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Danos de Rhopalosiphum padi (L.) (Hemiptera: Aphididae) no trigo em fun??o da dura??o e da densidade de infesta??o
Roza-Gomes, Margarida F.;Salvadori, José R.;Schons, Jurema;
Neotropical Entomology , 2008, DOI: 10.1590/S1519-566X2008000500013
Abstract: aphids are considered relevant pests on wheat either by direct damage through sap sucking or by indirect damage vectoring bydv (barley yellow dwarf virus). rhopalosiphum padi l. has been observed infesting wheat fields with an increasing frequency. the knowledge and the available technology, besides being more related to other aphids species already recognized as pests, they are insufficient to control the specific problem of r. padi. thus, this work evaluated the effects of feeding duration and infestation densities of r. padi on seedlings of wheat cv. embrapa 16. grain yield, yield components and the extent of symptoms were recorded. the experiment was carried out in the field under a completely randomized split-plot experimental design with four replications. the main plot was feeding duration (two and seven days) and the sub-plots were infestation densities (zero, two and 10 aphids per plant). independent on feeding duration, 10 aphids per plant resulted in significant yield losses, reduction of number of heads and tillers per plant. canopy dry matter was also reduced. infestations of two and 10 aphids per plant resulted in continuous yellowing of wheat plants from tillering to the end of flowering stage. when aphids fed for seven days on wheat, more yellowing symptoms were observed at the flower stage in comparison with two days feeding.
Rhopalosiphum padi Feeding – Attempted Symptomatic Defence Mechanisms in Barley Leaves Include Wound Callose Deposition?  [cached]
Sefiu Adekilekun SAHEED,Christiaan Edward Johannes BOTHA
Notulae Scientia Biologicae , 2010,
Abstract: The deposition of callose and the damage-related symptoms subsequently expressed by infested plants were investigated after feeding on barley leaves by bird cherry-oat aphid (BCA), Rhopalosiphum padi L. Feeding by this aphid does not result in appearance of visible damage to the plants, provided the feeding population is small. Using aniline blue fluorochrome, we confirmed that whilst low feeding density (5 aphids) results in appearance of wound callose in sieve tubes, this only occurs after 14d of feeding, when the feeding population had increased. Continued feeding results in progressively more callose deposition and by 21d, severe damage has been caused. In contrast, feeding by larger populations (50 adult aphids), results in the appearance of wound callose within 72h, in longitudinal and cross veins. We suggest that this wounding response appears to play a role in the appearance of golden yellow streak symptoms reported to occur in leaves where BCA feeding density was high.
Resposta de cultivares de trigo à infesta??o do pulg?o Rhopalosiphum padi
Peruzzo, Rafael;Salvadori, José Roberto;Pereira, Paulo Roberto Valle da Silva;Bertollo, étel Carmen;Tonello, Lucas Simionatto;
Pesquisa Agropecuária Brasileira , 2007, DOI: 10.1590/S0100-204X2007001200003
Abstract: the objective of this work was to evaluate the response of eight commercial wheat cultivars (brs 194, brs 208, brs camboatá, brs guabiju, brs guamirim, brs louro, brs timbaúva e brs umbu) to the infestation of rhopalosiphum padi (hemiptera: aphididae). the experiment was carried out at embrapa trigo, in greenhouse conditions. it was used the randomized block design, in a split-plot array, with eight treatments and six replicates. the plots received treatments with or without infestation with aphids, and the split-plots were composed by the cultivars. the infestation with aphids (20 wingless adults per plant) was done 12 days after plant emergency, in the beginning of tillering, and was kept for 15 days. the cultivars responded differently to the infestation with aphids. brs timbaúva cultivar is more resistant, and brs umbu and brs guabiju cultivars are more susceptible to r. padi, concerning plant growth and plant yield.
Population Growth of Rhopalosiphum padi L. (Homoptera: Aphididae) on Different Cereal Crops from the Semiarid Pampas of Argentina under Laboratory Conditions Crecimiento Poblacional de Rhopalosiphum padi L. (Homoptera: Aphididae) sobre Diferentes Cereales de la Pampas Semiárida de Argentina en Condiciones de Laboratorio  [cached]
Lilian R Descamps,Carolina Sánchez Chopa
Chilean Journal of Agricultural Research , 2011,
Abstract: The bird cherry-oat aphid Rhopalosiphum padi L. (Homoptera: Aphididae) is one of the main pests in a number of crops in the semiarid Pampas of Argentina. In the present study, the effect of different host plants, including Triticum aestivum L., ×Triticosecale Wittm., Hordeum vulgare L., Hordeum distichum L., Avena sativa L., and Secale cereale L. on biological parameters of R. padi L. was studied in the laboratory at 24 ± 1 °C, 65 ± 10% RH and a 14:10 photoperiod. Longevity, intrinsic rate of natural increase (r m), net reproductive rate (R0), mean generation time (T), doubling time (DT), and finite rate of increase (λ) of the bird cherry-oat aphid on the different cereal crops were estimated. Differences in fertility life table parameters of R. padi among host plants were analyzed using pseudo-values, which were produced by Jackknife re-sampling. Results indicated that beer barley might be the most suitable food for R. padi due to greater adult longevity (20.88 d), higher fecundity (41 nymphs female-1), higher intrinsic rate of natural increase (0.309 females female-1 d-1), lower doubling time (2.24), and lower nymphal mortality (22.2%). Therefore, it can be concluded from the present study that R. padi prefers beer barley for fast and healthy development over other cereal crops. El áfido Rhopalosiphum padi L. (Homoptera: Aphididae) es una de las principales plagas de numerosos cultivos de la región semiárida pampeana de Argentina. En el presente trabajo se estudió el efecto de diferentes cereales incluidos Triticum aestivum L., ×Triticosecale Wittm., Hordeum vulgare L., Hordeum distichum L., Avena sativa L. and Secale cereale L. sobre los parámetros biológicos de R. padi en laboratorio. Se estimaron longevidad, tasa intrínseca de crecimiento natural (r m), tasa neta de reproducción (R0), tiempo generacional medio (T), tiempo de duplicación (TD), y tasa finita de incremento (λ) del pulgón de la avena en diferentes cereales. Las diferencias de los parámetros biológicos de R. padi entre los distintos cultivos fueron analizadas utilizando pseudovalores con la técnica de Jackknife. Los resultados indican que la cebada cervecera podría ser el cultivo más preferido por este áfido debido a la larga longevidad (20,88 d), la alta fecundidad (41 ninfas hembra-1), la alta tasa de incremento natural (0,309 hembras hembra-1 d-1), el corto tiempo de duplicación (2,24 d) y la baja mortalidad ninfal (22,2%). De los resultados obtenidos podemos inferir que R. padi tiene una mejor performance sobre la cebada cervecera que sobre el resto de cereales utilizados en este tr
Population Growth of Rhopalosiphum padi L. (Homoptera: Aphididae) on Different Cereal Crops from the Semiarid Pampas of Argentina under Laboratory Conditions
Descamps,Lilian R; Sánchez Chopa,Carolina;
Chilean journal of agricultural research , 2011, DOI: 10.4067/S0718-58392011000300007
Abstract: the bird cherry-oat aphid rhopalosiphum padi l. (homoptera: aphididae) is one of the main pests in a number of crops in the semiarid pampas of argentina. in the present study, the effect of different host plants, including triticum aestivum l., ×triticosecale wittm., hordeum vulgare l., hordeum distichum l., avena sativa l., and secale cereale l. on biological parameters of r. padi l. was studied in the laboratory at 24 ± 1 °c, 65 ± 10% rh and a 14:10 photoperiod. longevity, intrinsic rate of natural increase (rm), net reproductive rate (r0), mean generation time (t), doubling time (dt), and finite rate of increase (λ) of the bird cherry-oat aphid on the different cereal crops were estimated. differences in fertility life table parameters of r. padi among host plants were analyzed using pseudo-values, which were produced by jackknife re-sampling. results indicated that beer barley might be the most suitable food for r. padi due to greater adult longevity (20.88 d), higher fecundity (41 nymphs female-1), higher intrinsic rate of natural increase (0.309 females female-1 d-1), lower doubling time (2.24), and lower nymphal mortality (22.2%). therefore, it can be concluded from the present study that r. padi prefers beer barley for fast and healthy development over other cereal crops.
Cloning and Prokaryotic Expression of Viral Binding Protein(VBP) Gene From Endosybiotic Bacterium of Rhopalosiphum padi
禾谷缢管蚜体内的病毒结合蛋白基因的克隆与原核表达

Wu Yunfeng,Cui Xiaofeng,Lin Lin,Zhou Guanghe,
吴云锋
,崔晓锋,林林,周广和

微生物学报 , 2002,
Abstract: Viral binding protein gene from Rhopalosiphum padi Yangling biotype was amplified by PCR method and then cloned.The complete nucleotide sequence of the gene was determined,It has 1647 nucleotides encoding 548 amino acids.Comparison showed this gene has 97% identity on nucleotide level with Buchnera groEL AM gene of Rhopalosiphum padi American biotype,while has 97.4% identity on amino acid level was found between this two genes.The VBP gene was ligated into pBV221 and pET30a expression vector and expressed the aim protein 63kD and 69kD.
Double Subgenomic Alphaviruses Expressing Multiple Fluorescent Proteins Using a Rhopalosiphum padi Virus Internal Ribosome Entry Site Element  [PDF]
Michael R. Wiley,Lisa O. Roberts,Zach N. Adelman,Kevin M. Myles
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0013924
Abstract: Double subgenomic Sindbis virus (dsSINV) vectors are widely used for the expression of proteins, peptides, and RNA sequences. These recombinant RNA viruses permit high level expression of a heterologous sequence in a wide range of animals, tissues, and cells. However, the alphavirus genome structure and replication strategy is not readily amenable to the expression of more than one heterologous sequence. The Rhopalosiphum padi virus (RhPV) genome contains two internal ribosome entry site (IRES) elements that mediate cap-independent translation of the virus nonstructural and structural proteins. Most IRES elements that have been characterized function only in mammalian cells but previous work has shown that the IRES element present in the 5′ untranslated region (UTR) of the RhPV genome functions efficiently in mammalian, insect, and plant systems. To determine if the 5′ RhPV IRES element could be used to express more than one heterologous sequence from a dsSINV vector, RhPV 5′ IRES sequences were placed between genes for two different fluorescent marker proteins in the dsSINV, TE/3′2J/mcs. While mammalian and insect cells infected with recombinant viruses containing the RhPV sequences expressed both fluorescent marker proteins, only single marker proteins were routinely observed in cells infected with dsSINV vectors in which the RhPV IRES had been replaced by a luciferase fragment, an antisense RhPV IRES, or no intergenic sequence. Thus, we report development of a versatile tool for the expression of multiple sequences in diverse cell types.
模拟气候变暖对不同纬度带麦长管蚜和禾谷缢管蚜种群动态的影响
Impact of simulated warming on the population dynamics of grain aphid Sitobion avenae and bird cherry-oat aphid Rhopalosiphum padi across latitudes
 [PDF]

韩宗礼,谭晓玲,范佳,孙京瑞,陈巨莲,Han Zongli,Tan Xiaoling,Fan Jia,Sun Jingrui,Chen Julian
- , 2018, DOI: 10.13802/j.cnki.zwbhxb.2018.2017073
Abstract: 为明确气候变暖对不同纬度带麦田麦长管蚜Sitobion avenae和禾谷缢管蚜Rhopalosiphum padi种群动态的影响,在高纬度的河北省廊坊市(39°30'N,116°36'E)和相对低纬度的河南省原阳县(34°55'N,114°15'E)开展红外线辐射增温试验,调查廊坊市(+2.00℃)和原阳县(+1.18℃)2个试验点的增温处理小区和对照小区麦长管蚜和禾谷缢管蚜的种群动态,并计算其盛期发生比值。结果表明,增温使廊坊市试验点的麦长管蚜始见期、峰期分别提前21、7 d,使原阳县试验点的麦长管蚜始见期、峰期分别提前14、14 d;而禾谷缢管蚜的始见期和峰期仅在原阳县试验点提前31 d和7 d。增温使廊坊市试验点的麦长管蚜盛期蚜量、峰期蚜量分别显著增加了139.21%和77.83%,禾谷缢管蚜盛期蚜量和峰期蚜量分别显著增加了157.31%和130.16%;增温使原阳县试验点的麦长管蚜盛期蚜量、峰期蚜量分别显著增加了149.62%和176.52%,禾谷缢管蚜/麦长管蚜的盛期蚜量比值显著降低了43.21%。表明增温有利于高纬度地区(廊坊市)麦长管蚜和禾谷缢管蚜种群增长,而在低纬度地区(原阳县)仅有利于麦长管蚜种群增长。
In order to understand the impact of global warming on the population dynamics of the wheat aphids, Sitobion avenae and Rhopalosiphum padi, in wheat fields across different latitudes, the field experiments of temperature-elevation (Te) with infrared radiator heating device were conducted at two latitudes:Langfang City (39°30'N, 116°36'E), Hebei Province and Yuanyang County (34°55'N, 114°15'E), Henan Province. The population dynamics of S. avenae and R. padi were investigated in the two tested areas with Te treatment (+2.00℃) and control in Langfang, and Te treatment (+1.18℃) and control in Yuanyang, and the ratio value of two aphid populations was calculated during the peak stage. The results showed that, for S. avenae, warming advanced the initial appearance and peak period by 21 d, 7 d in Langfang, and 14 d, 14 d in Yuanyang, respectively, but for R. padi, the advancement (by 31 d, 7 d) only occurred in Yuanyang. Warming significantly increased the abundance of S. avenae at peak stage and the peak values were increased by 139.21%, 77.83% in Langfang and 149.62%, 176.52% in Yuanyang, respectively; R. padi increased by 157.31%, 130.16% only in Langfang, but the ratio value of R. padi to S. avenae was significantly decreased by 43.21% in Yuanyang. The results indicated that warming benefited both aphid populations at the relative higher latitude like Langfang, but only benefited S. avenae population at the relative lower latitude like Yuanyang.
禾谷缢管蚜热激蛋白Hsp90基因的克隆和表达分析
Molecular cloning and expression analysis of the Hsp90 gene in the bird cherry-oat aphid, Rhopalosiphum padi
 [PDF]

李玉婷,赵奇,房冰,陈茂华,Li Yuting,Zhao Qi,Fang Bing,Chen Maohua
- , 2017, DOI: 10.13802/j.cnki.zwbhxb.2017.2015183
Abstract: 为探讨高温驯化对禾谷缢管蚜Rhopalosiphum padi Hsp90基因表达量的影响,以及其在不同发育阶段的表达,通过RT-PCR与RACE技术克隆了热激蛋白Hsp90基因的全长,命名为RpHsp90,采用生物信息方法分析了其序列特征,利用实时荧光定量PCR技术研究了RpHsp90在禾谷缢管蚜不同发育阶段和驯化后热激处理的表达量变化。结果显示:禾谷缢管蚜RpHsp90的cDNA全长为2 644 bp,编码727个氨基酸,分子量为83.2 kD。推导的氨基酸序列具有Hsp90蛋白家族的5个签名序列及C末端细胞质特征序列EEVD。系统进化树结果显示,RpHsp90与其它昆虫Hsp90具有很高的相似性。实时荧光定量PCR结果表明,不同发育阶段RpHsp90均有表达,并且4龄若蚜期表达量最高,显著高于其它龄期;27℃热驯化后禾谷缢管蚜RpHsp90表达量和常温24℃处理试虫显著差异;热激处理显著诱导RpHsp90的表达,39℃热激处理RpHsp90表达量显著高于40℃热激处理;热驯化种群经热激处理后表达量显著高于常温热激处理。表明RpHsp90在禾谷缢管蚜不同龄期差异表达,且在该虫对热胁迫的响应中具有重要作用。
To investigate the influence of heat acclimation on heat shock protein 90 in Rhopalosiphum padi and analyze the expression pattern of the gene at different developmental stages, the reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends (RACE) methods were used to amplify the full length cDNA of R. padi Hsp90 gene (designated as RpHsp90). Bioinformatic methods were used to analyze the characteristics of RpHsp90. The expression levels of the RpHsp90 gene at different developmental stages and under different heat treatments were detected by the real-time quantitative PCR. The results showed that the full-length cDNA of RpHsp90 was 2 644 bp, which encoded 727 amino acids, with a molecular weight of 83.2 kD. The deduced amino acid sequence of RpHsp90 contained five signature sequences of Hsp90 family and a cytoplasmic character sequence (EEVD) at C-terminal. The phylogenic tree indicated that RpHsp90 protein shared a high homology with Hsp90 proteins from other insect species. The real-time quantitative PCR analyses showed that the expression levels of RpHsp90 varied remarkably at different developmental stages. The mRNA level was the highest in the 4th-instar nymphs, which was significantly higher than that in other instars. No significant difference in expression level was found between 27℃ acclimation and the control at 24℃. The expression level of RpHsp90 was highly induced by heat shock treatments. The mRNA level of RpHsp90 under 39℃ treatment was significantly higher than that under 40℃ treatment. The population with 27℃ acclimation showed the highest expression level of RpHsp90 after heat shock. The current study indicated that the expression level of RpHsp90 varied at different developmental stages and played a role in the response of the pest to heat stress.
Comparative of feeding behaviors of Sitobion avenae, Sitobion graminum and Rhopalosiphum padi (Homoptera: Aphididae) using electrical penetration graph (EPG)
基于EPG的麦长管蚜、麦二叉蚜和禾谷缢管蚜取食行为比较

MIAO Jin,WU Yuqing,YU Zhenxing,DUAN Yun,JIANG Yueli,LI Guoping,
苗进
,武予清,郁振兴,段云,蒋月丽,李国平

生态学报 , 2011,
Abstract: 麦长管蚜Sitobion avenae (Fabricius),麦二叉蚜Schizaphis graminum (Rondani)和禾谷缢管蚜Rhopalosiphum padi (Linnaeus)虽然都取食小麦,但其取食部位、传毒能力均有差异。利用EPG研究了3种麦蚜在同一寄主植物上的取食行为,结果显示:(1)禾谷缢管蚜在取食过程中G波出现的时间最早,G波总的持续时间和每次刺探G波的平均持续时间都明显长于麦长管蚜和麦二叉蚜。麦长管蚜第1次E1波的时间、E1波总的持续时间和每次刺探E1波的平均持续时间都明显比麦二叉蚜和禾谷缢管蚜要长。麦二叉蚜E2波总的持续时间和每次刺探E2波的平均持续时间都明显长于麦长管蚜和禾谷缢管蚜。表明3种蚜虫各自拥有不同取食策略。禾谷缢管蚜比其它两种蚜虫需要更多的水分和无机盐,麦长管蚜通过分泌大量唾液增大对食物的利用率,麦二叉蚜通过增加韧皮部取食时间以满足对营养的需要。(2)刺探过程中麦二叉蚜pd波出现的次数为(51.97±7.82)次,高于麦长管蚜的(44.73±4.52)次和禾谷缢管蚜的(32.99±4.22)次。麦二叉蚜pd波Ⅱ-2和Ⅱ-3亚波的时间最长,禾谷缢管蚜最短,三者之间差异达显著水平。表明与其它两种麦蚜相比,麦二叉蚜在取食过程中口针与细胞间的接触频率更高,与细胞内获毒和传毒相关的Ⅱ-3波和Ⅱ-2波持续时间更长,增加了获毒和传毒的几率,是其传毒能力最强的机理。
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