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Inhibitory Activity of Ethanol Extract from Artemisia argyi on a Clinical Isolate of Staphylococcus aureus  [PDF]
Fan Zhang, Fan Wang, Liang Xiao, Guoping Sun
Chinese Medicine (CM) , 2014, DOI: 10.4236/cm.2014.54029
Abstract: As a traditional herbal medicine for the treatment of many disorders, Artemisia argyi has shown many valuable bioactivities, but little is known about its effect on Staphylococcus aureus. In this study, the growth, the biofilm formation and the pathogenicity of S. aureus cultivated with or without ethanol extract of A. argyi were tested using microtitre plate assay, Confocal Laser Scanning Microscope (CLSM) system and mice infection assay. Results showed that the growth and the biofilm formation of S. aureus in the test group with ethanol extract of A. argyi were significantly lower than those of the control group without ethanol extract of A. argyi. With CLSM system we could observe that the biofilm structure of the test group had looser and less biomass compared with the control group. After infection of S. aureus, the survival of mice in test group that were given 0.2 mL 100 mg/mL ethanol extracts of A. argyi was higher than the control group. Histopathological analyses showed that the tissue damage of mice in test group was less than that in control group. These results suggested that ethanol extract of A. argyi had inhibitory effect on S. aureus and could protect mice from death induced by S. aureus infection.
Dual Bioactivities of Essential Oil Extracted from the Leaves of Artemisia argyi as an Antimelanogenic versus Antioxidant Agent and Chemical Composition Analysis by GC/MS  [PDF]
Huey-Chun Huang,Hsiao-Fen Wang,Kuang-Hway Yih,Long-Zen Chang,Tsong-Min Chang
International Journal of Molecular Sciences , 2012, DOI: 10.3390/ijms131114679
Abstract: The study was aimed at investigating the antimelanogenic and antioxidant properties of essential oil when extracted from the leaves of Artemisia argyi, then analyzing the chemical composition of the essential oil. The inhibitory effect of the essential oil on melanogenesis was evaluated by a mushroom tyrosinase activity assay and B16F10 melanoma cell model. The antioxidant capacity of the essential oil was assayed by spectrophotometric analysis, and the volatile chemical composition of the essential oil was analyzed with gas chromatography-mass spectrometry (GC/MS). The results revealed that the essential oil significantly inhibits mushroom tyrosinase activity (IC 50 = 19.16 mg/mL), down-regulates B16F10 intracellular tyrosinase activity and decreases the amount of melanin content in a dose-dependent pattern. Furthermore, the essential oil significantly scavenged 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) ABTS radicals, showed an apparent reduction power as compared with metal-ion chelating activities. The chemicals constituents in the essential oil are ether (23.66%), alcohols (16.72%), sesquiterpenes (15.21%), esters (11.78%), monoterpenes (11.63%), ketones (6.09%), aromatic compounds (5.01%), and account for a 90.10% analysis of its chemical composition. It is predicted that eucalyptol and the other constituents, except for alcohols, in the essential oil may contribute to its antioxidant activities. The results indicated that essential oil extracted from A. argyi leaves decreased melanin production in B16F10 cells and showed potent antioxidant activity. The essential oil can thereby be applied as an inhibitor of melanogenesis and could also act as a natural antioxidant in skin care products.
ANTIMICROBIAL ACTIVITY OF ARTEMISIA VULGARIS LINN. (DAMANAKA)  [PDF]
Hiremath S.K.,Kolume D.G.,Muddapur U M.
International Journal of Research in Ayurveda and Pharmacy , 2011,
Abstract: Extracts of Artemisia vulgaris Linn (Damanaka) (Asteraceae) were screened for their in vitro antimicrobial activity agar diffusion method in comparison with standard antibiotics, Gentamycin, Ampicilline, Tetracycline, Ciprofloxin and Ofloxacin. The antimicrobial activity of aqueous, alcoholic, petroleum ether and Benzene extract of leaves of the plant were studied using Escherichia coli and Staphylococcus aureus, as test organisms. All the extracts were effective against all the two micro organisms. The result reveals that the plant extracts have maximum inhibitory activity against Gram negative and Gram positive organism when compared to standard antibiotics. The petroleum ether and benzene extracts of plant have shown significant activity against Escherichia coli and Staphylococcus aureus. Similarly the aqueous and alcohol extract of plant has shown good inhibitory activity of Artemisia vulgaris indicating that the plant can fight these organisms effectively.
艾蒿多糖抑菌活性及稳定性  [PDF]
孙义玄,包怡红
- , 2017, DOI: 10.3969/j.issn.1673-1689.2017.09.015
Abstract: 采用水提法提取艾蒿多糖,用滤纸片法测定多糖的抑菌活性及最低抑菌浓度(MIC),同时研究艾蒿多糖抑菌活性的酸碱稳定性、热稳定性、紫外稳定性。结果表明:艾蒿多糖对3种常见细菌均有一定的抑制效果,对革兰氏阳性细菌如金黄色葡萄球菌和枯草芽孢杆菌抑制作用较强,对革兰氏阴性菌大肠杆菌的抑制作用较弱;艾蒿多糖对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌的最小抑制质量浓度(MIC) 分别为0.625、0.625、1.25 mg/mL;多糖的抑菌活性对温度和紫外照射具有较好稳定性,在不同的pH 值条件下抑菌活性相对不稳定,酸性处理条件下抑菌活性较强,碱性处理条件下抑菌活性较弱。艾蒿多糖具有良好的抑菌活性,可用于天然食品防腐剂的开发利用。
Artemisia argyi polysaccharide extracted by hot water and measurement the antibacterial activity and minimum inhibitory concentration(MIC) of polysaccharides by using the filter paper method,also studied the pH stability,thermal stability,UV stabilizers stability of Artemisia polysaccharide antibacterial activity. The results showed that:Artemisia argyi polysaccharides have a certain inhibitory effect on three common bacteria,and have strong effect on gram-positive bacteria Staphylococcus aureus and Bacillus subtilis,but the effect on gram-negative bacteria E. coli is weak.The MICs of the polysaccharide agaginst Bacillus subtilis,Staphylococcus aureus and Escherichia coli were 0.625、0.625、1.25 mg/mL respectively. The antimicrobial activities of the polysaccharide were stable under the conditions of heat and ultraviolet ray,but were unstable under different pH values,strong in acid and weak in base. Artemisia argyi polysaccharide has good antibacterial activity,it can be used for the development and utilization of natural food preservatives
艾叶等20种中药对禽多杀性巴氏杆菌的体外抗菌活性
Antibacterial activity of crude extract from twenty traditional Chinese medicines like Artemisia argyi against Pasteurella multocida in vitro
 [PDF]

曲径,殷中琼,贾仁勇,彭练慈,康帅,李莉
- , 2015,
Abstract: 通过乙醇回流、水煎煮和超声波等方法提取艾叶等20种中药的有效成分,采用二倍稀释法测定其对禽多杀性巴氏杆菌(C48-1,血清型A∶1)的体外抗菌活性;并研究抗菌活性较强的几味中药的体外联合抑菌效果。结果显示,艾叶、黄连、黄柏、秦皮、地榆、虎杖6种中药提取物对巴氏杆菌的最低抑菌浓度值(MIC)范围在6.25~50 mg/mL;赤芍、何首乌、茵陈、射干、甘草、蒲公英、白鲜皮7种中药提取物的MIC值范围在50~100 mg/mL;金银花、柴胡、板蓝根、夏枯草、穿心莲、知母、苦参7种中药提取物的MIC值大于100 mg/mL。联合抑菌试验结果表明,黄连、黄柏、秦皮、虎杖和艾叶的联合抑菌指数(FICI)≤0.5,黄柏、秦皮、黄连和虎杖的联合抑菌指数(FICI)≥2;秦皮、地榆和黄柏的联合抑菌指数(FICI)≥2。通过以上数据可以看出,黄连、黄柏、地榆、艾叶对巴氏杆菌均具有较好的体外抗菌活性;黄连、黄柏、地榆和艾叶为相加、协同作用;地榆和黄柏为拮抗作用。
Active ingredients of 20 traditional Chinese medicines were extracted by ethanol circumfluence,water decoction,and ultrasonic extraction.Double dilution method is used to determine the antibacterial activity on Pasteurella multocida in vitro.And then the joint bacteriostatic activities of a few traditional Chinese medicines were evaluated.The result showed that the minimal inhibitory concentration (MIC) was in a range of 6.25~50 mg/mL mugwort leaf (Artemisia argyi),Rhizoma coptidis,Cortex phellodendri,Cortex fraxini,Garden burnet,Giant knotweed’s extracts,in range of 50 to 100 mg/mL for Radix paeoniaerubra,Radix-polygonimultiflori,Herba artemisiae capillaris,Rhizomabelamcandae,Licorice root,Dandelion,Cortex dictamni’s extracts,and more than 100 mg/mL for Honeysuckle,Radix bupleuri,Radix isatidis,Prunella vulgaris,Herba andrographitis,Rhizoma anemarrhenae,Radix sophorae flavescentis’s extracts.The results of joint bacteriostatic test showed that the fractional inhibitory concentration index (FICI) of R.coptidis,C.phellodendri,C.fraxini,G.knotweed,and A.argyi were less than or equal to 0.5 and the fractional inhibitory concentration index (FICI) of C.phellodendri,C.fraxini,R.coptidis and G.knotweed,Qin leather,G.burnet and C.phellodendri were more than or equal to 2.In conclusion,R.coptidis,C.phellodendri,G.burnet,and A.argyi had good antimicrobial activities against P.multocida. R.coptidis,C.phellodendri,G.burnet and Mugwort showed additive or synergy action,while Sanguisorba and C.phellodendri showed antagonistic action.
Antimicrobial Activity and Chemical Composition of Essential Oil From the Seeds of Artemisia aucheri Boiss
Gholamreza Asghari,Mohamad Jalali,Ehsan Sadoughi
Jundishapur Journal of Natural Pharmaceutical Products , 2012,
Abstract: Background: Artemisia aerial parts are well known for antimicrobial activities including anti malaria..Objectives: This study was carried out to evaluate the antimicrobial activity and chemical composition of essential oil from the seeds of Artemisia aucheri Boiss (Asteraceae)..Materials and Methods: Essential oil was extracted from the powdered seeds of Artemisia aucheri by hydrodistillation. Antimicrobial activity against five bacterial species was tested using the disc diffusion method, and the chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS)..Results: The essential oil of Artemisia aucheri seed showed activity against Escherichia coli, Staphylococcus aureus, and Listeria monocytogenes. The essential oil constituents identified by GC-MS were as follows: decane, ρ-cymene, 1,8-cineole, linalool, ρ-mentha-8-ol, triene, borneol, lavandulol, bornyl acetate, chrysanthenyl acetate, dehydro aromadenderene, and caryophyllene oxide. Most of these compounds are also found in the aerial parts of Artemisia aucheri..Conclusions: Variation in the compositions of essential oils from Artemisia aucheri, and thus variation in the antimicrobial activity of these oils, may be due to the plant parts used for essential oil prepration.
Chemical Composition and Antibacterial Activity from Essential Oil of Artemisia sieberi Besser subsp. Sieberi in North of Iran  [PDF]
B. Behmanesh,G.A. Heshmati,M. Mazandarani,M.B. Rezaei
Asian Journal of Plant Sciences , 2007,
Abstract: The chemical composition and antibacterial effect of Artemisia siberi essential oil were studied in this research. The composition of essential oil from aerial parts was analyzed by GC/MS and its antibacterial effect were determined by disc diffusion method. Artemisia ketone (48.5%), 1, 8-cineole (19.7%), selin-11-en-4-a-ol (4.6%) and lavandulon (2.8%) were the major constituents of this herbal medicine. Inhibitory zone against Pseudomonas aeroginosa, Staphylococcus aureus and Escherichia coli around discs contained 100 mg mL-1 of Artemisia siberi essential oil were 18, 13 and 12 mm, respectively. Further studies for the determination of anti Pseudomonas infection in animal model are suggested.
Urease Inhibitory Activity of Aerial Parts of Artemisia scoparia: Exploration in an In Vitro Study  [PDF]
Murad Ali Khan,Haroon Khan,Shafiq Ahmad Tariq,Samreen Pervez
Ulcers , 2014, DOI: 10.1155/2014/184736
Abstract: Artimisia scoparia has been used in the treatment of different disorders including ulcers. The current study was therefore designed to investigate the aerial parts of??Artemisia scoparia (crude extract, total sterol and flavonoidal contents, and aqueous fraction) for its urease inhibitory potential. The crude of the plant evoked marked attenuation on urease activity, when tested in various concentrations with IC50 values of 4.06?mg/ml. The inhibitory potential was further augmented in the aqueous fraction (IC50: 2.30?mg/ml) of the plant. When the total sterol and flavonoidal contents were challenged against urease, both showed concentration dependent activity; the latter showed maximum potency with IC50 values of 8.04 and 2.10?mg/ml, respectively. In short, the aerial parts of the plant demonstrated marked antagonism on urease and thus our study validated the traditional use of Artemisia scoparia in the treatment of ulcer. 1. Introduction Urease (urea amidohydrolase) is usually found in different bacteria, fungi, algae, and plants, an enzyme that catalyzes the hydrolysis of urea to ammonia and carbamate, which is the final step of nitrogen metabolism in living organisms [1]. Carbamate rapidly and spontaneously decomposes, yielding a second molecule of ammonia. These reactions may cause significant increase in pH and are responsible for negative effects of urease activity in human health and agriculture [2, 3]. From the medical viewpoint, infections produced by these bacteria such as Helicobacter pylori and Proteus mirabilis usually have a high urease activity. Urease is central to H. pylori metabolism and virulence, is necessary for its colonization of the gastric mucosa, and is a potent immunogen that elicits a vigorous immune response. This enzyme is used for taxonomic identification and for diagnosis and followup after treatment and is a vaccine candidate. Urease represents an interesting model for metalloenzyme studies. Before the discovery of H. pylori, humans were thought to produce “gastric urease.” It is now known that the source of this notable protein is this bacterium, which colonizes the gastric mucosa of humans. It contributes in urinary tract and gastrointestinal infections, probably augmenting the severity of several pathological conditions like peptic ulcers and stomach cancer as in the case of H. pylori. Ureases are also involved in the development of different human and animal pathogenicity of urolithiasis, pyelonephritis, hepatic encephalopathy, hepatic coma, and urinary catheter encrustation [4–6]. Being involved in the
Antimicrobial activity and chemical components of two plants, Artemisia capillaris and Artemisia iwayomogi, used as Korean herbal Injin  [cached]
Kyoung Sun Seo,Hyung Jin Jeong,Kyeong Won Yun
Journal of Ecology and Field Biology , 2010,
Abstract: This study compared the antimicrobial activity and chemical composition of two plants used as “Injin” (Korean herbalmedicine), namely, Injinho (Artemisia capillaris Thunberg) and Haninjin (Artemisia iwayomogi Kitamura). The ethyl acetateand ether fractions of crude methanol extracts from A. capillaris and A. iwayomogi were tested against three grampositivebacteria (Bacillus cereus, Bacillus subtilis, Staphylococcus aureus), two gram-negative bacteria (Escherichia coli,Pseudomonas fluorescens), and a yeast (Saccharomyceus cerevisiae). The antimicrobial activity of the ethyl acetate andether fraction of both plants was strong, but that of A. iwayomogi extracts was higher than that of A. capillaris extract forthe microbes tested. The minimum inhibitory concentration of the ether and ethyl acetate fraction of A. iwayomogi washighest for P. fluorescens and lowest for S. aureus and E. coli. We analyzed the chemical composition of the ethyl acetatefraction of A. capillaris and A. iwayomogi using gas chromatography-mass spectrometry. The main components of A.capillaris and A. iwayomogi were escoparone (86.82%) and scopoletin (20.47%), respectively.
In vitro antibacterial activity of Artemisia annua Linn. growing in India
Gupta Prakash,Dutta B,Pant D,Joshi P
International Journal of Green Pharmacy , 2009,
Abstract: The crude extracts obtained from the aerial parts of Artemisia annua Linn. (Asteraceae) were investigated for their antibacterial activity by using agar well diffusion assays against five Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus pumilus, Bacillus cereus, and Micrococcus luteus) and three Gram-negative bacteria (Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa). Of the various extracts, the methanol extract showed the strongest activity against most bacteria used in this study. The most sensitive organism to the extracts was M. luteus. The minimum inhibitory concentration (MIC) values were determined by the tube dilution method. The results showed that S. aureus required ~0.25 mg/mL of the methanol extract for inhibition. The HPTLC fingerprint of the methanol extract after derivatization with anisaldehyde sulphuric acid reagent showed a maximum number of separated components. TLC bioautography of the methanol extract showed that the area of inhibition around compounds differentiated at R f = 0.32, R f = 0.42, R f = 0.46, R f = 0.77, and R f = 0.87 against S. aureus. This is the first report of the antibacterial activity of A. annua against food-borne bacteria. The results indicated that aerial parts of A. annua might be potential sources of new antibacterial agents.
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