. Compared with that in control groups, AR R and the deviation coefficient in experimental groups were decreased by 25.3% and increased by 1.3 times, respectively. That is to say, both the memory ability and stability in the experimental chicks declined. The result shows that the hypomagnetic field space has a negative effect on the development of chick brain function, and that cannot be negligible.
Tympanometry, a Prognostic Indicator of Myringoplasty with Assessment of Eustachian Tube Function  [PDF]
Shreyas S. Joshi, Mohan Jagade, Saurabh Agarwal, Dnyaneshwar Ahire
International Journal of Otolaryngology and Head & Neck Surgery (IJOHNS) , 2012, DOI: 10.4236/ijohns.2012.13021
Abstract: Objective: To determine Eustachian tube function in tubotympanic type of chronic ear disease and to correlate Eustachian tube function with pathological change in middle ear mucosa. Materials and Methods: A prospective study of the cases of tubotympanic type of chronic suppurative otitis media was undertaken. Patients were subjected to clinical examination followed by audiometry tests and impedence audiometry to determine eustachian tube functions. Patients were then subjected to myringoplasty and eustachian tube function were then reassessed post operatively. Results: In our study, out of 30 cases who underwent myringoplasty, 21 cases had successful surgical outcome and 9 cases were failed. Out of 24 cases of good Eustachian tube function, 21 cases had successful surgical outcome intends of taking up graft, absence of otorrhea and improvement in hearing. In remaining 3 cases, there was recurrence of otorrhea with central perforation. All the 6 cases with poor Eustachian tube function had surgical failure. Conclusion: The prognosis of the middle ear reconstructive surgery has direct correlation with Eustachian tube functions and Eustachian tube function tests should be a routine investigation in all the cases planned for surgical reconstruction of the middle ear.
The presence of a lipoma in the Eustachian tube: a case report
Zhuofu Liu, Dehui Wang, Quan Liu
Journal of Medical Case Reports , 2011, DOI: 10.1186/1752-1947-5-436
Abstract: We report an incidentally-detected lipoma of the Eustachian tube in a 34-year-old Asian woman with a six-year history of persistent otitis media in her right ear. Our patient underwent surgery five years ago for the possibility of a choanal polyp, but her ear symptoms continued to be problematic following the surgery. Our patient was examined at our hospital, and computed tomography revealed a well-defined, hypodense, non-enhancing lesion involving the right Eustachian tube, measuring 1.6 × 2.4 cm. The mass was excised using an endoscopic approach, and was found to originate from the cartilaginous portion of the Eustachian tube. The tumor was sent for histopathologic evaluation. The postoperative course went smoothly, and our patient recovered during follow-up over the next five months.Lipoma of the Eustachian tube is very rare compared with other tumors. Improved radiologic modalities aid the diagnosis of this benign tumor. Endoscopic removal of the tumor is possible and has helped in early recovery.Lipomas are benign tumors and present as painless soft tissue masses commonly seen in adults [1]. They can arise from many parts of the body, but are most commonly found in the subcutaneous tissue of the neck and trunk. According to their location, they are also categorized as subcutaneous, submucous or intramuscular lipomas. Apart from fat cells, lipomas may also contain other tissue components, such as fibrous, nervous or vascular tissue [2]. Lipomas originating from the Eustachian tube are extremely rare. Herein we report a case of a lipoma arising from the Eustachian tube.A 34-year-old Asian woman presented at our hospital, with a six-year history of persistent right otitis media with effusion. In addition, on presentation, our patient was suffering from tinnitus and a sensation of 'fullness' in her right ear. Our patient underwent unilateral myringotomy through the insertion of a pressure equalization tube, which was kept in place up until a few months ago because
Morphology and Ciliary Motion of Mucosa in the Eustachian Tube of Neonatal and Adult Gerbils  [PDF]
Yi Li, Huizhan Liu, Jun Li, Qian Zhang, Shusheng Gong, David He
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0099840
Abstract: The Eustachian tube is a small canal that connects the tympanic cavity with the nasal part of the pharynx. The epithelial lining of the Eustachian tube contains a ciliated columnar epithelium at the tympanic cavity and a pseudostratified, ciliated columnar epithelium with goblet cells near the pharynx. The tube serves to equalize air pressure across the eardrum and drains mucus away from the middle ear into the nasopharynx. Blockage of the Eustachian tube is the most common cause of all forms of otitis media, which is common in children. In the present study, we examined the epithelial lining of the Eustachian tube in neonatal and adult gerbils, with a focus on the morphological and functional development of ciliated cells in the mucosa. The length of the tube is ~8.8 mm in adult gerbils. Scanning electron microscopy showed that the mucosal member near the pharyngeal side contains a higher density of ciliated cells and goblet cells than that near the tympanic side. The cilia beat frequency is 11 Hz. During development, the length of the Eustachian tube increased significantly between postnatal day 1 (P1) and P18. Scanning electron microscopy showed that the mucosa contained a high density of ciliated cells with a few goblet cells at P1. The density of ciliated cells decreased while the density of goblet cells increased during development. At P18, the mucosa appeared to be adult-like. Interestingly, the ciliary beat frequency measured from ciliated cells at P1 was not statistically different from that measured from adult animals. Our study suggests that the Eustachian tube undergoes significant anatomical and histological changes between P1 and P18. The tube is morphologically and functionally mature at P18, when the auditory function (sensitivity and frequency selectivity) is mature in this species.
Long-Term Outcomes of Balloon Dilation for Acquired Subglottic Stenosis in Children  [PDF]
Aliye Filiz,Seckin O. Ulualp
Case Reports in Otolaryngology , 2014, DOI: 10.1155/2014/304593
Abstract: Objectives. Balloon dilation laryngoplasty has been suggested as an alternative treatment to open surgical treatment of acquired subglottic stenosis in children. We describe long-term outcomes of balloon dilation for acquired subglottic stenosis in children. Methods. The medical charts of children who had balloon dilation for subglottic stenosis secondary to intubation were reviewed. Data included demographics, relevant history and physical examination, diagnostic workup, and management. Outcomes of balloon dilation were assessed based on improvement in preoperative symptoms, grading of stenosis, complications, and need for additional procedures. Results. Three children (2 male, 1 female, age range: 14 weeks–1 year) underwent balloon dilation for acquired subglottic stenosis. Patients presented with stridor and increased work of breathing. Duration of intubation ranged from 2 days to 3 weeks. Patients became symptomatic 5 days to 6 weeks after extubation. Grade of subglottic stenosis was II in 2 patients and III in one. Subglottic stenosis patients had 2-3 dilations within 2–10 weeks. All patients were asymptomatic during 14–21-month follow-up. Conclusions. Serial balloon dilation was safe and successful method to manage acquired subglottic stenosis in this group of children. No recurrence was noted in a follow-up more than a year after resolution of symptoms. 1. Introduction Acquired subglottic stenosis in children has been treated using a wide variety of approaches such as observation, dilation, laser, single or multistage open surgical reconstructions, and tracheostomy. With the advent of high-pressure, noncompliant airway balloons, balloon laryngoplasty has been increasingly used for treatment of acquired subglottic stenosis in children. Balloon dilation of the airway provides an opportunity to apply radial force to subglottic narrowing and prevents shearing forces created by rigid dilators [1]. Serial balloon dilations may be needed and open procedures may be avoided in some patients. Outcomes of balloon dilation in children have been increasingly investigated. Nevertheless, long-term outcomes of balloon dilation for acquired subglottic stenosis have not been established in children. The aim of the present study is to describe long-term outcomes of balloon dilation for acquired subglottic stenosis in children. 2. Case Reports The medical charts of children who had balloon dilation for subglottic stenosis secondary to intubation were reviewed. Data included demographics, relevant history and physical examination, diagnostic workup, and management.
Transobturator Tape Technique For Stress Urinary Incontinance and Long Term Outcomes  [cached]
Musa Ekici,Hasan Nedim Goksel Goktug,Nevzat Can Sener,Ismail Nalbant
Cukurova Medical Journal , 2013,
Abstract: Purpose: Even though vairous surgical procedures are developed to treatment of stress urinary incontinance, there has not been yet a gold standard treatment. In this study, we aimed to discuss the application of TOT technique, its complications, safety and the six year follow up results. Material And Methods: 30 patients applied to our clinic and diagnosed SUI were performed TOT. Mean patient age was 49,9±9,4 (23-64). Demographic parameters, physical and pelvic examination, q tip test, stress test, cystoscopy, ICIQ-SF score, duration of surgery, intraoperative complications, early and late postoperative complications were recorded. Mean follow up was 72,5±7,1 (44-88) months. Patients were evaluated after 1, 3, 6, 12 and 24 months after surgery. Operations were performed by outside-inside method and by using Safyre(promedon). The outcomes were analysed using Shapiro wilk, paired sample t test, Friedman test and Wilcoxon test. Outcomes: Mean operation time and hospital stay were 21,8(15-35) and 1,3 days(1-2) respectively. Peroperatively, one patient(3,3%) had her vaginal wall pierced by the needle. Bladder, uretral or vascular damage were not seen in any patients. Self catheterization was required for only one (3,3%) patient. At late postoperative period, 1 patient developed de novo urge incontinence (3,3%) and one patient developed (3,3%) left leg pain. 73% of the patients with a median follow up of 72,5±7,1 (44-88) months were dry. Preopearative ICIQ-SF median value of 12.4 (8-20) were decreased to 1.7 (0-6), and similarly, ped use was decreased from 2,9 (1-8) to 0,4 (0-3). Results: TOT is a safe and effective treatment of SUI with its long term outcomes. However, long term outcomes with comparison with other techniques and including higher cohorts is required. [Cukurova Med J 2013; 38(4.000): 574-580]
Amantadine-Induced Patulous Eustachian Tubes in Parkinson’s Disease  [PDF]
J. T. Boyd,D. A. Silverman
Case Reports in Otolaryngology , 2013, DOI: 10.1155/2013/426413
Abstract: Patulous Eustachian tube (PET) is a common condition that produces symptoms of aural fullness and autophony. We describe a Parkinson’s disease (PD) patient that experienced a reversible bilateral patulous (hyperpatent) Eustachian tube syndrome induced by treatment with amantadine hydrochloride. The clinical features, relevant anatomy and physiology, and associated risk factors for PET are reviewed. 1. Introduction Patulous Eustachian tube (PET) is a common condition that presents with the primary complaint of distorted or echoed autophony. Autophony is the amplified auditory perception of self-generated sounds such as respiration, vocalization, and swallowing [1–3]. Additional presenting features can include a feeling of aural fullness, mild vertigo or imbalance, recurrent nasal sniffing to relieve autophony, and positional symptom improvement when supine. Persistent communication of the pharynx and middle ear occurs in the setting of impaired closure of the Eustachian tube at the nasopharyngeal orifice [4, 5]. Previously reported contributing factors include weight loss, caffeine use, pregnancy, and medications including diuretics and nasal decongestants [3–7]. Neurological disorders that have been associated with PET include motor neuron disease, multiple sclerosis, and stroke [3–8]. In healthy cases, the ET closes passively via recoil of the elastin hinge and force generated by the deformation of Ostmann’s fat pad. Opening occurs during yawning, sneezing, and deglutition actively by contraction of the tensor veli palatini and passively by the levator veli palatini. Mucus producing goblet cells line the medial two-thirds of the ET near the pharyngeal orifice [3, 7]. 2. Case Presentation We report the case of a patient who developed PET induced by amantadine hydrochloride which resolved with discontinuation. The patient is a 50-year-old man with a one-year history of Parkinson’s disease (Hoehn and Yahr stage 1). Amantadine hydrochloride was initiated and escalated to 100?mg twice daily with improvement of parkinsonism and initial absence of adverse effects. Approximately four weeks after initiation of amantadine, the patient presented with complaints of oropharyngeal dryness with constant left and intermittent right autophony. Visualization of the tympanic membrane revealed left-sided mobility in association with deep respiration, supporting a diagnosis of Eustachian tube hyperpatency. No additional pathology was identified on flexible laryngoscopic examination. Discontinuation of amantadine resulted in a resolution of symptoms over seven days.
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