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Optimization of the Protocol for the in Vitro Cultivation of Piper aduncum L.  [PDF]
Samia Torres Silva, Fernanda Ventorim Pacheco, Ivan Caldeira Almeida Alvarenga, José Eduardo Brasil Pereira Pinto, Suzan Kelly Vilela Bertolucci, Cibelly Pereira Ferreira
American Journal of Plant Sciences (AJPS) , 2014, DOI: 10.4236/ajps.2014.523363
Abstract:
Piper aduncum L. (Piperaceae) has great potential for economic exploration because of the proven use of its essential oil in the agriculture and in the human health. A technique that improves its propagation, as the tissue culture, becomes necessary. Some parameters must be determined for the successful cultivation in vitro. Thus, this study aimed to determine the salts concentration of MS medium, temperature, luminosity and light quality for in vitro culture of this species. The following treatments were conducted: 1/4MS, 1/2MS, MS and 2MS; 20, 25, 30 and 35; monochromatic blue, red and white lights and the combination of red and blue, using light emitting diodes (LEDs); luminosities of 17, 37, 48 and 73 μmol·m-2·s-1 obtained with cool white fluorescent lamp. After 35 days, the treatments were analyzed. To determine the salts concentration of MS, the variables were evaluated: number of shoots, leaves and roots, length and width of leaves, length and dry mass of root and shoots and percentage of death by necrosis. To the plantlets under different temperatures, length and dry mass of shoot and root, number of shoots, number, length and width of leaves and survival and rooting percentages were measured. The plantlets that were maintained under different luminosities were evaluated for length of shoot and root, dry mass of leaf and root. To evaluate the growth under the LEDs, the length and dry mass of shoot and root, number of shoots and roots, percentage of rooting and sprouting were assessed. The medium 1/4MS and the medium 1/2MS showed better responses for number and length of root, leaf width and shoot length. The temperature 25 provided the highest number of leaves, length of shoot and root, root dry mass and rooting percentage. The luminosity 73 μmol·m-2·s-1 provided higher values in length of root and dry mass of leaf and root. The red LED provided plantlets with greater growth. Thus, for the in vitro cultivation of Piper aduncum, 1/4MS, environment temperature of 25, light intensity of 73
In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions
Silva, Tatiane Loureiro da;Scherwinski-Pereira, Jonny Everson;
Pesquisa Agropecuária Brasileira , 2011, DOI: 10.1590/S0100-204X2011000400007
Abstract: the objective of this work was to evaluate in vitro storage of piper aduncum and p. hispidinervum under slow-growth conditions. shoots were stored at low temperatures (10, 20 and 25°c), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - aba (0, 0.5, 1.0, 2.0 and 3.0 mg l-1). after six-months of storage, shoots were evaluated for survival and regrowth. low temperature at 20oc was effective for the in vitro conservation of p. aduncum and p. hispidinervum shoots. in vitro cultures maintained at 20oc on ms medium showed 100% survival with slow-growth shoots. the presence of mannitol or aba, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.
Efecto neuroprotector del extracto hidroalcohólico de Piper aduncum “matico” en un modelo in vitro de neurodegeneración The neuroprotective effect of a hydroalcoholic extract of Piper aduncum “matico” in an in vitro model of neurodegeneration  [cached]
César Zaa,Martha Valdivia,álvaro Marcelo
Revista Peruana de Biología , 2013,
Abstract: Durante los procesos neurodegenerativos la función y viabilidad de las neuronas se reduce. En particular, el incremento patológico en la concentración de calcio intracelular, la alteración de la plasticidad sináptica y apoptosis están implicados en la Enfermedad de Alzheimer (EA), paradigma de un proceso neurodegenerativo, relacionado con la pérdida progresiva de las funciones cognitivas. Para evaluar el efecto neuroprotector de Piper aduncum“matico” se indujo el da o con el péptido Aβ a células cultivadas. Igualmente, células hipocam-pales fueron tratadas con el Aβ, y se evaluó viabilidad celular, niveles de caspasa-3 y expresión de receptores NMDA en sinapsis. También se registró el influjo de calcio intracelular en tratamientos con agonista NMDA y P. aduncum. En la evaluación neuroprotectora hay una reducción de un 20,6% de caspasa-3; un aumento del 9,6% por encima del control y una recuperación del 20,86% para las proteínas NR1 y SV2 respectivamente. Además hay una reducción de más del 50% del calcio celular. Estos resultados evidencian efecto neuropro-tector de P. aduncumpara el modelo estudiado. During the neurodegenerative processes there is a reduction of the function and viability of neurons. The pathological increase in the intracellular calcium concentration, the alteration of the synaptic plasticity and the apoptosis are implicated in Alzheimer disease (AD, a paradigm of a neurodegenerative process, related with the progressive loss of cognitive functions. To evaluate neuroprotector effect of Piper aduncumdamage was induced with Aβ1-42 and NMDA to cultured cells. So hippocampal cells were treated with Aβ 1-42 1 uM and cellular viability, levels of caspasa-3 and expression of NMDA receptors in synapses were evaluated. Also, intracellular calcium influxe (sobreestimulation with NMDA) of was registered in treatments with P. aduncum. In the neuroprotector evaluation there is a reduction of 20.6% of caspasa-3; one increase of 9.6% over control and recovery of 20.86% for NR1 and SV2 proteins respectively. Besides, there is a reduction of more than 50% of cellular calcium. These results demonstrate neuroprotector effect of P. aduncumfor the studied model.
Citogenética de Piper hispidinervum e Piper aduncum
Nunes, Juliane Dornellas;Torres, Giovana Augusta;Davide, Lisete Chamma;Salgado, Caio Césio;
Pesquisa Agropecuária Brasileira , 2007, DOI: 10.1590/S0100-204X2007000700019
Abstract: the aim of this work was to detect differences between two species of long pepper by karyotypic analysis. five acessions of piper hispidinervum c. dc. (piperaceae) and piper aduncum l. (piperaceae), belonging to germplasm collection of embrapa acre, were analysed using squashing technique and feulgen staining. the chromosome number observed for both species was 2n = 24 and karyotype presented small metacentric chromossomes, with average length of 1.38 μm in p. hispidinervum and 1.32 μm in p. aduncum. karyotypic descriptions pointed out that the species are not different.
Composition and antifungal activity of essential oils from Piper aduncum, Piper arboreum and Piper tuberculatum
Navickiene, Hosana M. Debonsi;Morandim, Andréia de A.;Alécio, Alberto C.;Regasini, Luís O.;Bergamo, Debora Cristina B.;Telascrea, Marcelo;Cavalheiro, Alberto J.;Lopes, Márcia N.;Bolzani, Vanderlan da S.;Furlan, Maysa;Marques, Márcia O. M.;Young, Maria C. M.;Kato, Massuo J.;
Química Nova , 2006, DOI: 10.1590/S0100-40422006000300012
Abstract: the composition of essential oils from leaves, stems and fruits of piper aduncum, p. arboreum and p. tuberculatum was examined by means of gc-ms and antifungal assay. there was a predominance of monoterpenes in p. aduncum and p. tuberculatum and of sesquiterpenes in p. arboreum. p. aduncum showed the richest essential oil composition, including linalool. the essential oils from fruits of p. aduncum and p. tuberculatum showed the highest antifungal activity with the mic of 10 μg as determined against cladosporium cladosporioides and c. sphaerospermum, respectively. this is the first report of the composition of essential oils from p. tuberculatum.
Composition and antifungal activity of essential oils from Piper aduncum, Piper arboreum and Piper tuberculatum  [cached]
Navickiene Hosana M. Debonsi,Morandim Andréia de A.,Alécio Alberto C.,Regasini Luís O.
Química Nova , 2006,
Abstract: The composition of essential oils from leaves, stems and fruits of Piper aduncum, P. arboreum and P. tuberculatum was examined by means of GC-MS and antifungal assay. There was a predominance of monoterpenes in P. aduncum and P. tuberculatum and of sesquiterpenes in P. arboreum. P. aduncum showed the richest essential oil composition, including linalool. The essential oils from fruits of P. aduncum and P. tuberculatum showed the highest antifungal activity with the MIC of 10 μg as determined against Cladosporium cladosporioides and C. sphaerospermum, respectively. This is the first report of the composition of essential oils from P. tuberculatum.
Luz e temperatura na germina??o de sementes de pimenta longa (Piper hispidinervum) e pimenta-de-macaco (Piper aduncum)
Bergo, Celso Luis;Silva, Rosemeire Carvalho da;Ohlson, Osvaldo de Castro;Biasi, Luiz Antonio;Panobianco, Maristela;
Revista Brasileira de Sementes , 2010, DOI: 10.1590/S0101-31222010000300019
Abstract: the species piper hispidinervum and piper aduncum stand out as producers of essential oils, rich in compounds, such as saffrol and dillapiol, which are of interest to the fragrance and pesticide industries. these species are being domesticated and studies about their seed germination process are scarce and the aim of this study was to develop an efficient protocol for germination tests with which to evaluate seed viability in p. hispidinervum and p. aduncum. the study was carried out in july and august, 2008, when the seeds were submitted to different combinations of temperature and light: 20 oc and 25 oc with diffuse, white light; and 20-30 oc with alternating dark (16 h) and light (8 h) periods, totaling 5 treatments. the treatment with 25 oc and white light gave the best results in terms of higher percentages and germination rates, and therefore, these conditions are recommended for evaluating seed physiological quality of long pepper and monkey pepper.
Volatile Chemical Constituents of Piper aduncum L and Piper gibbilimbum C. DC (Piperaceae) from Papua New Guinea  [PDF]
Topul Rali,Stewart W Wossa,David N Leach,Peter G Waterman
Molecules , 2007, DOI: 10.3390/12030389
Abstract: Exhaustive hydro-distillation of the leaves of Piper aduncum and fruits of Piper gibbilimbum (Piperaceae) afforded colorless and pale orange colored oils in 0.35 and 0.30 % yields, respectively. Detailed chemical analysis by GC/MS indicated the volatile constituents of Piper aduncum to be composed of dill apiole (43.3 %), β-caryophyllene (8.2 %), piperitione (6.7 %) and α-humulene (5.1 %), whilst the oil of P. gibbilimbum is dominated by the gibbilimbols A-D (74.2 %), with the remaining major constituents being the terpenes camphene (13.6 %) and α-pinene (6.5 %).
Toxicidade de óleos essenciais de Piper aduncum e Piper hispidinervum em Sitophilus zeamais
Estrela, Joelma Lima Vidal;Fazolin, Murilo;Catani, Valdomiro;Alécio, Marcio Rodrigues;Lima, Marilene Santos de;
Pesquisa Agropecuária Brasileira , 2006, DOI: 10.1590/S0100-204X2006000200005
Abstract: essential oils of piper aduncum l. and piper hispidinervum c. dc. (piperaceae) were tested against sitophilus zeamais motsch. contact, fumigant toxicity and topical effect were tested. probit analysis was employed in evaluating the lc50 and ld50 response. linear regression analysis and superposition of the bars (means error standard) were used for comparison of means percentage mortality. s. zeamais was more susceptible to the contact toxicity of the p. hispidinervum oil than p. aduncum oil with lc50 values of 0.51 and 2.87 ml cm-2 of the oil, respectively. the mortality rate was nearly 100% at p. hispidinervum oil concentrations of 20 and 30%. with respect to the fumigant action, the weevil was more susceptible to the p. aduncum oil than to p. hispidinervum. a significant difference between oils mortality was observed only at 0.1 and 1.0% concentrations. for topical effect, similar values of the ld50 were obtained for essential oils. however, the mortality was higher with p. aduncum. essential oils of the p. aduncum and p. hispidinervum possess insecticidal effect from s. zeamais, but the responses varied according to concentration of the oils and exposure methods.
Identification of Polyphenols and Anti-Oxidant Capacity of Piper aduncum L.
Piper aduncumMónica Ramos Escudero, D. Fernando Ramos Escudero, Connie M. Remsberg, Jody K. Takemoto, Neal M. Davies, Jaime A. Yá ezPiper aduncum LP. aduncum L
The Open Bioactive Compounds Journal , 2009, DOI: 10.2174/1874847300801010018]
Abstract: Ethanol extracts of Piper aduncum L. leaves from five sub-regions in the tropical Peruvian rainforest were assessed for polyphenol content utilizing liquid chromatography coupled with mass spectrometry with electrospray ionization (LC-MS-ESI). The anti-oxidant capacity was estimated using a DPPH radical scavenging assay. Additionally, the reducing power (capacity to reduce Fe3+ to Fe2+), the capacity to scavenge hydrogen peroxide and the total polyphenol content, were assessed. Analysis led to the identification of gallic acid, catechin, chlorogenic acid, epicatechin, rutin, quercitrin, phloridzin, quercetin, and phloretin, as well as determination of potent anti-oxidant capacities by P. aduncum L.
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