Search Results: 1 - 10 of 100 matches for " "
All listed articles are free for downloading (OA Articles)
Page 1 /100
Display every page Item
Molecular Mechanisms Underlying Anti-Inflammatory Actions of 6-(Methylsulfinyl)hexyl Isothiocyanate Derived from Wasabi (Wasabia japonica)  [PDF]
Takuhiro Uto,De-Xing Hou,Osamu Morinaga,Yukihiro Shoyama
Advances in Pharmacological Sciences , 2012, DOI: 10.1155/2012/614046
Abstract: 6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC) is a major bioactive compound in wasabi (Wasabia japonica), which is a typical Japanese pungent spice. Recently, in vivo and in vitro studies demonstrated that 6-MSITC has several biological properties, including anti-inflammatory, antimicrobial, antiplatelet, and anticancer effects. We previously reported that 6-MSITC strongly suppresses cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and cytokines, which are important factors that mediate inflammatory processes. Moreover, molecular analysis demonstrated that 6-MSITC blocks the expressions of these factors by suppressing multiple signal transduction pathways to attenuate the activation of transcriptional factors. Structure-activity relationships of 6-MSITC and its analogues containing an isothiocyanate group revealed that methylsulfinyl group and the length of alkyl chain of 6-MSITC might be related to high inhibitory potency. In this paper, we review the anti-inflammatory properties of 6-MSITC and discuss potential molecular mechanisms focusing on inflammatory responses by macrophages. 1. Introduction Isothiocyanates (ITCs) are a group of naturally occurring sulfur compounds containing –N=C=S functional group, available often abundantly from many cruciferous vegetables. ITCs are stored as glucosinolate precursors in the plants. The damage of plant tissue such as chopping and mastication activates myrosinase which hydrolyses the glucosinolate (myrosinase-glucosinolate system), and the resultant ITCs play a key role in the defense against herbivores and pathogens [1, 2]. There are a significant number of naturally occurring and synthetic ITCs, and numerous studies have demonstrated the chemopreventive and anti-inflammatory properties of ITCs in vitro and in vivo [3–5]. Accumulating evidence suggests that ITCs exert their effects through a variety of signaling pathways involved in detoxification, inflammation, apoptosis, and cell cycle regulation, among others [4–6]. Wasabi (Wasabia japonica) is a member of the Brassicaceae family of vegetables, and its rhizome is a very popular pungent spice in Japan. Several studies have shown that wasabi has multiple physiological functions, such as appetite enhancement [7], antimicrobial activity [8], inhibition of platelet aggregation [9], and the suppression of N-methyl- -nitro-N-nitrosoguanidine-induced rat gastric carcinogenesis [10]. Wasabi differs from other Brassicaceae species in that it contains higher concentration of ITCs, especially long-chain ITCs. The bioactive components of wasabi have
Benzyl Isothiocyanate Suppresses Pancreatic Tumor Angiogenesis and Invasion by Inhibiting HIF-α/VEGF/Rho-GTPases: Pivotal Role of STAT-3  [PDF]
Srinivas Reddy Boreddy, Ravi P. Sahu, Sanjay K. Srivastava
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0025799
Abstract: Our previous studies have shown that benzyl isothiocyanate (BITC) suppresses pancreatic tumor growth by inhibiting STAT-3; however, the exact mechanism of tumor growth suppression was not clear. Here we evaluated the effects and mechanism of BITC on pancreatic tumor angiogenesis. Our results reveal that BITC significantly inhibits neovasularization on rat aorta and Chicken-Chorioallantoic membrane. Furthermore, BITC blocks the migration and invasion of BxPC-3 and PanC-1 pancreatic cancer cells in a dose dependant manner. Moreover, secretion of VEGF and MMP-2 in normoxic and hypoxic BxPC-3 and PanC-1 cells was significantly suppressed by BITC. Both VEGF and MMP-2 play a critical role in angiogenesis and metastasis. Our results reveal that BITC significantly suppresses the phosphorylation of VEGFR-2 (Tyr-1175), and expression of HIF-α. Rho-GTPases, which are regulated by VEGF play a crucial role in pancreatic cancer progression. BITC treatment reduced the expression of RhoC whereas up-regulated the expression of tumor suppressor RhoB. STAT-3 over-expression or IL-6 treatment significantly induced HIF-1α and VEGF expression; however, BITC substantially suppressed STAT-3 as well as STAT-3-induced HIF-1α and VEGF expression. Finally, in vivo tumor growth and matrigel-plug assay show reduced tumor growth and substantial reduction of hemoglobin content in the matrigel plugs and tumors of mice treated orally with 12 μmol BITC, indicating reduced tumor angiogenesis. Immunoblotting of BITC treated tumors show reduced expression of STAT-3 phosphorylation (Tyr-705), HIF-α, VEGFR-2, VEGF, MMP-2, CD31 and RhoC. Taken together, our results suggest that BITC suppresses pancreatic tumor growth by inhibiting tumor angiogenesis through STAT-3-dependant pathway.
Shoot Regeneration from Wasabi (Wasbia japonica Mtsum) Callus  [PDF]
M. Hassan,Y. Fujime,T. Matsui,N. Okuda
Journal of Biological Sciences , 2001,
Abstract: Adventitious shoot formation was induced on callus of wasabi. Adventitious buds were formed from subculture of callus on modified MS media (containing 0.002 mg/ l TDZ (n-phenyl-N- 1, 2, 3- thidiazol-5-yl-urea or thidiazuron) and 1 mg/ l of NAA (naphhaleneacetic acid) were failed to elongate. Bud forming calli were subcultured in modified MS media containing BA, (6-benzyladenine) or kinetin with or without NAA or NAA alone. Shoot development was observed on the enlarged callus in media containing BA or kinetin with NAA. While media containing BA or kinetin without NAA and media containing only NAA did not result in organ formation. Calli subcultured on hormone free media caused the buds to become black and die out. Shoots formed in the presence of kinetin were either smaller in size or turned brown in colour. Green and plump shoots were formed in media containing 0.5 or 1 mg/ l of BA with 0.5 mg/ l of NAA. Higher doses of BA and NAA caused the shoots to become thin and branched. Calli that produced the best shoots also grew faster than others having a lush green appearance. However, root formation was less frequent in shoot forming calli. Root formation was most frequent when kinetin was used as a source of cytokinin.
Diffusion and sorption of allyl isothiocyanate in the process of fumigation of maize
Paes, Juliana L.;Faroni, Lêda R. A.;Martins, Marcio A.;Dhingra, Onkar D.;Silva, Tales A.;
Revista Brasileira de Engenharia Agrícola e Ambiental , 2011, DOI: 10.1590/S1415-43662011000300011
Abstract: the purpose of this work was to evaluate the transport of allyl isothiocyanate (aitc) by means of the sorption mechanism and the diffusion of aitc through maize grains. the experimental procedure to study the sorption mechanism was conducted by injection of aitc in glass flasks containing the grains. kinetic models of zero, first and second order were fitted to aitc concentration data, with the purpose of determining the sorption rate (k). for the study of diffusion, a prototype was utilized in which the components are a gas chamber and a cylindrical column in which the grains were stored. at the interval of 110 min, samples in the chamber and in the column were collected, which were immediately injected into the gas chromatograph. in order to determine the effective diffusion coefficient (def) of aitc through maize grains, the term referring to the sorption rate was added to the diffusion equation. the kinetic model of the first order was the one that best fitted the experimental data for the sorption mechanism, and k was 6.26 x 10-4 s-1. the def of aitc through grains was 7.2 x 10-3 cm2 s-1. it was concluded that the transport of aitc through maize grains is slow, due to the low value of def and high sorption rate of this component by the grains.
Allyl isothiocyanate induced stress response in Caenorhabditis elegans
AkalRachna K Saini, Robert T Tyler, Youn Shim, Martin JT Reaney
BMC Research Notes , 2011, DOI: 10.1186/1756-0500-4-502
Abstract: An assay to determine the concentration of AITC in ground mustard seed was improved to allow the measurement of AITC release in the first minutes after exposure of ground mustard seed to water. Using this assay, we determined that temperatures above 67°C decreased sinigrin conversion to AITC in hydrated ground B. juncea seed. A pH near 6.0 was found to be necessary for AITC release. RT-qPCR revealed no significant change in HSP70A mRNA expression at low concentrations of AITC (< 0.1 μM). However, treatment with higher concentrations (> 1.0 μM) resulted in a four- to five-fold increase in expression. A HSP70 ELISA showed that AITC toxicity in C. elegans was ameliorated by the presence of ground seed from low sinigrin B. juncea cv. Arrid.? AITC induced toxicity in C. elegans, as measured by HSP70 expression.? Conditions required for the conversion of sinigrin to AITC in ground B. juncea seed were determined.? The use of C. elegans as a bioassay to test AITC or mustard biopesticide efficacy is discussed.Plant seeds have evolved a broad spectrum of natural defense mechanisms, such as physical and chemical barriers. Mustard species mitigate a wide range of biotic challenges using the glucosinolate-myrosinase system, also referred to as 'The Mustard Bomb' [1]. Glucosinolates (glucoraphanin, glucoerucin, gluconasturtiin, sinigrin, glucotropaeolin, glucoraphenin, glucoraphasatin, glucomoringin and glucobrassicin) are hydrolysed by the enzyme myrosinase (thioglucosidase) to produce an aglycone, which undergoes spontaneous non-enzymatic rearrangement to produce organic isothiocyanates, thiocyanates, nitriles, epithionitriles, oxazolidinethiones and organic cyanates [2-4].Many glucosinolate products, including allyl isothiocyanate (AITC), are of interest because of their broad spectra of biological activities. For example, the toxicity of Indian mustard and AITC were demonstrated on masked chafer Beetle larvae [5]. The biopesticidal [6,7], fungicidal [6,8], antibiotic [9,10] a
Removal of Di-2-Ethyl Hexyl Phthalates by Membrane Bioreactor  [PDF]
Salah Al Sakiti, Suwanna Kitpati Boontanon, Narin Boontanon
Journal of Environmental Protection (JEP) , 2013, DOI: 10.4236/jep.2013.44045

A lab scale membrane bioreactor system was built to investigate the removal of Di-2-Ethyl Hexyl Phthalates (DEHP) in wastewater under variation of three runs: two hydraulic retention time (HRT) 24 and 36 hours in addition to two biomass: concentrated and light sludge. Solid phase extraction (SPE) followed by high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) was applied to quantitatively identify DEHP in wastewater samples. Membrane bioreactor was built and operated to investigate DEHP removal. Higher HRT removed DEHP more efficiently than lower HRT. The concentrated MLSS could achieve higher removal efficiency than the lighter MLSS at the same HRT value. The performance of MBR in removing DEHP, TOC and COD from wastewater with a maximum removal efficiency were 29%, 85%, and 98%, respectively. Mass balance of DEHP in the system indicated that a majority was removed by adsorption process rather than filtration or microbiological process.

BITC Sensitizes Pancreatic Adenocarcinomas to TRAIL-induced Apoptosis
Christina A. Wicker, Ravi P. Sahu, Kashmira Kulkarni-Datar, Sanjay K. Srivastava and Thomas L. Brown
Cancer Growth and Metastasis , 2012,
Abstract: Pancreatic adenocarcinoma is an aggressive cancer with a greater than 95% mortality rate and short survival after diagnosis. Chemotherapeutic resistance hinders successful treatment. This resistance is often associated with mutations in codon 12 of the K-Ras gene (K-Ras 12), which is present in over 90% of all pancreatic adenocarcinomas. Codon 12 mutations maintain Ras in a constitutively active state leading to continuous cellular proliferation. Our study determined if TRAIL resistance in pancreatic adenocarcinomas with K-Ras 12 mutations could be overcome by first sensitizing the cells with Benzyl isothiocyanate (BITC). BITC is a component of cruciferous vegetables and a cell cycle inhibitor. BxPC3, MiaPaCa2 and Panc-1 human pancreatic adenocarcinoma cell lines were examined for TRAIL resistance. Our studies show BITC induced TRAIL sensitization by dual activation of both the extrinsic and intrinsic apoptotic pathways.
Dietary agent, benzyl isothiocyanate inhibits signal transducer and activator of transcription 3 phosphorylation and collaborates with sulforaphane in the growth suppression of PANC-1 cancer cells
Hutzen Brian,Willis William,Jones Sarah,Cen Ling
Cancer Cell International , 2009, DOI: 10.1186/1475-2867-9-24
Abstract: The Signal Transducer and Activator of Transcription (STAT) proteins comprise a family of latent transcription factors with diverse functions. STAT3 has well established roles in cell proliferation, growth and survival, and its persistent activation has been detected with high frequency in many human cancers. As constitutive activation of STAT3 appears to be vital for the continued survival of these cancerous cells, it has emerged as an attractive target for chemotherapeutics. We examined whether the inhibitory activities of bioactive compounds from cruciferous vegetables, such as Benzyl isothiocyanate (BITC) and sulforaphane, extended to STAT3 activation in PANC-1 human pancreatic cancer cells. BITC and sulforaphane were both capable of inhibiting cell viability and inducing apoptosis in PANC-1. Sulforaphane had minimal effect on the direct inhibition of STAT3 tyrosine phosphorylation, however, suggesting its inhibitory activities are most likely STAT3-independent. Conversely, BITC was shown to inhibit the tyrosine phosphorylation of STAT3, but not the phosphorylation of ERK1/2, MAPK and p70S6 kinase. These results suggest that STAT3 may be one of the targets of BITC-mediated inhibition of cell viability in PANC-1 cancer cells. In addition, we show that BITC can prevent the induction of STAT3 activation by Interleukin-6 in MDA-MB-453 breast cancer cells. Furthermore, combinations of BITC and sulforaphane inhibited cell viability and STAT3 phosphorylation more dramatically than either agent alone. These findings suggest that the combination of the dietary agents BITC and sulforaphane has potent inhibitory activity in pancreatic cancer cells and that they may have translational potential as chemopreventative or therapeutic agents.
2-(4-Chlorophenyl)-5-fluoro-3-methylsulfinyl-1-benzofuran  [cached]
Hong Dae Choi,Pil Ja Seo,Byeng Wha Son,Uk Lee
Acta Crystallographica Section E , 2010, DOI: 10.1107/s1600536810001157
Abstract: In the title compound, C15H10ClFO2S, the O atom and the methyl group of the methylsulfinyl substituent are located on opposite sides of the plane through the benzofuran fragment. The 4-chlorophenyl ring is rotated out of the benzofuran plane, as indicated by the dihedral angle of 21.04 (4)°. The crystal structure exhibits a weak intermolecular C—H...O hydrogen bond and a Cl...O halogen bond [Cl...O = 3.254 (1) ].
2-(4-Fluorophenyl)-5-iodo-3-methylsulfinyl-1-benzofuran  [cached]
Hong Dae Choi,Pil Ja Seo,Byeng Wha Son,Uk Lee
Acta Crystallographica Section E , 2010, DOI: 10.1107/s1600536809051642
Abstract: In the title compound, C15H10FIO2S, the O atom and the methyl group of the methylsulfinyl substituent are located on opposite sides of the plane through the benzofuran fragment. The 4-fluorophenyl ring is rotated out of the benzofuran plane by a dihedral angle of 28.33 (5)°. The crystal structure is stabilized by a weak non-classical intermolecular C—H...O hydrogen bond and an I...O halogen interaction [3.211 (1) ].
Page 1 /100
Display every page Item

Copyright © 2008-2017 Open Access Library. All rights reserved.