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Composition of dental plaque formed in the presence of sucrose and after its interruption
Cury, Jaime Aparecido;Marques, Aline Soler;Tabchoury, Cíntia Pereira Machado;Del Bel Cury, Altair Antoninha;
Brazilian Dental Journal , 2003, DOI: 10.1590/S0103-64402003000300001
Abstract: since dental plaque reservoirs of fluoride (f), calcium (ca) and inorganic phosphorus (pi) are susceptible to decreases in ph, this in situ crossover study was conducted to test the hypothesis that the low concentration of these ions in plaque, formed in the presence of sucrose, could be attributed merely to the fermentation of this sugar. eleven volunteers wore palatal appliances containing 6 human enamel blocks during two stages. in each stage, the treatments were either 20% sucrose solution or distilled deionized water, which were dripped onto the blocks 8 times a day. after 28 days, in each stage, the dental plaque formed on two blocks was collected, the treatment was inverted and after a further 24 and 48 h, the biofilm formed was collected from the other blocks. the concentration of acid-soluble f, ca and pi, and the concentration of insoluble polysaccharide (ip) were determined in the dental plaque. statistically lower concentrations of f, ca and pi, and a higher concentration of ip were found in the 28-day biofilm formed in the presence of sucrose than in its absence; after the treatment inversion the change in f, ca and pi was not statistically significant, but the ip concentration changed significantly. the hypothesis was rejected because change in concentration of f, ca and pi is not due to fermentation of the sucrose.
Composition of dental plaque formed in the presence of sucrose and after its interruption  [cached]
Cury Jaime Aparecido,Marques Aline Soler,Tabchoury Cíntia Pereira Machado,Del Bel Cury Altair Antoninha
Brazilian Dental Journal , 2003,
Abstract: Since dental plaque reservoirs of fluoride (F), calcium (Ca) and inorganic phosphorus (Pi) are susceptible to decreases in pH, this in situ crossover study was conducted to test the hypothesis that the low concentration of these ions in plaque, formed in the presence of sucrose, could be attributed merely to the fermentation of this sugar. Eleven volunteers wore palatal appliances containing 6 human enamel blocks during two stages. In each stage, the treatments were either 20% sucrose solution or distilled deionized water, which were dripped onto the blocks 8 times a day. After 28 days, in each stage, the dental plaque formed on two blocks was collected, the treatment was inverted and after a further 24 and 48 h, the biofilm formed was collected from the other blocks. The concentration of acid-soluble F, Ca and Pi, and the concentration of insoluble polysaccharide (IP) were determined in the dental plaque. Statistically lower concentrations of F, Ca and Pi, and a higher concentration of IP were found in the 28-day biofilm formed in the presence of sucrose than in its absence; after the treatment inversion the change in F, Ca and Pi was not statistically significant, but the IP concentration changed significantly. The hypothesis was rejected because change in concentration of F, Ca and Pi is not due to fermentation of the sucrose.
A study of influence of sugars on the modulations of dental plaque pH in children with rampant caries, moderate caries and no caries  [cached]
Utreja D,Tewari A,Chawla H
Journal of the Indian Society of Pedodontics and Preventive Dentistry , 2010,
Abstract: The present study is undertaken to find out the pH of resting plaque in children with no caries, moderate caries and rampant caries and to determine the modulations of plaque pH with different sugar solution rinses viz: sucrose, glucose and fructose. The study was carried out on forty five children, in the age group of 3-10 years (25 males and 20 females). The child was given 10 ml of test solution and was asked to rinse and swish it in the mouth for a period of 30 sec. Plaque samples were taken from 20 different spots after 5, 10, 20 and 30 min of the rinse and pH values of all the samples were determined. Results show that there was a statistically significant (P<0.05) difference between the pH values of plaque at different intervals of time with sucrose, fructose and glucose solution rinse in children with moderate caries, rampant caries as compared to the caries free group. Sucrose was found to be highly cariogenic in all the children with a greater potentiating effect in moderate and rampant caries. Glucose also appeared to have a cariogenic role while fructose had the least of it all.
Sucrose concentration and pH in liquid oral pediatric medicines of long-term use for children
Passos,Isabela Albuquerque; Sampaio,Fábio Correia; Martínez,Cosme Rafael; Freitas,Cláudia Helena Soares de Morais;
Revista Panamericana de Salud Pública , 2010, DOI: 10.1590/S1020-49892010000200007
Abstract: objectives: to determine the ph and sucrose concentrations (sc) of pediatric liquid drugs of long-term use by children in order to evaluate the potential risk for dental caries and dental erosion. methods: after assessing the ph, we analyzed 71 aqueous medicine samples for sucrose by the lane-eynon general volumetric method. the ph and sc values (mean ± standard deviation (sd) were calculated according to therapeutic action. results: the highest and the lowest sc values (mean ± sd) were found in respiratory (37.75% ± 17.23%) and endocrine drugs (11.97% ± 15.16%) (p < 0.01). the values for medicines prescribed for daily ingestion were 47.15% ± 9.57%, whereas for twice daily and three or four times a day, these numbers were 24.42% ± 18.03% and 34.43% ± 14.83%, respectively (p < 0.01). the sc (mean ± sd)) values were higher in syrups (36.32% ± 17.62%) than in other formulations (p > 0.05). the overall ph (mean ± sd) was 5.89 ± 2.02 (range 2.3 ± 0.01 to 10.6 ± 0.02). in products with acidic ph, the sc (mean ± sd) was significantly lower (22.14% ± 15.72%) than in nonacidic medicines (39.22% ± 15.82%) (p < 0.001). conclusions: it can be concluded that the pediatric medicines studied have a high sc and low ph, which vary according to therapeutic class, daily dose, and brand. caution about dental caries, dental erosion, and systemic diseases such as diabetes mellitus is warranted when these medicines are ingested frequently.
Evaluation of acidity and total sugar content of children′s popular beverages and their effect on plaque pH  [cached]
Saeed S,Al-Tinawi M
Journal of the Indian Society of Pedodontics and Preventive Dentistry , 2010,
Abstract: Background: In the developing countries, dental caries has increased with the increased exposure to dietary sugars. There is no data on the cariogenicity and acidogenicity of popular beverages in the Syrian market. Aims: To investigate the endogenous pH, titratable acidity, and total sugar content of popular beverages (cola, orange juice, and full-fat milk), and assess plaque pH drop after consumption. Settings and Design: Twenty-five healthy children with a mean age of 11.8 ± 0.6 years were recruited for this single blinded study. Materials and Methods: The pH of beverages was measured by a digital pH meter and the titratable acidity was expressed as the volume of 0.1 N sodium hydroxide required to neutralize the beverage. The total sugar content was estimated using High Performance Liquid Chromatography. Children rinsed with 15 mL of each of the beverages for 1 min. The controls used were 10% sucrose and 10% sorbitol solutions. The plaque pH was assessed before and after rinsing. Statistical Analysis: Statistical analysis was performed using analysis of variance followed by Bonferroni test to assess minimum pH, maximum pH drop, and the area under curve. P value was set as 0.05. Results: Both cola and orange juice had a low pH and similar total sugar content in contrast to the high pH and low sugar content of milk. Cola and orange juice were not statistically different from 10% sucrose (P > 0.05), but different from 10% sorbitol (P < 0.05), in contrast to milk, for all the parameters studied. Conclusion: Cola and orange juice are cariogenic/acidogenic and frequent intake should be discouraged. A reasonable intake of unsweetened milk may be advised safely.
Inverse pH Regulation of Plant and Fungal Sucrose Transporters: A Mechanism to Regulate Competition for Sucrose at the Host/Pathogen Interface?  [PDF]
Kathrin Wippel,Anke Wittek,Rainer Hedrich,Norbert Sauer
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0012429
Abstract: Plant sucrose transporter activities were shown to respond to changes in the extracellular pH and redox status, and oxidizing compounds like glutathione (GSSG) or H2O2 were reported to effect the subcellular targeting of these proteins. We hypothesized that changes in both parameters might be used to modulate the activities of competing sucrose transporters at a plant/pathogen interface. We, therefore, compared the effects of redox-active compounds and of extracellular pH on the sucrose transporters UmSRT1 and ZmSUT1 known to compete for extracellular sucrose in the Ustilago maydis (corn smut)/Zea mays (maize) pathosystem.
In vitro Shoot Multiplication of Chrysanthemum morifolium as Affected by Sucrose, Agar and pH  [PDF]
M.Z. Karim,M.N. Amin,M.A.K. Azad,F. Begum
Biotechnology , 2003,
Abstract: Effects of sucrose, agar and pH on in vitro shoot multiplication of Chrysanthemum were studied. Nodal explant from the ex vitro grown plant was used as the test material. For optimum shoot induction and multiplication in MS medium containing BAP+ sucrose 30 gm l-1, agar 6 gm l-1 and pH 5.5-6.0 proved more effective. The media having 30 gm l-1 sucrose showed the highest percentage of explant responded to shoot proliferation and that was 100%. This sucrose concentration also showed the optimum result for number of usable shoots per culture, number of node shoot 1 and average length of shoots and the values were 5.4?0.6, 5.1?0.8 and 5.6?0.4 cm. The highest proliferation response of the explant was observed on MS medium having 6 gm l-1 of agar and the frequency was 100%. Among different level of pH, the highest percentage of explant showing proliferation was observed on the media adjusted to pH 5.5 and 6.0. The results presented here proved to be suitable for the in vitro shoot multiplication of Chrysanthemum morifolium.
A study on the pH of commonly used soaps/cleansers available in the Indian market  [cached]
Tyebkhan Gulrez
Indian Journal of Dermatology, Venereology and Leprology , 2001,
Abstract: Cleansers, commonly referred to as soaps (though all soaps are cleansers, all cleansers are not soaps), are an important adjuvant in the treatment of any dermatological condition. In order to recommend a cleanser, many aspect should be taken into consideration one important being its pH. pH of a cleanser tends to have an impact on the skin in several ways viz. alteration of bacterial flora and pH, moisture content and irritation. A study was conducted to evaluate the pH of various commonly available soaps/cleansers available in the Indian market. Most of the commonly used soaps have an alkaline pH.
Relationship between Cariogenic Bacteria and pH of Dental Plaque at Margin of Fixed Prostheses
Junko Tanaka,Norio Mukai,Muto Tanaka,Masahiro Tanaka
International Journal of Dentistry , 2012, DOI: 10.1155/2012/452108
Abstract: Objective. The purpose of this study was to investigate whether teeth that have undergone prosthetic restoration are under conditions that promote caries recurrence. Methods. The subjects were 20 dentate adults with both a healthy tooth and an affected tooth entirely covered with a complete cast crown in the molar regions of the same arch. The pH was measured in plaque adhering to the margin of the tooth covered with a complete cast crown and adhering to the cervicobuccal area of the natural tooth. In addition, the numbers of cariogenic bacteria (mutans streptococci and lactobacilli) were measured employing the saliva test. The relationships between the number of cariogenic bacteria and plaque pH of the natural tooth and between the number of cariogenic bacteria and plaque pH of the tooth covered with a complete cast crown were investigated. Results. The plaque pH of the tooth covered with a complete cast crown decreased as the numbers of SM and LB increased. The natural tooth were also influenced by the number of SM. Conclusion. Secondary caries are likely to develop from the marginal region of the crown in the oral cavity with a high caries risk unless a preventive program is prepared and the oral environment is improved following the program.
Effects of the Sucrose Concentration and pH Level on Shoot Regeneration from Callus in Araria elata Seem  [PDF]
M.Z. Karim,S. Yokota,M.M. Rahman,J. Eizawa
Asian Journal of Plant Sciences , 2007,
Abstract: The optimum sucrose concentration and pH level were estimated for in vitro shoot regeneration from callus of Aralia elata Seem in a Broad-leaved tree (BT) medium with different concentrations of sucrose (0.0-25.0 g L-1) and a wide range of pH levels (4.5-6.8). The highest rate of shoot production was achieved in a shoot-regenerating medium with 15 g L-1 sucrose and a pH of 5.8. Shoot multiplication and growth were significantly affected by sucrose. Shoots were not formed in a sucrose-free medium. When the pH was adjusted below or above 5.8, the growth rate of the shoot significantly decreased.
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