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ANTIOXIDANT ACTIVITY OF TAGETES ERECTA ESSENTIAL OIL
PéREZ GUTIéRREZ,ROSA MARTHA; HERNáNDEZ LUNA,HELIODORO; HERNáNDEZ GARRIDO,SERGIO;
Journal of the Chilean Chemical Society , 2006, DOI: 10.4067/S0717-97072006000200010
Abstract: the essential oil from flowers of tagetes erecta was evaluated for antioxidant activity in vitro using diphenyl-1-picrylhydrazyl (dpph), thiocyanate, b-carotene bleaching, free radical scavenging activity and oxidation ofdeoxyribose assay. the gc-ms analysis of the oil has resulted in the identification of 18 components; b-caryophyllene, limonene, methyleugenol, (e)-ocimene, piperetone, piperitenone and ?-terpinolene were the main components
ANTIOXIDANT ACTIVITY OF TAGETES ERECTA ESSENTIAL OIL  [cached]
ROSA MARTHA PéREZ GUTIéRREZ,HELIODORO HERNáNDEZ LUNA,SERGIO HERNáNDEZ GARRIDO
Journal of the Chilean Chemical Society , 2006,
Abstract: The essential oil from flowers of Tagetes erecta was evaluated for antioxidant activity in vitro using diphenyl-1-picrylhydrazyl (DPPH), thiocyanate, b-carotene bleaching, free radical scavenging activity and oxidation of deoxyribose assay. The GC-MS analysis of the oil has resulted in the identification of 18 components; b-caryophyllene, limonene, methyleugenol, (E)-ocimene, piperetone, piperitenone and ?-terpinolene were the main components
In vitro schistosomicidal effects of the essential oil of Tagetes erecta
Tonuci, Ligia R. S.;Melo, Nathalya I. de;Dias, Herbert J.;Wakabayashi, Kamila A. L.;Aguiar, Gabriela P.;Aguiar, Daniela P.;Mantovani, André L. L.;Ramos, Rafael C.;Groppo, Milton;Rodrigues, Vanderlei;Veneziani, Rodrigo C. S.;Cunha, Wilson R.;Silva Filho, Ademar A. da;Magalh?es, Lizandra G.;Crotti, Ant?nio E. M.;
Revista Brasileira de Farmacognosia , 2012, DOI: 10.1590/S0102-695X2011005000202
Abstract: the in vitro schistosomicidal effects of the essential oil obtained from tagetes erecta l. asteraceae, leaves (te-eo) collected in brazil against schistosoma mansoni worms are reported in this paper. the oil caused a significant decrease in the motor activity at 50 μg/ml as minimal concentration after 24 h. this oil also caused death of all the parasites and the separation of coupled pairs into individual male and female at 100 μg/ml after 24 h. the viability of adult worm groups treated with the te-eo at 100 μg/ml was similar to that of groups treated with praziquantel (positive control). in addition, the oil promoted the inhibition of eggs development at all the tested concentrations. these data indicate that the te-eo could be considered as a promising source for the development of new schistosomicidal agents.
In vitro schistosomicidal effects of the essential oil of Tagetes erecta  [cached]
Ligia R. S. Tonuci,Nathalya I. de Melo,Herbert J. Dias,Kamila A. L. Wakabayashi
Revista Brasileira de Farmacognosia , 2012,
Abstract: The in vitro schistosomicidal effects of the essential oil obtained from Tagetes erecta L. Asteraceae, leaves (TE-EO) collected in Brazil against Schistosoma mansoni worms are reported in this paper. The oil caused a significant decrease in the motor activity at 50 μg/mL as minimal concentration after 24 h. This oil also caused death of all the parasites and the separation of coupled pairs into individual male and female at 100 μg/mL after 24 h. The viability of adult worm groups treated with the TE-EO at 100 μg/mL was similar to that of groups treated with praziquantel (positive control). In addition, the oil promoted the inhibition of eggs development at all the tested concentrations. These data indicate that the TE-EO could be considered as a promising source for the development of new schistosomicidal agents.
Chemical composition of essential oil from tagetes minuta L. leaves and flowers
Chamorro,Ester R; Ballerini,Griselda; Sequeira,Alfredo F; Velasco,Gustavo A; Zalazar,María F;
The Journal of Argentine Chemical Society , 2008,
Abstract: the essential oil composition of wild tagetes minuta l., collected from different locations in the province of chaco (argentine) was studied. essential oils of flowers and leaves of bloomed and non-bloomed plants were analyzed by means of gas ghromatography and gas chromatography-mass spectrometry. six main components were identified. essential oil from non-bloomed plant leaves contained mainly dihydrotagetone, while the essential oil from bloomed plants leaves as well as from flowers, were rich in b-ocimene and tagetenone. the essential oil composition of tagetes minuta l. varies according to the different parts of the plant and its growth stage but does not differ with respect to the origin of the material.
Actividad fumigante de aceites esenciales de Schinus molle (Anacardiaceae) y Tagetes terniflora (Asteraceae) sobre adultos de Pediculus humanus capitis (Insecta; Anoplura; Pediculidae)  [cached]
María M. GUTIéRREZ,Natalia STEFANAZZI,Jorge WERDIN GONZáLEZ,Verónica BENZI
Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas , 2009,
Abstract: Los aceites esenciales de hojas se aislaron por hidrodestilación. Los bioensayos se realizaron a 28 ± 2 oC, 65% ± 5 H.R y en oscuridad. Se utilizaron cajas de Petri de vidrio (5cm de diámetro), en sus bases se colocaron 50 mL de los aceites puros. La caja se cubrió con una tapa plástica perforada que posee un tejido de hilo. Sobre este se liberaron 10 insectos protegidos por una segunda caja con diámetro equivalente. El conjunto de cajas se cerró con cinta adhesiva. Se realizaron tres réplicas por tratamiento. Como control se utilizaron cajas sin aceites. El porcentaje de volteo se evaluó cada 5 minutos durante una hora. Se calculó el TV50 por un análisis Probit. El TV50 fue 12,75 min para el aceite de Schinus molle y para el de Tagetes terniflora de 23,44 min. Ambos aceites podrían ser utilizados para el control de Pediculus humanus capitis.
ANTIMICROBIAL ACTIVITY OF EXTRACTS, ESSENTIAL OIL AND METABOLITES OBTAINED FROM TAGETES MENDOCINA
LIMA,BEATRIZ; AGüERO,MARíA BELéN; ZYGADLO,JULIO; TAPIA,ALEJANDRO; SOLIS,CARLOS; ROJAS DE ARIAS,ANTONIETA; YALUFF,GLORIA; ZACCHINO,SUSANA; EGLY FERESIN,GABRIELA; SCHMEDA-HIRSCHMANN,GUILLERMO;
Journal of the Chilean Chemical Society , 2009, DOI: 10.4067/S0717-97072009000100016
Abstract: extracts from the argentinean herb tagetes mendocina phil. (asteraceae) were tested for antimicrobial activity against fungi, bacteria and protozoa. the different extracts (pe, dcm, meoh and etoac) and the essential oil displayed activity mainly against dermatophytes, a group of fungi that produces skin infections. the bioassay-guided fractionation of pe, dcm and etoac extracts led to the isolation of eight compounds including two thiophenes, two acetophenone derivatives, dihydrorosefuran and three flavonoids. itie thiophenes 5-(4-hydroxy- l-butynyl)-2,2'-bithienyl (bbtoh) (1) and its acetate (bbtoac) (2) proved to be highly active against the dermatophytes microsporum gypseum, trichophyton rubrum and t. mentagrophytes with mic values of 3.12, 3.12 and 6.25 μg/ml for compound 1 and 25, 3.12 and 6.25 μg/mlfor compound 2, respectively. in turn, the essential oil displayed a weak to modérate antifungal effect against m. gypseum with mic = 250 μg/ml. itie essential oil was examined by gc and gc-ms and 21 constituents (97.3%) were identified. (e) p-ocimene, (z)-tagetone, (ii)-tagetone, (z)-ocimenone, á-pinene and (e)-ocimenone were the major components. in addition, thiophenes 1 and 2 and 2-hydroxyacetophenone 3 showed antiprotozoal activity against leishmania amazonensis, l. brasiliensis andi. infantum promastigotes with 100% lysis at 100 μg/ml.
ANTIMICROBIAL ACTIVITY OF EXTRACTS, ESSENTIAL OIL AND METABOLITES OBTAINED FROM TAGETES MENDOCINA  [cached]
BEATRIZ LIMA,MARíA BELéN AGüERO,JULIO ZYGADLO,ALEJANDRO TAPIA
Journal of the Chilean Chemical Society , 2009,
Abstract: Extracts from the Argentinean herb Tagetes mendocina Phil. (Asteraceae) were tested for antimicrobial activity against fungi, bacteria and protozoa. The different extracts (PE, DCM, MeOH and EtOAc) and the essential oil displayed activity mainly against dermatophytes, a group of fungi that produces skin infections. The bioassay-guided fractionation of PE, DCM and EtOAc extracts led to the isolation of eight compounds including two thiophenes, two acetophenone derivatives, dihydrorosefuran and three flavonoids. ITie thiophenes 5-(4-hydroxy- l-butynyl)-2,2'-bithienyl (BBTOH) (1) and its acetate (BBTOAc) (2) proved to be highly active against the dermatophytes Microsporum gypseum, Trichophyton rubrum and T. mentagrophytes with MIC values of 3.12, 3.12 and 6.25 μg/mL for compound 1 and 25, 3.12 and 6.25 μg/mLfor compound 2, respectively. In turn, the essential oil displayed a weak to modérate antifungal effect against M. gypseum with MIC = 250 μg/mL. ITie essential oil was examined by GC and GC-MS and 21 constituents (97.3%) were identified. (E) p-ocimene, (Z)-tagetone, (ii)-tagetone, (Z)-ocimenone, á-pinene and (E)-ocimenone were the major components. In addition, thiophenes 1 and 2 and 2-hydroxyacetophenone 3 showed antiprotozoal activity against Leishmania amazonensis, L. brasiliensis andi. infantum promastigotes with 100% lysis at 100 μg/mL.
Effect of Administration of the Essential Oil from Tagetes minuta L. Leaves in Wistar Rats  [PDF]
O.O. Odeyemi,M.T. Yakubu,P.J. Masika,A.J. Afolayan
Journal of Biological Sciences , 2008,
Abstract: The effects of the essential oil from Tagetes minuta leaves at 125, 250, 375 and 500 μL kg-1 b.wt. on some biochemical parameters of Wistar rats were studied. There was no significant difference in packed cell volume, mean corpuscular hemoglobin concentration, monocytes, lymphocytes, eosinophils, basophils, serum alanine transaminase, liver and kidney body weight ratios. However, the 125 μL kg-1 b.wt. resulted in significant decrease in red blood cell and hemoglobin, whereas the same dose produced increase in mean corpuscular volume, mean corpuscular hemoglobin, neutrophils and large unstained cell. Sodium, inorganic phosphorus, conjugated bilirubin, albumin, globulin and total protein at all the doses were not affected while potassium, chloride, urea, creatinine and total bilirubin concentration were increased at certain doses. Whereas the activity of serum gamma glutamyl transferase was significantly increased at all the doses, those of serum aspartate transaminase were increased only at 125 and 500 μL kg-1 b.wt. The kidney-body weight ratio was increased only at 375 and 500 μL kg-1 b.wt. The result showed that the oil of T. minuta had a mild effect on the parameters investigated at certain doses. This dose and parameter specific effects may influence the use of the essential oil from T. minuta as an insecticide against Sitophilus zeamais in maize grains meant for human and animal consumption.
Evaluation of the potential insecticide activity of Tagetes minuta (Asteraceae) essential oil against the head lice Pediculus humanus capitis (Phthiraptera: Pediculidae)
Cestari, Ivanice M.;Sarti, Silvio J.;Waib, Cláudia M.;Branco Jr., Armando Castello;
Neotropical Entomology , 2004, DOI: 10.1590/S1519-566X2004000600021
Abstract: pediculosis is a worldwide head infestation caused by pediculus humanus capitis de geer. resistance to chemical active ingredients shows the importance of new approaches for head lice control. the aim of the present work was to evaluate the potential insecticide activity of the medicinal plant tagetes minuta (asteraceae) against p. humanus capitis as well as to investigate histopathological aspects to find the target sites of the botanical extract in the insect. lice were collected from heads of infested children 6-12 years old from marília, s?o paulo state, brazil. all the procedures from the collection to the insecticide evaluation were done according to the world health organization protocols. batches of 10 lice each were released onto a clean piece of filter paper in a petri dish. a 100 ppm concentration of essential oil impregnated filter papers were made by dropping 0.1 ml of t. minuta essential oil. petri dishes were placed in a rearing chamber (30oc and 70% relative humidity) in the dark. eight replicates were used for treatment and two replicates for control batches. the median lethal time (lt50) was 16.4 ± 1.62 min. for histophatological investigation, twenty treated and untreated lice were fixed in 10% solution of formalin for 24h and processed according to classical histology techniques. examined cross sections of treated adults showed disassembly of actin and myosin filaments arrangement when compared to untreated adults. this aspect allied to the lt50 obtained suggests a toxic action of the t. minuta essential oil.
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