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Classification of Pepper (Capsicum annuum L.) Accessions by RAPD Analysis
Kassinee Sitthiwong,Toshiyuki Matsui,Sutevee Sukprakarn
Biotechnology , 2005,
Abstract: Ten pepper (Capsicum annuum L.) germplasm accessions from Thailand were screened using random amplified polymorphic DNA (RAPD) markers. Twelve dodecamer oligonucleotide primers, singly and in combination were used. The similarity values among the studied genotypes range from 0.209 to 0.891. The resulting dendrogram divided the accessions into two major groups. The first group which include 8 accessions with white corolla was further divided into two subgroups. The first subgroup included 4 long-fruited (CA849, CA958, CA1107 and CA1118) and 1 medium-fruited (CA365). The second subgroup included 1 medium-fruited (CA367) and 1 short-fruited (CA398). However, the medium-fruited CA020 could not be clustered into any subgroup. The second group included a short-fruited with 1 white having green margin corolla (CA024) and a yellow green with green-yellow spots corolla (CA034). The obtained clustering based on RAPD markers was consistent with morphological characteristics of the different pepper accessions.
Phytochemical Evaluation of Wild and Cultivated Pepper (Capsicum annuum L. and C. pubescens Ruiz & Pav.) from Oaxaca, Mexico
Vera-Guzmán,Araceli Minerva; Chávez-Servia,José Luis; Carrillo-Rodríguez,José Cruz; G. López,Mercedes;
Chilean journal of agricultural research , 2011, DOI: 10.4067/S0718-58392011000400013
Abstract: reports of the last decade show that some types of food and spices included in the human diet, such as pepper (capsicum annuum l.) can have a positive effect on human health. the mexican pepper germplasm is poorly documented with regard to variety and the amount of phytochemical compounds that it contains. in the present study, the variation of phytochemical compounds was evaluated in nine fruit variants (morphotypes) of wild and cultivated pepper grown in oaxaca. anova detected significant differences among pepper morphotypes and ripeness stages of fruits; vitamin c, total phenols, flavonoids, p-carotene, coordinated chromatic of color, and capsaicinoids. the highest values of vitamin c were found in 'tabaquero', 'guero' and 'coste?o' morphotypes (151.6 to 183.2 mg 100 g-1). with regard to total phenols and flavonoids, 'piquín' and 'solterito' had the highest levels. coordinates of color a* and b*, and chroma presented a positive correlation with phenol and flavonoid contents. the evaluated morphotypes differed in capsaicin and dihydrocapsaicin; c. annuum had higher capsaicin content (4.9 to 142 /
Phytochemical Evaluation of Wild and Cultivated Pepper (Capsicum annuum L. and C. pubescens Ruiz & Pav.) from Oaxaca, Mexico Evaluación Fitoquímica en Chile (Capsicum annuum L. and C. pubescens Ruiz & Pav.) Silvestre y Cultivado en Oaxaca, México  [cached]
Araceli Minerva Vera-Guzmán,José Luis Chávez-Servia,José Cruz Carrillo-Rodríguez,Mercedes G. López
Chilean Journal of Agricultural Research , 2011,
Abstract: Reports of the last decade show that some types of food and spices included in the human diet, such as pepper (Capsicum annuum L.) can have a positive effect on human health. The Mexican pepper germplasm is poorly documented with regard to variety and the amount of phytochemical compounds that it contains. In the present study, the variation of phytochemical compounds was evaluated in nine fruit variants (morphotypes) of wild and cultivated pepper grown in Oaxaca. ANOVA detected significant differences among pepper morphotypes and ripeness stages of fruits; vitamin C, total phenols, flavonoids, P-carotene, coordinated chromatic of color, and capsaicinoids. The highest values of vitamin C were found in 'Tabaquero', 'Guero' and 'Coste o' morphotypes (151.6 to 183.2 mg 100 g-1). With regard to total phenols and flavonoids, 'Piquín' and 'Solterito' had the highest levels. Coordinates of color a* and b*, and chroma presented a positive correlation with phenol and flavonoid contents. The evaluated morphotypes differed in capsaicin and dihydrocapsaicin; C. annuum had higher capsaicin content (4.9 to 142 /En la última década, se reportó que el consumo de ciertos alimentos y especias, como el chile (Capsicum annuum L.), pueden tener un efecto positivo en la salud. Particularmente, los acervos genéticos mexicanos de chile están poco documentados en relación a la diversidad desde la perspectiva fitoquímica. En este trabajo se evaluó la variación de compuestos fitoquímicos en nueve morfotipos de chile silvestres y cultivados de Oaxaca. El ANDEVA detectó diferencias significativas entre morfotipos y estados de madurez en vitamina C, fenoles, flavonoides, P-caroteno, color, y capsaicinoides. Los valores más altos de vitamina C se determinaron en 'Tabaquero', 'Guero' y 'Coste o' (151.6 a 183.2 mg 100 g-1). En fenoles y flavonoides sobresalieron los tipos 'Piquin' y 'Solterito'. Las coordenadas cromáticas a* y b*, y los tonos (C*) se correlacionaron positivamente con el contenido de fenoles totales y flavonoides. Los morfotipos evaluados difieren en el contenido de capsaicina y dihidrocapsaicina, en C. annuum fue mayor la cantidad de capsaicina (4.9 a 142 pig mL-1) que de dihidrocapsaicina (1.5 a 65.5 pg mL-1) y en C. pubescens Ruiz & Pav. el patrón fue inverso.
Pepper EST database: comprehensive in silico tool for analyzing the chili pepper (Capsicum annuum) transcriptome
Hyun-Jin Kim, Kwang-Hyun Baek, Seung-Won Lee, JungEun Kim, Bong-Woo Lee, Hye-Sun Cho, Woo Taek Kim, Doil Choi, Cheol-Goo Hur
BMC Plant Biology , 2008, DOI: 10.1186/1471-2229-8-101
Abstract: We built the Pepper EST database to mine the complexity of chili pepper ESTs. The database was built on 122,582 sequenced ESTs and 116,412 refined ESTs from 21 pepper EST libraries. The ESTs were clustered and assembled into virtual consensus cDNAs and the cDNAs were assigned to metabolic pathway, Gene Ontology (GO), and MIPS Functional Catalogue (FunCat). The Pepper EST database is designed to provide a workbench for (i) identifying unigenes in pepper plants, (ii) analyzing expression patterns in different developmental tissues and under conditions of stress, and (iii) comparing the ESTs with those of other members of the Solanaceae family. The Pepper EST database is freely available at http://genepool.kribb.re.kr/pepper/ webcite.The Pepper EST database is expected to provide a high-quality resource, which will contribute to gaining a systemic understanding of plant diseases and facilitate genetics-based population studies. The database is also expected to contribute to analysis of gene synteny as part of the chili pepper sequencing project by mapping ESTs to the genome.Pepper is a member of the family Solanaceae, which is one of the largest families in the plant kingdom and includes more than 3,000 species [1]. The Solanaceae family includes important crops, such as pepper, tomato, tobacco, potato, and eggplant and has been highly cultivated over the years for human nutrition and health. Capsicum species are consumed worldwide and are valued because of their unique color, pungency, and aroma. Capsicum peppers include C. annuum, C. chinense, C. baccatum, C. frutescens, and C. pubescens and are cultivated in different parts of the world. Of these, the varieties of the chili pepper plant species C. annuum, having a modest-sized diploid genome (2n = 24), are the most heavily consumed due to their nutritional value and spicy taste [2]. The chemical that is primarily responsible for the pungency of C. annuum has been identified as capsaicin [3], which elicits numerous b
Registro de Plagiometriona clavata (Fabricius 1798) (Coleoptera: Chrysomelidae) en chile silvestre Capsicum annuum, de Baja California Sur, México
TEJAS, R. A.;SERVíN, R.;NIETO–GARIBAY, A.;MARíN, A.;
Acta zoológica mexicana , 2011,
Abstract: plagiometriona clavata fabricius, 1798 is reported for the first time on capsicum annuum var. aviculare, considerated like the wild progenitor of the cultivated chiles.
THE EFFECT OF AGRO-ECOLOGICAL ZONES ON THE INCIDENCE AND DISTRIBUTION OF APHID VECTORS OF PEPPER VEINAL MOTTLE VIRUS, ON CULTIVATED PEPPER (CAPSICUM ANNUUM L.) IN NIGERIA  [PDF]
A.A. FAJINMI,C.A. ODEBODE,O.B. FAJINMI
Journal of Central European Agriculture , 2011, DOI: 10.5513/jcea01/12.3.951
Abstract: The distribution of aphid vectors of Pepper veinal mottle virus (PVMV) was studied on cultivated pepper between 2003 and 2005 in the major pepper producing areas of the six agro-ecological zones in Nigeria. The aphids were isolated, identified and their transmission ability determined. Population of aphid vectors increased progressively in all the agro-ecological zones from March at the onset of raining season reaching a peak in August and then declining from September at the on set of the dry season. The Humid forest and Derived Savanna agro-ecological zones recorded highest mean population of aphids / leaf and types of aphid’s species compared with other agro-ecological zones. Six species of aphid, Myzus persicae (Sulzer), Aphis gossipii (Glover), Aphis craccivora (Koch), Aphis spiraecola (Patch), Aphis fabae (Blanchard), and Rhopalosiphum maidis (Fitch), were identified on the pepper fields surveyed in all the agro-ecological zones. There was no significant difference in the occurrence of M. persicae, A. gossipii, A. craccivora, and R. maidis while A. spiraecola and A. fabae that varied in occurrence in all the zones. Ability to transmit PVMV to a healthy pepper plants varied in all the identified aphid species but R. maidis was not able to transmit PVMV from infected to a healthy pepper plant. A more sustainable approach to controlling pepper viruses is by targeting the aphid vectors and preventing the vectors from reaching the crops because the aphid vectors which are virus specific are the major means of transmitting virus to healthy plant.
MICROPROPAGATION OF CAPSICUM ANNUUM L
K. SANATOMBI,G. J. SHARMA
Notulae Botanicae Horti Agrobotanici Cluj-Napoca , 2007,
Abstract: An efficient micropropagation protocol was developed for Capsicum annuum L. cv. 'Morok Amuba', an ornamental chilli cultivar using shoo-tip and axillary shoot-tip explants. Multiple shoot buds were induced from shoot-tip explants on MS medium containing cytokinins alone or in combination with IAA. A maximum number of shoot buds was induced on MS medium containing 10 mg/l Zea followed by 5 mg/l BAP in combination with 1 mg/l IAA. Rooting and elongation of the shoot buds were achieved on MS medium supplemented with 0.5 mg/l IAA or IBA. Axillary shoots were induced on the rooted plantlets by decapitation and the axillary shoot-tips explants were used for further induction of shoot buds by culturing them on a medium containing combinations of BAP with IAA. The shoot buds were rooted on a medium containing 0.5 mg/l IBA. The plantlets showed 80-90% survival during transplantation.
Characterization of Different Capsicum Varieties by Evaluation of Their Capsaicinoids Content by High Performance Liquid Chromatography, Determination of Pungency and Effect of High Temperature  [PDF]
Alberto González-Zamora,Erick Sierra-Campos,J. Guadalupe Luna-Ortega,Rebeca Pérez-Morales,Juan Carlos Rodríguez Ortiz,José L. García-Hernández
Molecules , 2013, DOI: 10.3390/molecules181113471
Abstract: The chili pepper is a very important plant used worldwide as a vegetable, as a spice, and as an external medicine. In this work, eight different varieties of Capsicum annuum L. have been characterized by their capsaicinoids content. The chili pepper fruits were cultivated in the Comarca Lagunera region in North of Mexico. The qualitative and quantitative determination of the major and minor capsaicinoids; alkaloids responsible for the pungency level, has been performed by a validated chromatographic procedure (HPLC-DAD) after a preliminary drying step and an opportune extraction procedure. Concentrations of total capsaicinoids varied from a not detectable value for Bell pepper to 31.84 mg g ?1 dried weight for Chiltepín . Samples were obtained from plants grown in experimental field and in greenhouse without temperature control, in order to evaluate temperature effect. Analysis of the two principal capsaicinoids in fruits showed that the amount of dihydrocapsaicin was always higher than capsaicin. In addition, our results showed that the content of total capsaicinoids for the varieties Serrano, Puya, Ancho, Guajillo and Bell pepper were increased with high temperature, while the content of total capsaicinoids and Scoville heat units (SHU) for the varieties De árbol and Jalape?o decreased. However, the pungency values found in this study were higher for all varieties analyzed than in other studies.
Genetic variability of mineral elements concentration in pepper varieties (Capsicum annuum L.)  [PDF]
Krsti? Borivoj ?.,Gvozdenovi? ?uro J.,Nikoli? Nata?a P.
Zbornik Matice Srpske za Prirodne Nauke , 2010, DOI: 10.2298/zmspn1018079k
Abstract: Pepper (Capsicum annuum L.) is one of the major members of the vegetable kingdom. The differences between the individual varieties in mineral nutrition and the uptake of mineral elements have provoked our interest for the investigations of their genotype specificity. The chemical composition of pepper leaves at flowering and at the end of the growing season was analyzed. On the average, the content of total ash was app. 22%, namely 19-20% (soluble) and 2-3% (insoluble) at both stages. The highest accumulation values were obtained with nitrogen, then potassium, calcium and phosphorus, whereas the lowest with sodium. The most remarkable genotypic differences were recorded at the end of the growing season. The results obtained clearly demonstrate the chemical heterogeneity of pepper varieties. This crop may be grown successfully only if specificity of its mineral nutrition is known.
Response of cotyledon explants of Capsicum annuum L. cv. kujawianka to chosen plant growth regulators in in vitro culture
Alicja Fra?,Krystyna Nowak
Acta Societatis Botanicorum Poloniae , 1995, DOI: 10.5586/asbp.1995.001
Abstract: Shoot buds originated directly on cotyledon explants of Capsicum annuum L. cv. Kujawianka, when Linsmaier and Skoog medium was enriched with BAP (2 mg/l). Kinetin (2 mg/l) or kinetin with IAA (1 mg/l + 1 mg/l) induced indirect shoot buds regeneration from callus. Rooting was obtained with explants cultivated on a medium containing NAA (0,5 mg/l). Occurrence of the early stages of differentiation was proved at the histological level.
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