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PREVALENCE AND IDENTIFICATION OF VIBRIO SPP. ISOLATED ON AQUACULTURED GILTHEAD SEA BREAM
C. Scarano,A. Dalmasso,C. Spanu,V. Spanu
Italian Journal of Food Safety , 2011, DOI: 10.4081/ijfs.2011.1s.115
Abstract: The aim of the study was to investigate the prevalence of Vibrio spp isolated from gilthead sea bream (Sparus aurata) farmed on sea cages and to identify and characterize the pathogen by molecular techniques. Eighty fish were collected from two hatcheries located on the North-Est Sardinian Mediterranean coast, and microbiological analysis were performed on different body parts such as skin, gills, muscle and intestinal tract. Subsequently 100 pure colonies with typical morphology and phenotypic characteristics were selected and submitted to the molecular identification. The analysis on the prevalence of Vibrio spp showed the effect of the hatchery rearing system (P<0.001), of the date of sampling (P<0.001), and of the body part (P<0.001). All the strains selected were confirmed to be members of the genus Vibrio spp by the molecular method/techinique/identification, whereas the rpoA gene sequence analyses allowed to identify 89 strains belonging to the species Vibrio harveyi, 6 to V. diabolicus, 2 to V. parahaemolyticus and 1 to V. mediterranei.
Characterization of Streptomyces spp. Isolated from the Sea Surface Microlayer in the Trondheim Fjord, Norway  [PDF]
Sigrid Hakv?g,Espen Fj?rvik,Kjell D. Josefsen,Elena Ian,Trond E. Ellingsen,Sergey B. Zotchev
Marine Drugs , 2008, DOI: 10.3390/md6040620
Abstract: The water surface microlayer is still poorly explored, although it has been shown to contain a high density of metabolically active bacteria, often called bacterioneuston. Actinomycetes from the surface microlayer in the Trondheim fjord, Norway, have been isolated and characterized. A total of 217 isolates from two separate samples morphologically resembling the genus Streptomyces have been further investigated in this study. Antimicrobial assays showed that about 80% of the isolates exhibited antagonistic activity against nonfilamentous fungus, Gram-negative, and Gram-positive bacteria. Based on the macroscopic analyses and inhibition patterns from the antimicrobial assays, the sub-grouping of isolates was performed. Partial 16S rDNAs from the candidates from each subgroup were sequenced and phylogenetic analysis performed. 7 isolates with identical 16S rDNA sequences were further studied for the presence of PKS type I genes. Sequencing and phylogenetic analysis of the PKS gene fragments revealed that horizontal gene transfer between closely related species might have taken place. Identification of unique PKS genes in these isolates implies that dereplication can not be performed based solely on the 16S rDNA sequences. The results obtained in this study suggest that streptomycetes from the neuston population may be an interesting source for discovery of new antimicrobial agents.
Characterization of Vibrio Alginolyticus Trh Positive From Mediterranean Environment of Tamouda Bay (Morocco)
World Environment , 2012, DOI: 10.5923/j.env.20120204.04
Abstract: Vibrio alginolyticus is a halophilic Vibrio and is considered the most frequent species living freely in water and sediment and can survive in seawater even under starvation conditions while maintaining its virulence. Our objective in this study is to investigate the existence of virulence genes in Vibrio alginolyticus in Tamouda bay (Morocco). A total of 588 samples were collected during the study and analyzed. The study of cultural biochemical and molecular characteristics of strains showed an incidence of 70.2% of Vibrio alginolyticus. Among 412 strains of Vibrio alginolyticus identified eleven (2.7%) were urease and Kanagawa Phenomenon (KP) positive. To study the presence of the gene for virulence genes in ten strains of Vibrio alginolyticus urease positive and KP positive, we used the polymerase chain reaction (PCR). The results revealed that 70% of the strains have the trh gene (250 bp) but all strains are tdh negative. This is the first report who demonstrated the presence of V. alginolyticus KP positive and trh gene in Morocco. These findings indicate the potential sanitary risk associated with the presence of Vibrio alginolyticus KP positive and the implications of the trh gene in plankton, sediment, sea water and shellfish as a pathogen of food poisonning.
Vibrio spp. ISOLATED FROM SHRIMPS AND WATER FROM A MARINE FARM IN PERNAMBUCO, BRAZIL Víbrios en el agua y en las gambas del mar (Litopenaeus vannamei, Boone, 1931) cultivado en Pernambuco Vibrio spp. ISOLADOS DE CAMAR O E áGUA DE CULTIVO DE FAZENDA MARINHA EM PERNAMBUCO  [cached]
Emiko Shinozaki Mendes,Simone Francisca Lira,Lílian Maria Nery de Barros Góes,Joanna Dourado
Ciência Animal Brasileira , 2009,
Abstract: Water and shrimp samples were collected monthly, during all cultivation phases, in three located farms at Pernambuco coast, on winter and summer, for Vibrio spp. quantification and identification. The counting’s were correlated, through mathematical models (P<0.05), with the variables season, water biochemical parameters, wet mount, histopathology exam, toxins presence and handling techniques used. Just the variable cultivation time interfered at total counting of Vibrio spp. in all samples were obtained countings that varied 0.1x 10 to 6.2 x 103 UFC/mL in water, of 7.0 x 10 to 8.2 x 105 UFC/g in powder-larva, of 1.1 x 10 to 1.1 x 105 UFC/mL in hemolymph and of 2.5 x 102 to 1.1 x 106UFC/g in hepatopancreas. The species V. mediterranei (1%), V. mimicus (1.25%), V. fischeri (4.25%), V. cincinnatiensis (4.25%), V. metschnikovii (4.25%), V. proteolyticus (5.5%), V. harveyi (5.5%), V. hollisae (5.5%), V. carchariae (7%), V. vulnificus (8.5%), V. damsela (8.5%), V. parahaemolyticus (13%), V. fluvialis (15%), V. anguillarum (16.5%) were identified. It is concluded that the Vibrionaceae load increases proportionally with cultivation time, due to the organic matter increment, what can turn the susceptible animals to the infection for vibrios. KEY WORD: Vibrionaceae, Vibrio spp., shrimp and pond water. El agua y muestras de la gamba eran la publicación mensual reunido, durante todas las fases del cultivo, en tres granjas localizadas a la costa de Pernambuco, en invierno y verano, para la cuantificación e identificación el de Vibrio spp.. El contando fueron puestos en correlación, a través de los modelos matemáticos (P <0,05), con la estación de las variables, agua que los parámetros bioquímicos, monta a mojada, examen del histopatologia, presencia de las toxinas y técnicas manejando usaron. Simplemente el tiempo del cultivo inconstante interfirió a total que cuenta de Vibrio spp. en todas las muestras se obtuvo contando que el 0,1x variado 10 a 6,2 x 103 UFC/mL en el agua, de 7,0 x 10 a 8,2 x 105 UFC/g en la polvo-larva, de 1,1 x 10 a 1,1 x 105 UFC/mL en el hemolinfa y de 2,5 x 102 a 1,1 x 106UFC/g en el hepatopancreas. Las especies el V anguillarum, V. carchariae, V. harveyi, V. mediterranei, V. hollisae, V. fluvialis, V. vulnificus, V. damsela, V. proteolyticus, V. parahaemolyticus, V. fischeri, V. cincinnatiensis, V. mimicus y V. metschnikovii que se identificaron. Se concluye que los vibrionaceos cargan aumenta proporcionalmente con tiempo del cultivo, debido al incremento de la materia orgánico, lo que puede volverse los animales susceptibles
Vibrio spp. and Salmonella spp., presence and susceptibility in crabs Ucides cordatus
Vieira, Regine H.S.F.;Lima, Elenice Araújo de;Sousa, Dannielle Batista Rolim;Reis, Eliane Falavina dos;Costa, Renata Garcia;Rodrigues, Dália dos Prazeres;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2004, DOI: 10.1590/S0036-46652004000400001
Abstract: the presence of vibrio spp. and salmonella spp. in crabs marketed at the bezerra de menezes ave., fortaleza, state of ceará, brazil, was assessed between february and may, 2003. the number of individuals sampled in each one of the fifteen weekly samplings ranged between four and eight. seven strains of salmonella, from four different samplings, were identified, being five of them identified as serotype s. senftenberg and two as s. poona. all strains of salmonella were sensitive to the tested anti-microbial drugs, with the exception of tetracycline and nalidixic acid, for which an intermediary sensibility was found. the mpn's for vibrio ranged between 110/g and 110,000/g. of the forty five vibrio strains isolated from the crab samples, only 10 were identified up to the species level: two v. alginolyticus and eight v. parahaemolyticus. bacteria belonging to the enterobacteriaceae and pseudomonaceae families were also identified, namely escherichia coli, klebsiella pneumoniae, enterobacter cloacae, pantoea agglomerans and pseudomonas aeruginosa. the proper cooking of the animals is recommended in order to avoid problems for the consumers of this crustacean.
Vibrio spp. isolados de mamíferos marinhos capturados na regi?o litoranea do Sudeste ao Sul do Brasil
Pereira, Christiane S.;Amorim, Simone D.;Santos, André F.M.;Siciliano, Salvatore;Moreno, Ignácio M.B.;Ott, Paulo H.;Rodrigues, Dalia P.;
Pesquisa Veterinária Brasileira , 2007, DOI: 10.1590/S0100-736X2007000200005
Abstract: in the present investigation was evaluated the incidence of vibrio spp. from superficial lesions at marine mammals beached or captured by fishing net in the southwestern (rj) and southern (rs) coastal regions of brazil. one hundred and ninety eight swabs were collected by deensp, gemars and ceclimar institutes and sent to labent/ioc/fiocruz where the samples were submitted to enrichment in alkaline peptone water (apw) added with 1% and 3% of sodium chloride (nacl) incubated at 37oc for 18-24 hours. after the samples were streaked onto thiossulfate citrate bile sucrose agar (tcbs), the suspected colonies were submitted to biochemical characterization. the results showed 108 strains, and vibrio alginolyticus, v. parahaemolyticus, v. vulnificus and v. fluvialis were the main pathogens isolated. these results appoint the importance of surveillance and microbiological monitoring accomplishment and reinforcement of environmental protective programs applied to marine mammals endangered with extinction.
Prevalence and diversity of Babesia spp. in questing Ixodes ricinus ticks from Norway
?ines ?ivind,Radzijevskaja Jana,Paulauskas Algimantas,Rosef Olav
Parasites & Vectors , 2012, DOI: 10.1186/1756-3305-5-156
Abstract: Background Ixodes ricinus ticks transmit Babesia species to vertebrate hosts. Using molecular tools we were able to detect the presence of this piroplasmid in its vector. The aims of this study were to investigate the prevalence and identity of Babesia species in questing ticks collected in various areas of Norway. Methods DNA from questing l. ricinus ticks were examined with a realtime PCR for the presence of Babesia. Positive samples of tick DNA were identified to species using PCR, and sequence analysis. Results From a total of 1908 questing l. ricinus ticks, 17 (0.9%) indicated the presence of Babesia spp. after realtime-PCR screening. Ixodes ricinus harbouring Babesia spp. was detected in 9 out of 22 localities. Further molecular analyses of DNA from these positive ticks indicate the presence of Babesia venatorum, B. divergens, B. capreoli and a currently undescribed Babesia in Norwegian ticks. The most prevalent was B. venatorum found in 71% of the positive ticks. Conclusions A total of 17 out of 1908 (0.9%) ticks were positive for Babesia. Our data confirm that there are several Babesia species in ticks in Norway. Babesia venatorum was the most prevalent. This species has a zoonotic potential and may cause human babesiosis following a tick bite.
Characterization of Vibrio Parahaemolyticus isolated from oysters and mussels in S?o Paulo, Brazil
Rojas, Martha Virginia Ribeiro;Matté, Maria Helena;Dropa, Milena;Silva, Miriam Lopes Da;Matté, Glavur Rogério;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2011, DOI: 10.1590/S0036-46652011000400005
Abstract: vibrio parahaemolyticus is a marine bacterium, responsible for gastroenteritis in humans. most of the clinical isolates produce thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) encoded by tdh and trh genes respectively. in this study, twenty-three v. parahaemolyticus, previously isolated from oysters and mussels were analyzed by pcr using specific primers for the 16s rrna and virulence genes (tdh, trh and tlh) and for resistance to different classes of antibiotics and pfge. nineteen isolates were confirmed by pcr as v. parahaemolyticus. the tlh gene was present in 100% of isolates, the tdh gene was identified in two (10.5%) isolates, whereas the gene trh was not detected. each isolate was resistant to at least one of the nine antimicrobials tested. additionally, all isolates possessed the blatem-116 gene. the presence of this gene in v. parahaemolyticus indicates the possibility of spreading this gene in the environment. atypical strains of v. parahaemolyticus were also detected in this study.
Conventional PCR assays for the detection of pathogenic Vibrio spp. in shrimp aquaculture in the Philippines
Christopher M. A. Caipang,Mary Paz N. Aguana
Aquaculture, Aquarium, Conservation & Legislation , 2011,
Abstract: Shrimp culture is considered as one of the major industries in most aquaculture-producingcountries in Asia. This industry has grown steadily over the years, but its gradual decline is due toseveral disease outbreaks of either bacterial or viral in origin. Luminous vibriosis, which is caused mainlyby Vibrio harveyi has been implicated for mass mortalities in shrimp aquaculture in the Philippines. Inaddition, another pathogen, V. cambellii also causes vibriosis in shrimp, but this bacterium does notexhibit luminescence. The use of molecular methods particularly the polymerase chain reaction (PCR),facilitated early detection of the causative agent; thus, control measures have been undertaken toensure efficient management strategies, thus preventing massive mortalities of the cultured stock. Thispaper reviews the different PCR protocols that have been developed for early detection of pathogenicVibrio spp. in Philippine shrimp aquaculture. These PCR protocols were developed by targeting specificgenes of the pathogen in order to ensure accurate diagnosis of the disease. In addition, the use of directcolony PCR, multiplex PCR and co-amplification together with another viral pathogen is also discussed.
Isolamento de Pasteurella spp. e Vibrio spp. em robalos (Dicentrarchus labrax): susceptibilidade a diferentes grupos de antibióticos
Saavedra, M.J.;Brito, R.D.;Sousa, M.;Alves, A.;Rema, P.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2004, DOI: 10.1590/S0102-09352004000200022
Abstract: the most frequent infectious diseases that affect fish are those of bacterial origin. in european sea bass fish farms (dicentrarchus labrax) are included vibrio spp., pasteurella piscicida and myxobacter spp. in addition to these, it is also possible to find, although lesser frequently, other pathogenic agents such as pseudomonas spp., aeromonas spp., staphylococcus epidermis, streptococcus spp. and enterobacter spp. the presence of these micro-organisms in fish farms contributes for a significant decrease in fish production and subsequent loss of profitability in these aquaculture units. the use of antibiotics may therefore be necessary as a prophylactic measure although their systematic utilization leads to the development of strains of antibiotic resistant bacteria. bearing that in mind, a survey was conducted on the susceptibility of isolated strains of bacteria found in juvenile european sea bass. it was concluded that chloranphenicol and tetracycline are two important antibiotic alternatives for therapy against isolated bacterial agents.
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