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ESSENTIAL OIL FROM MARCHANTIA CONVOLUTA: EXTRACTION AND COMPONENTS
CAO,HUI; JUAN JI,MEI; WANG,HONG XIAN;
Journal of the Chilean Chemical Society , 2007, DOI: 10.4067/S0717-97072007000100007
Abstract: the essential oil of marchantia convoluta was obtained by supercritical fluid extraction (sfe) using methanol as a modifier. the effects of different parameters, such as pressure, temperature, modifier volume and extraction time, on the extraction of essential oil from m. convoluta were investigated. maximum global yields were obtained using the following conditions: extraction temperature, 40 oc; dynamic time, 40 min; pressure, 15 mpa and modifier volume, 35 ml. the essential oil extracts were analyzed by capillary gas chromatography with mass spectrometric detector (gc-ms). the obtained results were compared with references
ESSENTIAL OIL FROM MARCHANTIA CONVOLUTA: EXTRACTION AND COMPONENTS  [cached]
HUI CAO,MEI JUAN JI,HONG XIAN WANG
Journal of the Chilean Chemical Society , 2007,
Abstract: The essential oil of Marchantia convoluta was obtained by supercritical fluid extraction (SFE) using methanol as a modifier. The effects of different parameters, such as pressure, temperature, modifier volume and extraction time, on the extraction of essential oil from M. convoluta were investigated. Maximum global yields were obtained using the following conditions: extraction temperature, 40 oC; dynamic time, 40 min; pressure, 15 Mpa and modifier volume, 35 ml. The essential oil extracts were analyzed by capillary gas chromatography with mass spectrometric detector (GC-MS). The obtained results were compared with references
Supercritical fluid CO2 extraction of essential oil from Marchantia convoluta: global yields and extract chemical composition
Xiao,Jian Bo; Chen,Jing Wen; Xu,Ming;
Electronic Journal of Biotechnology , 2007,
Abstract: the essential oil of marchantia convoluta was obtained by supercritical (carbon dioxide) extraction using methanol as a modifier. global yields were determined according to the orthogonal design. the effects of different parameters, such as pressure, temperature, modifier volume and extraction time, on the supercritical fluid extraction (sfe) of essential oil from m. convoluta were investigated. maximum global yields were obtained using the following conditions: extraction temperature, 35oc; dynamic time, 35 min; pressure, 15 mpa and modifier volume, 40 ml. the essential oil extract was analyzed by capillary gas chromatography with mass spectrometric detector (gc-ms). the compounds were identified according to their retention indices and mass spectra (ei, 70 ev). the results from gc-ms and literature were compared.
Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells
Xiao, J.B.;Chen, X.Q.;Zhang, Y.W.;Jiang, X.Y.;Xu, M.;
Brazilian Journal of Medical and Biological Research , 2006, DOI: 10.1590/S0100-879X2006000600005
Abstract: the cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol) from a plant used in folk medicine, marchantia convoluta, to human non-small cell lung carcinoma (h1299) and liver carcinoma (hepg2) cell lines was tested. after 72-h incubation of lung and liver cancer cell cultures with varying concentrations of extracts (15 to 200 μg/ml), cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and reported in terms of cell viability. the extracts that showed a significant cytotoxicity were subjected to gas chromatography-mass spectrometry analysis to identify the components. the ethyl acetate, but not the petroleum ether or n-butanol extract, had a significant cytotoxicity against lung and liver carcinoma cells with ic50 values of 100 and 30 μg/ml, respectively. a high concentration of ethyl acetate extract (100 μg/ml) rapidly reduced the number of h1299 cells. at lower concentrations of ethyl acetate extract (15, 30, and 40 μg/ml), the numbers of hepg2 cells started to decrease markedly. gas chromatography-mass spectrometry analysis of the ethyl acetate extract revealed the presence of several compounds such as phytol (23.42%), 1,2,4-tripropylbenzene (13.09%), 9-cedranone (12.75%), ledene oxide (7.22%), caryophyllene (1.82%), and caryophyllene oxide (1.15%). hplc analysis result showed that there were no flavonoids in ethyl acetate extract, but flavonoids are abundant in n-butanol extract. further studies are needed regarding the identification, toxicity, and mechanism of action of active compounds.
Cytotoxicity of Marchantia convoluta leaf extracts to human liver and lung cancer cells  [cached]
Xiao J.B.,Chen X.Q.,Zhang Y.W.,Jiang X.Y.
Brazilian Journal of Medical and Biological Research , 2006,
Abstract: The cytotoxicity of three extracts (petroleum ether, ethyl acetate and n-butanol) from a plant used in folk medicine, Marchantia convoluta, to human non-small cell lung carcinoma (H1299) and liver carcinoma (HepG2) cell lines was tested. After 72-h incubation of lung and liver cancer cell cultures with varying concentrations of extracts (15 to 200 μg/mL), cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and reported in terms of cell viability. The extracts that showed a significant cytotoxicity were subjected to gas chromatography-mass spectrometry analysis to identify the components. The ethyl acetate, but not the petroleum ether or n-butanol extract, had a significant cytotoxicity against lung and liver carcinoma cells with IC50 values of 100 and 30 μg/mL, respectively. A high concentration of ethyl acetate extract (100 μg/mL) rapidly reduced the number of H1299 cells. At lower concentrations of ethyl acetate extract (15, 30, and 40 μg/mL), the numbers of HepG2 cells started to decrease markedly. Gas chromatography-mass spectrometry analysis of the ethyl acetate extract revealed the presence of several compounds such as phytol (23.42%), 1,2,4-tripropylbenzene (13.09%), 9-cedranone (12.75%), ledene oxide (7.22%), caryophyllene (1.82%), and caryophyllene oxide (1.15%). HPLC analysis result showed that there were no flavonoids in ethyl acetate extract, but flavonoids are abundant in n-butanol extract. Further studies are needed regarding the identification, toxicity, and mechanism of action of active compounds.
RP-HPLC-DAD detrmination of flavonoids: separation of quercetin, luteolin and apigenin in Marchantia Convoluta
Xiao-qing Chen,Jian-bo Xiao
Iranian Journal of Pharmaceutical Research , 2005,
Abstract: High performance liquid chromatography coupled with photodiode array detector (HPLCDAD)has been reported to quantify isolated flavonoids or these compounds in complex biological matrices, such as Chinese herbal drugs and products from factories. This work was designed, therefore, to develop an HPLC-DAD system to separate quercetin, luteolin and apigenin and to quantify them in extractive solutions from Marchantia convoluta. Flavonoids were analyzed on a Kromasil RP-C18 column; using a mobile phase, consisted of methanolacetonitrile-acetic acid-phosphoric acid-H2O (200:100:10:10:200, V/V); under the following conditions: detecting wavelength, 352 nm; flow rate, 0.60 ml/min; the sensitivity, 0.05 AUFS and the volume of injecting sample, 6.0 μl. The HPLC system was operated at ambient temperature (28±1°C). The method showed linearity for quercetin, luteolin and apigenin in the range 2.0-20.8, 2.2-24.0 and 1.6-20.0 μg/ml respectively, and the R.S.D. of the slope of the three lines was, respectively, 0.33%, 1.21% and 2.49% for quercetin, luteolin and apigenin.The aqueous and ethanol 80% extractive solutions showed linear response 1.5-15 μl/ml and ethanol 50% extractive solution in range 1.0-10 μl/ml. Precision and accuracy were determined for ethanol 80% extractive solution, in concentration of 10 μl/ml. The recoveries were 95.92-98.10%, 92.18-95.13% and 98.72-103.19% for quercetin, luteolin and apigenin respectively.RSD of results was 2.83-3.62%. The HPLC method showed an excellent performance in separating the flavonoids quercetin, luteolin and apigenin in Marchantia convoluta extracts, since the presence of interference has been previously evaluated and the mobile phase waschose carefully.
Supercritical fluid extraction of volatile and non-volatile compounds from Schinus molle L.
Barroso, M. S. T.;Villanueva, G.;Lucas, A. M.;Perez, G. P.;Vargas, R. M. F.;Brun, G. W.;Cassel, E.;
Brazilian Journal of Chemical Engineering , 2011, DOI: 10.1590/S0104-66322011000200015
Abstract: schinus molle l., also known as pepper tree, has been reported to have antimicrobial, antifungal, anti-inflammatory, antispasmodic, antipyretic, antitumoural and cicatrizing properties. this work studies supercritical fluid extraction (sfe) to obtain volatile and non-volatile compounds from the aerial parts of schinus molle l. and the influence of the process on the composition of the extracts. experiments were performed in a pilot-scale extractor with a capacity of 1 l at pressures of 9, 10, 12, 15 and 20 mpa at 323.15 k. the volatile compounds were obtained by co2 supercritical extraction with moderate pressure (9 mpa), whereas the non-volatile compounds were extracted at higher pressure (12 to 20 mpa). the analysis of the essential oil was carried out by gc-ms and the main compounds identified were sabinene, limonene, d-germacrene, bicyclogermacrene, and spathulenol. for the non-volatile extracts, the total phenolic content was determined by the folin-ciocalteau method. moreover, one of the goals of this study was to compare the experimental data with the simulated yields predicted by a mathematical model based on mass transfer. the model used requires three adjustable parameters to predict the experimental extraction yield curves.
Volatile phytochemical composition of rhizome of ginger after extraction by headspace solid-phase microextraction, petroleum ether extraction and steam distillation extraction
Zhannan Yang, Weiping Yang, Quancai Peng, Qiansong He, Yong Feng, Shiqiong Luo, Zhengwen Yu
Bangladesh Journal of Pharmacology , 2009,
Abstract: The sampling techniques headspace solid-phase microextraction (HS-SPME), petrol ether extraction (PEE) and steam distillation extraction (SDE) were compared for the GC-MS of volatile constituents present in ginger (Zingiber officinale). The effects of different parameters, such as extraction fibers, extraction time, extraction temperature and particle size ranges, on the HS-SPME of rhizome of ginger were investigated. Zingiberene (53.12%) were predominant components of ginger samples obtained by HS-SPME whereas those levels were 39.01% in the same samples by PEE and 35.05% in those by SDE, respectively. HS-SPME with polydimethylsiloxane (PDMS) fiber was more selective and particularly efficient for the isolation of volatile phytochemical composition and afforded a higher yield of total compounds than PEE and SDE. The specific compound isolated by SPME, which due to effective fiber, was much larger than that isolated by PEE or SDE. HS-SPME is a powerful tool for determining the volatile constitutes present in the traditional Chinese medicines.
Rapid and Simple Extraction Method for Volatile N-Nitrosamines in Meat Products  [PDF]
Mohammad Al-Kaseem, Zaid Al-Assaf, Franswa Karabet
Pharmacology & Pharmacy (PP) , 2013, DOI: 10.4236/pp.2013.48087
Abstract: A new methodology for extraction, pre-concentration and analysis of volatile nitrosamines in meat-derived products was developed and compared with conventional methods (Distillation and two-step solid-phase extraction). The samples (canned sausages, cured meat, luncheon and smoked meat) were treated with an aqueous sodium hydroxide (NaOH) by autoclaving at 121°C for 10 min and extracted by liquid-liquid extraction with dichloromethane, then the nitrosamines were pre-concentrated using activated silica. Then, gas chromatography coupled with flame ionization detector was used for the separation and determination of the different nitrosamines contained in a real sample and gas chromatography with mass spectrometry detection was used as the confirmation technique. The newly invented autoclaving method allowed the determination of nitrosamine compounds at trace levels with limit of detection ranged from 0.077 to 0.18 ppb and quantitation limits were from 0.26 to 0.6 ppb for all nitrosamines, and found to be superior to the conventional ones, yielding approximately about 10%-20% increasing in the recovery compared with the mean recovery obtained when applying conventional methods.
Study on Optimal Extraction Technology for Volatile Oil in Radix Angelicae Sinensis
当归挥发油提取工艺的研究

Yang Rongping,Yan Qianru,Wang Yunhong,Wang Miao,Huang Juan,Han Li,
杨荣平
,严倩茹,王云红,王淼,黄娟,韩丽

世界科学技术-中医药现代化 , 2012,
Abstract: This study was aimed to optimize the extraction of volatile oil in Radix Angelicae Sinensis. The L9(34) orthogonal design was carried out for the optimization of water extraction of volatile oil in Radix Angelicae Sinensis. And the content of volatile oil was used as reference index. The results showed that the optimal extraction process was as follows. Medicinal materials were crashed to go through mesh 10, then were soaked in 10-fold volume of water for 3 hours, and were extracted for 8 hours. It was concluded that the optimal extraction technology is simple and feasible with the extraction rate of volatile oil being 5.0%.
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