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Ultrasensitive determination for picomolar-level midecamycin in human serum by flow injection chemiluminescence using luminol–BSA system  [PDF]
Hairu Lv,Xijuan Tan,Yun Zhang,Zhenghua Song
Spectroscopy: An International Journal , 2011, DOI: 10.3233/spe-2012-0563
Abstract: An ultrasensitive method for determining picomolar midecamycin (MID) by flow injection (FI) chemiluminescence (CL) was first described based on the inhibitory effect of MID on luminol–BSA reaction. It was found that the CL intensity decrements were linear with the logarithm of MID concentrations in the range of 1.0–5000 pmol · l–1 with a detection limit as low as 0.3 pmol · l–1 (3σ). The relative standard deviation of seven repetitive measurements for 10 pmol · l–1 MID was 3.0%. At a flow rate of 2.0 ml · min–1, the whole analysis procedure including sampling and washing could be finished in 30 s, offering the sample efficiency of 120 h–1. This proposed method was successfully applied to determine MID in human serum samples with the recoveries from 96.0 to 110.0%. The CL mechanism of luminol–BSA–MID reaction was also given.
Chemiluminescence determination of mezlocillin by the luminol-potassium periodate system
Shi, Wen Bing;Yang, Ji Dong;
Journal of the Brazilian Chemical Society , 2008, DOI: 10.1590/S0103-50532008000600019
Abstract: a new, simple and sensitive method has been proposed for the determination of mezlocillin. it is based on the enhancement of the chemiluminescence (cl) emission generated from the oxidation of luminol in alkaline medium by postassium periodate. the optimization of the experimental and instrumental variables affecting the cl enhancement effect has been carried out using flow-injection system. in the optimum conditions, the method is efficient to determine mezlocillin in the linear range of 0.01-30×10-6 g ml-1 with a detection limit (3σ) of 3.0×10-9 g ml-1 and the relative standard deviation (rsd) is 1.0% for 1.0×10-6 g ml-1 mezlocillin (n=11). it has been successfully applied to the mezlocillin determination in commercial preparations, synthetic samples and biological fluids formulations.
A Sensitive Chemiluminescence Method for Determination of Hydroquinone and Catechol  [PDF]
Lijun Zhao,Baoqiang Lv,Hongyan Yuan,Zaide Zhou,Dan Xiao
Sensors , 2007, DOI: 10.3390/s7040578
Abstract: A novel flow-injection chemiluminescence(CL)method has been developedfor the determination of hydroquinone (HQ) and catechol (CT), based on their inhibition ofthe chemiluminescence reaction of luminol–KMnO4–β-cyclodextrin (β-CD). It was foundthat β-cyclodextrin could effectively enhance the chemiluminescence produced from thereaction of luminol with KMnO4 in basic media. The proposed method is simple, rapid,convenient and sensitive, has a linear range of 1–20 ng/mL for catechol with a detectionlimit of 0.4 ng/mL, and 1–10 ng/mL for hydroquinone with a detection limit of 0.1 ng/mL,respectively. The possible mechanism of the CL reaction is also discussed
Flow injection chemiluminescence determination of levofloxacin in medicine and biological fluids based on its enhancing effect on luminol–H2O2 reaction  [PDF]
Xiaodong Shao,Ying Li,Yangqin Liu,Zhenghua Song
Spectroscopy: An International Journal , 2009, DOI: 10.3233/spe-2009-0379
Abstract: Levofloxacin{(–)-(S)-9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid} is a synthetic fluorinated quinolone derivative, having activity against both Gram (+) and Gram (–) bacteria (aerobic and anaerobic) through inhibition of their DNA gyrase. In this paper, a simple flow injection chemiluminescence with luminol–hydrogen peroxide system was described for determining levofloxacin. The chemiluminescence intensity in the presence of levofloxacin was remarkably enhanced compared with that in the absence of it. Under the optimum reaction conditions the chemiluminescence increment produced was proportional to the concentration of levofloxacin in the range of 1.0–700.0 ng ml–1 (R2═0.9992), with a detection limit of 0.3 ng ml–1 (3σ). At the flow rate of 2.0 ml min–1, the whole process including sampling and washing could be completed in 0.5 min offering the sampling efficiency of 120 times h–1 accordingly, and the relative standard deviation (RSD) was less than 3.0% (n═5). The recovery for the levofloxacin samples was from 95.9% to 104.5%. It was satisfactory for the application to determine levofloxacin in pharmaceutical preparations, human urine and serum samples.
Determination of Nanogram Quantities of Emodin in Pharmaceutical Preparations and Biofluids by Luminol-Myoglobin Chemiluminescence System
Yun Zhang,Xili He,Zhenghua Song
Spectroscopy: An International Journal , 2012, DOI: 10.1155/2012/741053
Abstract: Based on the quenching effect of emodin on the luminol-myoglobin (Mb) reaction, a sensitive method for the determination of nanogram level emodin by flow injection chemiluminescence (FI-CL) is presented for the first time. It was found that the CL intensity from luminol-Mb system could be inhibited in the presence of emodin, and the decrement of CL intensity was linearly proportional to the logarithm of emodin concentration in the range of 0.5–300ng?mL?1 (R = 0.9956) with the detection limit of 0.2ng?mL?1 (3σ). At a flow rate of 2.0mL?min?1, a complete determination of emodin, including sampling and washing, could be accomplished in 0.5 min with the relative standard deviations (RSDs) of less than 3.5% (n = 5). The proposed method was successfully applied to the determination of emodin in pharmaceutical preparations and human serum samples. The possible CL mechanism of luminol-Mb-emodin reaction was explained.
Rapid assay of picogram level of sudan I in hot chilli sauce by flow injection chemiluminescence  [PDF]
Xiaofei Gao,Houyong Liu,Zhenghua Song,Xili He,Faxin Dong
Spectroscopy: An International Journal , 2007, DOI: 10.1155/2007/704349
Abstract: A novel chemiluminescence method for the assay of sudan I was designed using flow injection with chemiluminescence detection. The proposed method was based on the increment effect of sudan I on the chemiluminescence intensity in the luminol–KIO4 system. The increment of chemiluminescence intensity was correlated with the sudan I concentration in the range from 0.1 to 10 pg ml?1, and the determination could be performed in 0.5 min in flow rate of 2 ml min?1, including sampling and washing, giving a throughput of 120 h?1 with a relative standard deviation of less than 5.0%. The method had been successfully applied to the assay of sudan I in Pixian douban, Golden mark guilin chilli sauce and Golden mark satay sauce, and the recovery was 90.0–103.8%.
Chemiluminescence detection of permanganate index (CODMn) by a luminol-KMnO4 based reaction
Chemiluminescence detection of permanganate index (CODMn) by a luminol-KMnO4 based reaction

TIAN Jinjun,HU Yonggang,ZHANG Jie,
TIAN Jinjun
,HU Yonggang,ZHANG Jie

环境科学学报(英文版) , 2008,
Abstract: A novel chemiluminescence (CL) system for determination of permanganate index (CODMn) combined with flow injection analysis has been proposed in this study. On the basis of the chemiluminescent reaction of luminol-KMnO4 system, light emission caused by luminol-KMnO4 system was detected by the photomultiplier tube, and its intensity caused by the appearance of KMnO4 after sample digestion was inversely proportional to CODMn. Effects for CODMn determining such as pH, concentrations and interference were investigated in detail. A detection limit of 0.3 mg/L CODMn with a linear range of 0.3-200 mg/L for its theoretical CODMn was obtained under the optimized experimental conditions. The relative standard deviation was 4.3% for 5.0 mg/L CODMn (n = 11). This CL flow system for determining CODMn was simple, rapid, and suitable for automatic analysis. The data obtained by the present method were fairly in good agreement with those obtained by the standard titrimetric method. It has been applied to determine real samples with satisfactory results.
AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE
CAI,LONGFEI; XU,CHUNXIU;
Journal of the Chilean Chemical Society , 2011, DOI: 10.4067/S0717-97072011000400025
Abstract: a simple and automated method for determination of ascorbic acid (aa) in complex samples by flow injection chemiluminescence (fi-cl) was presented. it is based on the inhibitory effect of ascorbic acid on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system. a home-made micro column of cation exchange resin was adapted to the fi manifold to achieve on-line removal of interference. this method was used to determine the aa content in urine samples with a detection limit of 6.03 x109 mol/l aa and recovery of 93.1-111.0%. this proposed method allows high - throughput determination of aa in urine samples without chromatographic separation, demonstrating that the fi-cl method has significant potential in determination of species in complex samples by adapting a micro column filled with various functionalized materials to the fi manifold.
AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE  [cached]
LONGFEI CAI,CHUNXIU XU
Journal of the Chilean Chemical Society , 2011,
Abstract: A simple and automated method for determination of ascorbic acid (AA) in complex samples by flow injection chemiluminescence (FI-CL) was presented. It is based on the inhibitory effect of ascorbic acid on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system. A home-made micro column of cation exchange resin was adapted to the FI manifold to achieve on-line removal of interference. This method was used to determine the AA content in urine samples with a detection limit of 6.03 x10(9) mol/L AA and recovery of 93.1-111.0%. This proposed method allows high - throughput determination of AA in urine samples without chromatographic separation, demonstrating that the FI-CL method has significant potential in determination of species in complex samples by adapting a micro column filled with various functionalized materials to the FI manifold.
CuFe2O4纳米粒子增敏Luminol-EDTA化学发光体系研究及应用
Enhancement of chemiluminescence of Luminol-EDTA system by CuFe2O4 nanoparticals and its application
 [PDF]

陈华,杨敏,陈祖琴
- , 2017, DOI: 10.11835/j.issn.1000-582X.2017.04.008
Abstract: 基于CuFe2O4纳米粒子能显著增强Luminol-EDTA体系的发光,首次建立了Luminol-EDTA-CuFe2O4 NPs化学发光新体系。紫外吸收光谱和化学发光光谱表明纳米CuFe2O4注入Luminol-EDTA体系后,未生成新发光物质,结合纳米CuFe2O4的特性,提出了CuFe2O4 NPs参与Luminol-EDTA体系可能的发光机理。研究发现芦丁能抑制Luminol-EDTA-CuFe2O4 NPs体系的化学发光,结合流动注射技术,将此化学发光体系应用于芦丁片中芦丁含量的测定。在优化实验条件下,芦丁浓度在2×10-8~2×10-5 mol/L范围内芦丁浓度的对数和相对化学发光值呈线性,芦丁浓度检出限(LOD)为1.21×10-9 mol/L。将本方法应用于芦丁片中的芦丁含量测定,回收率为97%~102%,RSD为2.54%(c=1×10-7mol/L,n=11)。
A new chemiluminescence system of Luminol-EDTA-CuFe2O4 NPs was firstly developed based on the fact that CuFe2O4 nanoparticals (NPs) could significantly enhance the chemiluminescence (CL) intensity of the Luminol-EDTA system. The UV-Vis spectra and the chemiluminescence spectra suggested that no new luminophor formed after the introducing of CuFe2O4 NPs in the Luminol-EDTA system. The possible mechanism of CuFe2O4 NPs participated in Luminol-EDTA system was proposed by combining with the property of CuFe2O4 NPs. Additionally, it was found that rutin could inhibit the chemiluminescence of the proposed Luminol-EDTA-CuFe2O4 NPs system. Combined with flow injection, the system was applied to the determination of rutin in rutin tablets. Under the optimal conditions, the relationship between the relative CL intensity and the logarithm of concentration of rutin was linear when the concentration of ratin ranged from 2×10-8 mol/L to 2×10-5 mol/L with the detection limit of 1.21×10-9 mol/L. The recommended method has been successfully applied to the determination of rutin tablets and the recovery was between 97%-102% and RSD (c=1×10-7mol/L, n=11) was 2.54%
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