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EVALUASI IMUNOSEROLOGI PADA BAYI PASCA IMUNISASI HEPATITIS B LENGKAP  [cached]
Muljati Prijanto,Sarwo Handayani,Bambang Herijanto,Farida S. Sumarno
Bulletin of Health Research , 2012,
Abstract: EVALUASI IMUNOSEROLOGI PADA BAYI PASCA IMUNISASI HEPATITIS B LENGKAP
PENYUSUNAN PEDOMAN EVALUASI MANAJEMEN BIAYA OBAT DAN BIAYA RIIL PENGOBATAN  [cached]
Sriana Azis,Rini Sasanti Handayani,Max Joseph Herman
Bulletin of Health Research , 2012,
Abstract: PENYUSUNAN PEDOMAN EVALUASI MANAJEMEN BIAYA OBAT DAN BIAYA RIIL PENGOBATAN
Field evaluation of a rapid immunochromatographic dipstick test for the diagnosis of cholera in a high-risk population
Xuan-Yi Wang, M Ansaruzzaman, Raul Vaz, Catarina Mondlane, Marcelino ES Lucas, Lorenz von Seidlein, Jacqueline L Deen, Sonia Ampuero, Mahesh Puri, Taesung Park, GB Nair, John D Clemens, Claire-Lise Chaignat, Minoarisoa Rajerison, Farida Nato, Jean-Michel Fournier
BMC Infectious Diseases , 2006, DOI: 10.1186/1471-2334-6-17
Abstract: The performance of an immunochromatographic dipstick test (Institute Pasteur, Paris, France) specific for Vibrio cholerae O1 was evaluated in a prospective study in Beira, Mozambique, during the 2004 cholera season (January-May). Fecal specimens were collected from 391 patients with acute watery nonbloody diarrhea and tested by dipstick and conventional culture.The overall sensitivity and specificity of the rapid test compared to culture were 95% (95% confidence interval [CI]: 91%–99%) and 89% (95% CI: 86%–93%), respectively. After stratification by type of sample (rectal swab/bulk stool) and severity of diarrhea, the sensitivity ranged between 85% and 98% and specificity between 77% and 97%.This one-step dipstick test performed well in the diagnosis of V. cholerae O1 in a setting with seasonal outbreaks where rapid tests are most urgently needed.The cardinal clinical feature of cholera is a severe dehydrating diarrhea, which can lead to severe and rapidly progressing dehydration and shock. Despite advances in the understanding of its pathophysiology and transmission, cholera remains a major international health concern. In 2003, the World Health Organization (WHO) received reports from 45 countries of 11,575 cholera cases and 1,894 related deaths. The majority of cholera cases occurred in sub-Saharan Africa [1]. However, these numbers are considered gross underestimates since outbreaks are often not reported due to fear of travel and trade sanctions. Critical interventions for cholera control include improved access to efficient treatment facilities, education to promote good personal hygiene, and improvement of sanitation and safe water supply [2-4]. But successful interventions depend on early detection of cholera outbreaks. Therefore, an efficient cholera surveillance system should be a high priority in the control of cholera [1,5].The conventional culture methods currently used for diagnosis of Vibrio cholerae remain the gold standard but require a functioning
Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus
Chen Keyan,Zhao Kui,Song Deguang,He Wenqi
Virology Journal , 2012, DOI: 10.1186/1743-422x-9-172
Abstract: Background The incidence of PHE among pigs in many countries is on the rise, and it has caused great economic losses to the pig industry. Therefore, the development of a sensitive, specific, and easily-performed assay is crucial for the rapid detection and surveillance of PHE-CoV infection and transmission. Results An immunochromatographic strip was developed for the detection of PHE-CoV. The colloidal gold-labeled MAb 4D4 was used as the detection reagent, and the MAb 1E2 and goat anti-mouse IgG coated the strip's test and control lines, respectively. The immunochromatographic strip was capable of specifically detecting PHE-CoV with a HA unit of 2 within 10 min. Storage of the strips at room temperature for 6 months or at 4°C for 12 months did not change their sensitivity or specificity. Using RT-PCR as a reference test, the relative specificity and sensitivity of the immunochromatographic strip were determined to be 100% and 97.78%, respectively. There was an excellent agreement between the results obtained by RT-PCR and the immunochromatographic strips (kappa = 0.976). Additionally, there was a strong agreement between the sandwich enzyme-linked immunosorbent assay (ELISA) and immunochromatographic strips (Kappa = 0.976). When the immunochromatographic strips were used for diagnosing PHE-CoV infection in the Jilin Province, the PHE-CoV-positive rate ranged from 61.54% in the Jilin district to 17.95% in the Songyuan district. Conclusions Based on its high specificity, sensitivity, and stability, the immunochromatographic strip would be suitable for on-site detection of PHE-CoV for surveillance and epidemiological purposes.
PENYUSUNAN PEDOMAN EVALUASI PENGELOLAAN DAN PEMBIAYAAN OBAT BERDASARKAN PENGUKURAN INDIKATOR DI PUSKESMAS KABUPATEN PEKALONGAN DARI TAHUN 1995--1999  [cached]
Sriana Azis,Sri Endreswari,Daroham Mutiatikum,Pudji Lastari
Bulletin of Health Research , 2012,
Abstract: PENYUSUNAN PEDOMAN EVALUASI PENGELOLAAN DAN PEMBIAYAAN OBAT BERDASARKAN PENGUKURAN INDIKATOR DI PUSKESMAS KABUPATEN PEKALONGAN DARI TAHUN 1995--1999
Preliminary observations on fluids incubated with Wuchereria bancrofti using the immunochromatographic test
Dreyer, Gerusa;Nor?es, Joaquim;Lanfredi, Reinalda;Lins, Renato;Oliveira-Menezes, Aleksandra;Figueredo-Silva, José;
Revista da Sociedade Brasileira de Medicina Tropical , 2008, DOI: 10.1590/S0037-86822008000200017
Abstract: to assess the performance of the immunochromatographic test for filariasis, adult wuchereria bancrofti worms were incubated under different conditions. the tests were strongly positive with incubation fluids from both living and mechanically damaged females. negative results were observed with incubation fluids from all male worms and from intact dead females.
A Novel Image Methodology for Interpretation of Gold Immunochromatographic Strip  [cached]
Yurong Li,Nianyin Zeng,Min Du
Journal of Computers , 2011, DOI: 10.4304/jcp.6.3.540-547
Abstract: Gold immunochromatographic strip assay is a rapid, simple, single-copy and on-site method. Quantitative Interpretation of the strip can provide more information than the traditional qualitative or semiquantitative strip assay. The paper aims to develop an image based assay method for quantitative determination of trace concentrations by gold immunochromatographic strip. The image of gold immunochromatographic strip is taken by CCD, and, after the proper filter and window cutting, the test line and control line is segmented by the genetic fast fuzzy c-means(FCM) clustering algorithm. In order to improve the measure property, based on Lambert-beer law, the relative reflective integral optical density(RIOD) is selected as the feature by which the interference in the test and control lines can be canceled out each other. The proposed method is applied to the quantitative detection of human chorionic gonadotropin (hCG) as a model. Firstly, the segmentation performance of the genetic fast FCM clustering algorithm is compared with threshold method and FCM clustering algorithm in terms of the peak signal-to-noise ratio (PSNR). Furthermore, the comparison of the blind experiment between the proposed method and commercial quantitative instrument swp-sc1 is carried out. This method is shown to deliver a result comparable and even superior to existing techniques.
IMMUNOCHROMATOGRAPHIC TEST OVER MICROSCOPY IN DIAGNOSIS OF SEVERE MALARIA IN BANGLADESH  [cached]
Dr Anisul Awwal
Journal of Armed Forces Medical College, Bangladesh , 2011,
Abstract: Objective: This study was conducted to determine theadvantage of Immunochromatographic test overmicroscopic peripheral smear in clinically suspectedsevere malaria.Methods: Total 106 consecutive clinically diagnosedsevere malaria cases were enrolled with patients over12 years of age irrespective of sex, race, religion andpregnancy from March to August 2002, at onemedicine unit of Chittagong Medical CollegeHospital. The immunochromatographic rapiddiagnosis test and microscopy were performed in allcases.Results: Sensitivity and specificity ofimmunochromatographic Paracheck Pf test wasfound 91.42% and 90.65% in this study. Positivepredictive value was 90.56% and negative predictivevalue was 91.5%. The sensitivity of microscopy wasonly 69.8%.Conclusion: Reliable quality controlled malariamicroscopy is difficult to provide in many ruralremote areas due to lack of logistics manpower andelectricity facility. Rapid diagnostic test likeParacheck Pf test could be of use in such a situationwhich is reliable, cheap and easy to perform.
Validation of an immunochromatographic assay kit for the identification of the Mycobacterium tuberculosis complex
Toihir, Al-Habib Omar Said;Rasolofo, Voahangy;Andrianarisoa, Samuel Hermas;Ranjalahy, Gabriel Marie;Ramarokoto, Herimanana;
Memórias do Instituto Oswaldo Cruz , 2011, DOI: 10.1590/S0074-02762011000600022
Abstract: the performance of the immunochromatographic assay, sd bioline tb ag mpt64 rapid?, was evaluated in madagascar. using mouse anti-mpt64 monoclonal antibodies for rapid discrimination between the mycobacterium tuberculosis complex and nontuberculous mycobacteria, the kit was tested on mycobacteria and other pathogens using conventional methods as the gold standard. the results presented here indicate that this kit has excellent sensitivity (100%) and specificity (100%) compared to standard biochemical detection and can be easily used for the rapid identification of m. tuberculosis complex.
Preparation of Colloidal Gold Immunochromatographic Strip for Detection of Paragonimiasis skrjabini  [PDF]
Ying Wang, Lifang Wang, Jianwei Zhang, Guangxi Wang, Wenbi Chen, Lin Chen, Xilin Zhang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0092034
Abstract: Background Paragonimiasis is a food-borne trematodiasis, a serious public health issue and a neglected tropical disease. Paragonimus skrjabini is a unique species found in China. Unlike paragonimiasis westermani, it is nearly impossible to make a definitive diagnosis for paragonimiasis skrjabini by finding eggs in sputum or feces. Immunodiagnosis is the best choice to detect paragonimiasis skrjabini. There is an urgent need to develop a novel, rapid and simple immunoassay for large-scale screening patients in endemic areas. Methodology/Principal Findings To develop a rapid, simple immunodiagnostic assay for paragonimiasis, rabbit anti-human IgG was conjugated to colloidal gold particles and used to detect antibodies in the sera of paragonimiasis patients. The synthesis and identification of colloidal gold particles and antibody-colloidal gold conjugates were performed. The size of colloidal gold particles was examined using a transmission electron microscope (TEM). The average diameter of colloidal gold particles was 17.46 nm with a range of 14.32–21.80 nm according to the TEM images. The formation of antibody-colloidal gold conjugates was monitored by UV/Vis spectroscopy. Excretory-secretory (ES) antigen of Paragonimus skrjabini was coated on nitrocellulose membrane as the capture line. Recombinant Staphylococcus protein A was used to prepare the control line. This rapid gold immunochromatographic strip was assembled in regular sequence through different accessories sticked on PVC board. The relative sensitivity and specificity of the strip was 94.4% (51/54) and 94.1% (32/34) respectively using ELISA as the standard method. Its stability and reproducibility were quite excellent after storage of the strip at 4°C for 6 months. Conclusions/Significance Immunochromatographic strip prepared in this study can be used in a rapid one-step immunochromatographic assay, which is instantaneous and convenient.
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