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Brain and hepatic Hsp70 protein levels in heat-acclimated broiler chickens during heat stress
Guerreiro, EN;Giachetto, PF;Givisiez, PEN;Ferro, JA;Ferro, MIT;Gabriel, JE;Furlan, RL;Macari, M;
Revista Brasileira de Ciência Avícola , 2004, DOI: 10.1590/S1516-635X2004000400002
Abstract: in the present study we have investigated the effects of heat acclimation on brain and hepatic hsp70 protein levels and body temperature of broiler chickens in response to gradual heat stress. two groups of broilers were raised up to 47 days of age under distinct temperature conditions: thermoneutral (tn, according to bird age) or hot environmental (hs, 31-33°c). at 46 days of age, the birds reared at high ambient temperature were transferred to thermoneutrality conditions. after 18 h, these birds and the birds reared at thermoneutral temperature were submitted to gradual heat stress in a climatic chamber so that environment temperature was increased from 28 to 40oc at a rate of 2oc/h. colonic temperature was measured using a thermometer sensor probe at each two hours, and hepatic and brain tissues were collected immediately after slaughter in order to assess hsp70 protein level by western blotting analysis. the colonic temperatures of birds reared at high temperature increased steeply during the first 2 h of heat stress (1.06oc/h) and more slowly thereafter (0.59oc/h). broilers reared at thermoneutral temperature showed a small increase in the first 4 h of heat stress (0.18oc/h) and then colonic temperature increased sharply (0.72oc/h). nevertheless, both groups presented similar final colonic temperature by the end of the stress period. hsp70 levels (ng hsp70 μg total protein-1) did not change in the liver or brain of the birds reared at high temperature. on the other hand, both liver and brain hsp70 levels increased significantly during heat stress in the animals reared at thermoneutrality, with a higher expression of this peptide in brain tissue.
Increase in Apoptosis and of the Stress Protein HSP70 in the Mouse Epididymis Produced by the Antiandrogen Flutamide
Bustos Obregón,Eduardo; Esponda,Pedro;
International Journal of Morphology , 2009, DOI: 10.4067/S0717-95022009000200025
Abstract: the general aim of this paper was to characterize some changes induced by androgen receptors blockage in the epithelial cells of the mouse epididymis. the antiandrogen flutamide was injected (10 mg/kg b.w.) to adult male mice which were sacrificed 24h. and 72h. after. controls injected with the vehicle (corn oil) were sacrificed at the same intervals. cryosections were made of the epididymides and examined by the tunel method for quantification of apoptosis and also using immunocytochemistry to visualize the expression of the stress protein hsp70. the highest indexes of apoptosis were observed in the caput epididymis after 72 h. and were of 7.40 cells/1000 in contrast to controls (0.21 cells/1000). hsp70 appeared particularly increased in the caput and cauda epididymis after 72 h. treatment. results indicated that the blockage of androgen receptors induces apoptosis and a hsp70 expression in the principal epithelial cells of the mouse epididymis, and that these changes occur in a region-specific fashion.
AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus
Bustos-Obregón,Eduardo; Esponda,Pedro;
International Journal of Morphology , 2004, DOI: 10.4067/S0717-95022004000100005
Abstract: apoptosis has been largely analyzed in the testis. nevertheless, the epididymis has been scarcely studied. we analyzed the number of apoptotic cells in the different regions (caput, corpus and cauda) of the epididymis of the south american rodent octodon degus both young and senile. apoptosis was identified using the tunel method which detects in situ dna fragmentation. apoptosis was detected in the principal cells of the epididymal epithelium. the caput epididymis was the region more affected. the caput of young animals showed that 0.32/1000 cells were apoptotic in contrast to 5.1/1000 of senile animals. also in the cauda epididymis apoptosis is increased with age, appearing 0.14/1000 and 3.9/1000 in young and senile animals, respectively. on the other hand, we used a immunocytochemical method to localize the stress protein hsp70. hsp70 appeared notably increased in the principal cells of the cauda epididymis of senile animals. changes in the epididymal epithelium are probably due to the low androgen levels existing in senile animals and are a region dependent phenomenon
Increase in Apoptosis and of the Stress Protein HSP70 in the Mouse Epididymis Produced by the Antiandrogen Flutamide El Antiandrógeno Flutamida Produce un Aumento de la Apoptosis y de la Proteína de Stress HSP70 en el Epidídimo del Ratón
Eduardo Bustos Obregón,Pedro Esponda
International Journal of Morphology , 2009,
Abstract: The general aim of this paper was to characterize some changes induced by androgen receptors blockage in the epithelial cells of the mouse epididymis. The antiandrogen flutamide was injected (10 mg/Kg b.w.) to adult male mice which were sacrificed 24h. and 72h. after. Controls injected with the vehicle (corn oil) were sacrificed at the same intervals. Cryosections were made of the epididymides and examined by the TUNEL method for quantification of apoptosis and also using immunocytochemistry to visualize the expression of the stress protein HSP70. The highest indexes of apoptosis were observed in the caput epididymis after 72 h. and were of 7.40 cells/1000 in contrast to controls (0.21 cells/1000). HSP70 appeared particularly increased in the caput and cauda epididymis after 72 h. treatment. Results indicated that the blockage of androgen receptors induces apoptosis and a HSP70 expression in the principal epithelial cells of the mouse epididymis, and that these changes occur in a region-specific fashion. Este trabajo estudia los cambios inducidos por el bloqueador de receptores de andrógeno flutamida en el epitelio del epidídimo del ratón. Varios machos adultos fueron inyectados con flutamida (lOmg/Kg.b.w.) y se sacrificaron a las 24 y 72horas. Otros machos, que sirvieron de controles fueron inyectados sólo con el vehículo empleado para las inyecciones (aceite de maíz) y se sacrificaron a intervalos similares. Los epidídimos tratados y controles fueron examinados mediante el método TúNEL para cuantificar la apoptosis y mediante procedimientos inmunocitoquímicos para localizar la proteína de stress HSP70. El índice apoptótico más alto fue observado en la cabeza del epidídimo después de 72 horas de tratamiento. HSP70 se observó también a las 72 horas en la cabeza y en la cauda epididimaria. Los resultados indican que el bloqueo de los receptores de andrógenos induce apoptosis y expresión de HSP70 en las células principales del epitelio epididimario, y que estos cambios ocurren afectando a regiones específicas del epidídimo.
Mesothelioma Cells Escape Heat Stress by Upregulating Hsp40/Hsp70 Expression via Mitogen-Activated Protein Kinases
Michael Roth,Jun Zhong,Michael Tamm,John Szilard
Journal of Biomedicine and Biotechnology , 2009, DOI: 10.1155/2009/451084
Abstract: Therapy with hyperthermal chemotherapy in pleural diffuse malignant mesothelioma had limited benefits for patients. Here we investigated the effect of heat stress on heat shock proteins (HSP), which rescue tumour cells from apoptosis. In human mesothelioma and mesothelial cells heat stress (39–42°C) induced the phosphorylation of two mitogen activated kinases (MAPK) Erk1/2 and p38, and increased Hsp40, and Hsp70 expression. Mesothelioma cells expressed more Hsp40 and were less sensitive to heat stress compared to mesothelial cells. Inhibition of Erk1/2 MAPK by PD98059 or by Erk1 siRNA down-regulated heat stress-induced Hsp40 and Hsp70 expression and reduced mesothelioma cell survival. Inhibition of p38MAPK by SB203580 or siRNA reduced Hsp40, but not Hsp70, expression and also increased mesothelioma cell death. Thus hyperthermia combined with suppression of p38 MAPK or Hsp40 may represent a novel approach to improve mesothelioma therapy.
The role of the cerebellum in schizophrenia: from cognition to molecular pathways
Yeganeh-Doost, Peyman;Gruber, Oliver;Falkai, Peter;Schmitt, Andrea;
Clinics , 2011, DOI: 10.1590/S1807-59322011001300009
Abstract: beside its role in motor coordination, the cerebellum is involved in cognitive function such as attention, working memory, verbal learning, and sensory discrimination. in schizophrenia, a disturbed prefronto-thalamo-cerebellar circuit has been proposed to play a role in the pathophysiology. in addition, a deficit in the glutamatergic n-methyl-d-aspartate (nmdaf) receptor has been hypothesized. the risk gene neuregulin 1 may play a major role in this process. we demonstrated a higher expression of the nmda receptor subunit 2d in the right cerebellar regions of schizophrenia patients, which may be a secondary upregulation due to a dysfunctional receptor. in contrast, the neuregulin 1 risk variant containing at least one c-allele was associated with decreased expression of nmda receptor subunit 2c, leading to a dysfunction of the nmda receptor, which in turn may lead to a dysfunction of the gamma amino butyric acid (gaba) system. accordingly, from post-mortem studies, there is accumulating evidence that gabaergic signaling is decreased in the cerebellum of schizophrenia patients. as patients in these studies are treated with antipsychotics long term, we evaluated the effect of long-term haloperidol and clozapine treatment in an animal model. we showed that clozapine may be superior to haloperidol in restoring a deficit in nmda receptor subunit 2c expression in the cerebellum. we discuss the molecular findings in the light of the role of the cerebellum in attention and cognitive deficits in schizophrenia.
AGEING INDUCES APOPTOSIS AND INCREASES HSP70 STRESS PROTEIN IN THE EPIDIDYMIS OF Octodon degus EL ENVEJECIMIENTO INDUCE APOPTOSIS Y AUMENTO DE LA PROTEíNA HSP70 EN EL EPIDíDIMO DE Octodon degus  [cached]
Eduardo Bustos-Obregón,Pedro Esponda
International Journal of Morphology , 2004,
Abstract: Apoptosis has been largely analyzed in the testis. Nevertheless, the epididymis has been scarcely studied. We analyzed the number of apoptotic cells in the different regions (caput, corpus and cauda) of the epididymis of the South American rodent Octodon degus both young and senile. Apoptosis was identified using the TUNEL method which detects in situ DNA fragmentation. Apoptosis was detected in the principal cells of the epididymal epithelium. The caput epididymis was the region more affected. The caput of young animals showed that 0.32/1000 cells were apoptotic in contrast to 5.1/1000 of senile animals. Also in the cauda epididymis apoptosis is increased with age, appearing 0.14/1000 and 3.9/1000 in young and senile animals, respectively. On the other hand, we used a immunocytochemical method to localize the stress protein HSP70. HSP70 appeared notably increased in the principal cells of the cauda epididymis of senile animals. Changes in the epididymal epithelium are probably due to the low androgen levels existing in senile animals and are a region dependent phenomenon La apoptosis ha sido muy estudiada en el testículo, no así en el epidídimo. Se analiza el número de células apoptóticas en las diferentes regiones epididimarias (cabeza, cuerpo, cola) del roedor sudamericano Octodon degus joven y senil. La apoptosis se identificó con la técnica de TUNEL que detecta fragmentación del ADN y se la observó en las células epiteliales principales. La cabeza fue el segmento más afectado, con 0.32/1000 células apoptóticas en los animales jóvenes vs 5.1/1000 en seniles. También en la cola la apoptosis aumenta con la edad, con 0.14/1000 y 3.9/1000 en jóvenes y seniles, respectivamente. Por otra parte, la detección inmunohistoquímica de la proteína de estrés termico HSP-70 mostró que ésta aumenta notoriamente en las células principales de la cola epididimaria en animales seniles. Los cambios en el epitelio epididimario se deben probablemente a los bajos niveles de andrógenos del animal senil y son dependientes de la región epididimaria analizada
Application of the stress protein (HSP70) as the biomarker in studying zinc and copper, and the joint toxicity to fish liver
应用应激蛋白HSP70作为生物标志物研究锌、铜及其联合毒性对鲫鱼肝脏的影响

SHEN Hu,WANG Xiaorong,ZHANG Jingfei,
沈骅
,王晓蓉,张景飞

环境科学学报 , 2004,
Abstract: 将应激蛋白 (HSP70 )作为生物标志物 ,以银鲫 (Carassiusauratusgibelio)作为实验生物 ,经过 4 0d不同浓度铜 (Cu2 )、锌(Zn2 )及其混合物暴露后 ,运用SDS PAGE和WesternBlotting方法研究了鲫鱼肝脏组织内应激蛋白HSP70的诱导表达情况 .结果表明 :在实验浓度下 ,Cu2 ,Zn2 及其混合物对鱼肝脏内HSP70有显著的诱导 (P <0 0 5 ) ,而将Cu2 和Zn2 实验组与其混合物实验组相比 ,Cu2 ,Zn2 表现为一定的协同作用 .实验还发现 ,在国家渔业水质标准Zn2 浓度下 ,鱼肝脏组织内HSP70已经有明显的表达 ,说明分子生物学指标要比传统的环境检测指标敏感 ,对污染物具有早期预警的作用 ,将其作为生物标志物来监测环境中的重金属污染具有重要意义
Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stress  [cached]
C Poltronieri,L Maccatrozzo,C Simontacchi,D Bertotto
European Journal of Histochemistry , 2007, DOI: 10.4081/1134
Abstract: In aquaculture, fish are exposed to stressful conditions, which cause an increased synthesis of heat shock proteins (HSPs) at the cellular level. In this work we considered the expression of the constitutive and inducible forms of HSP70 as an indicator of stress caused by transport, during development of the sea bass (Dicentrarchus labrax), a teleost fish of high value for aquaculture. Qualitative RT-PCR analysis revealed expression of inducible HSP70 gene in larvae and fry (25, 40 and 80 days) as well as in adult tissues (liver, brain, muscle, gills, kidney, gonads, heart, spleen and skin) of both control and stressed animals. Expression of inducible HSP70 mRNA examined in different adult tissues by Real- Time PCR, was significantly higher in skin and skeletal muscle of stressed animals than in controls. Immunolocalization of inducible and constitutive forms of heat shock protein 70 (HSP70 and HSC70), reported here for the first time, demonstrated an ubiquitous distribution of HSC70 protein in several tissues of both stressed and control animals (at all stages), while inducible HSP70 protein was found only in skeletal muscle of stressed animals. In all stressed animals, regardless of their developmental stage, cortisol levels were higher than in control animals.
Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds
Mazzi, Carmen Maria;Ferro, Jesus Aparecido;Ferro, Maria Inês Tiraboschi;Savino, Vicente José Maria;Coelho, Antonio Augusto Domingos;Macari, Marcos;
Genetics and Molecular Biology , 2003, DOI: 10.1590/S1415-47572003000300010
Abstract: the promoter region and the beginning of the coding region of the hsp70 stress gene were analysed in broiler chickens of a commercial breed (hubbard-pettersen), a breed selected for weight gain (pp1) and a non-selected breed (naked-neck label rouge). the naked neck gene (naked neck, na), which reduces feathering in birds and is thus related to heat resistance, was present in both pp1 and label rouge breeds. genomic dna was restricted with psti and southern blotting analysis of the samples revealed the absence of polymorphic sites for that enzyme in the promoter region and beginning of the coding region of the hsp70 gene of studied birds. pcr-sscp analysis of these regions, however, indicated the presence of polymorphisms in the beginning of the coding region and the sequencing of the pcr products confirmed and identified two polymorphic sites in this region: a transition a ? g in position +258 and a transversion c ? g in position +276. both mutations were considered to be silent, since they did not modify the aminoacid sequence of the protein hsp70. the promoter region of the hsp70 gene was identical in all studied birds, indicating that the regulation pattern of this gene must be the same in all birds at the promoter region. three different alleles (hsp70-1, hsp70-2 and hsp70-3) were identified for the hsp70 gene from the observed mutations. the allele hsp70-3 was detected in only two breeds, hubbard-pettersen and pp1, but at a low frequency (0,016 and 0,006, respectively).
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