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Research of cultivation and acclimation of activated sludge before biofilm colonization in sequencing batch biofilm reactor (SBBR)

Wan Jinbao,Deng Xiangping,Wang Jingbin,Wu Yongming,

环境工程学报 , 2009,
Abstract: The research is on the biodegradation of the hoggery's wastewater before the hanging of the microbes to domesticate the activated sludge in a sequencing batch biofilm reactor(SBBR).The results show that the removal rates of COD and NH3-N achieved 85% and 74% respectively at the end of the acclimation period.It also analyses some abnormal phenomenons such as sludge bulking,floating and foam problems in the acclimation process and proposes the corresponding measures.
Bacterial Diversity in Sequencing Batch Biofilm Reactor (SBBR) for Landfill Leachate Treatment Using PCR-DGGE

XIAO Yong,YANG Zhao-hui,ZENG Guang-ming,MA Yan-he,LIU You-sheng,WANG Rong-juan,XU Zheng-yong,

环境科学 , 2007,
Abstract: For studying the bacterial diversity and the mechanism of denitrification in sequencing bath biofilm reactor (SBBR) treating landfill leachate to provide microbial evidence for technique improvements, total microbial DNA was extracted from samples which were collected from natural landfill leachate and biofilm of a SBBR that could efficiently remove NH+4-N and COD of high concentration. 16S rDNA fragments were amplified from the total DNA successfully using a pair of universal bacterial 16S rDNA primer, GC341F and 907R, and then were used for denaturing gradient gel electrophoresis (DGGE) analysis. The bands in the gel were analyzed by statistical methods and excided from the gel for sequencing, and the sequences were used for homology analysis and then two phylogenetic trees were constructed using DNAStar software. Results indicated that the bacterial diversity of the biofilm in SBBR and the landfill leachate was abundant, and no obvious change of community structure happened during running in the biofilm, in which most bacteria came from the landfill leachate. There may be three different modes of denitrification in the reactor because several different nitrifying bacteria, denitrifying bacteria and anaerobic ammonia oxidation bacteria coexisted in it. The results provided some valuable references for studying microbiological mechanism of denitrification in SBBR.
Removal of Adsorbable Organic Halides (AOX) from Recycled Pulp and Paper (P&P) Mill Effluent Using Granular Activated Carbon–Sequencing Batch Biofilm Reactor (GAC-SBBR)  [cached]
Abu Bakar Mohamad,Rakmi Abd Rahman,Abdul Amir Hassan Kadhum,Siti Rozaimah Sheikh Abdullah
Modern Applied Science , 2009, DOI: 10.5539/mas.v2n5p37
Abstract: Paper mills generate varieties of pollutants depending upon the type of the pulping process. The wastewaters discharged from these mills have high chemical oxygen demand (COD) and colour, in which indicating high concentrations of recalcitrant organics. This study was conducted using a Granular Activated Carbon – Sequencing Batch Biofilm Reactor (GAC-SBBR) of 3.0 L working volume, operated in an aerobic condition and packed with 200 gL-1 of 2-3 mm granular activated carbon (coconut shells) as a medium for the biofilm growth. For the six of months, the hydraulic retention time (HRT) was set at 36 hours and later it was adjusted to 24 hours in order to evaluate the performance of the system. The treated wastewater samples for these studies were taken from a recycled pulp and paper mill factory in Pahang, Malaysia with 4 different batch characteristics. The adsorbable organic halides (AOX) that had been determined and treated were pentachlorophenol (PCP), 2,3,4,5-tetrachlorophenol (2,3,4,5-TeCP), 2,4,6-trichlorophenol (2,4,6-TCP), 2,4-dichlorophenol (2,4-DCP), 2-chlorophenol (CP) and phenol at various concentration ranges. The Monod growth kinetic parameters for the process specific growth rate coefficient (μh), half saturation coefficient (Ks), endogenous decay coefficient (DH) and Yield coefficient (YH) obtained were 0.0037 hr-1, 65.23 mgL-1, 4x10-5 hr-1 and 0.36 mg/mg, respectively. Analysis of the growth kinetic parameters in GAC-SBBR had deduced that the system was suitable to operate on long biomass retention time (BRT) under anoxic condition. The results also indicated that the biofilm attached onto granular activated carbon (GAC) can substantially remove these recalcitrant organics in the wastewater, within the range of 10 – 100% AOX removal depending on the selected HRTs.
Performance of nitrogen and phosphorus removal from municipal wastewater of different C/N ratios using intelligent controlled systems sequencing batch biofilm reactor ( SBBR)

Jin Yun-Xiao,Feng Chuan-Ping,Ding Da-Hu,Hao Chun-Bo,Song Lin,
Jin YX
,Feng CP,Ding DH,Hao CB,Song L

环境科学 , 2011,
Abstract: The laboratory-scale experiments were conducted to treat synthetic municipal wastewater at different C/N ratios, using a developed intelligent controller sequencing batch biofilm reactor (SBBR), which formed alternative aerobic-anoxic environment. The performance of nitrogen and phosphorus removal was investigated under different conditions (T = 25 degrees C +/- 1 degrees C, V(aeration) = 150 L/h, COD = 300 mg/L, TP = 5 mg/L, TN = 30 mg/L, t(operation) = 15 d; TN = 60 mg/L, t(operation) = 20 d; TN = 90 mg/L, t(operation) = 20 d). The average removal efficiency of COD reaches 85.2%, 91.1% and 97.7%, the average removal efficiency of TP achieves 94.1%, 95.9%, 96.7% ,the average removal efficiency of NH4(+) -N reaches 95.8%, 99.2%, 80.0%, and the average removal efficiency of TN are 90.7%, 81.1%, 58.3%, respectively. With the decrease of C/N ratios, the removal efficiency of N decreases significantly, while the removal efficiency of COD and TP increase slightly. In intelligent controlled SBBR, simultaneous nitrification and denitrification and shortened simultaneous nitrification and denitrification occur, meanwhile, the denitrification of the system strengthens with the increasing of C/N ratios. After the influent finished, intelligent controlled SBBR starts to operate in aerobic-anoxic environment. The efficiency of phosphorus removal is high, moreover, the theory of phosphorus removal is different from the conventional theory of release anaerobic and uptake aerobic phosphorus.
Approaches of Biological Nitrogen Removal in a Single Sequencing Batch Biofilm Reactor

XU Zheng-yong,YANG Zhao-hui,ZENG Guang-ming,WANG Rong-juan,XIAO Yong,XU Zhen,

环境科学 , 2007,
Abstract: The conventional microorganism techniques and the molecule biological techniques such as PCR and DGGE were utilized to study the approaches of biological nitrogen removal in a single sequencing batch biofilm reactor (SBBR). The main approach of biological nitrogen removal, no less than 65% of the total NH+4-N was removed in this approach, was composed of partial nitrification, anaerobic ammonium oxidation and denitrification.The second approach included twain processes such as partial nitrification and denitrification, and the third one was conventional nitrogen removal process (nitrification and denitrification).All approaches accomplished simultaneously or asynchronously. When the approach occurred in the simultaneous way, it performed as nitrogen-loss.The accomplisher of asynchronous way depended on the microbe's different activity between aerated phase and anaerobic phase.First, ammonia was converted to nitrite in the aerated phase when the nitrite bacteria performed high activity, and then anaerobic phase was operated in turn when the denitrifying bacteria and the anammox bacteria eliminated the nitrogen form gases.
Experimental study on treatment of meat product plant wastewater by sequencing batch biofilm reactor

Li Ping,Yuan Heqing,

环境工程学报 , 2008,
Abstract: 在温度为26℃,pH保持在6.8~7.3的条件下,启动序批式生物膜反应器(SBBR)反应器,并在对曝气时间和DO浓度进行优化调整的基础上,对SBBR工艺处理肉类制品厂废水进行研究。SBBR工艺处理肉类制品厂废水的优化参数为:曝气时间8 h,DO浓度为2 mg/L。在此综合优化参数下稳定运行20 d,COD和BOD5的平均去除率分别达到了91.5%和92.5%,氨氮的平均去除率为90.6%,最高达到了92.3%,SS的去除率也保持在90%以上。优化参数条件下SBBR工艺针对肉类制品厂废水的处理效果比较理想。
Treatment of mustard tuber wastewater by anaerobic sequencing batch biofilm reactor-two-stage sequencing batch biofilm reactor-chemical-dephosphorization process

Chai Hongxiang,Li Xiaopin,Zhou Jian,Chen Yao,Long Tengrui,

环境工程学报 , 2010,
Abstract: 针对榨菜腌制废水高盐高氮磷高有机物浓度的特征,提出“厌氧序批式生物膜反应器(ASBBR)-二级序批式生物膜反应器(SBBR)-化学除磷”组合处理工艺,在前期对组合工艺中单元工艺的关键工况参数研究的基础上,考察组合工艺的处理效能。实验结果表明,采用该组合工艺,可使进水COD、NH+4-N、TN及PO3-4-P分别为10 000、345、550和38.5 mg/L的榨菜腌制废水,处理出水COD、NH+4-N、TN及PO3-4-P分别达到93.6、12.3、18和0.1 mg/L,去除率分别为99.1%、96.4%、96.7%和99.9%,出水达到污水综合排放一级标准。
Mechanism studies on the nitrogen removal when treating the ammonia nitrogen rich leachate by sequencing batch biofilm reactor (SBBR)

XU Zhengyong,YANG Zhaohui,ZENG Guangming,XIAO Yong,DENG Jiuhua,

环境科学学报 , 2006,
Abstract: 采用自主设计的SBBR反应器处理氨氮浓度含量较高的垃圾填埋场渗滤液并对其脱氮机理进行分析.在保持(32±0.4)℃的环境温度下,经过58 d的驯化和33 d的稳定,SBBR反应器的脱氮效率最高达到95%.实验结果表明,高频间歇式曝气方式在抑制了硝酸细菌的活性的同时也消除了亚硝酸盐浓度和pH大幅波动对亚硝酸细菌和厌氧氨氧化细菌活性的影响;在曝气阶段,溶解氧浓度被控制在1.2~1.4 mg·L-1,亚硝酸细菌成为主体细菌,亚硝酸盐积累;在缺氧阶段,随着溶解氧浓度迅速降低,厌氧氨氧化细菌成为优势菌种,曝气阶段积累的亚硝酸盐与氨氮同时被去除.
Effect of aeration flow on microbial characteristics and nitrogen transformation of shortcut nitrification processing in a sequencing batch biofilm reactor

DING Wenchuan,WU Dan,ZENG Xiaolan,LUO Ting,LI Qiao,XU Zhou,

环境科学学报 , 2012,
Abstract: A laboratory-scale sequencing batch biofilm reactor (SBBR) was used to investigate characteristics of biofacies and correlative performance of shortcut nitrification process under different aeration flows of 7.2 L·h-1, 12.0 L·h-1and 15.6 L·h-1 (corresponding with averaged dissolved oxygen concentration of around 0.5 mg·L-1, 0.8 mg·L-1 and 1.2 mg·L-1). The experimental results showed that total biomass of biofilm in SBBR substantially reduced with decreased dissolved oxygen concentration during the aerobic phase. The ammonia-oxidizing bacteria (AOB) became the dominant population in the system at low dissolved oxygen concentration. Both number and biological activity of AOB were relatively higher than that of nitrite-oxidizing bacteria (NOB) that resulted in nitrite accumulation. The dissolved oxygen demand during the whole cyclic treatment was not uniform. At the early stage of the aeration phase, dissolved oxygen was preferentially consumed by heterotrophic bacteria for COD degradation. Then it was utilized for ammonia oxidation. Based on the results, we proposed Multi-section Declining Aeration (MSDA) as an improved aeration mode for the shortcut nitrification processing. In MSDA mode, initial high aeration flow was supplied to enhance COD degradation. Thereafter, aeration flow was switched down to maintain low dissolved oxygen concentration which could facilitate nitrosification for nitrite accumulation and optimize energy consumption.
Study of adding polyurethane foam as micro-organisms immobilized carriers in sequencing batch biofilm reactor to remove nitrogen and phosphorus

Li Yafeng,Yao Jingbo,Hao Ying,Li Jin,Wang Xun,Qin Yamin,

环境工程学报 , 2011,
Abstract: 研究投加聚氨酯泡沫微生物固定化载体的SBBR脱氮除磷效果及机理。在SBR中填充聚氨酯泡沫微生物固定化载体,形成序批式生物膜反应器(SBBR)。SBBR以实际生活污水为处理对象,在A/O运行工况下对微生物进行培养驯化,并在SBBR运行稳定时研究其脱氮除磷效果。研究结果表明,SBBR中的聚氨酯泡沫微生物固定化载体在空间上形成了好氧/缺氧/厌氧微环境;SBBR不仅对有机物有较好的处理效果,而且获得较好的同时硝化反硝化(SND)效果,COD、NH+4-N、TN和TP的平均去除率分别达到84.23%、96.94%、68.63%和91.38%。投加聚氨酯泡沫微生物固定化载体的SBBR对生活污水具有理想的处理效果,处理后出水除TP外均达到达到了国家《城镇污水处理厂污染物排放标准》(GB18918-2002)一级A标准,TP接近一级A标准。
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