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Isolation, Partial Purification, and Characterization of Lectins from the Roots of Vigna unguiculata (L.) Walp.  [cached]
Gilda Rivero,Reginald Camillo,Ernelea Cao,Remedios Roderos
Science Diliman , 1995,
Abstract: A lectin was isolated from the roots of 7-day old Vigna unguiculata (L.) Walp. seedlings using size-exclusion chromatography. The root lectin revealed two subunits in SDS-PAGE and migrated as a single protein in a pH gradient isoelectrofocusing gel electrophoresis as well as in native gel electrophoresis. The root lectin exhibited no specificity to any of the erythrocytes tested. In addition, the agglutinating activity of the root lectin was markedly increased by enzymes. The hemeagglutinating activity of the root lectin was inhibited by the sugars N-acetyl-galactosamine and methyl-a-D-glucopyranoside.
Anatomical Studies On The Roots Of Some Vigna Savi Species (Leguminosae Papilionoideae)
F.N. Mbagwu,H.O. Edeoga
Agricultural Journal , 2013,
Abstract: Anatomical studies on the roots of eight Vigna sp. namely V. ambacensis, V. gracillis, V. racemosa, V. reticulata, V. subterranea, V. triloba, V. unguiculata and V. vexillata are reported in this study. An analysis of the features of the internal arrangement of the roots shows that these taxa possess vital taxonomic characters that could be used to establish interspecific relationships among these taxa. An interesting aspect of the root anatomy of these taxa is the presence of 3 prominent epidermal hairs in V. vexillata which distinguished this taxon from the rest of the taxa studied. Their vessels varied in size and number, they are small and numerous in V. subterranea, V. ambacensis and V. reticulata but big and few in V. gracillis, V. vexillata and V. unguiculata. In this studied, there is concentration of vascular bundles at the central part of the root cortex hence the endodermis is not clearly differentiated. V. subterranea is characterized with crystal sand and two or three vessels that appear together at different locations.
PHYTOCHEMICAL PROPERTIES AND ANTHELMINTIC ACTIVITY OF VIGNA UNGUICULATA LINN.  [PDF]
Maisale A B,Patil M B,Jalalpure S S,Patil A M
Journal of Pharmaceutical and Scientific Innovation , 2012,
Abstract: Vigna unguiculata Linn belonging to family Fabaceae are used traditionally as appetizer, diuretic, laxative, anthelmintic. Seeds are coarse powdered and exhaustively with hot solvent (Soxhlet) extraction by ethanol and maceration with chloroform water I.P. Five concentrations (10-100 mg/ml) of ethanolic and aqueous extracts were studied for anthelmentic activity by using Eudrilus euginiae earthworms. Both aqueous and ethanolic extracts showed paralysis and death of worms in concentration (10-100 mg/ml) dependent manner. Alcoholic extract of Vigna unguiculata showed significant activity than aqueous extract. Piperazine citrate (10 mg/ml) and distilled water were included in the assay as standard drug and control respectively. The result showed seeds of vigna unguiculata possessed potential anthelmintic activity. The seeds extract also showed presence of flavonoids, and glycosides by preliminary phytochemical investigations.
Computational Identification of Novel MicroRNAs and Their Targets in Vigna unguiculata  [PDF]
Yongzhong Lu,Xiaoyun Yang
Comparative and Functional Genomics , 2010, DOI: 10.1155/2010/128297
Abstract: MicroRNAs (miRNAs) are a class of endogenous, noncoding, short RNAs directly involved in regulating gene expression at the posttranscriptional level. High conservation of miRNAs in plant provides the foundation for identification of new miRNAs in other plant species through homology alignment. Here, previous known plant miRNAs were BLASTed against the Expressed Sequence Tag (EST) and Genomic Survey Sequence (GSS) databases of Vigna unguiculata, and according to a series of filtering criteria, a total of 47 miRNAs belonging to 13 miRNA families were identified, and 30 potential target genes of them were subsequently predicted, most of which seemed to encode transcription factors or enzymes participating in regulation of development, growth, metabolism, and other physiological processes. Overall, our findings lay the foundation for further researches of miRNAs function in Vigna unguiculata.
Alterations in Lipid Peroxidation and Some Antioxidant Enzymes in Germinating Beans(Vigna unguiculata) and Maize (Zea mays) Exposed to Nickel
G.E. Eriyamremu,O. Lolodi
International Journal of Botany , 2010,
Abstract: The growth pattern, chlorophyll content, lipid peroxidation as well as the activities of superoxide dismutase (SOD), Glutathione Peroxidases (GP) and catalase (CAT) were studied in the radicles, roots and shoots of Vigna unguiculata (the common beans) and Zea mays (maize) grown in soils contaminated with two nickel concentrations {0.1% (65 μM) and 0.2% (130 μM)}. Two hundred and seventy of either bean or maize seeds were germinated in these contaminated soils and 10 of each germinated plants were harvested after either the 7th, 14th and 21st days. The study observed chlorosis and stunted growth in the nickel-treated plants. Nickel accumulated in the tissues in increasing order of the radicle, root and the shoot. Treatment of the plants with 0.1% or 0.2% nickel did significantly (p<0.05) affected the activities of SOD, CAT and GP in the tissues studied throughout the experimental periods. Lipid peroxidation as measured by the production of malondialdehyde was significantly increased (p<0.05) in the plants grown on nickel-contaminated soils compared with the controls. These results indicate that nickel cause oxidative damage in Vigna unguiculata and Zea mays and as an adaptive feature, they increase the activities of antioxidant enzymes of the radicle, root and shoot.
Induced mutations in cowpea, Vigna unguiculata (Leguminosae)
Odeigah,P.G.C.; Osanyinpeju,A.O.; Myers,G.O.;
Revista de Biología Tropical , 1998,
Abstract: two cowpea (vigna unguiculata l. walp) varieties, it84e-124 and vita 7 of the international institute of tropical agriculture, ibadan, were exposed to varying doses of chemical and physical mutagens. optimum doses of 10mm ems for 6hr and 0.1mm and 1.0mm nan3 for 2hr, determined from seeding growth tests and 100r and 200r gamma radiations were applied to 2000 seed samples of each genotype. screening of the m2 generation revealed that the mutagenic treatments induced morphological, physiological and biochemical changes in the genotypes. a spectrum of mutations which included variants with respect to anthocyanin pigmentation, leaf morphology, maturity date, male sterility and insect pest resistance qualities were observed. lines with significant increases in yield parameters such as number of seeds per pod, peduncles per plant, 100 seed weight and seed storage proteins were selected.
Induced mutations in cowpea, Vigna unguiculata (Leguminosae)
P.G.C. Odeigah,A.O. Osanyinpeju,G.O. Myers
Revista de Biología Tropical , 1998,
Abstract: Two cowpea (Vigna unguiculata L. Walp) varieties, IT84E-124 and Vita 7 of the International Institute of Tropical Agriculture, Ibadan, were exposed to varying doses of chemical and physical mutagens. Optimum doses of 10mM EMS for 6hr and 0.1mM and 1.0mM NaN3 for 2hr, determined from seeding growth tests and 100R and 200R gamma radiations were applied to 2000 seed samples of each genotype. Screening of the M2 generation revealed that the mutagenic treatments induced morphological, physiological and biochemical changes in the genotypes. A spectrum of mutations which included variants with respect to anthocyanin pigmentation, leaf morphology, maturity date, male sterility and insect pest resistance qualities were observed. Lines with significant increases in yield parameters such as number of seeds per pod, peduncles per plant, 100 seed weight and seed storage proteins were selected.
Computational Identification of Novel MicroRNAs and Their Targets in Vigna unguiculata  [PDF]
Yongzhong Lu,Xiaoyun Yang
International Journal of Genomics , 2010, DOI: 10.1155/2010/128297
Abstract: MicroRNAs (miRNAs) are a class of endogenous, noncoding, short RNAs directly involved in regulating gene expression at the posttranscriptional level. High conservation of miRNAs in plant provides the foundation for identification of new miRNAs in other plant species through homology alignment. Here, previous known plant miRNAs were BLASTed against the Expressed Sequence Tag (EST) and Genomic Survey Sequence (GSS) databases of Vigna unguiculata, and according to a series of filtering criteria, a total of 47 miRNAs belonging to 13 miRNA families were identified, and 30 potential target genes of them were subsequently predicted, most of which seemed to encode transcription factors or enzymes participating in regulation of development, growth, metabolism, and other physiological processes. Overall, our findings lay the foundation for further researches of miRNAs function in Vigna unguiculata. 1. Introduction MicroRNAs (miRNAs) are a class of endogenous, small, noncoding, single-stranded RNAs that act as posttranscriptional regulators in eukaryotes [1]. They have been reported to be located mostly within noncoding regions of genomes, and usually transcribed from RNA polymerase II promoters [2, 3]. The generation of mature miRNA is a complicated enzyme-catalyzed process, from the initial transcript pri-miRNA to the precursor (pre-miRNA) with a characteristic hairpin structure, then a miRNA duplex (miRNA?:?miRNA*) [4]. In the end, it is assembled to the RNA-induced silencing complex (RISC) to direct its activity on a target mRNA, depending on the degree of base-pairing between the miRNA and the responsive element and results in either cleavage or translational repression of the target mRNA. Perfect complementarity generally results in cleavage, such as in plants, whereas imperfect base-pairing leads to translational repression [4, 5]. MiRNA genes represent about 1%-2% of the known eukaryotic genomes and constitute an important class of fine-tuning regulators that are involved in several physiological or disease-associated cellular processes [6]. For example, studies in plants have revealed the key roles of miRNAs in diverse regulatory pathways, including growth, development, and defense response against every sort of stress [7–17]. Considering the importance of miRNAs in gene regulation, two major categories of approaches have been applied for miRNA investigation [1]. Compared to the experimental approaches, computation (bioinformatics) methods have been proved to be faster, more affordable, and more effective, contributing mostly to today’s plentiful storage
Differential responses of ribulose-1,5-bisphosphate carboxylase/oxygenase activities of two Vigna unguiculata cultivars to salt stress
Arag?o, Maria Erivalda Farias de;Guedes, Marjorie Moreira;Otoch, Maria de Lourdes Oliveira;Guedes, Maria Izabel Florindo;Melo, Dirce Fernandes de;Lima, Maria da Guia Silva;
Brazilian Journal of Plant Physiology , 2005, DOI: 10.1590/S1677-04202005000200003
Abstract: vita 3 and vita 5 are two vigna unguiculata cultivars that differ in their capacities for survival in saline environments; vita 3 is more tolerant and vita 5 more sensitive. both cultivars were submitted to salt stress with 0.1 m nacl. after 8 days, root and shoot growth from both cultivars was reduced but reduction was more pronounced in vita 5. furthermore, leaf area was also reduced in this cultivar. chlorophyll content and chlorophyll fluorescence parameters were not affected by salt stress, but the specific activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) decreased in vita 3 and increased in vita 5. the use of immunological techniques also revealed that the rubisco content from vita 3 decreased while that of vita 5 increased. the discussion of these results is aimed at reaching a better understanding of the differences between these cultivars in relation to salt stress.
Salt Stress responses on Protein Profile in Vigna unguiculata L.  [PDF]
Johnson M,Dooslin Mary D,Babu A
Journal of Stress Physiology & Biochemistry , 2011,
Abstract: The present study was aimed to elucidate the salt tolerant, salt inducible and salt sensitive protein of the Vigna unguiculata by Sodium Deodyl Sulphate – Poly Acrylamide Gel Electrophoresis. Seedlings of Vigna unguiculata exposed to different environmental conditions exhibited a plethora of physio-chemical responses. The seedlings treated with various concentrations of NaCl at third day showed maximum of 85 bands, with nine active regions and their MW-Rf values ranged from 0.012 to 0.891. The seedlings treated with same experimental set up at fifth day showed maximum number of 63 bands with eight active regions and their MW-Rf values ranged from 0.108 to 0.891. On 5th day seedlings showed the isoperoxidase expression with various sizes of bands. The soluble protein showed a gradual increase during the initial stage and after fifth day there was gradual decrease in their content.
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