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Simultaneous and Rapid Determination of Main Lignans in Different Parts of Schisandra Sphenanthera by Micellar Electrokinetic Capillary Chromatography  [PDF]
Guangxin Yuan,Yang Liu,Tan Li,Yan Wang,Yu Sheng,Ming Guan
Molecules , 2011, DOI: 10.3390/molecules16053713
Abstract: Lignans are imporant active ingredients of Schisandra sphenanthera. A micellar electrokinetic chromatography method was developed for the simultaneous determination of eight lignans – schizandrin, schisandrol B, schisantherin A, schisanhenol, anwulignan, deoxyschizandrin, schizandrin B and schizandrin C – in different parts of S. sphenanthera. The key factors for separation and determination were studied and the best analysis conditions were obtained using a background electrolyte of 10 mM phosphate-37.5 mM SDS-35% v/v acetonitrile (pH 8.0) at the separation voltage of 28 kV and detection at 214 nm, whereby the plant samples could be analyzed within 9.0 min. Analysis yielded good reproducibility (RSD between 1.19-2.28%) and good recovery (between 92.2-103.8%). The detection limits (LOD) and limit of quantification (LOQ) were within 0.4-1.2 mg/L and 1.5-4.0 mg/L. This method is promising to improve the quality control of different parts of S. sphenanthera.
Population genetic diversity and structure of Schisandra sphenanthera, a medicinal plant in China
药用植物华中五味子的种群遗传多样性及遗传结构

YAN Bo-qian,WANG Ting,HU Li-le,
闫伯前
,王艇,胡理乐

生态学杂志 , 2009,
Abstract: 华中五味子(Schisandra sphenanthera)是著名的药用植物,具有悠久的药用历史和巨大的开发潜力。为了有效评估、利用和保护华中五味子资源,应用自主开发的9对SSR引物研究了华中五味子自然种群的遗传多样性与遗传结构。结果表明:在10个采样种群中,共检测到58个等位基因,平均预期杂合度HE为0.528,平均观察杂合度HO为0.519,较大的连续种群保持了较高的遗传多样性,而小种群的遗传多样性则相对较低;华中五味子总体表现为显著的杂合子缺失,内繁育系数FIS为0.042;种群间总的遗传分化系数FST为0.108,两两种群间分化显著;贝叶斯聚类结果把10个采样种群按遗传组成分为江南组和江北组2组,长江所形成的特殊地理屏障对华中五味子江南和江北地区间较高的遗传分化造成了影响。
Antioxidant isolated from Schisandra propinqua (Wall.) Baill
HUANG,FENG; XU,LIJIA; GANGGANG,SHI;
Biological Research , 2009, DOI: 10.4067/S0716-97602009000300009
Abstract: schisandra propinqua (wall.) baill.(schisandraceae) is widely used as a chinese folk medicine. in this study, activity-guided fractionation of the ethanol extract from the stem of schisandra propinqua led to the isolation of four extracts. subsequently, a neolignan 4,4-di(4-hydroxy-3-methoxyphenly)-2,3-dimethylbutanol was isolated from the etoac part of the stem of schisandra propinqua, the free radical scavenging activities of which were researched in vitro. the present work demonstrated that extracts and pure compound possessed scavenging activities to dpph, superoxide anions and hydroxy radical, and could depress lipid peroxidation reaction induced by oxygen radical produced by the fe2+/cysteine system in vitro. this suggests that the traditional application of schisandra propinqua in china may be related to its antioxidant activities, and the etoac part of the stems of schisandra propinqua can be utilized as an effective source of antioxidants.
Antioxidant isolated from Schisandra propinqua (Wall.) Baill  [cached]
FENG HUANG,LIJIA XU,SHI GANGGANG
Biological Research , 2009,
Abstract: Schisandra propinqua (Wall.) Baill.(Schisandraceae) is widely used as a Chinese folk medicine. In this study, activity-guided fractionation of the ethanol extract from the stem of Schisandra propinqua led to the isolation of four extracts. Subsequently, a neolignan 4,4-di(4-hydroxy-3-methoxyphenly)-2,3-dimethylbutanol was isolated from the EtOAc part of the stem of Schisandra propinqua, the free radical scavenging activities of which were researched in vitro. The present work demonstrated that extracts and pure compound possessed scavenging activities to DPPH, superoxide anions and hydroxy radical, and could depress lipid peroxidation reaction induced by oxygen radical produced by the Fe2+/cysteine system in vitro. This suggests that the traditional application of Schisandra propinqua in China may be related to its antioxidant activities, and the EtOAc part of the stems of Schisandra propinqua can be utilized as an effective source of antioxidants.
Serum Levels of Acylcarnitines Are Altered in Prediabetic Conditions  [PDF]
Manuel Mai, Anke T?njes, Peter Kovacs, Michael Stumvoll, Georg Martin Fiedler, Alexander Benedikt Leichtle
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0082459
Abstract: Objective The role of mitochondrial function in the complex pathogenesis of type 2 diabetes is not yet completely understood. Therefore, the aim of this study was to investigate serum concentrations of short-, medium- and long-chain acylcarnitines as markers of mitochondrial function in volunteers with normal, impaired or diabetic glucose control. Methods Based on a 75 g oral glucose tolerance test, 1019 studied subjects were divided into a group with normal glucose tolerance (NGT; n = 636), isolated impaired fasting glycaemia (IFG; n = 184), impaired glucose tolerance (IGT; n = 87) or type 2 diabetes (T2D; n = 112). Serum concentrations of free carnitine and 24 acylcarnitines were measured by mass spectrometry. Results Serum levels of acetylcarnitine (C2), propionylcarnitine (C3), octanoylcarnitine (C8), malonylcarnitine/hydroxybutyrylcarnitine (C3DC+C4OH), hexanoylcarnitine (C6), octenoylcarnitine (C8:1), decanoylcarnitine (C10), decenoylcarnitine (C10:1), dodecanoylcarnitine (C12), tetradecenoylcarnitine (C14:1), tetradecadienylcarnitine (C14:2), hydroxytetradecanoylcarnitine (C14OH), hydroxyhexadecanoylcarnitine (C16OH) and octadecenoylcarnitine (C18:1) were significantly different among the groups (all p<0.05 adjusted for age, gender and BMI). Between the prediabetic states C14:1, C14:2 and C18:1 showed significantly higher serum concentrations in persons with IGT (p<0.05). Compared to T2D the IFG and the IGT subjects showed lower serum concentrations of malonylcarnitine/hydroxybutyrylcarnitine (C3DC+C4OH) (p<0.05). Conclusion Alterations in serum concentrations of several acylcarnitines, in particular tetradecenoylcarnitine (C14:1), tetradecadienylcarnitine (C14:2), octadecenoylcarnitine (C18:1) and malonylcarnitine/hydroxybutyrylcarnitine (C3DC+C4OH) are associated not only with T2D but also with prediabetic states.
THE BIOLOGY OF THE PROPAGATION OF SPECIES SCHISANDRA CHINENSIS (TURCZ.) BAILL.  [PDF]
CIORCHIN? NINA,ONICA ELISAVETA,RO?CA ION1,DUMITRA? ADELINA
Journal of Plant Development , 2011,
Abstract: The paper presents aspects regarding the possibilities for the propagation of species Schisandra chinensis (Turz.) Baill, as well as its reaction in the pedo-climatic conditions of the Republic of Moldova. Situated in the Lianarium of the Botanical Garden (Institute) A M since 1975, Schisandra chinensis (Turcz.) Baill. grows, develops and fructifies abundantly every year. It is propagated vegetatively and generatively with some difficulty. In the case of generative propagation, in order to obtain a high germination percentage, the seeds are stratified in three phases, at different temperatures and are sown in spring. Germination percentages of 80-90% were obtained. Schisandra chinensis is also propagated by greenwood cuttings, semi-hardwood or hardwood cuttings, by layering or by division. The best results were obtained by using semi-hardwood and hardwood cuttings taken in summer, in June-July, from younger plants. The potential for in vitro propagation of this species was also tested. The explants consisting of apical meristems inoculated on MS medium + 0.5 mg/l BAP evolved the best.
Evaluation on the combined effect of Sesamin and Schisandra extract on blood fluidity  [cached]
Daniel Tsi,Amabel Tan
Bioinformation , 2008,
Abstract: Several studies have demonstrated a link between blood viscosity and various forms of liver dysfunction. Therefore, we investigated the effect of liver protective herbal materials, Sesamin combined with extract of Schisandra chinensis berry (Schisandra) for its potential to improve blood fluidity in humans. Ten human subjects were recruited to study the effect of sesamin combined with schisandra extract (SCH) for two weeks on blood viscosity. Blood fluidity was measured as the transit time for 100μl of heparinized whole blood to pass through a micro-channel array setup at baseline, 1 week and 2 weeks. For safety assessment, blood biochemistry, hematology and urine analysis were taken at baseline, 1 week and 2 weeks after SCH administration. No safety concern and adverse effects were observed during the 2-week continuous intake period. Intake of SCH reduced blood passage time by 9.0% and 9.7% at 1 and 2 weeks, respectively. In conclusion, this pilot clinical study indicates that the combined administration of sesamin with schisandra extract could improve blood fluidity after 1 week of oral intake and this effect was sustained up to 2 weeks.
Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions
Osorio,José Henry; Pourfarzam,Morteza;
Colombia Médica , 2010,
Abstract: objective: to evaluate the percentage of carnitine and acylcarnitines remaining in red blood cells after washing them with different concentrations of saline solution. materials and methods: human blood samples were centrifuged and the blood cells were washed with different saline solutions. the final pellet was resuspended in pbs for card preparation and tandem mass spectrometry analysis. results: it was found that carnitine, as well as short-chain, medium-chain, and long-chain acylcarnitines remain in red blood cells at average percentages of 19.3; 34; 34; and 32%, respectively. significant differences were found for carnitine and acylcarnitine levels in blood washed with an isotonic solution compared to their levels using several hypotonic solutions (p<0.05). conclusion: because carnitine and acylcarnitines remained associated with the blood cells, we recommend using whole blood to measure these metabolites.
Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions
José Henry Osorio,Morteza Pourfarzam
Colombia Médica , 2010,
Abstract: Objective: To evaluate the percentage of carnitine and acylcarnitines remaining in red blood cells after washing them with different concentrations of saline solution.Materials and methods: Human blood samples were centrifuged and the blood cells were washed with different saline solutions. The final pellet was resuspended in PBS for card preparation and tandem mass spectrometry analysis.Results: It was found that carnitine, as well as short-chain, medium-chain, and long-chain acylcarnitines remain in red blood cells at average percentages of 19.3; 34; 34; and 32%, respectively. Significant differences were found for carnitine and acylcarnitine levels in blood washed with an isotonic solution compared to their levels using several hypotonic solutions (p<0.05).Conclusion: Because carnitine and acylcarnitines remained associated with the blood cells, we recommend using whole blood to measure these metabolites.
EFFECT OF ACETONITRILE CONCENTRATION ON ACYLCARNITINES MEASUREMENT BY TANDEM MASS SPECTROMETRY
Osorio,José Henry;
Biosalud , 2010,
Abstract: background: the several steps for acylcarnitine analysis by tandem mass spectrometry such as extraction, derivatisation, injection and others, can be influenced by some technical factors improving or impairing the levels of detection. objective: the present study evaluated the effect of different concentrations of the acetonitrile used during blood acylcarnitines measurement on the sensitivity obtained during the analysis. methodology: prior to acylcarnitine analysis by tandem mass spectrometry, samples were re-dissolved in different acetonitrile in water dilutions [50, 60, 70, 80, 90, and 100% (v/v)] and each single blood specimen was processed five times for each dilution. results: it was observed that by increasing the concentration of acetonitrile the intensity of acylcarnitine peaks significantly increased. however at the highest concentrations of acetonitrile, this reacted with the polystyrene material of the microtitre plates, and resulted in peaks appearing in acylcarnitine profiles, specifically at m/z 302. conclusion: a mixture of 70% v/v acetonitrile/water is recommended to use as optimum solvent to dissolve the extracts prior to blood acylcarnitine analysis by tandem mass spectrometry.
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