Objective: The aim of the study was to
improve the therapeutic effect for lung cancer using a synergetic strategy of
adenovirus-based gene therapy combined with chemotherapy. Methods: A conditional replication adenovirus(CRAd) was employed
to treat the A549 lung cancer cells and cisplatin-resisted A549(A549-DDP) cells.
In vitro MTS / PMS assay were used to evaluate
the cell viability. PCR were used to detect expression of Coxsackie receptor (CAR)
and Multidrug resistance(MDR) gene. The in
vivo anti-tumor effect of CRAd and cisplatin was evaluated using a
subcutaneous mouse model. Results: The CRAd sensitizes A549 cancer cells to
cisplatin. The mechanism of enhanced cell growth inhibition is associated with the
increased CAR and MDR expression. Conclusion: Our approach is better than the
conventional gene therapy and chemotherapy strategy.