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Expression of TERT in precancerous gastric lesions compared to gastric cancer
Duarte, M.C.;Babeto, E.;Leite, K.R.M.;Miyazaki, K.;Borim, A.A.;Rahal, P.;Silva, A.E.;
Brazilian Journal of Medical and Biological Research , 2011, DOI: 10.1590/S0100-879X2010007500143
Abstract: the objective of this study was to determine the levels of tert mrna and tert protein expression in stomach precancerous lesions such as intestinal metaplasia (im) and gastric ulcer (gu) and compare them to gastric cancer (gc). real-time pcr was performed to detect tert mrna expression levels in 35 biopsies of im, 30 of gu, and 22 of gc and their respective normal mucosas. tert protein was detected by immunohistochemistry in 68 samples, 34 of im, 23 of gu, and 11 of gc. increased tert mrna expression levels were observed in a significant number of cases, i.e., 46% of im, 50% of gu, and 79% of gc. the relative mean level of tert mrna after normalization with the β-actin reference gene and comparison with the respective adjacent normal mucosa was slightly increased in the im and gu groups, 2.008 ± 2.605 and 2.730 ± 4.120, respectively, but high tert mrna expression was observed in the gc group (17.271 ± 33.852). however, there were no statistically significant differences between the three groups. tert protein-positive immunostaining was observed in 38% of im, 39% of gu, and 55% of gc. no association of tert mrna and protein expression with helicobacter pylori infection or other clinicopathological variables was demonstrable, except for the incomplete type vs the complete type of im. this study confirms previous data of the high expression of both tert mrna and protein in gastric cancer and also demonstrates this type of changed expression in im and gu, thus suggesting that tert expression may be deregulated in precursor lesions that participate in the early stages of gastric carcinogenesis.
Anti-Helicobacter pylori therapy followed by celecoxib on progression of gastric precancerous lesions  [cached]
Li-Jing Zhang, Shi-Yan Wang, Xiao-Hui Huo, Zhen-Long Zhu, Jian-Kun Chu, Jin-Cheng Ma, Dong-Sheng Cui, Ping Gu, Zeng-Ren Zhao, Ming-Wei Wang, Jun Yu
World Journal of Gastroenterology , 2009,
Abstract: AIM: To evaluate whether celecoxib, a selective cyclooxygenase 2 (COX-2) inhibitor, could reduce the severity of gastric precancerous lesions following Helicobacter pylori (H pylori) eradication.METHODS: H pylori-eradicated patients with gastric precancerous lesions randomly received either celecoxib (n = 30) or placebo (n = 30) for up to 3 mo. COX-2 expression and activity was determined by immunostaining and prostaglandin E2 (PGE2) assay, cell proliferation by Ki-67 immunostaining, apoptosis by TUNEL staining and angiogenesis by microvascular density (MVD) assay using CD31 staining.RESULTS: COX-2 protein expression was significantly increased in gastric precancerous lesions (atrophy, intestinal metaplasia and dysplasia, respectively) compared with chronic gastritis, and was concomitant with an increase in cell proliferation and angiogenesis. A significant improvement in precancerous lesions was observed in patients who received celecoxib compared with those who received placebo (P < 0.001). Of these three changes, 84.6% of sites with dysplasia regressed in patients treated with celecoxib (P = 0.002) compared with 60% in the placebo group, suggesting that celecoxib was effective on the regression of dysplasia. COX-2 protein expression (P < 0.001) and COX-2 activity (P < 0.001) in the gastric tissues were consistently lower in celecoxib-treated patients compared with the placebo-treated subjects. Moreover, it was also shown that celecoxib suppressed cell proliferation (P < 0.01), induced cell apoptosis (P < 0.01) and inhibited angiogenesis with decreased MVD (P < 0.001). However, all of these effects were not seen in placebo-treated subjects. Furthermore, COX-2 inhibition resulted in the up-regulation of PPARγ expression, a protective molecule with anti-neoplastic effects.CONCLUSION: H pylori eradication therapy followed by celecoxib treatment improves gastric precancerous lesions by inhibiting COX-2 activity, inducing apoptosis, and suppressing cell proliferation and angiogenesis.
Precancerous Lesions of the Oral Mucosa  [cached]
Oya Gürbüz
Turkderm , 2012,
Abstract: In this review of oral precancerous lesions, leukoplakia, erythroleukoplakia/erythroplakia and the least common variant proliferative verrucous leukoplakia will be focused with their clinical characteristics and their potential to develop oral squamous cell carcinoma and related factors will be discussed.
Significance and relationship between Yes-associated protein and survivin expression in gastric carcinoma and precancerous lesions  [cached]
Chun-Li Da, Yan Xin, Jing Zhao, Xiang-Dong Luo
World Journal of Gastroenterology , 2009,
Abstract: AIM: To analyze the differences and relevance of Yes-associated protein (YAP) and survivin, and to explore the correlation and significance of their expression in gastric carcinoma and precancerous lesions.METHODS: The PV9000 immunohistochemical method was used to detect the expression of YAP and survivin in 98 cases of normal gastric mucosa, 58 intestinal metaplasia (IM), 32 dysplasia and 98 gastric carcinoma.RESULTS: The positive rates of YAP in dysplasia (37.5%) and gastric carcinoma (48.0%) were significantly higher than that in normal gastric mucosa (13.3%), P < 0.01. The positive rates of survivin in IM (53.4%), dysplasia (59.4%) and gastric carcinoma (65.3%) were significantly higher than in normal gastric mucosa (11.2%), P < 0.01. Survivin expression gradually increased from 41.7% in well differentiated adenocarcinoma through 58.3% in moderately differentiated adenocarcinoma to 75.6% in poorly differentiated adenocarcinoma, with significant Rank correlation, rk = 0.279, P < 0.01. The positive rate of survivin in gastric carcinoma of diffused type (74.6%) was significantly higher than that in intestinal type (51.3%), P < 0.05. In gastric carcinoma with lymph node metastasis (76.9%), the positive rate of survivin was significantly higher than that in the group without lymph node metastasis (41.2%), P < 0.01. In 98 cases of gastric carcinoma, the expression of YAP and of survivin were positively correlated, rk = 0.246, P < 0.01.CONCLUSION: YAP may play an important role as a carcinogenic factor and may induce survivin expression. Detecting both markers together may help in early diagnosis of gastric carcinoma.
Expression of p53, inducible nitric oxide synthase and vascular endothelial growth factor in gastric precancerous and cancerous lesions: correlation with clinical features
Chang Feng, Li Wang, Lian Jiao, Bin Liu, Shu Zheng, Xin Xie
BMC Cancer , 2002, DOI: 10.1186/1471-2407-2-8
Abstract: The expression of p53, iNOS and VEGF in gastric precancerous and cancerous lesions and its relation with the clinical features was determined with immunohistochemistry (avidin-biotin-peroxidase complex method) on 55 randomly selected GC patients and 60 symptom-free subjects from the mass survey in the high-incidence area for GC in Henan, northern China.The positive immunostainig rates for p53, iNOS and VEGF in gastric carcinomas were 51%, 44% and 51%, respectively, and correlated well with TNM stages, but did not show significant difference among the groups with different degrees of gastric wall invasion depth by GC. A positive immunostaining reaction for the iNOS protein was significantly correlated with lymph node metastasis (p = 0.019; Spearman correlation coefficient). P53 protein accumulation was higher in the poorly-differentiated gastric carcinoma than in well-differentiated one. In gastric biopsies, no positive immunosatining was observed for p53, iNOS and VEGF in the histologically normal tissue and chronic superficial gastritis (CSG). However, p53, iNOS and VEGF positive immunostaining was observed in the tissues with different severities of lesions of chronic atrophic gastritis (CAG), intestinal metaplasia (IM) and dysplasia (DYS), and the positive rates increased with the lesion progression from CAG to IM to DYS. A high coincidental positive and negative immunostaining rate for p53, iNOS and VEGF was observed both in biopsy samples with CAG, IM and DYS from the symptom-free subjects and in gastric cancer tissue from the GC patients.The present results indicated that p53 protein accumulation and increased expression of iNOS and VEGF might be responsible for gastric carcinogenesis and tumor aggressiveness of gastric cancer.Gastric adenocarcinoma (GC) is one of the most common malignant diseases in China. Although the GC has been declined in its mortality rate, it remains the leading cause of cancer-related deaths in this country (Parkin et al., 1992; World
中药治疗胃癌前病变的临床研究现状
Status of Clinical Studies on Treating Precancerous Lesions of Gastric Cancer with Chinese Herbs
 [PDF]

唐人彦, 林江
Traditional Chinese Medicine (TCM) , 2014, DOI: 10.12677/TCM.2014.34015
Abstract:
我国是胃癌高发和高死亡率国家。胃癌多是从胃癌前病变逐渐演变而来的。阻止或逆转胃癌前病变是预防胃癌发生的关键措施之一。中药长期用于胃癌前病变的治疗。本文对中医对胃癌前病变病因病机的认识和对胃癌前病变常见证型临床治疗的研究现状进行综述。
China has high incidence and mortality of gastric cancer. Gastric cancer develops from precancerous lesions of gastric cancer (PLGC). Impeding or reversing the development of PLGC is one of the key measures of preventing gastric cancer. Herbal medicines have been applied to treat PLGC for a long time. This article reviews the TCM pathogenesis of PLGC and the TCM treatment on the common TCM syndromes of PLGC.
Frequency of Precancerous Lesions in Stomach of Patients
T. Khamehchian,H. Sharifi,T. Mazuchi,S.A. Talari
Journal of Medical Sciences , 2006,
Abstract: This descriptive study was done duo to determine of frequencies of precancerous lesions and its significance in gastric cancer prophylaxis and no accessible data about their in this region. From 177 patients with gastrointestinal symptoms that were selected for endoscopy, biopsies were taken from fundus, antrum, lesser curvature. 24.9% of patients had precancerous lesion that 20.9% were Intestinal metaplasia (IM) and 4% of them were dysplasia. Fifty four percent of IM was in antrum, 27.1% in fundus and 18.9% in lesser curvature. In patients with precancerous lesions the most clinical symptom was abdominal pain (29.5%) and the least common was melena (9.1%) and 52.8% of them had Helicobacter pylori (HP) infection. 39.4% of patients were female and 63.3% were male. The most common precancerous lesions were observed in the fourth decade life (29.5%) Therefore considering to high frequency of precancerous lesions in this study and its significance in gastric cancer prophylaxis, serial endoscopic studies are recommended.
Significance and relationship between Cripto-1 and p-STAT3 expression in gastric cancer and precancerous lesions  [cached]
Jian-Guo Zhang, Jing Zhao, Yan Xin
World Journal of Gastroenterology , 2010,
Abstract: AIM: To explore the relationship between Cripto-1 (CR-1) and tyrosine phosphorylation STAT3 (p-STAT3) expressions in gastric cancer (GC) and gastric carcinogensis and metastasis.METHODS: The PV9000 immunohistochemical method was used to detect the expression of CR-1 and p-STAT3 in 178 cases of GC, 95 matched normal gastric mucosa, 40 chronic atrophic gastritis (CAG), 48 intestinal metaplasia (IM) and 25 dysplasia (DYS).RESULTS: The positive rates of CR-1 and p-STAT3 expression were significantly higher in CAG (65.0% and 60.0%), in IM (83.3% and 77.1%), DYS (80.0% and 68%) and GC (71.3% and 60.1%) than in normal gastric mucosa (43.2% and 41.1%, P < 0.05), respectively. The expressions of CR-1 and p-STAT3 (78.3% and 66.7%) were significantly higher in GC with lymph node metastasis than in those without metastasis (53.1% and 42.9%, P < 0.05). CR-1 expression was also related to histological and Lauren’s types of GC (P < 0.001). Furthermore, there was positive relationship between CR-1 and p-STAT3 expressions in GC (rk = 0.189, P = 0.002).CONCLUSION: The up-regulation of CR-1 and p-STAT3 may play important roles in gastric carcinogenesis and lymph node metastasis. CR-1 and p-STAT3 expression in GC was positively correlated, and the relevant molecular mechanism requires further investigations.
Oral Precancerous Lesions in Tobacco Users attending Dental Outdoor in Jhalawar District of Rajasthan  [PDF]
Panwar Sajid K, Ansari Shakeel M, Mulla Shakila K
National Journal of Community Medicine , 2012,
Abstract: Background: Tobacco is well established risk factor for oral cancer. Different aspects of tobacco consumption like mode of consumption, duration and frequency of consumption might influence occurrence of precancerous lesions. Another factor in tobacco consumption is a€ severity of tobacco usea€ , which is not assessed routinely while history taking. Precancerous lesion is an alarm for progression towards cancer. Unfortunately awareness regarding precancerous lesion is lacking in the community. Hence the current study was done to correlate different aspects of tobacco consumption with occurrence of precancerous lesion and to know the awareness status of persons having precancerous lesion. Methodology: Cross-sectional study on sample of 240 tobacco users attending dental outdoor. Fagerstrom test for nicotine dependence and smokeless tobacco scale was used to assess severity of nicotine dependence. Results: In the current study prevalence of precancerous lesion was found to be 40%. 73% of subjects were unaware of precancerous lesion. Tobacco dependence severity score >5 (p =0.03) and >5 years duration of consumption (p =0.04) were found significant factors. Conclusion: Severity of tobacco consumption is an important determining factor in precancerous lesion. Intensive community participation is needed for screening precancerous lesions in tobacco users and running awareness program in collaboration with de-addiction services.
扶正消瘕方治疗胃癌前病变的临床疗效观察
The curative effect of Fuzhengxiaojia decoction on precancerous lesions of gastric cancer
 [PDF]

,,刘德山,,,,,李昌玲,姚诗清,,,,
- , 2018, DOI: 10.7652/jdyxb201804029
Abstract: 摘要:目的 观察扶正消瘕方治疗胃癌前病变(precancerous lesion of gastric cancer, PLGC)的疗效。方法 选择我院PLGC患者44例,随机分为对照组和治疗组。对照组给予胃复春片口服4片/次,3次/d;治疗组在对照组基础上加服扶正消瘕方加减每日1剂,水煎服400mL。1个月为1个疗程,连服2个疗程。结果 2组治疗前的超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malondialdehyde, MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)、IgG、IgM和IgA无统计学差异(P>0.05)。治疗后,与对照组相比,治疗组的SOD(t=2.144,P=0.044)和GSH-Px(t=2.322,P=0.030)升高,MDA(t=3.096,P=0.005)、IgG(t=2.421,P=0.025)、IgM(t=3.377,P=0.003)和IgA(t=2.521,P=0.020)降低。治疗前2组中医主要症状积分无统计学差异(P>0.05);治疗后,与对照组相比,治疗组的中医主要症状饮食减少(t=3.924,P<0.001)、胃中嘈杂(t=4.161,P<0.001)和胃脘或胁肋胀(t=2.881,P<0.009)积分降低,总有效率增高(χ2=4.539,P=0.033)。结论 胃复春片联合扶正消瘕方加减治疗PLGC较单纯中成药治疗有更好的疗效,与改善氧化还原及免疫球蛋白有关。
ABSTRACT: Objective To observe the curative effect of Fuzhengxiaojia decoction on precancerous lesion of gastric cancer (PLGC). Methods We randomly divided 44 patients with PLGC in our hospital into control group (n=22) and treatment group (n=22). The control group was given 4 Weifuchun tablets each time and three times per day and while the treatment group was given one Fuzhengxiaojia decoction of 400mL besides the medication of the control group. They were treated for two courses, one course lasting for one month. Results Superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), IgG, IgM and IgA in the two groups had no significant differences before treatment (P>0.05). After treatment, compared with those in the control group, SOD (t=2.144, P=0.044) and GSH-Px (t=2.322, P=0.030) increased, while MDA(t=3.096, P=0.005), IgG(t=2.421, P=0.025), IgM(t=3.377, P=0.003) and IgA (t=2.521, P=0.020) decreased. The main symptom scores in the two groups did not significantly differ before treatment (P<0.05). After treatment, compared with those in the control group, the scores for main symptoms like reduced food intake (t=3.924, P<0.001), stomach noise (t=4.161, P<0.001) and gastric or hypochondriac swelling (t=2.881, P<0.009) decreased in the treatment group. The rate of effective cases was higher than that in the control group (χ2=4.539, P=0.033). Conclusion The effect of Weifuchun combined with Fuzhengxiaojia prescriptions in treating PLGC is better than Weifuchun alone, which is related to improving redox and immunoglobulin
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